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  1. Article ; Online: Evolved distal tail protein of skunaviruses facilitates adsorption to exopolysaccharide-encoding lactococci.

    Millen, Anne M / Magill, Damian / Romero, Dennis / Simdon, Laura

    Microbiome research reports

    2023  Volume 2, Issue 4, Page(s) 26

    Abstract: Aim: ...

    Abstract Aim:
    Language English
    Publishing date 2023-07-18
    Publishing country United States
    Document type Journal Article
    ISSN 2771-5965
    ISSN (online) 2771-5965
    DOI 10.20517/mrr.2023.29
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Host-encoded, cell surface-associated exopolysaccharide required for adsorption and infection by lactococcal P335 phage subtypes.

    Millen, Anne M / Romero, Dennis A / Horvath, Philippe / Magill, Damian / Simdon, Laura

    Frontiers in microbiology

    2022  Volume 13, Page(s) 971166

    Abstract: Lactococcus ... ...

    Abstract Lactococcus lactis
    Language English
    Publishing date 2022-10-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.971166
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Genetic determinants of lactococcal C2viruses for host infection and their role in phage evolution.

    Millen, Anne M / Romero, Dennis A

    The Journal of general virology

    2016  Volume 97, Issue 8, Page(s) 1998–2007

    Abstract: Lactococcus lactis is an industrial starter culture used for the production of fermented dairy products. Pip (phage infection protein) bacteriophage-insensitive mutant (BIM) L. lactis DGCC11032 was isolated following challenge of parental strain DGCC7271 ...

    Abstract Lactococcus lactis is an industrial starter culture used for the production of fermented dairy products. Pip (phage infection protein) bacteriophage-insensitive mutant (BIM) L. lactis DGCC11032 was isolated following challenge of parental strain DGCC7271 with C2viruses. Over a period of industrial use, phages infecting DGCC11032 were isolated from industrial whey samples and identified as C2viruses. Although Pip is reported to be the receptor for many C2viruses including species type phage c2, a similar cell-membrane-associated protein, YjaE, was recently reported as the receptor for C2virus bIL67. Characterization of DGCC7271 BIMs following challenge with phage capable of infecting DGCC11032 identified mutations in yjaE, confirming YjaE to be necessary for infection. DGCC7271 YjaE mutants remained sensitive to the phages used to generate pip variant DGCC11032, indicating a distinction in host phage determinants. We will refer to C2viruses requiring Pip as c2-type andC2viruses that require YjaE as bIL67-type. Genomic comparisons of two c2-type phages unable to infect pip mutant DGCC11032 and four bIL67-type phages isolated on DGCC11032 confirmed the segregation of each group based on resemblance to prototypical phages c2 and bIL67, respectively. The distinguishing feature is linked to three contiguous late-expressed genes: l14-15-16 (c2) and ORF34-35-36 (bIL67). Phage recombinants in which the c2-like l14-15-16 homologue gene set was exchanged with corresponding bIL67 genes ORF34-35-36 were capable of infecting a pip mutated host. Together, these results correlate the phage genes corresponding to l14-15-16 (c2) and ORF34-35-36 (bIL67) to host lactococcal phage determinants Pip and YjaE, respectively.
    MeSH term(s) Bacteriophages/genetics ; Bacteriophages/growth & development ; Genes, Viral ; Genome, Viral ; Lactococcus lactis/virology ; Receptors, Virus ; Sequence Analysis, DNA ; Virus Attachment
    Chemical Substances Receptors, Virus
    Language English
    Publishing date 2016-07-07
    Publishing country England
    Document type Comparative Study ; Journal Article
    ZDB-ID 219316-4
    ISSN 1465-2099 ; 0022-1317
    ISSN (online) 1465-2099
    ISSN 0022-1317
    DOI 10.1099/jgv.0.000499
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  4. Article ; Online: Lactococcus lactis type III-A CRISPR-Cas system cleaves bacteriophage RNA.

    Millen, Anne M / Samson, Julie E / Tremblay, Denise M / Magadán, Alfonso H / Rousseau, Geneviève M / Moineau, Sylvain / Romero, Dennis A

    RNA biology

    2018  Volume 16, Issue 4, Page(s) 461–468

    Abstract: CRISPR-Cas defends microbial cells against invading nucleic acids including viral genomes. Recent studies have shown that type III-A CRISPR-Cas systems target both RNA and DNA in a transcription-dependent manner. We previously found a type III-A system ... ...

    Abstract CRISPR-Cas defends microbial cells against invading nucleic acids including viral genomes. Recent studies have shown that type III-A CRISPR-Cas systems target both RNA and DNA in a transcription-dependent manner. We previously found a type III-A system on a conjugative plasmid in Lactococcus lactis which provided resistance against virulent phages of the Siphoviridae family. Its naturally occurring spacers are oriented to generate crRNAs complementary to target phage mRNA, suggesting transcription-dependent targeting. Here, we show that only constructs whose spacers produce crRNAs complementary to the phage mRNA confer phage resistance in L. lactis. In vivo nucleic acid cleavage assays showed that cleavage of phage dsDNA genome was not detected within phage-infected L. lactis cells. On the other hand, Northern blots indicated that the lactococcal CRISPR-Cas cleaves phage mRNA in vivo. These results cannot exclude that single-stranded phage DNA is not being targeted, but phage DNA replication has been shown to be impaired.
    MeSH term(s) Base Sequence ; CRISPR-Cas Systems/genetics ; DNA, Intergenic/genetics ; DNA, Viral/genetics ; Lactococcus lactis/genetics ; RNA, Viral/genetics ; Virus Replication/genetics
    Chemical Substances DNA, Intergenic ; DNA, Viral ; RNA, Viral
    Language English
    Publishing date 2018-10-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1555-8584
    ISSN (online) 1555-8584
    DOI 10.1080/15476286.2018.1502589
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Mobile CRISPR/Cas-mediated bacteriophage resistance in Lactococcus lactis.

    Millen, Anne M / Horvath, Philippe / Boyaval, Patrick / Romero, Dennis A

    PloS one

    2012  Volume 7, Issue 12, Page(s) e51663

    Abstract: Lactococcus lactis is a biotechnological workhorse for food fermentations and potentially therapeutic products and is therefore widely consumed by humans. It is predominantly used as a starter microbe for fermented dairy products, and specialized strains ...

    Abstract Lactococcus lactis is a biotechnological workhorse for food fermentations and potentially therapeutic products and is therefore widely consumed by humans. It is predominantly used as a starter microbe for fermented dairy products, and specialized strains have adapted from a plant environment through reductive evolution and horizontal gene transfer as evidenced by the association of adventitious traits with mobile elements. Specifically, L. lactis has armed itself with a myriad of plasmid-encoded bacteriophage defensive systems to protect against viral predation. This known arsenal had not included CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins), which forms a remarkable microbial immunity system against invading DNA. Although CRISPR/Cas systems are common in the genomes of closely related lactic acid bacteria (LAB), none was identified within the eight published lactococcal genomes. Furthermore, a PCR-based search of the common LAB CRISPR/Cas systems (Types I and II) in 383 industrial L. lactis strains proved unsuccessful. Here we describe a novel, Type III, self-transmissible, plasmid-encoded, phage-interfering CRISPR/Cas discovered in L. lactis. The native CRISPR spacers confer resistance based on sequence identity to corresponding lactococcal phage. The interference is directed at phages problematic to the dairy industry, indicative of a responsive system. Moreover, targeting could be modified by engineering the spacer content. The 62.8-kb plasmid was shown to be conjugally transferrable to various strains. Its mobility should facilitate dissemination within microbial communities and provide a readily applicable system to naturally introduce CRISPR/Cas to industrially relevant strains for enhanced phage resistance and prevention against acquisition of undesirable genes.
    MeSH term(s) Bacteriophages/genetics ; Bacteriophages/pathogenicity ; Dairy Products/microbiology ; Fermentation ; Inverted Repeat Sequences/genetics ; Inverted Repeat Sequences/immunology ; Lactococcus lactis/genetics ; Lactococcus lactis/immunology ; Lactococcus lactis/virology ; Plasmids/genetics ; Plasmids/immunology
    Language English
    Publishing date 2012-12-11
    Publishing country United States
    Document type Journal Article
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0051663
    Database MEDical Literature Analysis and Retrieval System OnLINE

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