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  1. Article ; Online: Anion-Binding Properties of Aliphatic Symmetric Squaramide Receptors.

    Mert, Serap / Erdebil, Özden

    ACS omega

    2024  Volume 9, Issue 7, Page(s) 8333–8342

    Abstract: Squaramides (SQs), which are very popular for their H-bonding ability, have attracted great interest due to their wide range of applications such as asymmetric synthesis, pharmacology, and anion transportation. In this study, aliphatic symmetric SQs ... ...

    Abstract Squaramides (SQs), which are very popular for their H-bonding ability, have attracted great interest due to their wide range of applications such as asymmetric synthesis, pharmacology, and anion transportation. In this study, aliphatic symmetric SQs based on
    Language English
    Publishing date 2024-02-09
    Publishing country United States
    Document type Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.3c09094
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Interactions of alumina and polystyrene nanoparticles with the innate immune system of

    Demirtürk, Zülbiye / Uçkan, Fevzi / Mert, Serap

    Drug and chemical toxicology

    2023  , Page(s) 1–13

    Abstract: Nowadays, particularly metallic, and polymeric nanoparticles (NPs) are widely produced and used in many fields. Due to the increase in both their usage and diversity, their release and accumulation in the environment are also accelerating. Therefore, ... ...

    Abstract Nowadays, particularly metallic, and polymeric nanoparticles (NPs) are widely produced and used in many fields. Due to the increase in both their usage and diversity, their release and accumulation in the environment are also accelerating. Therefore, their interactions with cells, especially immune cells, and their health risks are not fully understood. The impacts of metallic alumina (Al) NPs and polystyrene (PS) NPs obtained after the polymerization of carcinogenic styrene on living organisms have not yet been elucidated.
    Language English
    Publishing date 2023-06-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 548368-2
    ISSN 1525-6014 ; 0148-0545
    ISSN (online) 1525-6014
    ISSN 0148-0545
    DOI 10.1080/01480545.2023.2217484
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1380: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  3. Article ; Online: Production of alginate macrocapsule device for long-term normoglycaemia in the treatment of type 1 diabetes mellitus with pancreatic cell sheet engineering.

    Duman, Büşra Öncel / Yazir, Yusufhan / Halbutoğullari, Zehra Seda / Mert, Serap / Öztürk, Ahmet / Gacar, Gülçin / Duruksu, Gökhan

    Biomedical materials (Bristol, England)

    2024  Volume 19, Issue 2

    Abstract: Type 1 diabetes-mellitus (T1DM) is characterized by damage of beta cells in pancreatic islets. Cell-sheet engineering, one of the newest therapeutic approaches, has also been used to create functional islet systems by creating islet/beta cell-sheets and ... ...

    Abstract Type 1 diabetes-mellitus (T1DM) is characterized by damage of beta cells in pancreatic islets. Cell-sheet engineering, one of the newest therapeutic approaches, has also been used to create functional islet systems by creating islet/beta cell-sheets and transferring these systems to areas that require minimally invasive intervention, such as extrahepatic areas. Since islets, beta cells, and pancreas transplants are allogeneic, immune problems such as tissue rejection occur after treatment, and patients become insulin dependent again. In this study, we aimed to design the most suitable cell-sheet treatment method and macrocapsule-device that could provide long-term normoglycemia in rats. Firstly, mesenchymal stem cells (MSCs) and beta cells were co-cultured in a temperature-responsive culture dish to obtain a cell-sheet and then the cell-sheets macroencapsulated using different concentrations of alginate. The mechanical properties and pore sizes of the macrocapsule-device were characterized. The viability and activity of cell-sheets in the macrocapsule were evaluated
    MeSH term(s) Rats ; Humans ; Animals ; Diabetes Mellitus, Type 1/therapy ; Islets of Langerhans Transplantation/methods ; Blood Glucose/metabolism ; Alginates ; Islets of Langerhans ; Insulin/metabolism
    Chemical Substances Blood Glucose ; Alginates ; Insulin
    Language English
    Publishing date 2024-01-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 2265222-X
    ISSN 1748-605X ; 1748-6041
    ISSN (online) 1748-605X
    ISSN 1748-6041
    DOI 10.1088/1748-605X/ad1c9b
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6626: Show issues Location:
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  4. Article ; Online: Investigation of the differentiation potential of pericyte cells as an alternative source of mesenchymal stem cells.

    Polat, Selen / Yazir, Yusufhan / Duruksu, Gökhan / Kiliç, Kamil Can / Mert, Serap / Gacar, Gülçin / Öncel Duman, Büşra / Halbutoğullari, Zehra Seda

    Acta histochemica

    2024  Volume 126, Issue 3, Page(s) 152145

    Abstract: Background: The mesenchymal stem cells (MSCs) with characterized by their multipotency and capacity to differentiate into various tissue cell types, have led to their incorporation in regenerative medicine research. However, the limited numbers of MSCs ... ...

    Abstract Background: The mesenchymal stem cells (MSCs) with characterized by their multipotency and capacity to differentiate into various tissue cell types, have led to their incorporation in regenerative medicine research. However, the limited numbers of MSCs in the human body and their diverse differentiation capabilities in tissues highlight the need for exploring alternative regenerative cell sources. In this study, therefore, we conducted molecular level examinations to determine whether pericytes, specialized cell communities situated near blood vessels, could serve as a substitute for human bone marrow-derived mesenchymal stem cells (hBM-MSCs). In this context, the potential application of pericytes surrounds the vessels when MSCs are insufficient for functional purposes.
    Methods: The pericytes utilized in this investigation were derived from the placenta and characterized at the third passage. Similarly, the hBM-MSCs were also characterized at the third passage. The pluripotent properties of the two cell types were assessed at the gene expression level. Thereafter, both pericytes and hBM-MSCs were directed towards adipogenic, osteogenic and chondrogenic differentiation. The cells in both groups were examined on days 7, 14, and, 21 and their differentiation status was compared both immunohistochemically and through gene expression analysis.
    Results: Upon comparing the pluripotency characteristics of placental pericytes and hBM-MSCs, it was discovered that there was a substantial upregulation of the pluripotency genes FoxD3, Sox2, ZPF42, UTF1, and, Lin28 in both cell types. However, no significant expression of the genes Msx1, Nr6a1, Pdx1, and, GATA6 was observed in either cell type. It was also noted that pericytes differentiate into adipogenic, osteogenic and, chondrogenic lineages similar to hBM-MSCs.
    Discussion: As a result, it has been determined that pericytes exhibit high differentiation and proliferation properties similar to those of MSCs, and therefore can be considered a suitable alternative cell source for regenerative medicine and tissue engineering research, in cases where MSCs are not available or insufficient. It is notable that pericytes have been suggested as a potential substitute in studies where MSCs are lacking.
    Language English
    Publishing date 2024-03-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 77-2
    ISSN 1618-0372 ; 0065-1281
    ISSN (online) 1618-0372
    ISSN 0065-1281
    DOI 10.1016/j.acthis.2024.152145
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.

    Okcu, Alparslan / Yazir, Yusufhan / Şimşek, Turgay / Mert, Serap / Duruksu, Gökhan / Öztürk, Ahmet / Kiliç, Kamil Can / Akpinar, Gürler / Kasap, Murat

    Tissue & cell

    2023  Volume 82, Page(s) 102110

    Abstract: Objective: In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining ... ...

    Abstract Objective: In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
    Method: Pancreatic extracellular matrix was decellularized using different concentrations of detergent series. After the preparation of the protein-based tissue extracellular matrix was shown to be free of cells or any genetic material by molecular, immunofluorescence and histochemical techniques. Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules. Glucose-stimulated insulin secretion property of the islet cells in the microbeads was evaluated by insulin ELISA. The gene expression profile of the encapsulated cells was analyzed by Real-Time PCR.
    Results: Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria. The protein matrix derived from pancreatic tissue was shown to support the growth and maintenance of the islet cells. When compared to the non-encapsulated pancreatic islet, the encapsulated cells demonstrate to be more efficient in terms of insulin expression.
    Conclusion: The extracellular pancreatic matrix obtained in this study was directly used as supplementary in the alginate-based microcapsule enhancing the cell survival. The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency. Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
    MeSH term(s) Rats ; Animals ; Capsules/metabolism ; Capsules/pharmacology ; Islets of Langerhans ; Insulin/metabolism ; Glucose/pharmacology ; Glucose/metabolism ; Mesenchymal Stem Cells/metabolism ; Alginates/chemistry ; Islets of Langerhans Transplantation
    Chemical Substances Capsules ; Insulin ; Glucose (IY9XDZ35W2) ; Alginates
    Language English
    Publishing date 2023-05-16
    Publishing country Scotland
    Document type Journal Article
    ZDB-ID 204424-9
    ISSN 1532-3072 ; 0040-8166
    ISSN (online) 1532-3072
    ISSN 0040-8166
    DOI 10.1016/j.tice.2023.102110
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1016: Show issues Location:
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  6. Article ; Online: Investigation of impacts of decellularized heart extracellular matrix and VEGF on cardiomyogenic differentiation of mesenchymal stem cell through Notch/Hedgehog signaling pathways.

    Kiliç, Kamil Can / Yazir, Yusufhan / Öztürk, Ahmet / Halbutoğullari, Zehra Seda / Mert, Serap / Gacar, Gülçin / Duruksu, Gökhan

    Tissue & cell

    2023  Volume 84, Page(s) 102195

    Abstract: Objective: Decellularization is the process to obtain natural scaffolds with tissue integrity and extracellular matrix components, and recellularization is used to produce tissue-like constructs with specific cell types. In this study, rat bone marrow- ... ...

    Abstract Objective: Decellularization is the process to obtain natural scaffolds with tissue integrity and extracellular matrix components, and recellularization is used to produce tissue-like constructs with specific cell types. In this study, rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) were cultured on decellularized heart extracellular matrix. These cells were then induced to differentiate into cardiomyogenic cells under the stimulatory effect of vascular endothelial growth factor (VEGF) and other chemicals. This study aimed to investigate the effect of the cardiac extracellular matrix and VEGF on cardiomyogenic differentiation in the context of the Notch and Hedgehog signaling pathways.
    Methods: Heart samples extracted from rats were decellularized by serial application of detergent to remove cells from the tissue, and then recellularized with rBM-MSCs. The recellularized tissue matrices were then analyzed for cardiomyogenesis. Cardiomyogenic differentiation was performed on decellularized heart extracellular matrix (ECM; three-dimensional scaffolds) and culture plates (two-dimensional cell culture system) for 28 days to understand the effects of the heart extracellular matrix. In addition, differentiation was induced with and without the stimulatory effect of VEGF to understand the effect of VEGF on cardiomyogenic differentiation of rBM-MSCs.
    Results: Immunofluorescence staining showed that decellularization of the heart was performed effectively and successfully. After decellularization process, the heart extracellular matrix was completely free of cells. It was observed that rBM-MSCs transplanted onto the heart extracellular matrix remained viable and proliferated for 21 days after recellularization. The rBM-MSCs promoted cardiomyogenic differentiation in the conventional differentiation medium but were inversely affected by both VEGF and heart extracellular matrix proteins. Lower expression of connexin43 and cardiac troponin I genes was observed in cells induced by either matrix proteins or VEGF, compared to cells differentiated by chemical agents alone.
    Conclusion: In this study, we investigated the effect of decellularized heart extracellular matrix and VEGF on cardiomyogenic differentiation of rBM-MSCs. On the decellularized cardiac extracellular matrix, rBM-MSCs maintained their viability by adhering to the matrix and proliferating further. The adhesion of the cells to the matrix also produced a physical stimulus that led to the formation of histological structures resembling myocardial layers. Chemical stimulation of the decellularized heart extracellular matrix and cardiomyogenic differentiation supplements resulted in increased expression of cardiomyogenic biomarkers through modulation of the Notch and Hedgehog signaling pathways.
    MeSH term(s) Rats ; Animals ; Tissue Scaffolds/chemistry ; Vascular Endothelial Growth Factor A/metabolism ; Hedgehog Proteins/analysis ; Hedgehog Proteins/metabolism ; Hedgehog Proteins/pharmacology ; Cell Differentiation ; Extracellular Matrix/metabolism ; Mesenchymal Stem Cells
    Chemical Substances Vascular Endothelial Growth Factor A ; Hedgehog Proteins
    Language English
    Publishing date 2023-08-08
    Publishing country Scotland
    Document type Journal Article
    ZDB-ID 204424-9
    ISSN 1532-3072 ; 0040-8166
    ISSN (online) 1532-3072
    ISSN 0040-8166
    DOI 10.1016/j.tice.2023.102195
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1016: Show issues Location:
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  7. Article: Poly(asymmetrical glycolide)s: The Mechanisms and Thermosensitive Properties

    Çetin, Duygu / Arıcan, Mehmet Onur / Kenar, Halime / Mert, Serap / Mert, Olcay

    Macromolecules. 2020 Dec. 14, v. 54, no. 1

    2020  

    Abstract: A new highly reactive isobutyl lactide monomer (IBL) was synthesized with the ring closing of halogenated carboxylic acid acquired by the reaction of (S)-2-hydroxy-4-methylpentanoic acid with 2-bromopropionyl bromide. A desired thermosensitive and ... ...

    Abstract A new highly reactive isobutyl lactide monomer (IBL) was synthesized with the ring closing of halogenated carboxylic acid acquired by the reaction of (S)-2-hydroxy-4-methylpentanoic acid with 2-bromopropionyl bromide. A desired thermosensitive and injectable PEG-based poly(isobutyl lactide) di- and triblock copolymers (MePEG-PIBL and PIBL-PEG-PIBL), a promising alternative to customary PEG-based PLA and PLGA block copolymers, have been synthesized with narrow polydispersities as low as 1.08, high conversions up to 99%, and suitable hydrophilic/hydrophobic balances for gel–sol applications by ring-opening polymerization at 120 °C. The polymerization rate of MePEG-PIBL was found to be the highest among other PEG-based substituted glycolides (MePEG-PDIBG, MePEG-PIPL, and MePEG-PDIPG) due to having a single and less hindered secondary β-carbon atom (R₂CH₂) in IBL. The mechanism of poly(asymmetrical glycolide)s was also illuminated by single-frequency decoupled ¹H NMR, ¹³C NMR, and optical rotation analyses by taking into account the effect of substituents on symmetrical and asymmetrical glycolides. PIBL units of the block copolymers showed an amorphous phase, critical for desired drug release rate, in thermal analyses. Thus, PIBL-based copolymer gels displayed a more effective release profile of paclitaxel (up to 57%) than semi-crystalline PLLA-PEG gels (up to 5.7%) in 2 weeks. The location of PEG, present as an internal or lateral component in copolymers, also affected the rate of hydrolitic degradation (34.6% vs 23.7% degradation of PIBL units in di- and triblock copolymers, respectively). According to the results of cell viability assays (WST-1 test and live/dead assay), where L929 and human primary dermal fibroblasts were tested, the triblock copolymer did not cause any cell damages or cell morphological changes in all concentration ranges tested (0.1–3.0 mg mL–¹). The aqueous solutions of these copolymers exhibited very well temperature-dependent reversible gel–sol transitions for use in a localized drug delivery system.
    Keywords carboxylic acids ; cell viability ; composite polymers ; drug delivery systems ; drugs ; fibroblasts ; humans ; hydrophilicity ; hydrophobicity ; paclitaxel ; polymerization ; thermosensitivity
    Language English
    Dates of publication 2020-1214
    Size p. 272-290.
    Publishing place American Chemical Society
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 1491942-4
    ISSN 1520-5835 ; 0024-9297
    ISSN (online) 1520-5835
    ISSN 0024-9297
    DOI 10.1021/acs.macromol.0c01893
    Database NAL-Catalogue (AGRICOLA)

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