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  1. Article ; Online: Molecular identification of an insulin-like peptide from the swimming crab Portunus trituberculatus and evidence for its glucoregulation function

    Rui Xu / Meng-en Wang / Shisheng Tu / Xi Xie / Dongfa Zhu

    Frontiers in Marine Science, Vol

    2023  Volume 10

    Abstract: Insulin-like peptides (ILPs) are essential to the animal kingdom for regulating growth and development, reproduction, behavior, metabolism, and lifespan. In crustaceans, the most well-known ILP is insulin-like androgenic gland hormone, a key hormone in ... ...

    Abstract Insulin-like peptides (ILPs) are essential to the animal kingdom for regulating growth and development, reproduction, behavior, metabolism, and lifespan. In crustaceans, the most well-known ILP is insulin-like androgenic gland hormone, a key hormone in regulating sex differentiation and reproduction. Identification of other ILPs and their functions are still limited. In this study, an insulin-like peptide gene of the swimming crab Portunus trituberculatus was cloned and characterized. Its transcripts were mainly found in nerve tissues and expression could be induced by glucose, implying a putative role in glucoregulation. After depletion of endogenous ILP, injection of ILP dsRNA (dsILP) significantly elevated blood glucose levels and recombinant ILP (rILP) decreased hemolymph glucose levels, further clarifying the involvement of acquired ILP in hemolymph glucose regulation. Injection of dsILP decreased PtAkt, PtGS, PtPFK and increased PtGSK and PtPEPCK gene expression. The opposite profile was observed after glucose and rILP injection, indicating that PtILP might negatively regulate hemolymph glucose levels via the IIS (insulin/IGF-1 signaling) pathway by inhibiting gluconeogenesis and promoting glycogen synthesis and glycolysis. This study has refined the mechanism of glucose regulation in crustaceans and laid the foundation for further studies on ILP function.
    Keywords Portunus trituberculatus ; insulin-like peptide ; glucoregulation ; IIS pathway ; RNAi ; Science ; Q ; General. Including nature conservation ; geographical distribution ; QH1-199.5
    Subject code 571
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Non-enzymatic glucose sensors based on controllable nanoporous gold/copper oxide nanohybrids

    Xiao, Xinxin / Hui Li / Meng’en Wang / Pengchao Si / Yichuan Pan

    Talanta. 2014 July 01, v. 125

    2014  

    Abstract: A kind of dealloyed nanoporous gold (NPG)/ultrathin CuO film nanohybrid for non-enzymatic glucose sensing has been prepared by a simple, in-situ, time-saving and controllable two-step electrodeposition. The three-dimensional and bicontinuous nanoporous ... ...

    Abstract A kind of dealloyed nanoporous gold (NPG)/ultrathin CuO film nanohybrid for non-enzymatic glucose sensing has been prepared by a simple, in-situ, time-saving and controllable two-step electrodeposition. The three-dimensional and bicontinuous nanoporous structure of the nanocomposites have been characterized by scanning electron microscope (SEM) and transmission electron microscopy (TEM), and the electrochemical tests have been estimated by cyclic voltammetry and single potential step chronoamperometry (SPSC). The optimal NPG/CuO electrode exhibits great electrocatalytic activity towards glucose oxidation and also shows obvious linear response to glucose up to 12mM with a high sensitivity of 374.0μAcm−2mM−1 and a good detection limit of 2.8μM (S/N=3), as well as strong tolerance against chloride poisoning and interference of ascorbic acid and uric acid.
    Keywords ascorbic acid ; chlorides ; cupric oxide ; electrochemistry ; electrodes ; glucose ; gold ; nanocomposites ; nanogold ; nanohybrids ; nanopores ; oxidation ; poisoning ; scanning electron microscopes ; scanning electron microscopy ; transmission electron microscopy ; uric acid
    Language English
    Dates of publication 2014-0701
    Size p. 366-371.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2014.03.030
    Database NAL-Catalogue (AGRICOLA)

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