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  1. Article: Synchronously pumped Raman laser for simultaneous degenerate and nondegenerate two-photon microscopy.

    Buttolph, Michael L / Mejooli, Menansili A / Sidorenko, Pavel / Eom, Chi-Yong / Schaffer, Chris B / Wise, Frank W

    Biomedical optics express

    2021  Volume 12, Issue 4, Page(s) 2496–2507

    Abstract: Two-photon fluorescence microscopy is a nonlinear imaging modality frequently used in deep-tissue imaging applications. A tunable-wavelength multicolor short-pulse source is usually required to excite fluorophores with a wide range of excitation ... ...

    Abstract Two-photon fluorescence microscopy is a nonlinear imaging modality frequently used in deep-tissue imaging applications. A tunable-wavelength multicolor short-pulse source is usually required to excite fluorophores with a wide range of excitation wavelengths. This need is most typically met by solid-state lasers, which are bulky, expensive, and complicated systems. Here, we demonstrate a compact, robust fiber system that generates naturally synchronized femtosecond pulses at 1050 nm and 1200 nm by using a combination of gain-managed and Raman amplification. We image the brain of a mouse and view the blood vessels, neurons, and other cell-like structures using simultaneous degenerate and nondegenerate excitation.
    Language English
    Publishing date 2021-03-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2572216-5
    ISSN 2156-7085
    ISSN 2156-7085
    DOI 10.1364/BOE.421647
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Hyperspectral multiphoton microscopy for

    Bares, Amanda J / Mejooli, Menansili A / Pender, Mitchell A / Leddon, Scott A / Tilley, Steven / Lin, Karen / Dong, Jingyuan / Kim, Minsoo / Fowell, Deborah J / Nishimura, Nozomi / Schaffer, Chris B

    Optica

    2021  Volume 7, Issue 11, Page(s) 1587–1601

    Abstract: The insensitivity of multiphoton microscopy to optical scattering enables high-resolution, high-contrast imaging deep into tissue, including in live animals. Scattering does, however, severely limit the use of spectral dispersion techniques to improve ... ...

    Abstract The insensitivity of multiphoton microscopy to optical scattering enables high-resolution, high-contrast imaging deep into tissue, including in live animals. Scattering does, however, severely limit the use of spectral dispersion techniques to improve spectral resolution. In practice, this limited spectral resolution together with the need for multiple excitation wavelengths to excite different fluorophores limits multiphoton microscopy to imaging a few, spectrally-distinct fluorescent labels at a time, restricting the complexity of biological processes that can be studied. Here, we demonstrate a hyperspectral multiphoton microscope that utilizes three different wavelength excitation sources together with multiplexed fluorescence emission detection using angle-tuned bandpass filters. This microscope maintains scattering insensitivity, while providing high enough spectral resolution on the emitted fluorescence and capitalizing on the wavelength-dependent nonlinear excitation of fluorescent dyes to enable clean separation of multiple, spectrally overlapping labels,
    Language English
    Publishing date 2021-04-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2779175-0
    ISSN 2334-2536
    ISSN 2334-2536
    DOI 10.1364/optica.389982
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: An intravital window to image the colon in real time.

    Rakhilin, Nikolai / Garrett, Aliesha / Eom, Chi-Yong / Chavez, Katherine Ramos / Small, David M / Daniel, Andrea R / Kaelberer, Melanie M / Mejooli, Menansili A / Huang, Qiang / Ding, Shengli / Kirsch, David G / Bohórquez, Diego V / Nishimura, Nozomi / Barth, Bradley B / Shen, Xiling

    Nature communications

    2019  Volume 10, Issue 1, Page(s) 5647

    Abstract: Intravital microscopy is a powerful technique to observe dynamic processes with single-cell resolution in live animals. No intravital window has been developed for imaging the colon due to its anatomic location and motility, although the colon is a key ... ...

    Abstract Intravital microscopy is a powerful technique to observe dynamic processes with single-cell resolution in live animals. No intravital window has been developed for imaging the colon due to its anatomic location and motility, although the colon is a key organ where the majority of microbiota reside and common diseases such as inflammatory bowel disease, functional gastrointestinal disorders, and colon cancer occur. Here we describe an intravital murine colonic window with a stabilizing ferromagnetic scaffold for chronic imaging, minimizing motion artifacts while maximizing long-term survival by preventing colonic obstruction. Using this setup, we image fluorescently-labeled stem cells, bacteria, and immune cells in live animal colons. Furthermore, we image nerve activity via calcium imaging in real time to demonstrate that electrical sacral nerve stimulation can activate colonic enteric neurons. The simple implantable apparatus enables visualization of live processes in the colon, which will open the window to a broad range of studies.
    MeSH term(s) Animals ; Cell Movement ; Colon/diagnostic imaging ; Colon/microbiology ; Fluorescent Dyes/chemistry ; In Vitro Techniques ; Intravital Microscopy/methods ; Mice ; Mice, Inbred C57BL ; Optical Imaging/methods ; Stem Cells/chemistry ; Stem Cells/cytology
    Chemical Substances Fluorescent Dyes
    Language English
    Publishing date 2019-12-11
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-019-13699-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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