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  1. Article: [The process of bacterial population splitting into dissociants and long-term batch cultivation of bacteria].

    Mil'ko, E S / Mil'ko, D M

    Prikladnaia biokhimiia i mikrobiologiia

    2014  Volume 50, Issue 4, Page(s) 408–413

    Abstract: The growth and composition of a population were studied during long-term (up to 50 days) batch cultivation of mono and mixed cultures of Pseudomonas aeruginosa S- and M-dissociants and Rhodobacter sphaeroides R- and M-dissociants without the addition of ... ...

    Abstract The growth and composition of a population were studied during long-term (up to 50 days) batch cultivation of mono and mixed cultures of Pseudomonas aeruginosa S- and M-dissociants and Rhodobacter sphaeroides R- and M-dissociants without the addition of nutrients. During the cultivation of P. aeruginosa on a glucose-containing mineral medium, periodic lysis followed by polyculture growth resumption in the late stationary phase occurred on account of the M-dissociant: the change in its cell number corresponded to the change in the total cell number of the association. It was shown that the periodic occurrence of reducing sugars in the medium preceded the resumption ofpolyculture growth.
    MeSH term(s) Adaptation, Physiological ; Bacterial Load ; Batch Cell Culture Techniques ; Culture Media/chemistry ; Glucose/metabolism ; Microbial Viability ; Pseudomonas aeruginosa/growth & development ; Pseudomonas aeruginosa/metabolism ; Rhodobacter sphaeroides/growth & development ; Rhodobacter sphaeroides/metabolism ; Stress, Physiological ; Time Factors
    Chemical Substances Culture Media ; Glucose (IY9XDZ35W2)
    Language Russian
    Publishing date 2014-07
    Publishing country Russia (Federation)
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 412549-6
    ISSN 0555-1099
    ISSN 0555-1099
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: [Isolation of the photosynthetizing purple bacteria Rhodobacter sphaeroides dissociants and the study of their molecular, physiological-biochemical and morphological characteristics ].

    Ivanovskiĭ, R N / Mil'ko, E S / Mil'ko, D M

    Mikrobiologiia

    2013  Volume 82, Issue 2, Page(s) 169–175

    MeSH term(s) Light ; Polymerase Chain Reaction ; RNA, Ribosomal, 16S ; Rhodobacter sphaeroides/drug effects ; Rhodobacter sphaeroides/genetics ; Rhodobacter sphaeroides/physiology ; Rhodobacter sphaeroides/radiation effects ; Sodium Chloride/pharmacology ; Species Specificity ; Temperature ; Ultraviolet Rays
    Chemical Substances RNA, Ribosomal, 16S ; Sodium Chloride (451W47IQ8X)
    Language Russian
    Publishing date 2013-06-25
    Publishing country Russia (Federation)
    Document type Journal Article
    ZDB-ID 209707-2
    ISSN 0026-3656
    ISSN 0026-3656
    DOI 10.7868/s0026365613020067
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  3. Article: Poly-ß-hydroxybutyrate accumulation by Rhodobacter sphaeroides phase variants

    Krasil’nikova, E. N / Mil’ko, E. S / Keppen, O. I / Lebedeva, N. V / Ivanovskii, R. N

    Microbiology. 2015 May, v. 84, no. 3

    2015  

    Abstract: The R and M variants of a purple photosynthetic bacterium Rhodobacter sphaeroides 2R grown on a medium with acetate accumulate poly-ß-hydroxybutyrate (PHB). Accumulation of this polymer occurs in the cells grown either anaerobically on the light or ... ...

    Abstract The R and M variants of a purple photosynthetic bacterium Rhodobacter sphaeroides 2R grown on a medium with acetate accumulate poly-ß-hydroxybutyrate (PHB). Accumulation of this polymer occurs in the cells grown either anaerobically on the light or aerobically in the dark. On the medium with C/N imbalance (C/N = 4), PHB content during the stationary growth phase under aerobic conditions in the dark was 40 and 70% of the dry biomass of the R and M variant, respectively. The Rba. sphaeroides M variant is therefore a promising culture for large-scale PHB production. Investigation of activity of the TCA cycle enzymes revealed that decreased activity of citrate synthase, the key enzyme for acetate involvement in the reactions of the tricarboxylic acid cycle, was primarily responsible for enhanced PHB synthesis by Rba. sphaeroides. Moreover, the Rba. sphaeroides M variant grown under aerobic conditions in the dark exhibited considerably lower activity of NADH oxidase, which participates in the oxidation of reduced NADH produced in the TCA cycle during acetate oxidation. The combination of these two factors increases the possibilities for acetate assimilation via an alternative mechanism of PHB synthesis.
    Keywords NAD (coenzyme) ; Rhodobacter sphaeroides ; acetates ; aerobic conditions ; bacteria ; biomass ; citrate (si)-synthase ; oxidation ; polymers ; tricarboxylic acid cycle
    Language English
    Dates of publication 2015-05
    Size p. 347-350.
    Publishing place Pleiades Publishing
    Document type Article
    ZDB-ID 209706-0
    ISSN 1608-3237 ; 0026-2617
    ISSN (online) 1608-3237
    ISSN 0026-2617
    DOI 10.1134/S002626171503011X
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  4. Article: Isolation of the phase variants of the purple photosynthetic bacterium Rhodobacter sphaeroides and investigation of their molecular, physiological, biochemical, and morphological characteristics

    Ivanovskii, R. N / Mil’ko, E. S / Mil’ko, D. M

    Microbiology. 2013 Mar., v. 82, no. 2

    2013  

    Abstract: The ability of purple photosynthetic bacteria to form phase variants (dissociants) was discovered. The R and M phase variants were isolated from the Rhodobacter sphaeroides population, and their affiliation with the parent strain was confirmed by the PCR ...

    Abstract The ability of purple photosynthetic bacteria to form phase variants (dissociants) was discovered. The R and M phase variants were isolated from the Rhodobacter sphaeroides population, and their affiliation with the parent strain was confirmed by the PCR of their 16S rRNA gene fragments. The R and M variants were different both in colony morphology and in resistance to some physical and chemical factors. The R variant of Rhb. sphaeroides had advantages when grown in the light, at elevated temperature, under aeration, and UV irradiation, whereas the M variant had advantages when grown aerobically in the dark or at increased NaCl concentrations.
    Keywords Rhodobacter sphaeroides ; aeration ; genes ; mitosis ; photosynthetic bacteria ; polymerase chain reaction ; ribosomal RNA ; sodium chloride ; temperature ; ultraviolet radiation
    Language English
    Dates of publication 2013-03
    Size p. 169-174.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 209706-0
    ISSN 1608-3237 ; 0026-2617
    ISSN (online) 1608-3237
    ISSN 0026-2617
    DOI 10.1134/S0026261713020069
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  5. Article: Vyzhivaemost' dissotsiantov uglovodorodokisliaiushchego shtamma Pseudomonas aeruginosa pri khranenii.

    Mil'ko, E S

    Mikrobiologiia

    1998  Volume 67, Issue 1, Page(s) 102–105

    Abstract: The survival of R-, S-, and M-dissociants of the hydrocarbon-oxidizing strain Pseudomonas aeruginosa K-2 was compared after the storage of the cultures by four simple methods for 18 months. The best method was storage on slanted synthetic medium with ... ...

    Title translation Survival of dissociants of a hydrocarbon-oxidizing Pseudomonas aeruginosa strain during storage.
    Abstract The survival of R-, S-, and M-dissociants of the hydrocarbon-oxidizing strain Pseudomonas aeruginosa K-2 was compared after the storage of the cultures by four simple methods for 18 months. The best method was storage on slanted synthetic medium with hexadecane under mineral oil; under these conditions, the amount of viable cells of all dissociants showed the smallest decrease, and the composition of the stored populations underwent the least changes. During storage by other methods, the highest survival rate was demonstrated by R-cells, and M-cells were the most sensitive; this difference resulted in changes in the composition of stored populations. In stored populations of S-cells, which are the most active hydrocarbon oxidizers, the relative content of poorly active but more stable R-cells increased, which could result in a decrease in the hydrocarbon-degrading ability of the culture.
    MeSH term(s) Culture Media ; Hydrocarbons/metabolism ; Oxidation-Reduction ; Pseudomonas aeruginosa/metabolism ; Species Specificity ; Specimen Handling/methods ; Time Factors
    Chemical Substances Culture Media ; Hydrocarbons
    Language Russian
    Publishing date 1998-01
    Publishing country Russia (Federation)
    Document type English Abstract ; Journal Article
    ZDB-ID 209707-2
    ISSN 0026-3656
    ISSN 0026-3656
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  6. Article: Metabolism of the phase variants of the phototrophic bacterium Rhodobacter sphaeroides

    Mil’ko, E. S / Krasil’nikova, E. N / Keppen, O. I / Lebedeva, N. V / Ivanovsky, R. N

    Microbiology. 2014 July, v. 83, no. 4

    2014  

    Abstract: Growth, bacteriochlorophyll a content, electron transport chain (ETC), and activities of the tricarboxylic acid (TCA) cycle enzymes were studied in R and M phase variants of Rhodobacter sphaeroides cells grown anaerobically in the light and aerobically ... ...

    Abstract Growth, bacteriochlorophyll a content, electron transport chain (ETC), and activities of the tricarboxylic acid (TCA) cycle enzymes were studied in R and M phase variants of Rhodobacter sphaeroides cells grown anaerobically in the light and aerobically in the dark. Under all cultivation conditions tested, bacteriochlorophyll a content was 2–3 times lower in the cells of the M variant compared to the R variant, which therefore was predominant in the cultures grown in the light. In both variants, activity of all TCA cycle enzymes was higher for the cells grown in the dark under aerobic conditions. When grown aerobically in the dark, the R variant, unlike the M variant, did not contain cytochrome aa ₃, acting as cytochrome c oxidase, in its ETC. An additional point of coupling the electron transfer to the generation of the proton gradient at the cytochrome aa ₃ level provided for more efficient oxidation of organic substrates, resulting in predominance of the M variant in the cultures grown in the dark under aerobic conditions.
    Keywords Rhodobacter sphaeroides ; aerobic conditions ; cytochrome-c oxidase ; electron transfer ; electron transport chain ; mitosis ; oxidation ; photosynthetic bacteria ; tricarboxylic acid cycle
    Language English
    Dates of publication 2014-07
    Size p. 322-325.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 209706-0
    ISSN 1608-3237 ; 0026-2617
    ISSN (online) 1608-3237
    ISSN 0026-2617
    DOI 10.1134/S0026261714040122
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  7. Article: [Poly- β -Hydroxybutyrate Accumulation by Rhodobacter sphaeroides Phase Variants].

    Krasil'nikova, E N / Mil'ko, E S / Keppen, O I / Lebedeva, N V / Ivanovskii, R N

    Mikrobiologiia

    2015  Volume 84, Issue 3, Page(s) 311–315

    Abstract: The Rand M variants of a purple photosynthetic bacterium Rhodobacter sphaeroides 2R grown on a medium with acetate accumulate poly- β-hydroxybutyrate (PHB). Accumulation of this polymer occurs in the cells grown either anaerobically on the light or ... ...

    Abstract The Rand M variants of a purple photosynthetic bacterium Rhodobacter sphaeroides 2R grown on a medium with acetate accumulate poly- β-hydroxybutyrate (PHB). Accumulation of this polymer occurs in the cells grown either anaerobically on the light or aerobically in the dark. On the medium with C/N imbalance (C/N = 4), PHB content during the stationary growth phase under aerobic conditions in the dark was 40 and 70% of the dry biomass of the R and M variant, respectively. The Rba. sphaeroides M variant is therefore a promising culture for large-scale PHB production. Investigation of activity of the TCA cycle enzymes revealed that decreased activity of citrate synthase, the key enzyme for acetate involvement in the reactions of the tricarboxylic acids cycle, was primarily responsible for enhanced PHB synthesis by Rba. sphaeroides. Moreover, the Rba. sphaeroides M variant grown under aerobic conditions in the dark exhibited considerably lower activity of NADH oxidase, which participates in the oxidation of reduced NADH produced in the TCA cycle during acetate oxidation. The combination of these two factors increases the possibilities for acetate assimilation via an alternative mechanism of PHB synthesis.
    MeSH term(s) Acetates/metabolism ; Aerobiosis ; Anaerobiosis ; Citrate (si)-Synthase/metabolism ; Citric Acid Cycle ; Darkness ; Hydroxybutyrates/metabolism ; Light ; Multienzyme Complexes/metabolism ; NADH, NADPH Oxidoreductases/metabolism ; Oxidation-Reduction ; Polyesters/metabolism ; Rhodobacter sphaeroides/metabolism
    Chemical Substances Acetates ; Hydroxybutyrates ; Multienzyme Complexes ; Polyesters ; poly-beta-hydroxybutyrate (26063-00-3) ; NADH oxidase (EC 1.6.-) ; NADH, NADPH Oxidoreductases (EC 1.6.-) ; Citrate (si)-Synthase (EC 2.3.3.1)
    Language Russian
    Publishing date 2015-05
    Publishing country Russia (Federation)
    Document type English Abstract ; Journal Article
    ZDB-ID 209707-2
    ISSN 0026-3656
    ISSN 0026-3656
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: [Cultivation of Pseudomonas aeruginosa dissociants under specified limitation conditions].

    Fursova, P V / Mil'ko, E S / Levich, A P

    Mikrobiologiia

    2008  Volume 77, Issue 2, Page(s) 226–231

    Abstract: The possibility of controlling the development of microbial communities was investigated on the basis of experimentally determined requirements for basic nutrients in R, S, and M dissociants of Pseudomonas aeruginosa. On media with the limitation ... ...

    Abstract The possibility of controlling the development of microbial communities was investigated on the basis of experimentally determined requirements for basic nutrients in R, S, and M dissociants of Pseudomonas aeruginosa. On media with the limitation conditions preset on the basis of the predictions of a mathematical model, exhaustion of glucose was experimentally confirmed for all monocultures and mixed cultures, as well as balanced consumption of glucose, nitrogen, and phosphorus by the R dissociant at the corresponding initial medium composition. The experimentally determined composition of mixed cultures was found to conform to the ones calculated using the mathematical model. The data obtained suggest the possibility of cyclic consumption of phosphorus by P. aeruginosa.
    MeSH term(s) Culture Media ; Glucose/metabolism ; Models, Theoretical ; Nitrogen/metabolism ; Phosphorus/metabolism ; Pseudomonas aeruginosa/physiology
    Chemical Substances Culture Media ; Phosphorus (27YLU75U4W) ; Glucose (IY9XDZ35W2) ; Nitrogen (N762921K75)
    Language Russian
    Publishing date 2008-03
    Publishing country Russia (Federation)
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 209707-2
    ISSN 0026-3656
    ISSN 0026-3656
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  9. Article: [metabolism of the phase variants of the phototrophic bacterium Rhodobacter sphaeroides].

    Mil'ko, E S / Krasil'nikova, E N / Keppen, O I / Lebedeva, N V / Ivanovskiĭ, R N

    Mikrobiologiia

    2014  Volume 83, Issue 4, Page(s) 411–415

    Abstract: Growth, bacteriochlorophyll a content, electron transport chain (ETC), and activities of the tricarboxylic acid (TCA) cycle enzymes were studied in R and M phase variants of Rhodobactersphaeroides cells grown anaerobically in the light and aerobically in ...

    Abstract Growth, bacteriochlorophyll a content, electron transport chain (ETC), and activities of the tricarboxylic acid (TCA) cycle enzymes were studied in R and M phase variants of Rhodobactersphaeroides cells grown anaerobically in the light and aerobically in the dark. Under all cultivation conditions tested, bacteriochlorophyll a content was 2-3 times lower in the cells of the M. variant compared to the R variant, which therefore was predominant in the cultures grown in the light. In both variants, activity of all TCA cycle enzymes was higher for the cells grown in the dark under aerobic conditions. When grown aerobically in the dark, the R variant, unlike the M variant, did not contain cytochrome aa3, acting as. cytochrome c oxidase, in its ETC. An additional point of coupling the electron transfer to the generation of the proton gradient al the cytochrome aa3 level provided for more efficient oxidation of organic substrates, resulting in predominance of the M variant in the cultures grown in the dark under aerobic conditions.
    MeSH term(s) Bacterial Proteins/metabolism ; Bacteriochlorophyll A/metabolism ; Citric Acid Cycle/physiology ; Electron Transport/physiology ; Electron Transport Complex IV/metabolism ; Proton-Motive Force/physiology ; Rhodobacter sphaeroides/metabolism
    Chemical Substances Bacterial Proteins ; Bacteriochlorophyll A ; bacteriochlorophyll A proteins, Bacteria ; Electron Transport Complex IV (EC 1.9.3.1)
    Language Russian
    Publishing date 2014-07
    Publishing country Russia (Federation)
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 209707-2
    ISSN 0026-3656
    ISSN 0026-3656
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Growth peculiarities of hydrocarbon-oxidizing rhodococcus and pseudomonads dissociates in mono- and mixed cultures

    Mil'ko, E. S / Maksimovich, M. O / Lopatina, L. I / Porodenko, E. V

    Applied biochemistry and microbiology. 2010 Sept., v. 46, no. 5

    2010  

    Abstract: Growth of R-, S-, and M-dissociates of Rhodococcus rubropertinctus in mixed culture with R-, S-, and M-dissociates of Rhodococcus aeruginosa in comparison with rhodococcus monoculture cultivated on mineral nutrient medium with hexadecane has been studied. ...

    Abstract Growth of R-, S-, and M-dissociates of Rhodococcus rubropertinctus in mixed culture with R-, S-, and M-dissociates of Rhodococcus aeruginosa in comparison with rhodococcus monoculture cultivated on mineral nutrient medium with hexadecane has been studied. The amount of cells in the stationary growth phase has increased 10-15 times in comparison with the monoculture, and pseudomonads which dominated in population, in associations of M-dissociate of R. rubropertinctus with any dissociate of R. aeruginosa or two S-dissociates in the studied bacterial species. The emulsifying ability of the cells (maximal in R-dissociates), the synthesis of surface active compounds in pseudomonads, which is maximal in M-dissociate had the main influence on the growth of rhodococci on the nutrient medium with hydrocarbons.
    Keywords Gordonia rubripertinctus ; hexadecane ; mixed culture
    Language English
    Dates of publication 2010-09
    Size p. 492-496.
    Publisher SP MAIK Nauka/Interperiodica
    Publishing place Dordrecht
    Document type Article
    ZDB-ID 412550-2
    ISSN 1608-3024 ; 0003-6838
    ISSN (online) 1608-3024
    ISSN 0003-6838
    DOI 10.1134/S0003683810050054
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