LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Zurück zur letzten Suche Suchhistorie
Erweiterte Suche

Ihre letzten Suchen

  1. AU="Mills, Mary Katherine"
  2. AU="Vajente, G."
  3. AU="Bhatnagar, R.C"
  4. AU="Prasad Sarkale"
  5. AU="Manfredonia, Laura"
  6. AU="Linssen, L"
  7. AU="Davide, Borroni"
  8. AU="Ingrid M. Wentzensen"
  9. AU="A.Parida, "
  10. AU="Zhu, D H"
  11. AU=Ulloa Luis
  12. AU="Böhme, Elisa"
  13. AU=Trko?lu Oya
  14. AU="Levine, Zoe"
  15. AU="Banaszkiewicz, Paul A."
  16. AU="Datrier, Laurence E. H."
  17. AU=Fala Loretta
  18. AU="McGuckin, M M"
  19. AU="Winlaw, David S"
  20. AU="Gökmen, M Refik"
  21. AU="Islam, Tousif"
  22. AU="Szczepanczyk, Marek J"
  23. AU="Boregowda, Siddaraju"
  24. AU="Lomidzew, D."

Suchergebnis

Treffer 1 - 6 von insgesamt 6

Suchoptionen

  1. Artikel ; Online: microRNA Expression Dynamics in Culicoides sonorensis Biting Midges Following Blood-Feeding

    Mills, Mary Katherine / Rozo-Lopez, Paula / Bryant, William Bart / Drolet, Barbara S.

    Insects. 2023 July 06, v. 14, no. 7

    2023  

    Abstract: Culicoides sonorensis midges vector multiple livestock arboviruses, resulting in significant economic losses worldwide. Due to the tight association between virus transmission, blood feeding, and egg development, understanding midge physiology is ... ...

    Abstract Culicoides sonorensis midges vector multiple livestock arboviruses, resulting in significant economic losses worldwide. Due to the tight association between virus transmission, blood feeding, and egg development, understanding midge physiology is paramount to limiting pathogen transmission. Previous studies have demonstrated the importance of small non-coding RNAs (ncRNAs), specifically microRNAs (miRNAs), in multiple aspects of vector physiology. These small ncRNAs regulate gene expression at the post-transcriptional level and display differential expression during pathogen infection. Due to the lack of annotated miRNAs in the biting midge and associated expression profiles, we used small RNA-Seq and miRDeep2 analyses to determine the Culicoides miRNAs in whole females and midgut tissues in response to blood feeding. Our analyses revealed 76 miRNAs within C. sonorensis composed of 73 orthologous and three candidate novel miRNAs, as well as conserved miRNA clusters. miRNA conservation suggests an interesting evolutionary relationship between miRNA expression and hematophagy in the infraorder Culicomorpha. We also identified multiple blood meal-regulated and tissue-enriched miRNAs. Lastly, we further identified miRNAs with expression patterns potentially associated with virus infection by probing publicly available datasets. Together, our data provide a foundation for future ncRNA work to untangle the dynamics of gene regulation associated with midge physiology.
    Schlagwörter Culicoides sonorensis ; arboviruses ; blood ; data collection ; eggs ; gene expression ; gene expression regulation ; hematophagy ; livestock ; microRNA ; midges ; midgut ; pathogens ; physiology ; sequence analysis ; virus transmission
    Sprache Englisch
    Erscheinungsverlauf 2023-0706
    Erscheinungsort Multidisciplinary Digital Publishing Institute
    Dokumenttyp Artikel ; Online
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects14070611
    Datenquelle NAL Katalog (AGRICOLA)

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  2. Artikel: microRNA Expression Dynamics in

    Mills, Mary Katherine / Rozo-Lopez, Paula / Bryant, William Bart / Drolet, Barbara S

    Insects

    2023  Band 14, Heft 7

    Abstract: Culicoides ... ...

    Abstract Culicoides sonorensis
    Sprache Englisch
    Erscheinungsdatum 2023-07-06
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects14070611
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  3. Artikel: Global Analysis of Small Non-Coding RNA Populations across Tissues in the Malaria Vector,

    Bryant, William Bart / Ray, Savanna / Mills, Mary Katherine

    Insects

    2020  Band 11, Heft 7

    Abstract: Malaria is a major global health problem, where the anautogenous female ... ...

    Abstract Malaria is a major global health problem, where the anautogenous female mosquito
    Sprache Englisch
    Erscheinungsdatum 2020-06-30
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects11070406
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  4. Artikel ; Online: Small RNA-Seq Analysis Reveals miRNA Expression Dynamics Across Tissues in the Malaria Vector,

    Bryant, William Bart / Mills, Mary Katherine / Olson, Bradley Jsc / Michel, Kristin

    G3 (Bethesda, Md.)

    2019  Band 9, Heft 5, Seite(n) 1507–1517

    Abstract: Malaria continues to be a major global health problem, where disease transmission is deeply linked to the repeated blood feeding nature of the anautogenous mosquito. Given the tight link between blood feeding and disease transmission, understanding basic ...

    Abstract Malaria continues to be a major global health problem, where disease transmission is deeply linked to the repeated blood feeding nature of the anautogenous mosquito. Given the tight link between blood feeding and disease transmission, understanding basic biology behind mosquito physiology is a requirement for developing effective vector-borne disease control strategies. In the mosquito, numerous loss of function studies with notable phenotypes demonstrate microRNAs (miRNAs) play significant roles in mosquito physiology. While the field appreciates the importance of a handful of miRNAs, we still need global mosquito tissue miRNA transcriptome studies. To address this need, our goal was to determine the miRNA transcriptome for multiple tissues of the pre-vitellogenic mosquito. To this end, by using small RNA-Seq analysis, we determined miRNA transcriptomes in tissues critical for mosquito reproduction and immunity including (i) fat body-abdominal wall enriched tissues, (ii) midguts, (iii) ovaries, and (iv) remaining tissues comprised of the head and thorax. We found numerous examples of miRNAs exhibiting pan-tissue high- or low- expression, tissue exclusion, and tissue enrichment. We also updated and consolidated the miRNA catalog and provided a detailed genome architecture map for the malaria vector,
    Mesh-Begriff(e) Animals ; Anopheles/genetics ; Gene Expression Profiling ; Gene Expression Regulation ; Malaria/parasitology ; Malaria/transmission ; MicroRNAs/genetics ; Mosquito Vectors/genetics ; Organ Specificity ; Sequence Analysis, RNA ; Transcriptome
    Chemische Substanzen MicroRNAs
    Sprache Englisch
    Erscheinungsdatum 2019-05-07
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2629978-1
    ISSN 2160-1836 ; 2160-1836
    ISSN (online) 2160-1836
    ISSN 2160-1836
    DOI 10.1534/g3.119.400104
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  5. Artikel ; Online: CRISPR-Cas9 Long-Read Sequencing for Mapping Transgenes in the Mouse Genome.

    Bryant, W Bart / Yang, Allison / Griffin, Susan H / Zhang, Wei / Rafiq, Ashiq M / Han, Weiping / Deak, Ferenc / Mills, Mary Katherine / Long, Xiaochun / Miano, Joseph M

    The CRISPR journal

    2023  Band 6, Heft 2, Seite(n) 163–175

    Abstract: Microinjected transgenes, both large and small, are known to insert randomly into the mouse genome. Traditional methods of mapping a transgene are challenging, thus complicating breeding strategies and accurate interpretation of phenotypes, particularly ... ...

    Abstract Microinjected transgenes, both large and small, are known to insert randomly into the mouse genome. Traditional methods of mapping a transgene are challenging, thus complicating breeding strategies and accurate interpretation of phenotypes, particularly when a transgene disrupts critical coding or noncoding sequences. As the vast majority of transgenic mouse lines remain unmapped, we developed CRISPR-Cas9 Long-Read Sequencing (CRISPR-LRS) to ascertain transgene integration loci. This novel approach mapped a wide size range of transgenes and uncovered more complex transgene-induced host genome re-arrangements than previously appreciated. CRISPR-LRS offers a facile, informative approach to establish robust breeding practices and will enable researchers to study a gene without confounding genetic issues. Finally, CRISPR-LRS will find utility in rapidly and accurately interrogating gene/genome editing fidelity in experimental and clinical settings.
    Mesh-Begriff(e) Animals ; Mice ; Gene Editing ; CRISPR-Cas Systems/genetics ; Transgenes ; Genome/genetics ; Mice, Transgenic
    Sprache Englisch
    Erscheinungsdatum 2023-04-18
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 3017891-5
    ISSN 2573-1602 ; 2573-1599
    ISSN (online) 2573-1602
    ISSN 2573-1599
    DOI 10.1089/crispr.2022.0099
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 7195: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 119: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  6. Artikel ; Online: Pseudomonas aeruginosa in Musca domestica L.: temporospatial examination of bacteria population dynamics and house fly antimicrobial responses.

    Joyner, Chester / Mills, Mary Katherine / Nayduch, Dana

    PloS one

    2013  Band 8, Heft 11, Seite(n) e79224

    Abstract: House flies associate with microbes throughout their life history. Bacteria ingested by adult flies enter the alimentary canal and face a hostile environment including antimicrobial defenses. Because the outcome of this interaction impacts bacterial ... ...

    Abstract House flies associate with microbes throughout their life history. Bacteria ingested by adult flies enter the alimentary canal and face a hostile environment including antimicrobial defenses. Because the outcome of this interaction impacts bacterial survival and dissemination, our primary objective was to understand the temporospatial dynamics of fly-bacteria associations. We concurrently examined the temporospatial fate of GFP-expressing Pseudomonas aeruginosa (GFP-P. aeruginosa) in the house fly alimentary canal along with antimicrobial peptide (AMP) expression. Motile, viable GFP-P. aeruginosa were found in all regions of the alimentary canal and were culturable throughout the observation period (2-24 h). A significant decrease in recoverable bacteria occurred between 2 and 12 h, followed by an increase between 12 and 24 h. qRT-PCR analysis showed expression of the AMPs cecropin, diptericin, and defensin both locally (gut) and systemically. Furthermore, mRNA of all AMPs were expressed throughout gut tissues, with some tissue-specific temporal variation. Interestingly, fluctuation in recoverable P. aeruginosa was associated with AMP protein expression in the gut (immunofluorescent signal detection), but not with mRNA (qRTPCR). In regards to vector competence, flies excreted GFP-P. aeruginosa throughout the 24 h period, serving as both reservoirs and disseminators of this bacterium. Collectively, our data show flies can harbor and disseminate P. aeruginosa, and that the interactions of fly defenses with bacteria can influence vector competence.
    Mesh-Begriff(e) Animals ; Gastrointestinal Tract/immunology ; Gastrointestinal Tract/microbiology ; Houseflies/immunology ; Houseflies/microbiology ; Insect Proteins/immunology ; Pseudomonas Infections/immunology ; Pseudomonas aeruginosa/immunology ; RNA, Messenger/immunology
    Chemische Substanzen Insect Proteins ; RNA, Messenger
    Sprache Englisch
    Erscheinungsdatum 2013-11-18
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0079224
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Zum Seitenanfang