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  1. Article: Evaluation and comparison of structurally different cellulose-based hemostatic agents in a rat kidney model

    Paprskářová, Alice / Suchý, Pavel / Chalupová, Marta / Michlovská, Lenka / Klusáková, Jarmila / Sopuch, Tomáš / Vojtová, Lucy

    Cellulose. 2021 Sept., v. 28, no. 14

    2021  

    Abstract: Different topical hemostatic materials are used to achieve effective hemostasis. High hemostatic activity, biocompatibility, bioresorbability, and easy manipulation are to be expected in such a developed product. In the surgical world with these specific ...

    Abstract Different topical hemostatic materials are used to achieve effective hemostasis. High hemostatic activity, biocompatibility, bioresorbability, and easy manipulation are to be expected in such a developed product. In the surgical world with these specific requirements, finding a proper hemostatic agent is very difficult. The study compared several materials of various construction properties, which were assessed for structural and related properties by morphological analyses and assessed in vivo for their efficiency and behaviour using a model of rat partial nephrectomy. New sodium salt of carboxymethyl cellulose (CMC) sponge with the lowest porosity and free swell absorptive capacity contained the highest amount of hydroxyl and carboxyl groups. Results revealed that this CMC material in the form of a bioresorbable sponge may ensure the necessary hemostatic effects, while also providing a positive influence on the reaction of the local tissue. The CMC material also needed significantly less time to achieve hemostasis (p < 0.001). Moreover, the sponge reached satisfactory results in the histopathological evaluation with the lowest destruction score and favorable healing reaction. This modified product proved itself to be a promising bioresorbable hemostat, which, according to its design, matches with its surgical applications. In general, the obtained data elucidated the dependency of the total effect on its structure and composition.
    Keywords biocompatibility ; carboxymethylcellulose ; hemostasis ; histopathology ; kidneys ; models ; porosity ; rats ; sodium
    Language English
    Dates of publication 2021-09
    Size p. 9369-9382.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 1496831-9
    ISSN 1572-882X ; 0969-0239
    ISSN (online) 1572-882X
    ISSN 0969-0239
    DOI 10.1007/s10570-021-04104-1
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Accelular nanofibrous bilayer scaffold intrapenetrated with polydopamine network and implemented into a full-thickness wound of a white-pig model affects inflammation and healing process.

    Kacvinská, Katarína / Pavliňáková, Veronika / Poláček, Petr / Michlovská, Lenka / Blahnová, Veronika Hefka / Filová, Eva / Knoz, Martin / Lipový, Břetislav / Holoubek, Jakub / Faldyna, Martin / Pavlovský, Zdeněk / Vícenová, Monika / Cvanová, Michaela / Jarkovský, Jiří / Vojtová, Lucy

    Journal of nanobiotechnology

    2023  Volume 21, Issue 1, Page(s) 80

    Abstract: Treatment of complete loss of skin thickness requires expensive cellular materials and limited skin grafts used as temporary coverage. This paper presents an acellular bilayer scaffold modified with polydopamine (PDA), which is designed to mimic a ... ...

    Abstract Treatment of complete loss of skin thickness requires expensive cellular materials and limited skin grafts used as temporary coverage. This paper presents an acellular bilayer scaffold modified with polydopamine (PDA), which is designed to mimic a missing dermis and a basement membrane (BM). The alternate dermis is made from freeze-dried collagen and chitosan (Coll/Chit) or collagen and a calcium salt of oxidized cellulose (Coll/CaOC). Alternate BM is made from electrospun gelatin (Gel), polycaprolactone (PCL), and CaOC. Morphological and mechanical analyzes have shown that PDA significantly improved the elasticity and strength of collagen microfibrils, which favorably affected swelling capacity and porosity. PDA significantly supported and maintained metabolic activity, proliferation, and viability of the murine fibroblast cell lines. The in vivo experiment carried out in a domestic Large white pig model resulted in the expression of pro-inflammatory cytokines in the first 1-2 weeks, giving the idea that PDA and/or CaOC trigger the early stages of inflammation. Otherwise, in later stages, PDA caused a reduction in inflammation with the expression of the anti-inflammatory molecule IL10 and the transforming growth factor β (TGFβ1), which could support the formation of fibroblasts. Similarities in treatment with native porcine skin suggested that the bilayer can be used as an implant for full-thickness skin wounds and thus eliminate the use of skin grafts.
    MeSH term(s) Swine ; Animals ; Mice ; Nanofibers ; Osmium Compounds ; Inflammation
    Chemical Substances polydopamine ; chloropentaammineosmium(III) chloride (39176-94-8) ; Osmium Compounds
    Language English
    Publishing date 2023-03-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 2100022-0
    ISSN 1477-3155 ; 1477-3155
    ISSN (online) 1477-3155
    ISSN 1477-3155
    DOI 10.1186/s12951-023-01822-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Mutual influence of selenium nanoparticles and FGF2-STAB

    Muchová, Johana / Hearnden, Vanessa / Michlovská, Lenka / Vištejnová, Lucie / Zavaďáková, Anna / Šmerková, Kristýna / Kočiová, Silvia / Adam, Vojtěch / Kopel, Pavel / Vojtová, Lucy

    Journal of nanobiotechnology

    2021  Volume 19, Issue 1, Page(s) 103

    Abstract: In a biological system, nanoparticles (NPs) may interact with biomolecules. Specifically, the adsorption of proteins on the nanoparticle surface may influence both the nanoparticles' and proteins' overall bio-reactivity. Nevertheless, our knowledge of ... ...

    Abstract In a biological system, nanoparticles (NPs) may interact with biomolecules. Specifically, the adsorption of proteins on the nanoparticle surface may influence both the nanoparticles' and proteins' overall bio-reactivity. Nevertheless, our knowledge of the biocompatibility and risk of exposure to nanomaterials is limited. Here, in vitro and ex ovo biocompatibility of naturally based crosslinked freeze-dried 3D porous collagen/chitosan scaffolds, modified with thermostable fibroblast growth factor 2 (FGF2-STAB
    MeSH term(s) Animals ; Anti-Bacterial Agents ; Biocompatible Materials/pharmacology ; Cell Line ; Chitosan/pharmacology ; Collagen/pharmacology ; Fibroblast Growth Factor 2/pharmacology ; Fibroblasts/drug effects ; Humans ; Materials Testing ; Nanoparticles/chemistry ; Nanoparticles/therapeutic use ; Porosity ; Selenium/chemistry ; Selenium/pharmacology ; Tissue Engineering/methods ; Tissue Scaffolds ; Wound Healing
    Chemical Substances Anti-Bacterial Agents ; Biocompatible Materials ; Fibroblast Growth Factor 2 (103107-01-3) ; Collagen (9007-34-5) ; Chitosan (9012-76-4) ; Selenium (H6241UJ22B)
    Language English
    Publishing date 2021-04-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2100022-0
    ISSN 1477-3155 ; 1477-3155
    ISSN (online) 1477-3155
    ISSN 1477-3155
    DOI 10.1186/s12951-021-00849-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Ex-vivo biomechanical testing of pig femur diaphysis B type fracture fixed by novel biodegradable bone glue.

    Krtička, Milan / Michlovská, Lenka / Nekuda, Vladimír / Poláček, Petr / Valová, Kristýna / Žídek, Jan / Kaiser, Jozef / Zikmund, Tomáš / Vojtová, Lucy

    Journal of the mechanical behavior of biomedical materials

    2020  Volume 115, Page(s) 104249

    Abstract: Aims: The aim of this study was to answer the question whether our newly developed injectable biodegradable "self-setting" polymer-composite as a bone adhesive is a good "bone-glue" candidate to efficiently fix comminuted fractures of pig femoral bones ... ...

    Abstract Aims: The aim of this study was to answer the question whether our newly developed injectable biodegradable "self-setting" polymer-composite as a bone adhesive is a good "bone-glue" candidate to efficiently fix comminuted fractures of pig femoral bones used as an ex-vivo experimental model.
    Methods: Mechanical properties of adhesive prepared from α-tricalcium phosphate (TCP) powder and thermogelling copolymer were optimized by selecting the appropriate composition with adhesion enhancers based on dopamine and sodium iodinate. Setting time and injectability were controlled by rheology. Ex-vivo experiments of fixed pig bones were provided in terms of either the three-point bending test of bending wedge type fractured pig femurs (with LCP) or the axial compression test of 45° oblique fractured femurs (without LCP) in physiological saline solution at 37 °C. Fractured bones treated with optimized adhesive before and after bending tests were imaged by X-ray microtomography (μCT).
    Results: Based on the rheological measurement, the adhesive modified with both dopamine and sodium iodinate exhibited optimal thixotropic properties required for injection via thin 22 G needle. This optimal adhesive composition showed an 8 min lag phase (processing time) followed by fast increase in storage modulus at 37 °C up to 1 GPa within 110 min. Self-setting of dopamine/iodinate modified adhesive was completed in 48 h exhibiting the maximum strength at compression of 7.98 MPa ± 1.39 MPa. Whereas unmodified adhesive failed in glue-to-bone adhesion, dopamine and dopamine/iodinate modified adhesive used for 45° oblique fracture fixation showed good and similar strength at compression (3.05 and 2.79 MPa, respectively). However, significantly higher elasticity of about 250% exhibited adhesive with iodinate enhancer. Moreover, mechanical properties of B2 fractures fixed with both LCP and dopamine/iodinate adhesive were approaching closely to the properties of original bone. Excellent adhesion between the adhesive and the bone fragments was proved by μCT.
    Conclusion: The polymer-composite bone adhesive modified with dopamine/iodinate exhibited very good fixation ability of femoral artificial comminuted fractures in an experimental model.
    MeSH term(s) Animals ; Biomechanical Phenomena ; Bone Cements ; Bone Plates ; Diaphyses ; Femoral Fractures/diagnostic imaging ; Femur/diagnostic imaging ; Fracture Fixation ; Fracture Fixation, Internal ; Swine
    Chemical Substances Bone Cements
    Language English
    Publishing date 2020-12-05
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2378381-3
    ISSN 1878-0180 ; 1751-6161
    ISSN (online) 1878-0180
    ISSN 1751-6161
    DOI 10.1016/j.jmbbm.2020.104249
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: The Effect of the Thermosensitive Biodegradable PLGA⁻PEG⁻PLGA Copolymer on the Rheological, Structural and Mechanical Properties of Thixotropic Self-Hardening Tricalcium Phosphate Cement.

    Vojtova, Lucy / Michlovska, Lenka / Valova, Kristyna / Zboncak, Marek / Trunec, Martin / Castkova, Klara / Krticka, Milan / Pavlinakova, Veronika / Polacek, Petr / Dzurov, Matej / Lukasova, Vera / Rampichova, Michala / Suchy, Tomas / Sedlacek, Radek / Ginebra, Maria-Pau / Montufar, Edgar B

    International journal of molecular sciences

    2019  Volume 20, Issue 2

    Abstract: The current limitations of calcium phosphate cements (CPCs) used in the field of bone regeneration consist of their brittleness, low injectability, disintegration in body fluids and low biodegradability. Moreover, no method is currently available to ... ...

    Abstract The current limitations of calcium phosphate cements (CPCs) used in the field of bone regeneration consist of their brittleness, low injectability, disintegration in body fluids and low biodegradability. Moreover, no method is currently available to measure the setting time of CPCs in correlation with the evolution of the setting reaction. The study proposes that it is possible to improve and tune the properties of CPCs via the addition of a thermosensitive, biodegradable, thixotropic copolymer based on poly(lactic acid), poly(glycolic acid) and poly(ethylene glycol) (PLGA⁻PEG⁻PLGA) which undergoes gelation under physiological conditions. The setting times of alpha-tricalcium phosphate (α-TCP) mixed with aqueous solutions of PLGA⁻PEG⁻PLGA determined by means of time-sweep curves revealed a lag phase during the dissolution of the α-TCP particles. The magnitude of the storage modulus at lag phase depends on the liquid to powder ratio, the copolymer concentration and temperature. A sharp increase in the storage modulus was observed at the time of the precipitation of calcium deficient hydroxyapatite (CDHA) crystals, representing the loss of paste workability. The PLGA⁻PEG⁻PLGA copolymer demonstrates the desired pseudoplastic rheological behaviour with a small decrease in shear stress and the rapid recovery of the viscous state once the shear is removed, thus preventing CPC phase separation and providing good cohesion. Preliminary cytocompatibility tests performed on human mesenchymal stem cells proved the suitability of the novel copolymer/α-TCP for the purposes of mini-invasive surgery.
    MeSH term(s) Biocompatible Materials/chemistry ; Bone Cements/chemistry ; Calcium Phosphates/chemistry ; Cell Survival ; Cells, Cultured ; Humans ; Hydrogen-Ion Concentration ; Materials Testing ; Mechanical Phenomena ; Molecular Structure ; Polyesters/chemistry ; Polyethylene Glycols/chemical synthesis ; Polyethylene Glycols/chemistry ; Polyglactin 910/chemical synthesis ; Polyglactin 910/chemistry ; Polymerization ; Rheology
    Chemical Substances Biocompatible Materials ; Bone Cements ; Calcium Phosphates ; Polyesters ; poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer ; polyethylene glycol-poly(lactide-co-glycolide) ; Polyglactin 910 (34346-01-5) ; Polyethylene Glycols (3WJQ0SDW1A) ; tricalcium phosphate (K4C08XP666)
    Language English
    Publishing date 2019-01-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms20020391
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Functionalization Conditions of PLGA-PEG-PLGA Copolymer with Itaconic Anhydride

    Michlovská, Lenka / Vojtová, Lucy / Mravcová, Ludmila / Hermanová, Soňa / Kučerík, Jiří / Jančář, Josef

    Macromolecular symposia. 2010 Sept., v. 295, no. 1

    2010  

    Abstract: Biodegradable thermosensitive triblock copolymers based on poly(ethylene glycol) and poly(lactic-co-glycolic acid) (PLGA-PEG-PLGA) prepared via ring opening polymerization were modified by itaconic anhydride (ITA), which gives copolymer both reactive ... ...

    Abstract Biodegradable thermosensitive triblock copolymers based on poly(ethylene glycol) and poly(lactic-co-glycolic acid) (PLGA-PEG-PLGA) prepared via ring opening polymerization were modified by itaconic anhydride (ITA), which gives copolymer both reactive double bonds and functional carboxylic acid groups essential for the reaction with biological active material. Functionalization conditions comprising ITA purification, temperature, time and presence of solvent were optimized with the respect to amount of end-capped ITA. Maximum of 76.6 mol. % of bonded ITA were reached via "one pot" reaction in a bulk at 110 °C after 1.5 h. ITA functionalization thermally stabilized the original copolymer by increasing the initial degradation temperature Td from 284 °C to 294 °C and changing the negative glass transition temperature (Tg = -1.8 °C) to positive one up to 2.4 °C. The novel functionalized macromonomer can be cross-linked either chemically or physically in order to produce new functionalized hydrogel network applicable as biomedical material in tissue engineering.
    Language English
    Dates of publication 2010-09
    Size p. 119-124.
    Publishing place Wiley-VCH Verlag
    Document type Article
    ISSN 1022-1360
    DOI 10.1002/masy.200900071
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