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Article ; Online: Patch Track Software for Measuring Kinematic Phenotypes of Arabidopsis Roots Demonstrated on Auxin Transport Mutants.

Henry, Ashley R / Miller, Nathan D / Spalding, Edgar P

International journal of molecular sciences

2023 Volume 24, Issue 22

Abstract: Plant roots elongate when cells produced in the apical meristem enter a transient period of rapid expansion. To measure the dynamic process of root cell expansion in the elongation zone, we captured digital images of growing Arabidopsis roots with ... ...

Abstract	Plant roots elongate when cells produced in the apical meristem enter a transient period of rapid expansion. To measure the dynamic process of root cell expansion in the elongation zone, we captured digital images of growing Arabidopsis roots with horizontal microscopes and analyzed them with a custom image analysis program (PatchTrack) designed to track the growth-driven displacement of many closely spaced image patches. Fitting a flexible logistics equation to patch velocities plotted versus position along the root axis produced the length of the elongation zone (mm), peak relative elemental growth rate (% h
MeSH term(s)	Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Biomechanical Phenomena ; Plant Roots/metabolism ; Membrane Transport Proteins/metabolism ; Indoleacetic Acids ; Software ; Gene Expression Regulation, Plant
Chemical Substances	Arabidopsis Proteins ; Membrane Transport Proteins ; Indoleacetic Acids
Language	English
Publishing date	2023-11-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms242216475
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Article ; Online: A quantum Hopfield associative memory implemented on an actual quantum processor.

Miller, Nathan Eli / Mukhopadhyay, Saibal

Scientific reports

2021 Volume 11, Issue 1, Page(s) 23391

Abstract: In this work, we present a Quantum Hopfield Associative Memory (QHAM) and demonstrate its capabilities in simulation and hardware using IBM Quantum Experience.. The QHAM is based on a quantum neuron design which can be utilized for many different machine ...

Abstract	In this work, we present a Quantum Hopfield Associative Memory (QHAM) and demonstrate its capabilities in simulation and hardware using IBM Quantum Experience.. The QHAM is based on a quantum neuron design which can be utilized for many different machine learning applications and can be implemented on real quantum hardware without requiring mid-circuit measurement or reset operations. We analyze the accuracy of the neuron and the full QHAM considering hardware errors via simulation with hardware noise models as well as with implementation on the 15-qubit ibmq_16_melbourne device. The quantum neuron and the QHAM are shown to be resilient to noise and require low qubit overhead and gate complexity. We benchmark the QHAM by testing its effective memory capacity and demonstrate its capabilities in the NISQ-era of quantum hardware. This demonstration of the first functional QHAM to be implemented in NISQ-era quantum hardware is a significant step in machine learning at the leading edge of quantum computing.
Language	English
Publishing date	2021-12-03
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-02866-z
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Article ; Online: BRXL4‐LAZY1 interaction at the plasma membrane controls Arabidopsis branch angle and gravitropism

Che, Ximing / Splitt, Bessie L. / Eckholm, Magnus T. / Miller, Nathan D. / Spalding, Edgar P.

The Plant Journal. 2023 Jan., v. 113, no. 2 p.211-224

2023

Abstract: Gravitropism guides growth to shape plant architecture above and below ground. Mutations in LAZY1 impair stem gravitropism and cause less upright inflorescence branches (wider angles). The LAZY1 protein resides at the plasma membrane and in the nucleus. ... ...

Abstract	Gravitropism guides growth to shape plant architecture above and below ground. Mutations in LAZY1 impair stem gravitropism and cause less upright inflorescence branches (wider angles). The LAZY1 protein resides at the plasma membrane and in the nucleus. The plasma membrane pool is necessary and sufficient for setting branch angles. To investigate the molecular mechanism of LAZY1 function, we screened for LAZY1‐interacting proteins in yeast. We identified BRXL4, a shoot‐specific protein related to BREVIS RADIX. The BRXL4‐LAZY1 interaction occurred at the plasma membrane in plant cells, and not detectably in the nucleus. Mutations in the C‐terminus of LAZY1, but not other conserved regions, prevented the interaction. Opposite to lazy1, brxl4 mutants displayed faster gravitropism and more upright branches. Overexpressing BRXL4 produced strong lazy1 phenotypes. The apparent negative regulation of LAZY1 function is consistent with BRXL4 reducing LAZY1 expression or the amount of LAZY1 at the plasma membrane. Measurements indicated that both are true. LAZY1 mRNA was three‐fold more abundant in brxl4 mutants and almost undetectable in BRXL4 overexpressors. Plasma membrane LAZY1 was higher and nuclear LAZY1 lower in brxl4 mutants compared with the wild type. To explain these results, we suggest that BRXL4 reduces the amount of LAZY1 at the plasma membrane where it functions in gravity signaling and promotes LAZY1 accumulation in the nucleus where it reduces LAZY1 expression, possibly by suppressing its own transcription. This explanation of how BRXL4 negatively regulates LAZY1 suggests ways to modify shoot system architecture for practical purposes.
Keywords	Arabidopsis ; gravitropism ; gravity ; plant architecture ; plasma membrane ; yeasts
Language	English
Dates of publication	2023-01
Size	p. 211-224.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	1088037-9
ISSN	1365-313X ; 0960-7412
ISSN (online)	1365-313X
ISSN	0960-7412
DOI	10.1111/tpj.16055
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Article: Technique for Reconstruction of Midcarpal Instability Associated with Lunotriquetral Coalition.

Hall, Shannon M / Lee, Frank G / Wallace, Sean J / Miller, Nathan F

Plastic and reconstructive surgery. Global open

2022 Volume 10, Issue 7, Page(s) e4430

Abstract: Carpal coalition is a rare congenital presentation of 2 or more fused carpal bones due to a failure of apoptotic segmentation during development. The most common subtype is lunotriquetral coalition (LTC). Most cases are asymptomatic and found ... ...

Abstract	Carpal coalition is a rare congenital presentation of 2 or more fused carpal bones due to a failure of apoptotic segmentation during development. The most common subtype is lunotriquetral coalition (LTC). Most cases are asymptomatic and found incidentally on imaging; however, a few symptomatic cases requiring treatment have been reported. Surgical intervention of arthrodesis and proximal row carpectomy in adults have been reported where conservative management of splinting, physiotherapy, anti-inflammatory medication, or steroid injections have failed. We report a unique case of Minnaar type 2 LTC in a 20-year-old man with a 6-year history of daily right wrist pain and symptomatic wrist instability whose previous conservative therapies failed. Midcarpal instability and volar intercalated segment instability-volar flexion of the lunate-were present. A novel technique using the palmaris longus tendon to reconstruct the triquetrohamate, triquetrocapitate, and dorsal radiolunate ligaments was performed. The graft was secured dorsally to the hamate, triquetrum, and capitate. An additional graft from the lunate to distal radius acted as a biomechanical checkrein. There were no complications. Temporary Kirschner wires were removed 2 months postoperatively, followed by occupational hand therapy. At 1-year follow-up, the patient no longer reported pain or lunotriquetral tenderness. Midcarpal instability and volar intercalated segment instability resolved. Postoperative right wrist flexion and extension were 40 and 75 degrees, respectively. We discuss the successful outcome of this novel technique as an alternative to arthrodesis in the surgical management of LTC.
Language	English
Publishing date	2022-07-07
Publishing country	United States
Document type	Case Reports
ZDB-ID	2851682-5
ISSN	2169-7574 ; 2169-7574
ISSN (online)	2169-7574
ISSN	2169-7574
DOI	10.1097/GOX.0000000000004430
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Article ; Online: Complications with the Zurich Canine Total Hip Replacement System in an Initial Series of Cases Performed by a Single Surgeon.

Franklin, Samuel P / Miller, Nathan A / Riecks, Todd

Veterinary and comparative orthopaedics and traumatology : V.C.O.T

2021 Volume 34, Issue 5, Page(s) 346–351

Abstract: Objective: The aim of this study was to quantify the complications using the Zurich total hip replacement system in an initial series of cases performed by a single surgeon who had experience with other total hip replacement systems.: Materials and ... ...

Abstract	Objective: The aim of this study was to quantify the complications using the Zurich total hip replacement system in an initial series of cases performed by a single surgeon who had experience with other total hip replacement systems. Materials and methods: This was a retrospective study in which complications were classified as major if any treatment was needed or if the outcome was less than near-normal function. Complications that did not warrant treatment and that did not result in function that was inferior to near-normal were considered minor. Outcomes were assessed by radiographic review, physical examination, subjective gait evaluation or, in one case, by objective gait analysis. Bilateral total hip replacements were considered separate procedures. Results: The first 21 procedures in 19 dogs performed by a single surgeon were included. The mean time to follow-up was 48 weeks (range: 8-120 weeks; standard deviation: 36 weeks). Two cases (of 21) experienced major complications including one dog with excess internal femoral rotation during weight bearing and one dog having luxation. One case (of 21) had a minor complication; femoral fracture in the presence of an intact bone plate that maintained alignment and healed without treatment. Clinical significance: A high rate of successful outcomes with few major complications can be obtained in the initial cases treated using the Zurich total hip replacement system for surgeons with prior experience with other total hip replacement systems.
MeSH term(s)	Animals ; Arthroplasty, Replacement, Hip/adverse effects ; Arthroplasty, Replacement, Hip/veterinary ; Dog Diseases/diagnostic imaging ; Dog Diseases/etiology ; Dog Diseases/surgery ; Dogs ; Humans ; Postoperative Complications/epidemiology ; Postoperative Complications/etiology ; Postoperative Complications/veterinary ; Retrospective Studies ; Surgeons ; Treatment Outcome
Language	English
Publishing date	2021-06-03
Publishing country	Germany
Document type	Journal Article
ZDB-ID	286750-3
ISSN	2567-6911 ; 0932-0814
ISSN (online)	2567-6911
ISSN	0932-0814
DOI	10.1055/s-0041-1730366
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Book ; Online: A Quantum Hopfield Associative Memory Implemented on an Actual Quantum Processor

Miller, Nathan Eli / Mukhopadhyay, Saibal

2021

Abstract: In this work, we present a Quantum Hopfield Associative Memory (QHAM) and demonstrate its capabilities in simulation and hardware using IBM Quantum Experience. The QHAM is based on a quantum neuron design which can be utilized for many different machine ... ...

Abstract	In this work, we present a Quantum Hopfield Associative Memory (QHAM) and demonstrate its capabilities in simulation and hardware using IBM Quantum Experience. The QHAM is based on a quantum neuron design which can be utilized for many different machine learning applications and can be implemented on real quantum hardware without requiring mid-circuit measurement or reset operations. We analyze the accuracy of the neuron and the full QHAM considering hardware errors via simulation with hardware noise models as well as with implementation on the 15-qubit ibmq_16_melbourne device. The quantum neuron and the QHAM are shown to be resilient to noise and require low qubit overhead and gate complexity. We benchmark the QHAM by testing its effective memory capacity and demonstrate its capabilities in the NISQ-era of quantum hardware. This demonstration of the first functional QHAM to be implemented in NISQ-era quantum hardware is a significant step in machine learning at the leading edge of quantum computing. Comment: 16 pages, 10 figures, 3 tables. The manuscript has been revised to improve clarity of the manuscript text, mathematics and overall argument. The presented results are unchanged
Keywords	Quantum Physics ; Computer Science - Machine Learning
Publishing date	2021-05-24
Publishing country	us
Document type	Book ; Online
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Article ; Online: Archaeological and modern whitemouth croaker fish (Micropogonias furnieri) of southeastern Brazil: A geochemical proxy for environmental preference

Lopes, Mariana Samor / Aguilera, Orangel / Monteiro-Neto, Cassiano / Miller, Nathan R. / Gaspar, Maria Dulce / Costa, Marcus Rodrigues da

The Holocene. 2023 July, v. 33, no. 7 p.890-902

2023

Abstract: In this study, laser ablation–inductively coupled plasma–mass spectrometry was used to characterize the growth patterns of otolith of Micropogonias furnieri from southeastern Brazil under the influence of coastal marine upwelling. Archaeological fish ... ...

Abstract	In this study, laser ablation–inductively coupled plasma–mass spectrometry was used to characterize the growth patterns of otolith of Micropogonias furnieri from southeastern Brazil under the influence of coastal marine upwelling. Archaeological fish sagittae otoliths of M. furnieri recovered from Brazilian shell mounds (5820‒4980 cal BP) were analysed and compared with those of modern specimens. Otolith Sr/Ca analysis indicated that off southeastern Brazil, M. furnieri shifted from freshwater/brackish in archaeological specimens to brackish/marine in modern times. Regular core-to-edge Sr/Ca oscillations suggest that both modern and archaeological M. furnieri are resident estuarine fish that also use the marine waters adjacent to the coastal zone. Based on analyses of a wide range of otolith edge Sr/Ca values measured in archaeological samples, pre-colonial fishing activities in the coastal regions of Guanabara Bay occurred throughout the year. This study reinforces the importance of M. furnieri as a precolonial fishing resource in this area and demonstrates a shift in fishing practices, where smaller fish are targetted within the bay and larger fish are caught beyond the bay. These data may also be indicative of a shift in the life cycle of this species, possibly caused by the overfishing of this important resource and significant increase in human activities in protected coastal waters in modern times.
Keywords	Holocene epoch ; Micropogonias furnieri ; archaeology ; coasts ; estuarine fish ; freshwater ; humans ; otoliths ; spectroscopy ; Brazil ; habitat preference ; Holocene ; micro-chemistry ; resource management ; sagittae otolith ; shell mounds
Language	English
Dates of publication	2023-07
Size	p. 890-902.
Publishing place	SAGE Publications
Document type	Article ; Online
ZDB-ID	2027956-5
ISSN	1477-0911 ; 0959-6836
ISSN (online)	1477-0911
ISSN	0959-6836
DOI	10.1177/09596836231163510
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Article: Double Spiral Dupuytren's Disease: Case of Ulnar and Radial Spiral Cords in Shared Digit.

Kator, Jamie / Gopinath, Rohan / Miller, Nathan / Pensy, Raymond

Plastic and reconstructive surgery. Global open

2020 Volume 8, Issue 10, Page(s) e3212

Abstract: Dupuytren's disease is a benign fibroproliferative disorder resulting in progressive contraction of palmar and digital fascia. Diseased fascia of the hand condenses into tensile cords, manifesting in various anatomical combinations. The spiral cord ... ...

Abstract	Dupuytren's disease is a benign fibroproliferative disorder resulting in progressive contraction of palmar and digital fascia. Diseased fascia of the hand condenses into tensile cords, manifesting in various anatomical combinations. The spiral cord variant is especially troublesome, as the cord travels under the digital neurovascular bundle and places it at risk during surgical resection. In severe cases, limited fasciectomy is required to release contracture and restore finger extension. Here, we present the case of a 79-year-old right-handed man with a history of severe Dupuytren's disease of both hands, who was found to have
Language	English
Publishing date	2020-10-28
Publishing country	United States
Document type	Case Reports
ZDB-ID	2851682-5
ISSN	2169-7574 ; 2169-7574
ISSN (online)	2169-7574
ISSN	2169-7574
DOI	10.1097/GOX.0000000000003212
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Article: Conservative Management of Median Nerve Brachial Plexopathy after Microwave-based MiraDry Treatment for Axillary Hyperhidrosis.

Lee, Frank G / Mansour, Ahmed M / Wallace, Sean J / Miller, Nathan F

Plastic and reconstructive surgery. Global open

2021 Volume 9, Issue 12, Page(s) e3992

Abstract: Axillary hyperhidrosis is characterized by excessive sweating of the armpits, which can significantly affect quality of life. A new microwave-based therapy, MiraDry (Miramar labs, Sunnyvale, Calif.), is a promising minimally-invasive treatment option. We ...

Abstract	Axillary hyperhidrosis is characterized by excessive sweating of the armpits, which can significantly affect quality of life. A new microwave-based therapy, MiraDry (Miramar labs, Sunnyvale, Calif.), is a promising minimally-invasive treatment option. We report a case of unilateral brachial plexus thermal injury in a thin 19-year-old man treated for axillary hyperhidrosis with the MiraDry system. He initially experienced swelling and pain in the left hand and was prescribed 1 week of methylprednisolone. He then presented 1 week later with induration and swelling of bilateral axillae with swelling of left thumb, left index, and left long fingers, decreased sensation in median nerve distribution of the left hand, and the inability to flex the left index finger DIP joint. EMG showed absent median nerve motor and sensory function, consistent with median nerve plexopathy. He was conservatively managed with close observation and regular occupational hand therapy appointments. At his 12-month follow-up, there was complete return of left pronator teres strength, thumb flexion, and index finger flexion. Decreased sensation remained at the tip of the left index finger. We report the case of median nerve palsy after MiraDry therapy for axillary hyperhidrosis in a thin young man. We recommend using low-energy settings and pre-procedural ultrasound for young, thin patients because of the more superficial course of the brachial plexus within the axilla.
Language	English
Publishing date	2021-12-15
Publishing country	United States
Document type	Case Reports
ZDB-ID	2851682-5
ISSN	2169-7574 ; 2169-7574
ISSN (online)	2169-7574
ISSN	2169-7574
DOI	10.1097/GOX.0000000000003992
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Article ; Online: Comparison of osteotomy level with three different canine total hip replacement systems.

Franklin, Samuel P / Franklin, Ashley L / Feyerabend, Nathan / Hayashi, Kei / Miller, Nathan A / Cook, James L

Open veterinary journal

2022 Volume 12, Issue 6, Page(s) 975–979

Abstract: Background: Numerous cementless total hip replacement (THR) systems are available for application in dogs and one of the potential differences among these systems is the technique for performing a femoral osteotomy and the amount of bone preserved in ... ...

Abstract	Background: Numerous cementless total hip replacement (THR) systems are available for application in dogs and one of the potential differences among these systems is the technique for performing a femoral osteotomy and the amount of bone preserved in the calcar region. However, no quantitative comparison of osteotomy level has been performed for canine THRs to date. Aims: To develop and validate a method for quantifying the level of the osteotomy at its most distomedial aspect in conjunction with canine THR and to compare osteotomy level between multiple different THRs. Methods: Immediate post-operative cranial-caudal or caudal-cranial radiographs of 33 dogs treated with 17 Helica and 17 BFX THR were assessed and osteotomy level was quantified using a novel radiographic assessment by 3 independent observers. Correlation among observers was quantified using a Spearman rank order correlation. Osteotomy location was subsequently quantified for an additional 10 Zurich THRs. The osteotomy level for each THR was subsequently compared between Helica, BFX, and Zurich THRs using one-way non-parametric Mann-Whitney rank sum tests and significance set at Results: R Conclusion: The strong correlations among three different observers indicate that the technique for measuring the location of the distomedial aspect of the osteotomy was acceptably precise. The osteotomies made in conjunction with the short-stemmed Helica implants were significantly more proximal than those made with both of the long-stemmed (BFX and Zurich) femoral prostheses. The distomedial aspect of the osteotomy with the BFX system was significantly more proximal than that with the Zurich THR, indicating that between these two long-stemmed systems the osteotomy level is unique.
MeSH term(s)	Dogs ; Animals ; Arthroplasty, Replacement, Hip/veterinary ; Arthroplasty, Replacement, Hip/methods ; Dog Diseases/diagnostic imaging ; Dog Diseases/surgery ; Osteotomy/veterinary ; Radiography ; Postoperative Period
Language	English
Publishing date	2022-12-13
Publishing country	Libya
Document type	Journal Article
ZDB-ID	2651664-0
ISSN	2218-6050 ; 2218-6050
ISSN (online)	2218-6050
ISSN	2218-6050
DOI	10.5455/OVJ.2022.v12.i6.25
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