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  1. Article ; Online: A widespread methylotroph acyl-homoserine lactone synthase produces a new quorum sensing signal that regulates swarming in Methylobacterium fujisawaense

    Mike Wallace / Dale A. Cummings, Jr. / Andrew G. Roberts / Aaron W. Puri

    mBio, Vol 15, Iss

    2024  Volume 1

    Abstract: ABSTRACTPink-pigmented facultative methylotrophs of the genera Methylorubrum and Methylobacterium are metabolically versatile bacteria that colonize many diverse environments on earth. Despite their widespread occurrence, the molecular details of how ... ...

    Abstract ABSTRACTPink-pigmented facultative methylotrophs of the genera Methylorubrum and Methylobacterium are metabolically versatile bacteria that colonize many diverse environments on earth. Despite their widespread occurrence, the molecular details of how these organisms interact with each other and their environment remain understudied. We analyzed genes encoding N-acylhomoserine lactone (acyl-HSL) quorum sensing signal synthases in the published genomes of these bacteria and determined that the product of the largest family of signal synthases had not been characterized. We identified 3R-OH-5Z-C12:1-HSL as a novel signal produced by these synthases using inverse stable isotopic labeling and structural characterization by mass spectrometry, nuclear magnetic resonance spectroscopy, and chemical synthesis. We show that in the methylotroph Methylobacterium fujisawaense DSM5686, this signal activates its cognate LuxR-family transcription factor and is produced in a positive feedback loop. We also discover that in this strain, quorum sensing negatively regulates swarming motility by activating the expression of a small protein that binds a predicted transcription factor. These results characterize a previously undescribed, yet widespread quorum sensing system used by pink-pigmented facultative methylotrophs, which helps us understand the chemical ecology of these important bacteria.IMPORTANCEBacteria known as pink-pigmented facultative methylotrophs colonize many diverse environments on earth, play an important role in the carbon cycle, and in some cases promote plant growth. However, little is known about how these organisms interact with each other and their environment. In this work, we identify one of the chemical signals commonly used by these bacteria and discover that this signal controls swarming motility in the pink-pigmented facultative methylotroph Methylobacterium fujisawaense DSM5686. This work provides new molecular details about interactions between these important bacteria and will help scientists predict ...
    Keywords methylotroph ; quorum sensing ; acyl-homoserine lactone ; chemical ecology ; swarming motility ; Microbiology ; QR1-502
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher American Society for Microbiology
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Viticultural terroirs in Stellenbosch, South Africa. I. The identification of natural terroir units

    Victoria Anne Carey / Dawid Saayman / Eben Archer / Gérard Barbeau / Mike Wallace

    OENO One, Vol 42, Iss 4, Pp 169-

    2008  Volume 183

    Abstract: Aims: A natural terroir unit (NTU) can be defined as a unit of land that is characterized by relatively homogenous topography, climate, geological substrate and soil. The mapping of NTUs is the first stage of data acquisition in a terroir study. This ... ...

    Abstract Aims: A natural terroir unit (NTU) can be defined as a unit of land that is characterized by relatively homogenous topography, climate, geological substrate and soil. The mapping of NTUs is the first stage of data acquisition in a terroir study. This study aimed to identify NTUs using a Geographic Information System and to characterize the Stellenbosch Wine of Origin District using existing digital information at the scale of a wine producing district. Methods and results: The study area is bordered by mountains, situated close to the Atlantic Ocean and bisected by the Eerste river valley, resulting in notable spatial variation of all climatic parameters. The geology is complex due to the high degree of tectonic movement and mixing of parent material. Terrain morphological units, altitude, aspect and soil type were used as primary keys/variables for the identification of NTUs. Each of the identified units was further described with respect to the extent of the expected sea breeze effect and, for certain of the soil types, the associated parent material. A total of 1389 NTUs were identified in the Stellenbosch Wine of Origin District. Conclusions: Many of the natural terroir units identified for the Stellenbosch Wine of Origin District are of a size that is not economically or practically viable. Significance and impact of the study: The natural terroir units should be grouped into larger, more manageable and thus more viable terroir units using data relating their viticultural and oenological potential. This task will be addressed in subsequent companion papers.
    Keywords geographic information system ; grapevine ; natural terroir unit ; Stellenbosch ; South Africa ; Agriculture ; S ; Botany ; QK1-989
    Subject code 550 ; 910
    Language English
    Publishing date 2008-12-01T00:00:00Z
    Publisher International Viticulture and Enology Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Viticultural terroirs in Stellenbosch, South Africa. I. The identification of natural terroir units

    Victoria Anne Carey / Dawid Saayman / Eben Archer / Gérard Barbeau / Mike Wallace

    OENO One. 2008 Dec., v. 42, no. 4

    2008  

    Abstract: Aims: A natural terroir unit (NTU) can be defined as a unit of land that is characterized by relatively homogenous topography, climate, geological substrate and soil. The mapping of NTUs is the first stage of data acquisition in a terroir study. This ... ...

    Abstract Aims: A natural terroir unit (NTU) can be defined as a unit of land that is characterized by relatively homogenous topography, climate, geological substrate and soil. The mapping of NTUs is the first stage of data acquisition in a terroir study. This study aimed to identify NTUs using a Geographic Information System and to characterize the Stellenbosch Wine of Origin District using existing digital information at the scale of a wine producing district. Methods and results: The study area is bordered by mountains, situated close to the Atlantic Ocean and bisected by the Eerste river valley, resulting in notable spatial variation of all climatic parameters. The geology is complex due to the high degree of tectonic movement and mixing of parent material. Terrain morphological units, altitude, aspect and soil type were used as primary keys/variables for the identification of NTUs. Each of the identified units was further described with respect to the extent of the expected sea breeze effect and, for certain of the soil types, the associated parent material. A total of 1389 NTUs were identified in the Stellenbosch Wine of Origin District. Conclusions: Many of the natural terroir units identified for the Stellenbosch Wine of Origin District are of a size that is not economically or practically viable. Significance and impact of the study: The natural terroir units should be grouped into larger, more manageable and thus more viable terroir units using data relating their viticultural and oenological potential. This task will be addressed in subsequent companion papers.
    Keywords altitude ; climate ; data collection ; geographic information systems ; landscapes ; river valleys ; soil types ; tectonics ; viticulture ; wines ; Atlantic Ocean ; South Africa
    Language English
    Dates of publication 2008-12
    Size p. 169-183.
    Publishing place International Viticulture and Enology Society
    Document type Article
    ISSN 2494-1271
    DOI 10.20870/oeno-one.2008.42.4.809
    Database NAL-Catalogue (AGRICOLA)

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