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  1. Article ; Online: Genome-scale and pathway engineering for the sustainable aviation fuel precursor isoprenol production in Pseudomonas putida.

    Banerjee, Deepanwita / Yunus, Ian S / Wang, Xi / Kim, Jinho / Srinivasan, Aparajitha / Menchavez, Russel / Chen, Yan / Gin, Jennifer W / Petzold, Christopher J / Martin, Hector Garcia / Magnuson, Jon K / Adams, Paul D / Simmons, Blake A / Mukhopadhyay, Aindrila / Kim, Joonhoon / Lee, Taek Soon

    Metabolic engineering

    2024  Volume 82, Page(s) 157–170

    Abstract: Sustainable aviation fuel (SAF) will significantly impact global warming in the aviation sector, and important SAF targets are emerging. Isoprenol is a precursor for a promising SAF compound DMCO (1,4-dimethylcyclooctane) and has been produced in several ...

    Abstract Sustainable aviation fuel (SAF) will significantly impact global warming in the aviation sector, and important SAF targets are emerging. Isoprenol is a precursor for a promising SAF compound DMCO (1,4-dimethylcyclooctane) and has been produced in several engineered microorganisms. Recently, Pseudomonas putida has gained interest as a future host for isoprenol bioproduction as it can utilize carbon sources from inexpensive plant biomass. Here, we engineer metabolically versatile host P. putida for isoprenol production. We employ two computational modeling approaches (Bilevel optimization and Constrained Minimal Cut Sets) to predict gene knockout targets and optimize the "IPP-bypass" pathway in P. putida to maximize isoprenol production. Altogether, the highest isoprenol production titer from P. putida was achieved at 3.5 g/L under fed-batch conditions. This combination of computational modeling and strain engineering on P. putida for an advanced biofuels production has vital significance in enabling a bioproduction process that can use renewable carbon streams.
    MeSH term(s) Pseudomonas putida/genetics ; Pseudomonas putida/metabolism ; Carbon/metabolism ; Metabolic Engineering
    Chemical Substances Carbon (7440-44-0)
    Language English
    Publishing date 2024-02-16
    Publishing country Belgium
    Document type Journal Article
    ZDB-ID 1470383-x
    ISSN 1096-7184 ; 1096-7176
    ISSN (online) 1096-7184
    ISSN 1096-7176
    DOI 10.1016/j.ymben.2024.02.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6288: Show issues Location:
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  2. Article ; Online: Ultrasound-enhanced recovery of butanol/ABE by pervaporation.

    Menchavez, Russel Navarro / Ha, Sung Ho

    Applied biochemistry and biotechnology

    2013  Volume 171, Issue 5, Page(s) 1159–1169

    Abstract: The search for renewable sources of energy has led to renewed interests on the biochemical route for the production of butanol. Butanol production suffers from several drawbacks, mainly caused by butanol inhibition to the butanol-producing microorganism ... ...

    Abstract The search for renewable sources of energy has led to renewed interests on the biochemical route for the production of butanol. Butanol production suffers from several drawbacks, mainly caused by butanol inhibition to the butanol-producing microorganism which makes it economically uncompetitive against the chemical process. One possible solution proposed is the in situ recovery of acetone-butanol-ethanol (ABE). Among the in situ recovery options, membrane processes like pervaporation have a great potential. Thus, the effects of temperature, feed concentration, and ultrasound irradiation on permeate concentration and permeation flux for the recovery of butanol/ABE by pervaporation from aqueous solutions were investigated in this study. In the butanol-water system, permeate butanol concentration as well as flux increased with an increase in temperature and butanol feed concentration. When pervaporation studies with ABE-water mixture were carried out at 60 °C for 2, 4, 8, 16, and 24 h, pervaporation profile revealed an optimal permeate concentration as well as permeation flux. Applications of ultrasound irradiation on pervaporation improved permeate concentration by about 23 g/L for both butanol and ABE. Ultrasound irradiation also improved butanol and ABE mass permeation flux by about 13 and 11 %, respectively.
    MeSH term(s) Acetone/chemistry ; Acetone/isolation & purification ; Biotechnology/methods ; Butanols/chemistry ; Butanols/isolation & purification ; Ethanol/chemistry ; Ethanol/isolation & purification ; Phase Transition ; Temperature
    Chemical Substances Butanols ; Acetone (1364PS73AF) ; Ethanol (3K9958V90M)
    Language English
    Publishing date 2013-11
    Publishing country United States
    Document type Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 392344-7
    ISSN 1559-0291 ; 0273-2289
    ISSN (online) 1559-0291
    ISSN 0273-2289
    DOI 10.1007/s12010-013-0196-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3053: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  3. Article: Ultrasound-Enhanced Recovery of Butanol/ABE by Pervaporation

    Menchavez, Russel Navarro / Ha, Sung Ho

    Applied biochemistry and biotechnology. 2013 Nov., v. 171, no. 5

    2013  

    Abstract: The search for renewable sources of energy has led to renewed interests on the biochemical route for the production of butanol. Butanol production suffers from several drawbacks, mainly caused by butanol inhibition to the butanol-producing microorganism ... ...

    Abstract The search for renewable sources of energy has led to renewed interests on the biochemical route for the production of butanol. Butanol production suffers from several drawbacks, mainly caused by butanol inhibition to the butanol-producing microorganism which makes it economically uncompetitive against the chemical process. One possible solution proposed is the in situ recovery of acetone–butanol–ethanol (ABE). Among the in situ recovery options, membrane processes like pervaporation have a great potential. Thus, the effects of temperature, feed concentration, and ultrasound irradiation on permeate concentration and permeation flux for the recovery of butanol/ABE by pervaporation from aqueous solutions were investigated in this study. In the butanol–water system, permeate butanol concentration as well as flux increased with an increase in temperature and butanol feed concentration. When pervaporation studies with ABE–water mixture were carried out at 60� °C for 2, 4, 8, 16, and 24� h, pervaporation profile revealed an optimal permeate concentration as well as permeation flux. Applications of ultrasound irradiation on pervaporation improved permeate concentration by about 23� g/L for both butanol and ABE. Ultrasound irradiation also improved butanol and ABE mass permeation flux by about 13 and 11� %, respectively.
    Keywords aqueous solutions ; butanol ; irradiation ; pervaporation ; renewable energy sources ; temperature ; ultrasonics
    Language English
    Dates of publication 2013-11
    Size p. 1159-1169.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 392344-7
    ISSN 0273-2289
    ISSN 0273-2289
    DOI 10.1007/s12010-013-0196-0
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  4. Article: Ultrasound-Enhanced Recovery of Butanol/ABE by Pervaporation

    Menchavez, Russel Navarro / Ha, Sung Ho

    Applied biochemistry and biotechnology

    Volume v. 171,, Issue no. 5

    Abstract: The search for renewable sources of energy has led to renewed interests on the biochemical route for the production of butanol. Butanol production suffers from several drawbacks, mainly caused by butanol inhibition to the butanol-producing microorganism ... ...

    Abstract The search for renewable sources of energy has led to renewed interests on the biochemical route for the production of butanol. Butanol production suffers from several drawbacks, mainly caused by butanol inhibition to the butanol-producing microorganism which makes it economically uncompetitive against the chemical process. One possible solution proposed is the in situ recovery of acetone–butanol–ethanol (ABE). Among the in situ recovery options, membrane processes like pervaporation have a great potential. Thus, the effects of temperature, feed concentration, and ultrasound irradiation on permeate concentration and permeation flux for the recovery of butanol/ABE by pervaporation from aqueous solutions were investigated in this study. In the butanol–water system, permeate butanol concentration as well as flux increased with an increase in temperature and butanol feed concentration. When pervaporation studies with ABE–water mixture were carried out at 60� °C for 2, 4, 8, 16, and 24� h, pervaporation profile revealed an optimal permeate concentration as well as permeation flux. Applications of ultrasound irradiation on pervaporation improved permeate concentration by about 23� g/L for both butanol and ABE. Ultrasound irradiation also improved butanol and ABE mass permeation flux by about 13 and 11� %, respectively.
    Keywords irradiation ; renewable energy sources ; aqueous solutions ; pervaporation ; butanol ; ultrasonics ; temperature
    Language English
    Document type Article
    ISSN 0273-2289
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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