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Article ; Online: Structural Study of the Exocyst Subunit Human Sec6.

Guo, Jiatian / Mei, Kunrong

Studies in health technology and informatics

2023 Volume 308, Page(s) 351–358

Abstract: In eukaryotic cells, vesicular transport plays a crucial role in the docking and fusion of secretory vesicles with their respective target membranes. This intricate process is dependent on a complex network of multiple molecules. One of the important ... ...

Abstract	In eukaryotic cells, vesicular transport plays a crucial role in the docking and fusion of secretory vesicles with their respective target membranes. This intricate process is dependent on a complex network of multiple molecules. One of the important processes is tethering. The exocyst complex facilitates the tethering of secretory vesicles to the plasma membrane during exocytosis. The Sec6 subunit in yeast interacts with other exocyst subunits and may regulate SNARE assembly, which is crucial for understanding the assembly mechanism of exocyst and its interaction with SNARE. In this study, we designed two truncated forms of HuSec6, HuSec6 121-734 and HuSec6 121-745, based on results of bioinformatics analysis. We expressed and purified the proteins in E. coli, obtaining a protein purity of over 95% and protein crystals. X-ray diffraction results showed a resolution of approximately 9 Å for the crystals, providing a solid foundation for the crystal structure analysis of HuSec6.
MeSH term(s)	Humans ; Escherichia coli/metabolism ; Exocytosis/physiology ; Saccharomyces cerevisiae/metabolism ; SNARE Proteins/metabolism ; Vesicular Transport Proteins/chemistry ; Vesicular Transport Proteins/metabolism
Chemical Substances	SNARE Proteins ; Vesicular Transport Proteins ; AT1G71820 protein, Arabidopsis
Language	English
Publishing date	2023-11-25
Publishing country	Netherlands
Document type	Journal Article
ISSN	1879-8365
ISSN (online)	1879-8365
DOI	10.3233/SHTI230859
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Article ; Online: Expression and Purification of SMGL-1.

Wang, Mengya / Mei, Kunrong

Studies in health technology and informatics

2023 Volume 308, Page(s) 68–75

Abstract: Recombinant protein expression is a crucial technique in biology, with E. coli being the most widely used expression system. However, due to growth pressure, the expression of large molecular weight proteins in E. coli has remained a challenging task. ... ...

Abstract	Recombinant protein expression is a crucial technique in biology, with E. coli being the most widely used expression system. However, due to growth pressure, the expression of large molecular weight proteins in E. coli has remained a challenging task. SMGL-1, a newly discovered protein in C. elegans with Rabin 8 function, plays an important role in biology. To better understand the function of SMGL-1, we first predicted and analyzed its protein structure and properties using artificial intelligence. We then conducted studies on its expression and purification. Through optimization of IPTG concentration and expression strains, we successfully expressed SMGL-1 in E. coli, providing guidance for the expression of large proteins in E. coli. Furthermore, we explored the purification of SMGL-1 using GST affinity chromatography, Nickel affinity chromatography, and ammonium sulfate precipitation methods, laying the foundation for future purification work on SMGL-1.
MeSH term(s)	Animals ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Artificial Intelligence ; Caenorhabditis elegans
Language	English
Publishing date	2023-11-26
Publishing country	Netherlands
Document type	Journal Article
ISSN	1879-8365
ISSN (online)	1879-8365
DOI	10.3233/SHTI230826
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Article ; Online: Adaptive nonsingular terminal sliding mode controller for PMSM drive system using modified extended state observer.

Shi, Ying / Mei, Keqi

Mathematical biosciences and engineering : MBE

2023 Volume 20, Issue 10, Page(s) 18774–18791

Abstract: In this study, a novel adaptive nonsingular terminal sliding mode (ANTSM) control frame combined with a modified extended state observer (MESO) is presented to enhance the anti-interference performance of the permanent magnet synchronous motor (PMSM) ... ...

Abstract	In this study, a novel adaptive nonsingular terminal sliding mode (ANTSM) control frame combined with a modified extended state observer (MESO) is presented to enhance the anti-interference performance of the permanent magnet synchronous motor (PMSM) system. In the face of time-varying disturbances with unknown upper bounds, traditional nonsingular terminal sliding mode (NTSM) controllers typically utilize the large control gain to counteract the total disturbance, which will cause unsatisfactory control performances. To address this tough issue, an ANTSM control technique was constructed for the PMSM system by tuning the control gain automatically without overestimation. On this basis, the MESO was adopted to estimate the unknown total disturbance, whose estimation was offset to the ANTSM control input. By applying finite-time techniques, the estimation error will finite-time converge to zero. The proposed MESO has a more rapid estimation speed than the traditional extended state observer (ESO). Finally, the validity of the ANTSM + ESO composite control algorithm is confirmed by comprehensive experiments.
Language	English
Publishing date	2023-12-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	2265126-3
ISSN	1551-0018 ; 1551-0018
ISSN (online)	1551-0018
ISSN	1551-0018
DOI	10.3934/mbe.2023832
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Article ; Online: Modeling the Cryo-EM Structure of the Exocyst Complex.

Mei, Kunrong / Guo, Wei

Methods in molecular biology (Clifton, N.J.)

2022 Volume 2557, Page(s) 247–262

Abstract: Multi-subunit tethering complexes (MTCs) are a family of evolutionarily conserved large protein complexes that function to tether intracellular vesicles from the donor compartments to the membrane of receptor compartments. The exocyst complex is an ... ...

Abstract	Multi-subunit tethering complexes (MTCs) are a family of evolutionarily conserved large protein complexes that function to tether intracellular vesicles from the donor compartments to the membrane of receptor compartments. The exocyst complex is an octameric MTC that tethers the post-Golgi secretory vesicles to the plasma membrane. To learn the function and regulation of the exocyst complex, it is crucial to understand the structure of the complex. We have solved the cryo-EM structure of the exocyst complex at 4.4 Angstrom (Å) resolution and detected the spatial relationship between the eight subunits using chemical cross-linking mass spectrometry. Here, we describe the method of modeling and validating the cryo-EM structure of the exocyst complex. This method could provide a guide for modeling of other protein complexes of which the structures are solved at medium to near-atomic resolution.
MeSH term(s)	Cryoelectron Microscopy ; Cytoplasm ; Secretory Vesicles/metabolism ; Cell Membrane/metabolism ; Golgi Apparatus/metabolism ; Exocytosis
Language	English
Publishing date	2022-12-11
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-2639-9_16
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Article ; Online: NiFeP nanosheets for efficient and durable hydrazine-assisted electrolytic hydrogen production.

Hou, Jinxing / Mei, Kaifeng / Jiang, Tongtong / Yu, Xinxin / Wu, Mingzai

Dalton transactions (Cambridge, England : 2003)

2024 Volume 53, Issue 10, Page(s) 4574–4579

Abstract: Hydrazine-assisted electrochemical water splitting is an important avenue toward low cost and sustainable hydrogen production, which can significantly reduce the voltage of electrochemical water splitting. Herein, we took a simple approach to fabricate ... ...

Abstract	Hydrazine-assisted electrochemical water splitting is an important avenue toward low cost and sustainable hydrogen production, which can significantly reduce the voltage of electrochemical water splitting. Herein, we took a simple approach to fabricate NiFeP nanosheet arrays on nickel foam (NiFeP/NF), which exhibit superior electrocatalytic activity for the hydrogen evolution reaction (HER) and the hydrazine oxidation reaction (HzOR). Our investigations revealed that the excellent electrocatalytic activity of NiFeP/NF mainly arises from the bimetallic synergistic effect, abundant electrocatalytically active sites facilitated by the porous nanosheet morphology, high intrinsic conductivity of NiFeP/NF and strong NiFeP-NF adhesion. We assembled a hydrazine-boosted electrochemical water splitting cell using NiFeP/NF as a bifunctional catalyst for both electrodes, and the overall hydrazine splitting (OHzS) exhibits a considerably low overpotential (100 mV at 10 mA cm
Language	English
Publishing date	2024-03-05
Publishing country	England
Document type	Journal Article
ZDB-ID	1472887-4
ISSN	1477-9234 ; 1364-5447 ; 0300-9246 ; 1477-9226
ISSN (online)	1477-9234 ; 1364-5447
ISSN	0300-9246 ; 1477-9226
DOI	10.1039/d3dt04373h
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Article ; Online: Effect of Negative Online Reviews and Physician Responses on Health Consumers' Choice: Experimental Study.

Han, Xi / Lin, Yongxi / Han, Wenting / Liao, Ke / Mei, Kefu

Journal of medical Internet research

2024 Volume 26, Page(s) e46713

Abstract: Background: The COVID-19 pandemic has highlighted the importance of online medical services. Although some researchers have investigated how numerical ratings affect consumer choice, limited studies have focused on the effect of negative reviews that ... ...

Abstract	Background: The COVID-19 pandemic has highlighted the importance of online medical services. Although some researchers have investigated how numerical ratings affect consumer choice, limited studies have focused on the effect of negative reviews that most concern physicians. Objective: This study aimed to investigate how negative review features, including proportion (low/high), claim type (evaluative/factual), and physician response (absence/presence), influence consumers' physician evaluation process under conditions in which a physician's overall rating is high. Methods: Using a 2×2×2 between-subject decision-controlled experiment, this study examined participants' judgment on physicians with different textual reviews. Collected data were analyzed using the t test and partial least squares-structural equation modeling. Results: Negative reviews decreased consumers' physician selection intention. The negative review proportion (β=-0.371, P<.001) and claim type (β=-0.343, P<.001) had a greater effect on consumers' physician selection intention compared to the physician response (β=0.194, P<.001). A high negative review proportion, factual negative reviews, and the absence of a physician response significantly reduced consumers' physician selection intention compared to their counterparts. Consumers' locus attributions on the negative reviews affected their evaluation process. Physician attribution mediated the effects of review proportion (β=-0.150, P<.001), review claim type (β=-0.068, P=.01), and physician response (β=0.167, P<.001) on consumer choice. Reviewer attribution also mediated the effects of review proportion (β=-0.071, P<.001), review claim type (β=-0.025, P=.01), and physician response (β=0.096, P<.001) on consumer choice. The moderating effects of the physician response on the relationship between review proportion and physician attribution (β=-0.185, P<.001), review proportion and reviewer attribution (β=-0.110, P<.001), claim type and physician attribution (β=-0.123, P=.003), and claim type and reviewer attribution (β=-0.074, P=.04) were all significant. Conclusions: Negative review features and the physician response significantly influence consumer choice through the causal attribution to physicians and reviewers. Physician attribution has a greater effect on consumers' physician selection intention than reviewer attribution does. The presence of a physician response decreases the influence of negative reviews through direct and moderating effects. We propose some practical implications for physicians, health care providers, and online medical service platforms.
MeSH term(s)	Humans ; Pandemics ; Physicians ; Health Personnel ; COVID-19 ; Data Collection
Language	English
Publishing date	2024-03-12
Publishing country	Canada
Document type	Journal Article
ZDB-ID	2028830-X
ISSN	1438-8871 ; 1438-8871
ISSN (online)	1438-8871
ISSN	1438-8871
DOI	10.2196/46713
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Article ; Online: The double-edged effect of social mobility belief on socioeconomically disadvantaged adolescents' health: The mediating role of intentional self-regulation.

Zuo, Chenyi / Ren, Yi / Ming, Hua / Mei, Kehan / Huang, Silin

Health psychology : official journal of the Division of Health Psychology, American Psychological Association

2024

Abstract: Purpose: This study aimed to examine the double-edged effect of social mobility belief on socioeconomically disadvantaged adolescents' mental and physical health and further explore whether intentional self-regulation is the common psychological ... ...

Abstract	Purpose: This study aimed to examine the double-edged effect of social mobility belief on socioeconomically disadvantaged adolescents' mental and physical health and further explore whether intentional self-regulation is the common psychological mechanism of social mobility belief affecting physical and mental health. Method: A total of 469 adolescents ( Results: Social mobility belief was positively correlated with adolescents' life satisfaction and self-esteem but negatively correlated with depression. Intentional self-regulation mediated the relationships between social mobility belief and mental health. In addition, the results showed that intentional self-regulation mediated the relationship between social mobility belief and adolescents' physical health. Conclusions: Social mobility belief may be a "skin-deep" resilience resource positively related to mental health but negatively correlated with physical health through intentional self-regulation among socioeconomically disadvantaged adolescents. (PsycInfo Database Record (c) 2024 APA, all rights reserved).
Language	English
Publishing date	2024-04-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	226369-5
ISSN	1930-7810 ; 0278-6133
ISSN (online)	1930-7810
ISSN	0278-6133
DOI	10.1037/hea0001375
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Article ; Online: Dynamics and geochemical responses of dissolved metals (Mn and Cu) in a subtropical estuary, China.

Mei, Kang / Shi, Mengqiu / Chen, Nengwang / Wang, Deli

Environmental science and pollution research international

2023 Volume 31, Issue 4, Page(s) 6082–6093

Abstract: The research delved into the occurrence and dynamics of dissolved metals, specifically manganese (Mn) and copper (Cu), within the Jiulong River Estuary, South China, a medium-sized subtropical estuary. Our findings unveiled a nuanced seasonal and spatial ...

Abstract	The research delved into the occurrence and dynamics of dissolved metals, specifically manganese (Mn) and copper (Cu), within the Jiulong River Estuary, South China, a medium-sized subtropical estuary. Our findings unveiled a nuanced seasonal and spatial variability of dissolved metals throughout the entire estuarine system. Notably, dissolved Mn concentrations peaked (~ 3.5 μM) in the upper estuary, diminishing sharply along the salinity gradient, with a modest rise in the middle estuary and outer Xiamen Bay. In the upper estuary, heightened concentrations of dissolved Mn occurred in spring due to augmented terrestrial particle inputs, followed by suboxically reductive releases; conversely, concentrations were low in summer, attributed to dilution from increased freshwater discharges and particle scavenging. In contrast, dissolved Cu exhibited differently, with elevated concentrations (29.2-37.5 nM) in the upper and middle estuaries, driven by reductive dissolution of Mn particles and chloride-induced ion exchanges, respectively. Concurrently, heightened inputs of nutrients and metals correlated with elevated phytoplankton productivity (indicated by chlorophyll a) in the upper and outer estuary regions. Our analysis underscored the sensitivity of dissolved metals to environmental parameters, including temperature, pH, and dissolved oxygen. The integration of compiled historical data underscored the dynamic nature of dissolved metals, particularly Cu, in response to geochemical processes.The elevated ion levels indicated intensified ion releases from particles and sediments, attributable to increased anthropogenic perturbation and climatic changes (e. g. ocean warming).
MeSH term(s)	Manganese/analysis ; Metals, Heavy/analysis ; Estuaries ; Chlorophyll A/analysis ; Environmental Monitoring ; Water Pollutants, Chemical/analysis ; Rivers ; China ; Geologic Sediments
Chemical Substances	Manganese (42Z2K6ZL8P) ; Metals, Heavy ; Chlorophyll A (YF5Q9EJC8Y) ; Water Pollutants, Chemical
Language	English
Publishing date	2023-12-26
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-023-31387-7
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Article ; Online: Comparing the clinical efficacy of home-made Chinese herbal lotion with conventional western medicine in the treatment of chronic rhinosinusitis.

Mei, Kunyu / Pan, Weiyu / Wang, Hui / Liu, Jingmin

Panminerva medica

2023

Language	English
Publishing date	2023-03-15
Publishing country	Italy
Document type	Journal Article
ZDB-ID	123572-2
ISSN	1827-1898 ; 0031-0808
ISSN (online)	1827-1898
ISSN	0031-0808
DOI	10.23736/S0031-0808.23.04844-9
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Article ; Online: A Generalized Supertwisting Algorithm.

Mei, Keqi / Ding, Shihong / Yu, Xinghuo

IEEE transactions on cybernetics

2023 Volume 53, Issue 6, Page(s) 3951–3960

Abstract: The work proposes a generalized supertwisting algorithm (GSTA) and its constructive design strategy. In contrast with the conventional STA, the most remarkable characteristic of the proposed method is that the discontinuous term in the conventional STA ... ...

Abstract	The work proposes a generalized supertwisting algorithm (GSTA) and its constructive design strategy. In contrast with the conventional STA, the most remarkable characteristic of the proposed method is that the discontinuous term in the conventional STA is replaced with a fractional power term, which can fundamentally improve the performance of the conventional STA. It is shown that if the fractional power in the nonsmooth term becomes -1/2, the GSTA will reduce to the conventional STA. Under the GSTA, it will be rigorously verified by taking advantage of strict Lyapunov analysis that the sliding variables can finite-time converge to an arbitrarily small region in a neighborhood of the origin by tuning the gains and the fractional power. Finally, simulation studies are provided to demonstrate the superiority of the theoretically obtained results.
Language	English
Publishing date	2023-05-17
Publishing country	United States
Document type	Journal Article
ISSN	2168-2275
ISSN (online)	2168-2275
DOI	10.1109/TCYB.2022.3188877
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