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  1. Article ; Online: Genomics, Transcriptomics, and Epigenetics of Sporadic Pituitary Tumors.

    Marrero-Rodríguez, Daniel / Vela-Patiño, Sandra / Martinez-Mendoza, Florencia / Valenzuela-Perez, Alejandra / Peña-Martínez, Eduardo / Cano-Zaragoza, Amayrani / Kerbel, Jacobo / Andonegui-Elguera, Sergio / Glick-Betech, Shimon S / Hermoso-Mier, Karla X / Mercado-Medrez, Sophia / Moscona-Nissan, Alberto / Taniguchi-Ponciano, Keiko / Mercado, Moises

    Archives of medical research

    2023  Volume 54, Issue 8, Page(s) 102915

    Abstract: Pituitary tumors (PT) are highly heterogeneous neoplasms, comprising functioning and nonfunctioning lesions. Functioning PT include prolactinomas, causing amenorrhea-galactorrhea in women and sexual dysfunction in men; GH-secreting adenomas causing ... ...

    Abstract Pituitary tumors (PT) are highly heterogeneous neoplasms, comprising functioning and nonfunctioning lesions. Functioning PT include prolactinomas, causing amenorrhea-galactorrhea in women and sexual dysfunction in men; GH-secreting adenomas causing acromegaly-gigantism; ACTH-secreting corticotrophinomas causing Cushing disease (CD); and the rare TSH-secreting thyrotrophinomas that result in central hyperthyroidism. Nonfunctioning PT do not result in a hormonal hypersecretion syndrome and most of them are of gonadotrope differentiation; other non-functioning PT include null cell adenomas and silent ACTH-, GH- and PRL-adenomas. Less than 5% of PT occur in a familial or syndromic context whereby germline mutations of specific genes account for their molecular pathogenesis. In contrast, the more common sporadic PT do not result from a single molecular abnormality but rather emerge from several oncogenic events that culminate in an increased proliferation of pituitary cells, and in the case of functioning tumors, in a non-regulated hormonal hypersecretion. In recent years, important advances in the understanding of the molecular pathogenesis of PT have been made, including the genomic, transcriptomic, epigenetic, and proteomic characterization of these neoplasms. In this review, we summarize the available molecular information pertaining the oncogenesis of PT.
    MeSH term(s) Male ; Pregnancy ; Humans ; Female ; Pituitary Neoplasms/genetics ; Pituitary Neoplasms/pathology ; Proteomics ; Adenoma/genetics ; Adenoma/pathology ; Genomics ; Adrenocorticotropic Hormone/genetics ; Adrenocorticotropic Hormone/metabolism ; Gene Expression Profiling ; Epigenesis, Genetic
    Chemical Substances Adrenocorticotropic Hormone (9002-60-2)
    Language English
    Publishing date 2023-11-18
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 1156844-6
    ISSN 1873-5487 ; 0188-4409 ; 0188-0128
    ISSN (online) 1873-5487
    ISSN 0188-4409 ; 0188-0128
    DOI 10.1016/j.arcmed.2023.102915
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from POU1F1- and NR5A1-Cell Lineages

    Taniguchi-Ponciano, Keiko / Peña-Martínez, Eduardo / Silva-Román, Gloria / Vela-Patiño, Sandra / Guzman-Ortiz, Ana Laura / Quezada, Hector / Gomez-Apo, Erick / Chavez-Macias, Laura / Mercado-Medrez, Sophia / Vargas-Ortega, Guadalupe / Espinosa-de-los-Monteros, Ana Laura / Gonzales-Virla, Baldomero / Ferreira-Hermosillo, Aldo / Espinosa-Cardenas, Etual / Ramirez-Renteria, Claudia / Sosa, Ernesto / Lopez-Felix, Blas / Guinto, Gerardo / Marrero-Rodríguez, Daniel /
    Mercado, Moises

    Genes. 2020 Nov. 27, v. 11, no. 12

    2020  

    Abstract: Background: Pituitary adenomas (PA) are the second most common tumor in the central nervous system and have low counts of mutated genes. Splicing occurs in 95% of the coding RNA. There is scarce information about the spliceosome and mRNA-isoforms in PA, ... ...

    Abstract Background: Pituitary adenomas (PA) are the second most common tumor in the central nervous system and have low counts of mutated genes. Splicing occurs in 95% of the coding RNA. There is scarce information about the spliceosome and mRNA-isoforms in PA, and therefore we carried out proteomic and transcriptomic analysis to identify spliceosome components and mRNA isoforms in PA. Methods: Proteomic profile analysis was carried out by nano-HPLC and mass spectrometry with a quadrupole time-of-flight mass spectrometer. The mRNA isoforms and transcriptomic profiles were carried out by microarray technology. With proteins and mRNA information we carried out Gene Ontology and exon level analysis to identify splicing-related events. Results: Approximately 2000 proteins were identified in pituitary tumors. Spliceosome proteins such as SRSF1, U2AF1 and RBM42 among others were found in PA. These results were validated at mRNA level, which showed up-regulation of spliceosome genes in PA. Spliceosome-related genes segregate and categorize PA tumor subtypes. The PA showed alterations in CDK18 and THY1 mRNA isoforms which could be tumor specific. Conclusions: Spliceosome components are significant constituents of the PA molecular machinery and could be used as molecular markers and therapeutic targets. Splicing-related genes and mRNA-isoforms profiles characterize tumor subtypes.
    Keywords central nervous system ; exons ; gene expression ; gene ontology ; mass spectrometry ; microarray technology ; neoplasms ; proteomics ; spectrometers ; spliceosomes ; therapeutics ; transcriptomics
    Language English
    Dates of publication 2020-1127
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes11121422
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from

    Taniguchi-Ponciano, Keiko / Peña-Martínez, Eduardo / Silva-Román, Gloria / Vela-Patiño, Sandra / Guzman-Ortiz, Ana Laura / Quezada, Hector / Gomez-Apo, Erick / Chavez-Macias, Laura / Mercado-Medrez, Sophia / Vargas-Ortega, Guadalupe / Espinosa-de-Los-Monteros, Ana Laura / Gonzales-Virla, Baldomero / Ferreira-Hermosillo, Aldo / Espinosa-Cardenas, Etual / Ramirez-Renteria, Claudia / Sosa, Ernesto / Lopez-Felix, Blas / Guinto, Gerardo / Marrero-Rodríguez, Daniel /
    Mercado, Moises

    Genes

    2020  Volume 11, Issue 12

    Abstract: Background: Pituitary adenomas (PA) are the second most common tumor in the central nervous system and have low counts of mutated genes. Splicing occurs in 95% of the coding RNA. There is scarce information about the spliceosome and mRNA-isoforms in PA, ...

    Abstract Background: Pituitary adenomas (PA) are the second most common tumor in the central nervous system and have low counts of mutated genes. Splicing occurs in 95% of the coding RNA. There is scarce information about the spliceosome and mRNA-isoforms in PA, and therefore we carried out proteomic and transcriptomic analysis to identify spliceosome components and mRNA isoforms in PA.
    Methods: Proteomic profile analysis was carried out by nano-HPLC and mass spectrometry with a quadrupole time-of-flight mass spectrometer. The mRNA isoforms and transcriptomic profiles were carried out by microarray technology. With proteins and mRNA information we carried out Gene Ontology and exon level analysis to identify splicing-related events.
    Results: Approximately 2000 proteins were identified in pituitary tumors. Spliceosome proteins such as
    Conclusions: Spliceosome components are significant constituents of the PA molecular machinery and could be used as molecular markers and therapeutic targets. Splicing-related genes and mRNA-isoforms profiles characterize tumor subtypes.
    MeSH term(s) Adenoma/genetics ; Adenoma/metabolism ; Adenoma/pathology ; Alternative Splicing ; Biomarkers, Tumor ; Cell Lineage ; Chromatography, High Pressure Liquid ; Exons/genetics ; Gene Ontology ; Hormones/analysis ; Humans ; Nanotechnology ; Neoplasm Proteins/biosynthesis ; Neoplasm Proteins/genetics ; Oligonucleotide Array Sequence Analysis ; Pituitary Neoplasms/genetics ; Pituitary Neoplasms/metabolism ; Pituitary Neoplasms/pathology ; Principal Component Analysis ; Protein Isoforms/biosynthesis ; Protein Isoforms/genetics ; Proteome ; RNA, Messenger/biosynthesis ; RNA, Neoplasm/biosynthesis ; Spliceosomes ; Steroidogenic Factor 1/genetics ; Tandem Mass Spectrometry ; Transcription Factor Pit-1/genetics ; Transcription Factors/analysis ; Transcriptome
    Chemical Substances Biomarkers, Tumor ; Hormones ; NR5A1 protein, human ; Neoplasm Proteins ; POU1F1 protein, human ; Protein Isoforms ; Proteome ; RNA, Messenger ; RNA, Neoplasm ; Steroidogenic Factor 1 ; Transcription Factor Pit-1 ; Transcription Factors
    Language English
    Publishing date 2020-11-27
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes11121422
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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