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Article: Protective Effect of Curcumin on D-Galactose-Induced Senescence and Oxidative Stress in LLC-PK1 and HK-2 Cells.

García-Trejo, Semiramis Stephania / Gómez-Sierra, Tania / Eugenio-Pérez, Dianelena / Medina-Campos, Omar Noel / Pedraza-Chaverri, José

Antioxidants (Basel, Switzerland)

2024 Volume 13, Issue 4

Abstract: D-galactose has been widely used as an inducer of cellular senescence and pathophysiological processes related to aging because it induces oxidative stress. On the other hand, the consumption of antioxidants such as curcumin can be an effective strategy ... ...

Abstract	D-galactose has been widely used as an inducer of cellular senescence and pathophysiological processes related to aging because it induces oxidative stress. On the other hand, the consumption of antioxidants such as curcumin can be an effective strategy to prevent phenotypes related to the enhanced production of reactive oxygen species (ROS), such as aging and senescence. This study aimed to evaluate the potential protective effect of curcumin on senescence and oxidative stress and endoplasmic reticulum stress induced by D-galactose treatment in Lilly Laboratories Culture-Porcine Kidney 1 (LLC-PK1) and human kidney 2 (HK-2) proximal tubule cell lines from pig and human, respectively. For senescence induction, cells were treated with 300 mM D-galactose for 120 h and, to evaluate the protective effect of the antioxidant, cells were treated with 5 µM curcumin for 24 h and subsequently treated with curcumin + D-galactose for 120 h. In LLC-PK1 cells, curcumin treatment decreased by 20% the number of cells positive for senescence-associated (SA)-β-D-galactosidase staining and by 25% the expression of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and increased by 40% lamin B1 expression. In HK-2 cells, curcumin treatment increased by 60% the expression of proliferating cell nuclear antigen (PCNA, 50% Klotho levels, and 175% catalase activity. In both cell lines, this antioxidant decreased the production of ROS (20% decrease for LLC-PK1 and 10 to 20% for HK-2). These data suggest that curcumin treatment has a moderate protective effect on D-galactose-induced senescence in LLC-PK1 and HK-2 cells.
Language	English
Publishing date	2024-03-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox13040415
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Article ; Online: Cobalt protoporphyrin modulates antioxidant enzyme activity in the hypothalamus and motor cortex of female rats.

Serrano-García, Norma / Pinete-Sánchez, Raúl / Medina-Campos, Omar Noel / Ramos-Santander, Minerva Adriana / Pedraza-Chaverri, José / Orozco-Ibarra, Marisol

Cellular and molecular biology (Noisy-le-Grand, France)

2024 Volume 70, Issue 4, Page(s) 53–60

Abstract: Cobalt protoporphyrin (CoPP) is a synthetic heme analog that has been observed to reduce food intake and promote sustained weight loss. While the precise mechanisms responsible for these effects remain elusive, earlier research has hinted at the ... ...

Abstract	Cobalt protoporphyrin (CoPP) is a synthetic heme analog that has been observed to reduce food intake and promote sustained weight loss. While the precise mechanisms responsible for these effects remain elusive, earlier research has hinted at the potential involvement of nitric oxide synthase in the hypothalamus. This study aimed to delve into CoPP's impact on the activities of crucial antioxidant enzymes: superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) across seven distinct brain regions (hippocampus, hypothalamus, prefrontal cortex, motor cortex, striatum, midbrain, and cerebellum), as well as in the liver and kidneys. Female Wistar rats weighing 180 to 200 grams received a single subcutaneous dose of 25 μmol/kg CoPP. After six days, brain tissue was extracted to assess the activities of antioxidant enzymes and quantify malondialdehyde levels. Our findings confirm that CoPP administration triggers the characteristic effects of decreased food intake and reduced body weight. Moreover, it led to an increase in SOD activity in the hypothalamus, a pivotal brain region associated with food intake regulation. Notably, CoPP-treated rats exhibited elevated enzymatic activity of catalase, GR, and GST in the motor cortex without concurrent signs of heightened oxidative stress. These results underscore a strong connection between the antioxidant system and food intake regulation. They also emphasize the need for further investigation into the roles of antioxidant enzymes in modulating food intake and the ensuing weight loss, using CoPP as a valuable research tool.
MeSH term(s)	Animals ; Female ; Rats, Wistar ; Hypothalamus/metabolism ; Hypothalamus/drug effects ; Hypothalamus/enzymology ; Antioxidants/metabolism ; Protoporphyrins/pharmacology ; Motor Cortex/drug effects ; Motor Cortex/metabolism ; Motor Cortex/enzymology ; Superoxide Dismutase/metabolism ; Catalase/metabolism ; Rats ; Oxidative Stress/drug effects ; Glutathione Peroxidase/metabolism ; Eating/drug effects ; Glutathione Transferase/metabolism ; Body Weight/drug effects ; Glutathione Reductase/metabolism ; Malondialdehyde/metabolism
Chemical Substances	Antioxidants ; Protoporphyrins ; cobaltiprotoporphyrin (63AAN3JDZE) ; Superoxide Dismutase (EC 1.15.1.1) ; Catalase (EC 1.11.1.6) ; Glutathione Peroxidase (EC 1.11.1.9) ; Glutathione Transferase (EC 2.5.1.18) ; Glutathione Reductase (EC 1.8.1.7) ; Malondialdehyde (4Y8F71G49Q)
Language	English
Publishing date	2024-04-28
Publishing country	France
Document type	Journal Article
ZDB-ID	1161779-2
ISSN	1165-158X ; 0145-5680
ISSN (online)	1165-158X
ISSN	0145-5680
DOI	10.14715/cmb/2024.70.4.9
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Article ; Online: Corrigendum to "Cannabinoid-profiled agents improve cell survival via reduction of oxidative stress and inflammation, and Nrf2 activation in a toxic model combining hyperglycemia+Aβ1-42 peptide in rat hippocampal neurons" [Neurochemistry International 140 (2020) 104817].

Elmazoglu, Zubeyir / Rangel-López, Edgar / Medina-Campos, Omar Noel / Pedraza-Chaverri, José / Túnez, Isaac / Aschner, Michael / Santamaría, Abel / Karasu, Çimen

Neurochemistry international

2023 Volume 171, Page(s) 105616

Language	English
Publishing date	2023-09-28
Publishing country	England
Document type	Published Erratum
ZDB-ID	283190-9
ISSN	1872-9754 ; 0197-0186
ISSN (online)	1872-9754
ISSN	0197-0186
DOI	10.1016/j.neuint.2023.105616
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Article ; Online: α‐Mangostin induces oxidative damage, mitochondrial dysfunction, and apoptosis in a triple‐negative breast cancer model

Cruz‐Gregorio, Alfredo / Aranda‐Rivera, Ana Karina / Aparicio‐Trejo, Omar Emiliano / Medina‐Campos, Omar Noel / Sciutto, Edda / Fragoso, Gladis / Pedraza‐Chaverri, José

Phytotherapy Research. 2023 Aug., v. 37, no. 8 p.3394-3407

2023

Abstract: Triple‐negative breast cancer (TNBC) does not express estrogen receptor, progesterone receptor, and human epidermal growth factor receptor; therefore, TNBC lacks targeted therapy, and chemotherapy is the only available treatment for this illness but ... ...

Abstract	Triple‐negative breast cancer (TNBC) does not express estrogen receptor, progesterone receptor, and human epidermal growth factor receptor; therefore, TNBC lacks targeted therapy, and chemotherapy is the only available treatment for this illness but causes side effects. A putative strategy for the treatment of TNBC could be the use of the polyphenols such as α‐Mangostin (α‐M), which has shown anticancerogenic effects in different cancer models and can modulate the inflammatory and prooxidant state in several pathological models. The redox state, oxidative stress (OS), and oxidative damage are highly related to cancer development and its treatment. Thus, this study aimed to evaluate the effects of α‐M on redox state, mitochondrial metabolism, and apoptosis in 4T1 mammary carcinoma cells. We found that α‐M decreases both protein levels and enzymatic activity of catalase, and increases reactive oxygen species, oxidized proteins and glutathione disulfide, which demonstrates that α‐M induces oxidative damage. We also found that α‐M promotes mitochondrial dysfunction by abating basal respiration, the respiration ligated to oxidative phosphorylation (OXPHOS), and the rate control of whole 4T1 cells. Additionally, α‐M also decreases the levels of OXPHOS subunits of mitochondrial complexes I, II, III, and adenosine triphosphate synthase, the activity of mitochondrial complex I as well as the levels of peroxisome proliferator‐activated receptor‐gamma co‐activator 1α, showing a mitochondrial mass reduction. Then, oxidative damage and mitochondrial dysfunction induced by α‐M induce apoptosis of 4T1 cells, which is evidenced by B cell lymphoma 2 decrease and caspase 3 cleavage. Taken together, our results suggest that α‐M induces OS and mitochondrial dysfunction, resulting in 4T1 cell death through apoptotic mechanisms.
Keywords	B-cell lymphoma ; adenosine triphosphate ; apoptosis ; breast neoplasms ; carcinogenesis ; caspase-3 ; catalase ; disulfides ; drug therapy ; enzyme activity ; epidermal growth factor receptors ; estrogen receptors ; glutathione ; humans ; mitochondria ; models ; oxidative phosphorylation ; oxidative stress ; peroxisome proliferator-activated receptor gamma ; phytotherapy ; polyphenols ; progesterone receptors ; reactive oxygen species ; research
Language	English
Dates of publication	2023-08
Size	p. 3394-3407.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	639136-9
ISSN	1099-1573 ; 0951-418X
ISSN (online)	1099-1573
ISSN	0951-418X
DOI	10.1002/ptr.7812
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Article ; Online: GK-1 Induces Oxidative Stress, Mitochondrial Dysfunction, Decreased Membrane Potential, and Impaired Autophagy Flux in a Mouse Model of Breast Cancer

Cruz-Gregorio, Alfredo / Aranda-Rivera, Ana Karina / Aparicio-Trejo, Omar Emiliano / Medina-Campos, Omar Noel / Sciutto, Edda / Fragoso, Gladis / Pedraza-Chaverri, José

Antioxidants. 2022 Dec. 27, v. 12, no. 1

2022

Abstract: Breast cancer (BC) is the second most common cancer worldwide in women. During the last decades, the mortality due to breast cancer has progressively decreased due to early diagnosis and the emergence of more effective new treatments. However, human ... ...

Abstract	Breast cancer (BC) is the second most common cancer worldwide in women. During the last decades, the mortality due to breast cancer has progressively decreased due to early diagnosis and the emergence of more effective new treatments. However, human epidermal growth factor receptor 2 (HER2) and triple-negative breast cancer (TNBC) remain with poor prognoses. In our research group, we are proposing the GK-1 immunomodulatory peptide as a new alternative for immunotherapy of these aggressive tumors. GK-1 reduced the growth rate of established tumors and effectively reduced lung metastasis in the 4T1 experimental murine model of breast cancer. Herein, the effect of GK-1 on the redox state, mitochondrial metabolism, and autophagy of triple-negative tumors that can be linked to cancer evolution was studied. GK-1 decreased catalase activity, reduced glutathione (GSH) content and GSH/oxidized glutathione (GSSG) ratio while increased hydrogen peroxide (H₂O₂) production, GSSG, and protein carbonyl content, inducing oxidative stress (OS) in tumoral tissues. This imbalance between reactive oxygen species (ROS) and antioxidants was related to mitochondrial dysfunction and uncoupling, characterized by reduced mitochondrial respiratory parameters and dissipation of mitochondrial membrane potential (ΔΨm), respectively. Furthermore, GK-1 likely affected autophagy flux, confirmed by elevated levels of p62, a marker of autophagy flux. Overall, the induction of OS, dysfunction, and uncoupling of the mitochondria and the reduction of autophagy could be molecular mechanisms that underlie the reduction of the 4T1 breast cancer induced by GK-1.
Keywords	animal models ; autophagy ; breast neoplasms ; catalase ; early diagnosis ; erbB-2 receptor ; evolution ; glutathione ; hydrogen peroxide ; immunotherapy ; lungs ; membrane potential ; metabolism ; metastasis ; mice ; mitochondria ; mitochondrial membrane ; mortality ; oxidative stress
Language	English
Dates of publication	2022-1227
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox12010056
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Article ; Online: Tomato lipidic extract plus selenium decrease prostatic hyperplasia, dihydrotestosterone and androgen receptor expression versus finasteride in rats.

Arias-Chávez, David Julian / Mailloux-Salinas, Patrick / Ledesma-Aparicio, Jessica / Campos-Pérez, Elihu / Medina-Campos, Omar Noel / Pedraza-Chaverri, José / Bravo, Guadalupe

World journal of urology

2023 Volume 41, Issue 10, Page(s) 2793–2799

Abstract: Purpose: Evaluate the therapeutic effect of a tomato lipidic extract (STE) in combination with selenium (Se) on rats with prostatic hyperplasia (PH) and to observe its possible mechanisms of action and synergism versus finasteride.: Materials and ... ...

Abstract	Purpose: Evaluate the therapeutic effect of a tomato lipidic extract (STE) in combination with selenium (Se) on rats with prostatic hyperplasia (PH) and to observe its possible mechanisms of action and synergism versus finasteride. Materials and methods: 54 male Wistar rats of nine weeks old were divided in Control (C), PH, Finasteride (F), STE, Se, F + STE, F + Se, STE + Se and F + STE + Se with testosterone enanthate (except C). After 4 weeks of treatment administration, prostate weight, bladder weight, diuresis, prooxidant and antioxidant activity, dihydrotestosterone (DHT), androgen receptor (AR) expression and anatomopathological analysis were determined. Results: STE + Se decreased prostate weight 53.8% versus 28% in F group, also STE + Se decreased significatively glandular hyperplasia, prooxidant activity, DHT and AR expression and increased diuresis and antioxidant activity versus finasteride which increased MDA in prostate. Conclusions: These results demonstrate a greater therapeutic and beneficial effect of tomato lipidic extract in combination with Se in young rats with PH with respect to finasteride without increase prooxidant activity.
MeSH term(s)	Animals ; Male ; Rats ; Androgens/metabolism ; Antioxidants/pharmacology ; Antioxidants/therapeutic use ; Dihydrotestosterone/metabolism ; Finasteride/pharmacology ; Finasteride/therapeutic use ; Prostatic Hyperplasia/drug therapy ; Prostatic Hyperplasia/pathology ; Rats, Wistar ; Receptors, Androgen/metabolism ; Selenium/pharmacology ; Selenium/therapeutic use ; Solanum lycopersicum ; Testosterone/therapeutic use
Chemical Substances	Androgens ; Antioxidants ; Dihydrotestosterone (08J2K08A3Y) ; Finasteride (57GNO57U7G) ; Receptors, Androgen ; Selenium (H6241UJ22B) ; Testosterone (3XMK78S47O)
Language	English
Publishing date	2023-09-02
Publishing country	Germany
Document type	Journal Article
ZDB-ID	380333-8
ISSN	1433-8726 ; 0724-4983
ISSN (online)	1433-8726
ISSN	0724-4983
DOI	10.1007/s00345-023-04558-x
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Article ; Online: Selenium in combination with a tomato lipid extract as a therapy for benign prostatic hyperplasia and its alterations in rats with induced BPH.

Arias-Chávez, David Julian / Mailloux-Salinas, Patrick / Ledesma Aparicio, Jessica / Campos-Pérez, Elihu / Medina-Campos, Omar Noel / Pedraza-Chaverri, José / Bravo, Guadalupe

Journal of cellular and molecular medicine

2023 Volume 27, Issue 20, Page(s) 3147–3156

Abstract: Benign prostatic hyperplasia (BPH) is the most common adenoma in old men. Tomatoes are a rich source of bioactive compounds that, as well as selenium (Se), possess antioxidant and antiproliferative activity. The aim was to evaluate the therapeutic effect ...

Abstract	Benign prostatic hyperplasia (BPH) is the most common adenoma in old men. Tomatoes are a rich source of bioactive compounds that, as well as selenium (Se), possess antioxidant and antiproliferative activity. The aim was to evaluate the therapeutic effect of Se in combination with a tomato extract in aged rats with BPH. Aged male Wistar rats were divided in the following groups (n = 10 rats/group): Control (C), BPH, BPH + Finasteride (BPH + F), BPH + Tomato Lipidic Extract (BPH + E), BPH + Selenium (BPH + S) and BPH plus E plus S (BPH + E + S). After 4 weeks of treatment, prostate weight, diuresis, antioxidants enzymes, prooxidants and inflammatory markers, growth factors and androgens were determined. BPH + E + S reduced prostate weight by 59.29% and inhibited growth by 99.35% compared to BPH + F which only decreased weight and inhibited growth by 15.31% and 57.54%, respectively. Prooxidant markers were higher with BPH + F (49.4% higher vs. BPH), but BPH + E + S decreased these markers (94.27% vs. BPH) and increased antioxidant activity. Finally, diuresis was higher with the BPH + E + S combination and markers of inflammation and growth factors were significantly lower with respect to BPH + F. Our findings provide a beneficial and protective therapeutic option of E + S directed against androgens, oxidative stress and inflammation that regulates cell proliferation in the prostate gland.
Language	English
Publishing date	2023-09-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2074559-X
ISSN	1582-4934 ; 1582-4934 ; 1582-1838
ISSN (online)	1582-4934
ISSN	1582-4934 ; 1582-1838
DOI	10.1111/jcmm.17903
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Zs.A 5752: Show issues

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Article ; Online: Chronic consumption of imbalance diets high in sucrose or fat induces abdominal obesity with different pattern of metabolic disturbances and lost in Langerhans cells population.

Márquez Álvarez, Corazón de María / Gómez-Crisóstomo, Nancy P / De la Cruz-Hernández, Erick N / El-Hafidi, Mohammed / Pedraza-Chaverri, José / Medina-Campos, Omar Noel / Martínez-Abundis, Eduardo

Life sciences

2023 Volume 336, Page(s) 122305

Abstract: Aim: Obesity is a worldwide health issue, associated with development of type 2 Diabetes Mellitus. The aim of this study is to analyze the effect of consumption of two hypercaloric diets on metabolic disturbance and beta cells damage.: Main methods: ... ...

Abstract	Aim: Obesity is a worldwide health issue, associated with development of type 2 Diabetes Mellitus. The aim of this study is to analyze the effect of consumption of two hypercaloric diets on metabolic disturbance and beta cells damage. Main methods: Male Wistar rats were subjected to twelve months consumption of three diets: a Control balanced diet (CTD, carbohydrates 58 %, proteins 29 %, lipids 13 %) and two hypercaloric diets, high in sucrose (HSD, carbohydrates 68 %, proteins 22 %, lipids 10 %) or high in fat (HFD, carbohydrates 31 %, proteins 14 %, lipids 55 %). Serum levels of glucose, triglycerides and free fatty acids were measured after zoometric parameters determination. Antioxidant enzymes activity and oxidative stress-marker were measured in pancreas tissue among histological analysis of Langerhans islets. Key findings: Although diets were hypercaloric, the amount of food consumed by rats decreased, resulting in an equal caloric consumption. The HSD induced hypertriglyceridemia and hyperglycemia with higher levels in free fatty acids (FFA, lipotoxicity); whereas HFD did not increased neither the triglycerides nor FFA, nevertheless the loss of islets' cell was larger. Both diets induced obesity with hyperglycemia and significant reduction in Langerhans islets size. Significance: Our results demonstrate that consumption of HSD induces more significant metabolic disturbances that HFD, although both generated pancreas damage; as well hypercaloric diet consumption is not indispensable to becoming obese; the chronic consumption of unbalanced diets (rich in carbohydrates or lipids) may lead to abdominal obesity with metabolic and functional disturbances, although the total amount of calories are similar.
MeSH term(s)	Male ; Rats ; Animals ; Diabetes Mellitus, Type 2/etiology ; Obesity, Abdominal/etiology ; Sucrose ; Fatty Acids, Nonesterified ; Langerhans Cells/metabolism ; Rats, Wistar ; Blood Glucose/metabolism ; Obesity/metabolism ; Diet ; Triglycerides/metabolism ; Hyperglycemia ; Diet, High-Fat/adverse effects
Chemical Substances	Sucrose (57-50-1) ; Fatty Acids, Nonesterified ; Blood Glucose ; Triglycerides
Language	English
Publishing date	2023-11-27
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	3378-9
ISSN	1879-0631 ; 0024-3205
ISSN (online)	1879-0631
ISSN	0024-3205
DOI	10.1016/j.lfs.2023.122305
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Article ; Online: α-Mangostin induces oxidative damage, mitochondrial dysfunction, and apoptosis in a triple-negative breast cancer model.

Cruz-Gregorio, Alfredo / Aranda-Rivera, Ana Karina / Aparicio-Trejo, Omar Emiliano / Medina-Campos, Omar Noel / Sciutto, Edda / Fragoso, Gladis / Pedraza-Chaverri, José

Phytotherapy research : PTR

2023 Volume 37, Issue 8, Page(s) 3394–3407

Abstract: Triple-negative breast cancer (TNBC) does not express estrogen receptor, progesterone receptor, and human epidermal growth factor receptor; therefore, TNBC lacks targeted therapy, and chemotherapy is the only available treatment for this illness but ... ...

Abstract	Triple-negative breast cancer (TNBC) does not express estrogen receptor, progesterone receptor, and human epidermal growth factor receptor; therefore, TNBC lacks targeted therapy, and chemotherapy is the only available treatment for this illness but causes side effects. A putative strategy for the treatment of TNBC could be the use of the polyphenols such as α-Mangostin (α-M), which has shown anticancerogenic effects in different cancer models and can modulate the inflammatory and prooxidant state in several pathological models. The redox state, oxidative stress (OS), and oxidative damage are highly related to cancer development and its treatment. Thus, this study aimed to evaluate the effects of α-M on redox state, mitochondrial metabolism, and apoptosis in 4T1 mammary carcinoma cells. We found that α-M decreases both protein levels and enzymatic activity of catalase, and increases reactive oxygen species, oxidized proteins and glutathione disulfide, which demonstrates that α-M induces oxidative damage. We also found that α-M promotes mitochondrial dysfunction by abating basal respiration, the respiration ligated to oxidative phosphorylation (OXPHOS), and the rate control of whole 4T1 cells. Additionally, α-M also decreases the levels of OXPHOS subunits of mitochondrial complexes I, II, III, and adenosine triphosphate synthase, the activity of mitochondrial complex I as well as the levels of peroxisome proliferator-activated receptor-gamma co-activator 1α, showing a mitochondrial mass reduction. Then, oxidative damage and mitochondrial dysfunction induced by α-M induce apoptosis of 4T1 cells, which is evidenced by B cell lymphoma 2 decrease and caspase 3 cleavage. Taken together, our results suggest that α-M induces OS and mitochondrial dysfunction, resulting in 4T1 cell death through apoptotic mechanisms.
MeSH term(s)	Humans ; Triple Negative Breast Neoplasms/drug therapy ; Oxidative Stress ; Reactive Oxygen Species/metabolism ; Apoptosis ; Mitochondria
Chemical Substances	mangostin (U6RIV93RU1) ; Reactive Oxygen Species
Language	English
Publishing date	2023-04-03
Publishing country	England
Document type	Journal Article
ZDB-ID	639136-9
ISSN	1099-1573 ; 0951-418X
ISSN (online)	1099-1573
ISSN	0951-418X
DOI	10.1002/ptr.7812
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Article: Effect of the Age and Body Weight of the Broiler Breeders Male on the Presentation of Oxidative Stress and Its Correlation with the Quality of Testicular Parenchyma and Physiological Antioxidant Levels

Escorcia, Magdalena / Sánchez-Godoy, Félix / Ramos-Vidales, David / Medina-Campos, Omar Noel / Pedraza-Chaverri, José

Veterinary sciences. 2020 May 26, v. 7, no. 2

2020

Abstract: Chicken meat is a food of high nutritional quality; its production requires birds called broilers breeders and looking after all aspects that influence their reproductive capacity. An ongoing controversy exists among researchers related to the weight of ... ...

Abstract	Chicken meat is a food of high nutritional quality; its production requires birds called broilers breeders and looking after all aspects that influence their reproductive capacity. An ongoing controversy exists among researchers related to the weight of the rooster and its fertilization capacity. By histological and biochemical tests, the association between weight and age with oxidant damage, testicular parenchyma and antioxidant capacity was evaluated in Ross 308 roosters. Testicular integrity was assessed by histological analysis, oxidative stress was determined by malondialdehyde content, non-enzymatic antioxidant capacity was determined by oxygen radical absorbance capacity assay and enzymatic antioxidant capacity through glutathione peroxidase, glutathione reductase and glutathione-S-transferase activities. Histological analysis showed vacuolization of the epithelium from the seminiferous tubules. A significant negative association was observed between malondialdehyde and the deterioration of the integrity of the seminiferous epithelium, as well as between age and integrity of the seminiferous epithelium. It became evident that oxidative damage directly affects the quality of testicular parenchyma. Weight and age were not associated with the antioxidant enzymes activities, but with non-enzymatic capacity. The data obtained suggest that weight is not the most important factor that influences the fertility of the rooster.
Keywords	body weight ; chicken meat ; glutathione peroxidase ; glutathione transferase ; glutathione-disulfide reductase ; histology ; malondialdehyde ; nutritive value ; oxidants ; oxidative stress ; oxygen radical absorbance capacity ; reproductive performance ; roosters ; seminiferous epithelium
Language	English
Dates of publication	2020-0526
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2768971-2
ISSN	2306-7381
ISSN	2306-7381
DOI	10.3390/vetsci7020069
Database	NAL-Catalogue (AGRICOLA)

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