Article ; Online: Microwave-Induced Transient Heating Accelerates Protein PEGylation.
Biomacromolecules
2023 Volume 24, Issue 6, Page(s) 2856–2863
Abstract: PEGylation is one of the most widely employed strategies to increase the circulatory half-life of proteins and to reduce immune responses. However, conventional PEGylation protocols often require excess reagents and extended reaction times because of ... ...
Abstract | PEGylation is one of the most widely employed strategies to increase the circulatory half-life of proteins and to reduce immune responses. However, conventional PEGylation protocols often require excess reagents and extended reaction times because of their inefficiency. This study demonstrates that a microwave-induced transient heating phenomenon can be exploited to significantly accelerate protein PEGylation and even increase the degree of PEGylation achievable beyond what is possible at room temperature. This can be accomplished under conditions that do not compromise protein integrity. Several PEGylation chemistries and proteins are tested, and mechanistic insight is provided. Under certain conditions, extremely high levels of PEGylation were achieved in a matter of minutes. Moreover, considering the significantly reduced reaction times, the microwave-induced transient heating concept was adapted for continuous flow manufacturing of bioconjugates. |
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MeSH term(s) | Microwaves ; Heating/methods ; Proteins |
Chemical Substances | Proteins |
Language | English |
Publishing date | 2023-05-02 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ISSN | 1526-4602 |
ISSN (online) | 1526-4602 |
DOI | 10.1021/acs.biomac.3c00250 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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