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  1. Book ; Thesis: Could changes in the thymic epithelial compartment account for an age-associated increase of autoimmunity?

    Michel, Chloé

    2014  

    Author's details [presented by Chloé Michel]
    Language English
    Size VII, 161 Bl. : Ill., graph. Darst.
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Heidelberg, Univ., Diss., 2014
    HBZ-ID HT018539005
    Database Catalogue ZB MED Medicine, Health

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  2. Article ; Online: What does this discount tell you?

    Michel, Chloé

    Michel : Chloé , p. 5-47

    investigating the impact of pre-promotion price effects

    2017  , Page(s) 5–47

    Author's details Chloé Michel
    Language English
    Size Online-Ressource
    Publishing place Zurich
    Document type Article ; Online
    Database ECONomics Information System

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  3. Book ; Online: How do people perceive information?

    Michel, Chloé

    three essays in empirical economics

    2017  

    Author's details presented by Chloé Michel (from Villars-Ste-Croix, VD)
    Language English
    Size 1 Online-Ressource (circa 111 Seiten), Illustrationen
    Publishing place Zurich
    Document type Book ; Online
    Note Enthält 3 Beiträge
    Database ECONomics Information System

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  4. Book ; Thesis: Could changes in the thymic epithelial compartment account for an age-associated increase of autoimmunity?

    Michel, Chloé

    2014  

    Author's details presented by Chloé Michel
    Language English
    Size VII, 161 S., Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Univ., Diss.--Heidelberg, 2014
    Note Zsfassung in dt. Sprache
    Database Former special subject collection: coastal and deep sea fishing

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  5. Article ; Online: The Importance of an Accurate Diagnosis for Renal Colic.

    Meltzer, Andrew C / Michel, Chloe

    Annals of emergency medicine

    2016  Volume 68, Issue 5, Page(s) 643–644

    MeSH term(s) Colic ; Humans ; Renal Colic
    Language English
    Publishing date 2016-10-22
    Publishing country United States
    Document type Letter ; Research Support, N.I.H., Extramural ; Comment
    ZDB-ID 603080-4
    ISSN 1097-6760 ; 0196-0644
    ISSN (online) 1097-6760
    ISSN 0196-0644
    DOI 10.1016/j.annemergmed.2016.06.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Risky lifestyle behaviors and breast cancer diagnosis

    Michel, Chloé / Sovinsky, Michelle / Stern, Steven N

    Michel : Chloé , p. 49-79

    2017  , Page(s) 49–79

    Author's details Chloé Michel, joint project with Michelle Sovinsky and Steven Stern
    Language English
    Size Online-Ressource
    Publishing place Zurich
    Document type Article ; Online
    Database ECONomics Information System

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  7. Article ; Online: An efficient protocol for in vivo labeling of proliferating epithelial cells.

    Michel, Chloé / Küchler, Rita / Martin, Iris / Kyewski, Bruno / Pinto, Sheena

    Journal of immunological methods

    2018  Volume 457, Page(s) 82–86

    Abstract: The study of organogenesis, tissue-homeostasis and regeneration requires the precise assessment of in vivo cell proliferation. To this end a host of methods have been developed to detect and quantify DNA synthesis in proliferating cells. These include ... ...

    Abstract The study of organogenesis, tissue-homeostasis and regeneration requires the precise assessment of in vivo cell proliferation. To this end a host of methods have been developed to detect and quantify DNA synthesis in proliferating cells. These include cell labeling with various nucleotide analogues and fluorescence reporter-based animal models with each method presenting its idiosyncratic shortcomings. Quantitative assessment of epithelial cell turnover has been partly hampered due to their variable and limited in vivo accessibility and the requirement for harsher isolation procedures to procure single cells for FACS analysis. Here, we report a reliable protocol to study in vivo cell proliferation of epithelial cells in mice by repeatedly injecting EdU intravenously for an extended 12-day period. EdU incorporation was quantitated ex vivo by FACS after tissue dissociation in order to obtain single epithelial cell suspensions. As a lead population, we analyzed thymic epithelial cells (TECs), where we were able to label compartmentalized TEC subsets to saturation without apparent toxic effects on the thymus architecture or stress-sensitive TEC lineage differentiation. The data is in concordance with the prevailing model of medullary TEC terminal differentiation that includes the post-Aire stage. The same protocol was successfully applied to epithelial cells of various other organs - skin, lymph node, kidney and small intestine - tissues with widely varying frequencies and rates of proliferating epithelial cells.
    MeSH term(s) Administration, Intravenous ; Animals ; Cell Count ; Cell Differentiation ; Cell Proliferation ; Deoxyuridine/administration & dosage ; Deoxyuridine/analogs & derivatives ; Epithelial Cells/cytology ; Flow Cytometry ; Mice ; Mice, Inbred C57BL ; Staining and Labeling/methods ; Thymus Gland/cytology
    Chemical Substances Deoxyuridine (W78I7AY22C)
    Language English
    Publishing date 2018-03-30
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 120142-6
    ISSN 1872-7905 ; 0022-1759
    ISSN (online) 1872-7905
    ISSN 0022-1759
    DOI 10.1016/j.jim.2018.03.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The Treatment of Genitoperineal Hidradenitis Suppurativa: A Review of the Literature.

    Michel, Chloe / DiBianco, John Michael / Sabarwal, Vikram / Stein, Daniel M

    Urology

    2018  Volume 124, Page(s) 1–5

    Abstract: Hidradenitis suppurativa is a chronic inflammatory condition affecting the axilla, genitals, perineum, and perianal regions. The pathophysiology of hidradenitis suppurativa is complex and requires a multidisciplinary approach to treatment involving ... ...

    Abstract Hidradenitis suppurativa is a chronic inflammatory condition affecting the axilla, genitals, perineum, and perianal regions. The pathophysiology of hidradenitis suppurativa is complex and requires a multidisciplinary approach to treatment involving medical and surgical management when indicated. We describe our multidisciplinary protocol for treatment, which includes rheumatology-monitored immunotherapy, medical management, wide surgical resection, wound care, and reconstruction. The multidisciplinary care team includes rheumatology, wound care, and reconstructive urologic surgery. Surgical management includes wide local surgical resection, negative pressure dressing, delayed reconstruction, and perioperative immunotherapy. Multimodal treatment with surgical, medical, wound, and immunotherapy care is vital to successful treatment.
    MeSH term(s) Female ; Genital Diseases, Female/therapy ; Genital Diseases, Male/therapy ; Hidradenitis Suppurativa/therapy ; Humans ; Male ; Perineum
    Language English
    Publishing date 2018-11-02
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 192062-5
    ISSN 1527-9995 ; 0090-4295
    ISSN (online) 1527-9995
    ISSN 0090-4295
    DOI 10.1016/j.urology.2018.10.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: An efficient protocol for in vivo labeling of proliferating epithelial cells

    Michel, Chloé / Bruno Kyewski / Iris Martin / Rita Küchler / Sheena Pinto

    Journal of immunological methods. 2018,

    2018  

    Abstract: The study of organogenesis, tissue-homeostasis and regeneration requires the precise assessment of in vivo cell proliferation. To this end a host of methods have been developed to detect and quantify DNA synthesis in proliferating cells. These include ... ...

    Abstract The study of organogenesis, tissue-homeostasis and regeneration requires the precise assessment of in vivo cell proliferation. To this end a host of methods have been developed to detect and quantify DNA synthesis in proliferating cells. These include cell labeling with various nucleotide analogues and fluorescence reporter-based animal models with each method presenting its idiosyncratic shortcomings. Quantitative assessment of epithelial cell turnover has been partly hampered due to their variable and limited in vivo accessibility and the requirement for harsher isolation procedures to procure single cells for FACS analysis. Here, we report a reliable protocol to study in vivo cell proliferation of epithelial cells in mice by repeatedly injecting EdU intravenously for an extended 12-day period. EdU incorporation was quantitated ex vivo by FACS after tissue dissociation in order to obtain single epithelial cell suspensions. As a lead population, we analyzed thymic epithelial cells (TECs), where we were able to label compartmentalized TEC subsets to saturation without apparent toxic effects on the thymus architecture or stress-sensitive TEC lineage differentiation. The data is in concordance with the prevailing model of medullary TEC terminal differentiation that includes the post-Aire stage. The same protocol was successfully applied to epithelial cells of various other organs – skin, lymph node, kidney and small intestine – tissues with widely varying frequencies and rates of proliferating epithelial cells.
    Keywords animal models ; cell proliferation ; cell suspension culture ; dissociation ; DNA replication ; epithelial cells ; fluorescence ; intravenous injection ; kidneys ; lymph nodes ; mice ; organogenesis ; protocols ; small intestine ; thymus gland ; tissues ; toxicity
    Language English
    Size p. .
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 120142-6
    ISSN 1872-7905 ; 0022-1759
    ISSN (online) 1872-7905
    ISSN 0022-1759
    DOI 10.1016/j.jim.2018.03.015
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: Advertising and life satisfaction

    Michel, Chloé / Oswald, Andrew J / Proto, Eugenio / Sovinsky, Michelle

    Michel : Chloé , p. 81-98

    cross-national evidence on one million Europeans

    2017  , Page(s) 81–98

    Author's details Chloé Michel, joint project with Michelle Sovinsky, Eugenio Proto, and Andrew J. Oswald
    Language English
    Size Online-Ressource
    Publishing place Zurich
    Document type Article ; Online
    Database ECONomics Information System

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