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  1. AU="Meeremans, Marguerite"
  2. AU="Chen, Yanguang"
  3. AU="Sakizono, Kenji"
  4. AU="Romero-Daza, Nancy"
  5. AU="Jean-Pierre Thomé"
  6. AU=Narayanan Naveen
  7. AU=Azam Faizul
  8. AU="Özdog˘ru, Asil Ali"
  9. AU="Emami, Hajar"
  10. AU="Cimino, R."
  11. AU="Judith R. Stabel"
  12. AU="Takeuchi, Kazuto"
  13. AU="Mirzaei, Samira"
  14. AU="Carolina Salgado"
  15. AU="Mate, Sebastian"
  16. AU="Hou, Tian-Yang Liu"
  17. AU=Nino Gustavo
  18. AU="Lydon, Myra"
  19. AU="Jain, Nibha"
  20. AU="David A Schwartz"
  21. AU="Swart, Jonathan"
  22. AU="Karol, Agnieszka"
  23. AU="Reilly, Brittni"
  24. AU="Arfaatabar, Maryam"
  25. AU="Kumar Pandey, Anand"

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  1. Artikel: Validation of a color deconvolution method to quantify MSC tri-lineage differentiation across species.

    Heyman, Emma / Meeremans, Marguerite / Devriendt, Bert / Olenic, Maria / Chiers, Koen / De Schauwer, Catharina

    Frontiers in veterinary science

    2022  Band 9, Seite(n) 987045

    Abstract: Mesenchymal stem cells (MSCs) are a promising candidate for both human and veterinary regenerative medicine applications because of their abundance and ability to differentiate into several lineages. Mesenchymal stem cells are however a heterogeneous ... ...

    Abstract Mesenchymal stem cells (MSCs) are a promising candidate for both human and veterinary regenerative medicine applications because of their abundance and ability to differentiate into several lineages. Mesenchymal stem cells are however a heterogeneous cell population and as such, it is imperative that they are unequivocally characterized to acquire reproducible results in clinical trials. Although the tri-lineage differentiation potential of MSCs is reported in most veterinary studies, a qualitative evaluation of representative histological images does not always unambiguously confirm tri-lineage differentiation. Moreover, potential differences in differentiation capacity are not identified. Therefore, quantification of tri-lineage differentiation would greatly enhance proper characterization of MSCs. In this study, a method to quantify the tri-lineage differentiation potential of MSCs is described using digital image analysis, based on the color deconvolution plug-in (ImageJ). Mesenchymal stem cells from three species, i.e., bovine, equine, and porcine, were differentiated toward adipocytes, chondrocytes, and osteocytes. Subsequently, differentiated MSCs were stained with Oil Red O, Alcian Blue, and Alizarin Red S, respectively. Next, a differentiation ratio (DR) was obtained by dividing the area % of the differentiation signal by the area % of the nuclear signal. Although MSCs isolated from all donors in all species were capable of tri-lineage differentiation, differences were demonstrated between donors using this quantitative DR. Our straightforward, simple but robust method represents an elegant approach to determine the degree of MSC tri-lineage differentiation across species. As such, differences in differentiation potential within the heterogeneous MSC population and between different MSC sources can easily be identified, which will support further optimization of regenerative therapies.
    Sprache Englisch
    Erscheinungsdatum 2022-10-13
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2022.987045
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: The Lack of a Representative Tendinopathy Model Hampers Fundamental Mesenchymal Stem Cell Research.

    Meeremans, Marguerite / Van de Walle, Gerlinde R / Van Vlierberghe, Sandra / De Schauwer, Catharina

    Frontiers in cell and developmental biology

    2021  Band 9, Seite(n) 651164

    Abstract: Overuse tendon injuries are a major cause of musculoskeletal morbidity in both human and equine athletes, due to the cumulative degenerative damage. These injuries present significant challenges as the healing process often results in the formation of ... ...

    Abstract Overuse tendon injuries are a major cause of musculoskeletal morbidity in both human and equine athletes, due to the cumulative degenerative damage. These injuries present significant challenges as the healing process often results in the formation of inferior scar tissue. The poor success with conventional therapy supports the need to search for novel treatments to restore functionality and regenerate tissue as close to native tendon as possible. Mesenchymal stem cell (MSC)-based strategies represent promising therapeutic tools for tendon repair in both human and veterinary medicine. The translation of tissue engineering strategies from basic research findings, however, into clinical use has been hampered by the limited understanding of the multifaceted MSC mechanisms of action.
    Sprache Englisch
    Erscheinungsdatum 2021-05-03
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2021.651164
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Equine Tenocyte Seeding on Gelatin Hydrogels Improves Elongated Morphology.

    Meeremans, Marguerite / Van Damme, Lana / De Spiegelaere, Ward / Van Vlierberghe, Sandra / De Schauwer, Catharina

    Polymers

    2021  Band 13, Heft 5

    Abstract: 1) Background: Tendinopathy is a common injury in both human and equine athletes. Representative in vitro models are mandatory to facilitate translation of fundamental research into successful clinical treatments. Natural biomaterials like gelatin ... ...

    Abstract (1) Background: Tendinopathy is a common injury in both human and equine athletes. Representative in vitro models are mandatory to facilitate translation of fundamental research into successful clinical treatments. Natural biomaterials like gelatin provide favorable cell binding characteristics and are easily modifiable. In this study, methacrylated gelatin (gel-MA) and norbornene-functionalized gelatin (gel-NB), crosslinked with 1,4-dithiotreitol (DTT) or thiolated gelatin (gel-SH) were compared. (2) Methods: The physicochemical properties (
    Sprache Englisch
    Erscheinungsdatum 2021-02-28
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym13050747
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: Polymeric reinforcements for cellularized collagen-based vascular wall models: influence of the scaffold architecture on the mechanical and biological properties.

    Pien, Nele / Di Francesco, Dalila / Copes, Francesco / Bartolf-Kopp, Michael / Chausse, Victor / Meeremans, Marguerite / Pegueroles, Marta / Jüngst, Tomasz / De Schauwer, Catharina / Boccafoschi, Francesca / Dubruel, Peter / Van Vlierberghe, Sandra / Mantovani, Diego

    Frontiers in bioengineering and biotechnology

    2023  Band 11, Seite(n) 1285565

    Abstract: A previously developed cellularized collagen-based vascular wall model showed promising results in mimicking the biological properties of a native vessel but lacked appropriate mechanical properties. In this work, we aim to improve this collagen-based ... ...

    Abstract A previously developed cellularized collagen-based vascular wall model showed promising results in mimicking the biological properties of a native vessel but lacked appropriate mechanical properties. In this work, we aim to improve this collagen-based model by reinforcing it using a tubular polymeric (reinforcement) scaffold. The polymeric reinforcements were fabricated exploiting commercial poly (ε-caprolactone) (PCL), a polymer already used to fabricate other FDA-approved and commercially available devices serving medical applications, through 1) solution electrospinning (SES), 2) 3D printing (3DP) and 3) melt electrowriting (MEW). The non-reinforced cellularized collagen-based model was used as a reference (COL). The effect of the scaffold's architecture on the resulting mechanical and biological properties of the reinforced collagen-based model were evaluated. SEM imaging showed the differences in scaffolds' architecture (fiber alignment, fiber diameter and pore size) at both the micro- and the macrolevel. The polymeric scaffold led to significantly improved mechanical properties for the reinforced collagen-based model (initial elastic moduli of 382.05 ± 132.01 kPa, 100.59 ± 31.15 kPa and 245.78 ± 33.54 kPa, respectively for SES, 3DP and MEW at day 7 of maturation) compared to the non-reinforced collagen-based model (16.63 ± 5.69 kPa). Moreover, on day 7, the developed collagen gels showed stresses (for strains between 20% and 55%) in the range of [5-15] kPa for COL, [80-350] kPa for SES, [20-70] kPa for 3DP and [100-190] kPa for MEW. In addition to the effect on the resulting mechanical properties, the polymeric tubes' architecture influenced cell behavior, in terms of proliferation and attachment, along with collagen gel compaction and extracellular matrix protein expression. The MEW reinforcement resulted in a collagen gel compaction similar to the COL reference, whereas 3DP and SES led to thinner and longer collagen gels. Overall, it can be concluded that 1) the selected processing technique influences the scaffolds' architecture, which in turn influences the resulting mechanical and biological properties, and 2) the incorporation of a polymeric reinforcement leads to mechanical properties closely matching those of native arteries.
    Sprache Englisch
    Erscheinungsdatum 2023-11-16
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2023.1285565
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Cell Guiding Multicomponent Nanoyarn Tendon Scaffolds with Tunable Morphology and Flexibility.

    Schynkel, Lucas / Meeremans, Marguerite / Meyer, Anna A / Schoolaert, Ella / Geltmeyer, Jozefien / Omidinia-Anarkoli, Abdolrahman / Van Vlierberghe, Sandra / Daelemans, Lode / De Laporte, Laura / De Schauwer, Catharina / Hoogenboom, Richard / De Clerck, Karen

    ACS applied materials & interfaces

    2023  Band 15, Heft 36, Seite(n) 42241–42250

    Abstract: Nanofibrous scaffolds are widely investigated for tendon tissue engineering due to their porous structure, high flexibility, and the ability to guide cells in a preferred direction. Previous research has shown that providing a microenvironment similar to ...

    Abstract Nanofibrous scaffolds are widely investigated for tendon tissue engineering due to their porous structure, high flexibility, and the ability to guide cells in a preferred direction. Previous research has shown that providing a microenvironment similar to in vivo settings improves tissue regeneration. Therefore, in this work, ingenious multicomponent nanoyarn scaffolds that mimic the fibrillar and tubular structures of tendons are developed for the first time through electrospinning and bundling nanoyarns followed by electrospinning of a nanofibrous shell around the bundle. Multicomponent nanoyarn scaffolds out of poly(ε-caprolactone) with varying porosity, density, and diameter were successfully produced by coelectrospinning with water-soluble poly(2-ethyl-2-oxazoline) as a sacrificial component. The diameter and fiber orientation of the nanoyarns were successfully tuned based on parameter-morphology models obtained by the design of experiments. Cyclic bending tests were performed, indicating that the flexibility of the multicomponent nanoyarn scaffolds depends on the morphology and can be tuned through controlling the number of nanoyarns in the bundle and the porosity. Indirect and direct cell culture tests using mouse and equine tendon cells revealed excellent cytocompatibility of the nanofibrous products and demonstrated the potential of the nanoyarns to guide the growing cells along the nanofiber direction, which is crucial for tendon tissue engineering.
    Mesh-Begriff(e) Animals ; Horses ; Mice ; Cell Culture Techniques ; Cytoskeleton ; Nanofibers ; Poly A ; Tendons
    Chemische Substanzen Poly A (24937-83-5)
    Sprache Englisch
    Erscheinungsdatum 2023-08-31
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 1944-8252
    ISSN (online) 1944-8252
    DOI 10.1021/acsami.3c08241
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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