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  1. Article ; Online: Time-dependent risk of fracture in adults with type 2 diabetes receiving anti-diabetic drug: A one-stage network meta-analysis.

    Shen, Yanjiao / Shi, Qingyang / Zou, Xinyu / Meng, Wentong / Tian, Haoming / Du, Liang / Li, Sheyu

    Diabetes/metabolism research and reviews

    2024  Volume 40, Issue 2, Page(s) e3780

    Abstract: Aims: To assess the time-dependent risk of fracture in adults with type 2 diabetes receiving anti-diabetic drugs.: Materials and methods: We searched MEDLINE, EMBASE, and Cochrane Library up to 18 November 2021, for randomized controlled trials (RCTs) ...

    Abstract Aims: To assess the time-dependent risk of fracture in adults with type 2 diabetes receiving anti-diabetic drugs.
    Materials and methods: We searched MEDLINE, EMBASE, and Cochrane Library up to 18 November 2021, for randomized controlled trials (RCTs) and propensity-score-matched non-randomized studies (NRSs) comparing all anti-diabetic drugs with standard treatment or with each other on fracture in adults with type 2 diabetes. The study performed a one-stage network meta-analysis using discrete-time hazard regression with reconstructed individual time-to-event data.
    Results: This network meta-analysis involved seven RCTs (65,051 adults with type 2 diabetes) with a median follow-up of 36 months and three propensity-score-based NRSs (17,954 participants) with a median follow-up of 27.3 months. Among anti-diabetic drugs, thiazolidinediones increased the overall hazard of fracture by 42% (95% credible interval [CrI], 3%-97%) and almost tripled the risk after 4 years (hazard ratio [HR], 2.74; 95% CrI, 1.53-4.80). Credible subgroup analysis suggested that thiazolidinediones increased the hazard of fracture only in females (HR, 2.19; 95% CrI, 1.26-3.74) but not among males (HR, 0.81; 95% CrI, 0.45-1.40). Moderate certainty evidence established that thiazolidinediones increase 92 fractures in five years per 1000 female patients. We did not find the risk of fractures with other anti-diabetic drugs including metformin, sulfonylureas, sodium-glucose cotransporter-2 (SGLT2) inhibitors, and dipeptidyl peptidase-4 (DPP-4) inhibitors.
    Conclusions: Long-term use of thiazolidinediones elevates the risk of fracture among females with type 2 diabetes. There is no evidence eliciting fracture risk associated with other anti-diabetic drugs.
    MeSH term(s) Male ; Adult ; Female ; Humans ; Hypoglycemic Agents/adverse effects ; Network Meta-Analysis ; Diabetes Mellitus, Type 2/complications ; Diabetes Mellitus, Type 2/drug therapy ; Diabetes Mellitus, Type 2/chemically induced ; Dipeptidyl-Peptidase IV Inhibitors/adverse effects ; Fractures, Bone/epidemiology ; Fractures, Bone/etiology ; Fractures, Bone/prevention & control ; Thiazolidinediones/adverse effects
    Chemical Substances Hypoglycemic Agents ; Dipeptidyl-Peptidase IV Inhibitors ; Thiazolidinediones
    Language English
    Publishing date 2024-02-17
    Publishing country England
    Document type Meta-Analysis ; Journal Article
    ZDB-ID 1470192-3
    ISSN 1520-7560 ; 1520-7552
    ISSN (online) 1520-7560
    ISSN 1520-7552
    DOI 10.1002/dmrr.3780
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: [Establishment of 14-Color Panel for Determining Leukocyte Subsets in Human Peripheral Blood with Flow Cytometry].

    Huang, Qiao-Rong / Jiang, Yan-Ni / Li, Hui-Fang / Li, Xue / Meng, Wen-Tong

    Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition

    2022  Volume 53, Issue 1, Page(s) 127–132

    Abstract: Objective: To establish a 14-color flow cytometry protocol for the examination of leukocyte subsets in human peripheral blood.: Methods: We used cell membrane surface antibodies CD45, CD3, CD4, CD8, CD19, CD56, CD16, CD14, CD25, CD127, HLA-DR, CD123, ...

    Abstract Objective: To establish a 14-color flow cytometry protocol for the examination of leukocyte subsets in human peripheral blood.
    Methods: We used cell membrane surface antibodies CD45, CD3, CD4, CD8, CD19, CD56, CD16, CD14, CD25, CD127, HLA-DR, CD123, CD11c and nucleus staining dye DAPI to establish a 14-color flow cytometry assay to determine the major cell subsets in human peripheral blood. We collected peripheral blood specimens from healthy volunteers to test for antibody titers and optimal photomultiplier tube (PMT) voltage, and to conduct single-color staining and fluorescence minus one control staining. After determining the test method and test conditions, the peripheral blood samples of 18 healthy volunteers were analyzed.
    Results: According to the cell classification and staining index, optimal antibody mass concentrations selected were as follows: CD25 and CD127 at 8.0 μg/mL, CD45, CD3, CD14 and CD123 at 4.0 μg/mL, CD8, CD19, CD56, CD16, HLA-DR and CD11c at 2.0 μg/mL, CD4 at 1.0 μg/mL and DAPI at 0.1 μg/mL. The detection voltages for CD45, CD3, CD4, CD8, CD19, CD56, CD16, CD14, CD25, CD127, HLA-DR, CD123, CD11c and DAPI were 450 V, 410 V, 400 V, 550 V, 405 V, 500 V, 520 V, 550 V, 550 V, 400 V, 450 V, 400 V, 580 V, and 300 V, respectively. The appropriate fluorescence compensation was determined by single-color staining and fluorescence minus one controls. The 14-color flow cytometry panel was established to analyze the main subsets of leukocytes in human peripheral blood, and peripheral blood samples from 18 healthy adults were examined, obtaining the percentages of each subset of peripheral blood leukocytes and the immunophenotypes of the main subsets.
    Conclusion: We established a 14-color panel for determining leukocyte subsets in human peripheral blood by flow cytometry, which produced stable and reliable results and was easy to operate.
    MeSH term(s) Cell Count ; Flow Cytometry ; Humans ; Immunophenotyping ; Leukocytes ; Lymphocyte Subsets
    Language Chinese
    Publishing date 2022-01-17
    Publishing country China
    Document type Journal Article
    ZDB-ID 2106840-9
    ISSN 1672-173X
    ISSN 1672-173X
    DOI 10.12182/20220160106
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Chloroquine Alleviates Atherosclerosis by Modulating Regulatory T Cells Through the ATM/AMPK/mTOR Signaling Pathway in ApoE -/- Mice.

    Liu, Dan / Zhang, Yonggang / Zhang, Yiyi / Huang, Qiaorong / Meng, Wentong / Gao, Jinhang / Mo, Xianming / Tian, Haoming / Li, Sheyu

    Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association

    2023  Volume 131, Issue 12, Page(s) 676–685

    Abstract: Background: Clinical observation suggests the atheroprotective effect of chloroquine and its derivatives, while its mechanism remains unclear. This study aimed to observe the protective effect of chloroquine against atherosclerosis and explore the ... ...

    Abstract Background: Clinical observation suggests the atheroprotective effect of chloroquine and its derivatives, while its mechanism remains unclear. This study aimed to observe the protective effect of chloroquine against atherosclerosis and explore the underlying mechanism.
    Methods: Ataxia telangiectasia mutated (ATM) wild-type or haploinsufficient apolipoprotein-E-knockout (ATM
    Results: In ATM
    Discussion: These findings suggest that chloroquine ameliorates atherosclerosis and stabilizes plaque by modulating Tregs differentiation through the regulation of the ATM/AMPK/mTOR pathway.
    MeSH term(s) Mice ; Animals ; T-Lymphocytes, Regulatory/metabolism ; Chloroquine/pharmacology ; Chloroquine/metabolism ; Chloroquine/therapeutic use ; AMP-Activated Protein Kinases/metabolism ; Ataxia Telangiectasia/drug therapy ; Ataxia Telangiectasia/metabolism ; Ataxia Telangiectasia/pathology ; Mice, Knockout, ApoE ; Atherosclerosis/drug therapy ; Atherosclerosis/prevention & control ; Signal Transduction ; TOR Serine-Threonine Kinases/metabolism ; Apolipoproteins E/metabolism ; Apolipoproteins E/pharmacology ; Apolipoproteins E/therapeutic use ; Mice, Inbred C57BL ; Plaque, Atherosclerotic/drug therapy ; Plaque, Atherosclerotic/metabolism ; Plaque, Atherosclerotic/pathology ; Mammals/metabolism
    Chemical Substances Chloroquine (886U3H6UFF) ; AMP-Activated Protein Kinases (EC 2.7.11.31) ; TOR Serine-Threonine Kinases (EC 2.7.11.1) ; Apolipoproteins E
    Language English
    Publishing date 2023-12-06
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1225416-2
    ISSN 1439-3646 ; 0947-7349
    ISSN (online) 1439-3646
    ISSN 0947-7349
    DOI 10.1055/a-2201-8728
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Colorectal cancer cells secreting DKK4 transform fibroblasts to promote tumour metastasis.

    Li, Xue / Chen, Yulin / Lu, Ran / Hu, Min / Gu, Lei / Huang, Qiaorong / Meng, Wentong / Zhu, Hongyan / Fan, Chuanwen / Zhou, Zongguang / Mo, Xianming

    Oncogene

    2024  

    Abstract: Wnt/β-catenin signalling is aberrantly activated in most colorectal cancer (CRC) and is one key driver involved in the initiation and progression of CRC. However, mutations of APC gene in CRC patients retain certain activity of APC protein with decreased ...

    Abstract Wnt/β-catenin signalling is aberrantly activated in most colorectal cancer (CRC) and is one key driver involved in the initiation and progression of CRC. However, mutations of APC gene in CRC patients retain certain activity of APC protein with decreased β-catenin signalling and DKK4 expression significantly upregulates and represses Wnt/β-catenin signalling in human CRC tissues, suggesting that a precisely modulated activation of the Wnt/β-catenin pathway is essential for CRC formation and progression. The underlying reasons why a specifically reduced degree, not a fully activating degree, of β-catenin signalling in CRC are unclear. Here, we showed that a soluble extracellular inhibitor of Wnt/β-catenin signalling, DKK4, is an independent factor for poor outcomes in CRC patients. DKK4 secreted from CRC cells inactivates β-catenin in fibroblasts to induce the formation of stress fibre-containing fibroblasts and myofibroblasts in culture conditions and in mouse CRC xenograft tissues, resulting in restricted expansion in tumour masses at primary sites and enhanced CRC metastasis in mouse models. Reduced β-catenin activity by a chemical inhibitor MSAB promoted the CRC metastasis. Our findings demonstrate why reduced β-catenin activity is needed for CRC progression and provide a mechanism by which interactions between CRC cells and stromal cells affect disease promotion.
    Language English
    Publishing date 2024-03-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 639046-8
    ISSN 1476-5594 ; 0950-9232
    ISSN (online) 1476-5594
    ISSN 0950-9232
    DOI 10.1038/s41388-024-03008-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Genetic insights into across pancreatitis types: the causal influence of immunoglobulin G N-glycosylation variants on disease risk.

    Chen, Yulin / Li, Xue / Lu, Ran / Lv, Yinchun / Ye, Junman / Huang, Qiaorong / Meng, Wentong / Long, Feiwu / Burman, Jonas / Mo, Xianming / Fan, Chuanwen

    Frontiers in immunology

    2024  Volume 15, Page(s) 1326370

    Abstract: Background: While a few case-control studies indicated a possible correlation of IgG N-glycosylation patterns with pancreatitis, their restricted sample sizes and methodologies prevented conclusive insights into causality or distinguishing traits across ...

    Abstract Background: While a few case-control studies indicated a possible correlation of IgG N-glycosylation patterns with pancreatitis, their restricted sample sizes and methodologies prevented conclusive insights into causality or distinguishing traits across pancreatitis types.
    Method: We conducted a two-sample Mendelian Randomization (MR) analysis to investigate the causal relationship between 77 IgG N-glycosylation traits and various types of pancreatitis, including acute pancreatitis (AP), chronic pancreatitis (CP), alcohol acute pancreatitis (AAP), and alcohol chronic pancreatitis (ACP). This analysis utilized summary-level data from genome-wide association studies (GWAS), employing methods such as IVW, MR-Egger, and weighted median. To ensure the robustness of our findings, several sensitivity analyses, including Cochran's Q statistic, leave-one-out, MR-Egger intercept, and MR-PRESSO global test were conducted.
    Result: Our study uncovered the causal relationship between specific IgG N-glycosylation traits and various types of pancreatitis. Notably, an increase in genetically predicted IGP7 levels was associated with a decreased risk of developing AP. For CP, our data suggested a protective effect associated with higher levels of both IGP7 and IGP31, contrasting with increased levels of IGP27 and IGP65, which were linked to a heightened risk. Moreover, in the case of AAP, elevated IGP31 levels were causatively associated with a lower incidence, while higher IGP26 levels correlated with an increased risk for ACP.
    Conclusion: This study establishes causal relationship between specific IgG N-glycosylation patterns and varying risks of different pancreatitis forms, underscoring their potential as predictive biomarkers. These findings necessitate further exploration into the underlying mechanisms, promising to inform more personalized diagnostic and therapeutic strategies in pancreatitis management.
    MeSH term(s) Humans ; Acute Disease ; Ethanol ; Genome-Wide Association Study ; Glycosylation ; Immunoglobulin G ; Pancreatitis, Chronic/genetics ; Mendelian Randomization Analysis
    Chemical Substances Ethanol (3K9958V90M) ; Immunoglobulin G
    Language English
    Publishing date 2024-03-18
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2024.1326370
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Computational flow cytometric analysis to detect epidermal subpopulations in human skin.

    Zhang, Lidan / Cen, Ying / Huang, Qiaorong / Li, Huifang / Mo, Xianming / Meng, Wentong / Chen, Junjie

    Biomedical engineering online

    2021  Volume 20, Issue 1, Page(s) 22

    Abstract: Background: The detection and dissection of epidermal subgroups could lead to an improved understanding of skin homeostasis and wound healing. Flow cytometric analysis provides an effective method to detect the surface markers of epidermal cells while ... ...

    Abstract Background: The detection and dissection of epidermal subgroups could lead to an improved understanding of skin homeostasis and wound healing. Flow cytometric analysis provides an effective method to detect the surface markers of epidermal cells while producing high-dimensional data files.
    Methods: A 9-color flow cytometric panel was optimized to reveal the heterogeneous subgroups in the epidermis of human skin. The subsets of epidermal cells were characterized using automated methods based on dimensional reduction approaches (viSNE) and clustering with Spanning-tree Progression Analysis of Density-normalized Events (SPADE).
    Results: The manual analysis revealed differences in epidermal distribution between body sites based on a series biaxial gating starting with the expression of CD49f and CD29. The computational analysis divided the whole epidermal cell population into 25 clusters according to the surface marker phenotype with SPADE. This automatic analysis delineated the differences between body sites. The consistency of the results was confirmed with PhenoGraph.
    Conclusion: A multicolor flow cytometry panel with a streamlined computational analysis pipeline is a feasible approach to delineate the heterogeneity of the epidermis in human skin.
    MeSH term(s) Algorithms ; Cluster Analysis ; Color ; Computer Simulation ; Epidermis/physiology ; Flow Cytometry/methods ; Humans ; Machine Learning ; Pattern Recognition, Automated ; Phenotype ; Skin/cytology ; Software
    Language English
    Publishing date 2021-02-17
    Publishing country England
    Document type Journal Article
    ISSN 1475-925X
    ISSN (online) 1475-925X
    DOI 10.1186/s12938-021-00858-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Imbalanced Innate Lymphoid Cells are Associated With Disease Activity and Arthritis Involvement in Patients With Systemic Lupus Erythematosus.

    Jiang, Yanni / Zhao, Yi / Liu, Yi / Huang, Qiaorong / Meng, Wentong / Xu, Hong / Mo, Xianming

    Archives of rheumatology

    2020  Volume 35, Issue 4, Page(s) 521–532

    Abstract: Objectives: This study aims to evaluate the frequency and absolute number of circulating innate lymphoid cell (ILC) subsets and their associations with clinical and serological features in systemic lupus erythematosus (SLE).: Patients and methods: We ...

    Abstract Objectives: This study aims to evaluate the frequency and absolute number of circulating innate lymphoid cell (ILC) subsets and their associations with clinical and serological features in systemic lupus erythematosus (SLE).
    Patients and methods: We recruited 28 SLE patients (6 males, 22 females; mean age 37.57 years; range, 18 to 56 years) and 13 healthy controls (4 males, 9 females; mean age 32.08 years; range, 19 to 48 years). Circulating ILC subsets were identified by flow cytometry. Associations between all detected cells and SLE disease activity, clinical manifestations, and serum autoantibodies were analyzed.
    Results: In this study, significantly higher frequencies of ILC2s and ILC3s, lower frequencies of ILC1s, and higher ILC1/ILC3 and ILC1/ILC2 ratios were observed in SLE patients than in healthy controls. The frequencies and number of ILC3s were positively associated with SLE disease activity index 2000 score and anti-double stranded deoxyribonucleic acid titers in patients with SLE. Decreased ILC1 frequencies, increased ILC3 frequencies, and decreased ILC1/ILC3 and ILC2/ILC3 ratios were observed in patients with arthritis compared to those without arthritis.
    Conclusion: Our results indicated biased altered distributions of circulating ILC subsets in SLE. ILC3s were associated with SLE disease activity, and ILC1s, ILC3s, and ILC1/ILC3 and ILC2/ILC3 ratios were associated with SLE accompanied with arthritis. Taken together, these results suggest that ILCs may serve as cellular biomarkers for disease activity and arthritis involvement in SLE.
    Language English
    Publishing date 2020-06-02
    Publishing country Turkey
    Document type Journal Article
    ZDB-ID 3012972-2
    ISSN 2618-6500 ; 2148-5046
    ISSN (online) 2618-6500
    ISSN 2148-5046
    DOI 10.46497/ArchRheumatol.2020.7440
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  8. Article ; Online: Dopey2 and Pcdh7 orchestrate the development of embryonic neural stem cells/ progenitors in zebrafish.

    Xiao, Yue / Hu, Min / Lin, Qiyan / Zhang, Ting / Li, Siying / Shu, Linjuan / Song, Xiuli / Xu, Xiaoyong / Meng, Wentong / Li, Xue / Xu, Hong / Mo, Xianming

    iScience

    2023  Volume 26, Issue 3, Page(s) 106273

    Abstract: DOPEY2 has been shown to be associated with Down syndrome and PCDH7 might be involved in Rett syndrome ... ...

    Abstract DOPEY2 has been shown to be associated with Down syndrome and PCDH7 might be involved in Rett syndrome and
    Language English
    Publishing date 2023-02-25
    Publishing country United States
    Document type Journal Article
    ISSN 2589-0042
    ISSN (online) 2589-0042
    DOI 10.1016/j.isci.2023.106273
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  9. Article: Gene expression networks involved in multiple cellular programs coexist in individual hepatocellular cancer cells.

    Zhao, Jin / Lu, Ran / Jin, Chen / Li, Siying / Chen, Yulin / Huang, Qiaorong / Li, Xue / Meng, Wentong / Wu, Hong / Wen, Tianfu / Mo, Xianming

    Heliyon

    2023  Volume 9, Issue 7, Page(s) e18305

    Abstract: The gene expression networks of a single cell can be used to reveal cell type- and condition-specific patterns that account for cell states, cell identity, and its responses to environmental changes. We applied single cell sequencing datasets to define ... ...

    Abstract The gene expression networks of a single cell can be used to reveal cell type- and condition-specific patterns that account for cell states, cell identity, and its responses to environmental changes. We applied single cell sequencing datasets to define mRNA patterns and visualized potential cellular capacities among hepatocellular cancer cells. The expressing numbers and levels of genes were highly heterogenous among the cancer cells. The cellular characteristics were dependent strongly on the expressing numbers and levels of genes, especially oncogenes and anti-oncogenes, in an individual cancer cell. The transcriptional activations of oncogenes and anti-oncogenes were strongly linked to inherent multiple cellular programs, some of which oppose and contend against other processes, in a cancer cell. The gene expression networks of multiple cellular programs proliferation, differentiation, apoptosis, autophagy, epithelial-mesenchymal transition, ATP production, and neurogenesis coexisted in an individual cancer cell. The findings give rise a hypothesis that a cancer cell expresses balanced combinations of genes and undergoes a given biological process by rapidly transmuting gene expressing networks.
    Language English
    Publishing date 2023-07-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e18305
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Identifying STRN3-RARA as a new fusion gene for acute promyelocytic leukemia.

    Zhang, Qi / Li, He / Chen, Xuelan / Gu, Fan / Zhang, Lanxin / Zhang, Lu / Chen, Tong / Chen, Qiang / Meng, Wentong / Wu, Yu / Chang, Hong / Liu, Ting / Chen, Chong / Ma, Hongbing / Liu, Yu

    Blood

    2023  Volume 142, Issue 17, Page(s) 1494–1499

    Abstract: Here we report a new fusion gene, STRN3-RARA, in acute promyelocytic leukemia (APL). It cooperates with UTX deficiency to drive full-blown APL in mice. Although STRN3-RARA leukemia quickly relapses after all-trans retinoic acid treatment, it can be ... ...

    Abstract Here we report a new fusion gene, STRN3-RARA, in acute promyelocytic leukemia (APL). It cooperates with UTX deficiency to drive full-blown APL in mice. Although STRN3-RARA leukemia quickly relapses after all-trans retinoic acid treatment, it can be restrained by cepharanthine.
    MeSH term(s) Animals ; Mice ; Leukemia, Promyelocytic, Acute/drug therapy ; Leukemia, Promyelocytic, Acute/genetics ; Oncogene Proteins, Fusion/genetics ; Tretinoin/therapeutic use
    Chemical Substances Oncogene Proteins, Fusion ; Tretinoin (5688UTC01R) ; Rara protein, mouse ; Strn3 protein, mouse
    Language English
    Publishing date 2023-03-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80069-7
    ISSN 1528-0020 ; 0006-4971
    ISSN (online) 1528-0020
    ISSN 0006-4971
    DOI 10.1182/blood.2023020619
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