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Article ; Online: Passive Immunotherapies Targeting Amyloid-

Marković, Milica / Milošević, Jelica / Wang, Weirong / Cao, Yanguang

2023 Volume 105, Issue 1, Page(s) 1–13

Abstract: Alzheimer's disease (AD) is a neurodegenerative disorder characterized by amyloid- ...

Abstract	Alzheimer's disease (AD) is a neurodegenerative disorder characterized by amyloid-
MeSH term(s)	Humans ; Alzheimer Disease/drug therapy ; Network Pharmacology ; Amyloid beta-Peptides/chemistry ; Amyloid beta-Peptides/metabolism ; Antibodies, Monoclonal/pharmacology ; Antibodies, Monoclonal/therapeutic use ; Immunization, Passive ; Immunotherapy/methods
Chemical Substances	donanemab ; Amyloid beta-Peptides ; Antibodies, Monoclonal
Language	English
Publishing date	2023-12-15
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	124034-1
ISSN	1521-0111 ; 0026-895X
ISSN (online)	1521-0111
ISSN	0026-895X
DOI	10.1124/molpharm.123.000726
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Article ; Online: Structural Transitions of Papain-like Cysteine Proteases: Implications for Sensor Development.

Marković, Srdjan / Andrejević, Natalija S / Milošević, Jelica / Polović, Natalija Đ

Biomimetics (Basel, Switzerland)

2023 Volume 8, Issue 3

Abstract: The significant role of papain-like cysteine proteases, including papain, cathepsin L and SARS-CoV-2 PLpro, in biomedicine and biotechnology makes them interesting model systems for sensor development. These enzymes have a free thiol group that is ... ...

Abstract	The significant role of papain-like cysteine proteases, including papain, cathepsin L and SARS-CoV-2 PLpro, in biomedicine and biotechnology makes them interesting model systems for sensor development. These enzymes have a free thiol group that is suitable for many sensor designs including strong binding to gold nanoparticles or low-molecular-weight inhibitors. Focusing on the importance of the preservation of native protein structure for inhibitor-binding and molecular-imprinting, which has been applied in some efficient examples of sensor development, the aim of this work was to examine the effects of the free-thiol-group's reversible blocking on papain denaturation that is the basis of its activity loss and aggregation. To utilize biophysical methods common in protein structural transitions characterization, such as fluorimetry and high-resolution infrared spectroscopy, low-molecular-weight electrophilic thiol blocking reagent S-Methyl methanethiosulfonate (MMTS) was used in solution. MMTS binding led to a two-fold increase in 8-Anilinonaphthalene-1-sulfonic acid fluorescence, indicating increased hydrophobic residue exposure. A more in-depth analysis showed significant transitions on the secondary structure level upon MMTS binding, mostly characterized by the lowered content of α-helices and unordered structures (either for approximately one third), and the increase in aggregation-specific β-sheets (from 25 to 52%) in a dose-dependant manner. The recovery of this inhibited protein showed that reversibility of inhibition is accompanied by reversibility of protein denaturation. Nevertheless, a 100-fold molar excess of the inhibitor led to the incomplete recovery of proteolytic activity, which can be explained by irreversible denaturation. The structural stability of the C-terminal β-sheet rich domain of the papain-like cysteine protease family opens up an interesting possibility to use its foldamers as a strategy for sensor development and other multiple potential applications that rely on the great commercial value of papain-like cysteine proteases.
Language	English
Publishing date	2023-07-01
Publishing country	Switzerland
Document type	Journal Article
ISSN	2313-7673
ISSN (online)	2313-7673
DOI	10.3390/biomimetics8030281
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Article ; Online: On the Protein Fibrillation Pathway: Oligomer Intermediates Detection Using ATR-FTIR Spectroscopy.

Milošević, Jelica / Prodanović, Radivoje / Polović, Natalija

Molecules (Basel, Switzerland)

2021 Volume 26, Issue 4

Abstract: Oligomeric intermediates on the pathway of amyloid fibrillation are suspected as the main cytotoxins responsible for amyloid-related pathogenicity. As they appear to be a part of the lag phase of amyloid fibrillation when analyzed using standard methods ... ...

Abstract	Oligomeric intermediates on the pathway of amyloid fibrillation are suspected as the main cytotoxins responsible for amyloid-related pathogenicity. As they appear to be a part of the lag phase of amyloid fibrillation when analyzed using standard methods such as Thioflavin T (ThT) fluorescence, a more sensitive method is needed for their detection. Here we apply Fourier transform infrared spectroscopy (FTIR) in attenuated total reflectance (ATR) mode for fast and cheap analysis of destabilized hen-egg-white lysozyme solution and detection of oligomer intermediates of amyloid fibrillation. Standard methods of protein aggregation analysis- Thioflavin T (ThT) fluorescence, atomic force microscopy (AFM), and 8-anilinonaphthalene-1-sulphonic acid (ANS) fluorescence were applied and compared to FTIR spectroscopy data. Results show the great potential of FTIR for both, qualitative and quantitative monitoring of oligomer formation based on the secondary structure changes. While oligomer intermediates do not induce significant changes in ThT fluorescence, their secondary structure changes were very prominent. Normalization of specific Amide I region peak intensities by using Amide II peak intensity as an internal standard provides an opportunity to use FTIR spectroscopy for both qualitative and quantitative analysis of biological samples and detection of potentially toxic oligomers, as well as for screening of efficiency of fibrillation procedures.
MeSH term(s)	Amyloid/chemistry ; Amyloid/ultrastructure ; Amyloidogenic Proteins/chemistry ; Amyloidogenic Proteins/ultrastructure ; Animals ; Benzothiazoles/chemistry ; Chickens ; Fluorescence ; Microscopy, Atomic Force ; Muramidase/chemistry ; Muramidase/ultrastructure ; Protein Structure, Secondary ; Spectroscopy, Fourier Transform Infrared
Chemical Substances	Amyloid ; Amyloidogenic Proteins ; Benzothiazoles ; thioflavin T (2390-54-7) ; hen egg lysozyme (EC 3.2.1.-) ; Muramidase (EC 3.2.1.17)
Language	English
Publishing date	2021-02-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules26040970
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Article ; Online: Potential Protective Role of Galectin-3 in Patients with Gonarthrosis and Diabetes Mellitus: A Cross-Sectional Study.

Milosevic, Jelena / Jurisevic, Milena / Grbovic, Vesna / Jovanovic, Ivan / Gajovic, Nevena / Jurisic-Skevin, Aleksandra

International journal of environmental research and public health

2022 Volume 19, Issue 18

Abstract: Background: Gonarthrosis and diabetes mellitus are two diseases that are increasingly being linked. The aim of this study was to quantify serum levels of Gal-3, pro- and anti-inflammatory cytokines (including their ratios and correlations), and ... ...

Abstract	Background: Gonarthrosis and diabetes mellitus are two diseases that are increasingly being linked. The aim of this study was to quantify serum levels of Gal-3, pro- and anti-inflammatory cytokines (including their ratios and correlations), and participant's condition (pain, stiffness, functional limitations) in gonarthrosis patients with and without diabetes mellitus. Methods: A between-subject, cross-sectional experimental design was adopted. Serum levels of TNF-α, IL-6, IL-12, IL-23, IFN-γ, IL-17, IL-10, Gal-3, and WOMAC score were measured. Results: Gonarthrosis patients with diabetes mellitus had significantly ( Conclusions: Correlation between Gal-3 and proinflammatory cytokines and its dominance over proinflammatory cytokines implicate the potential role of Gal-3 in preventing cartilage destruction.
MeSH term(s)	Cross-Sectional Studies ; Cytokines ; Diabetes Mellitus ; Galectin 3/metabolism ; Humans ; Interleukin-10 ; Interleukin-12 ; Interleukin-17 ; Interleukin-23 ; Interleukin-6 ; Tumor Necrosis Factor-alpha
Chemical Substances	Cytokines ; Galectin 3 ; Interleukin-17 ; Interleukin-23 ; Interleukin-6 ; Tumor Necrosis Factor-alpha ; Interleukin-10 (130068-27-8) ; Interleukin-12 (187348-17-0)
Language	English
Publishing date	2022-09-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph191811480
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Article ; Online: Diagnosis and treatment of angiographically unclear coronary lesions.

Maričić, Bojan / Perišić, Zoran / Kostić, Tomislav / Božinović, Nenad / Apostolović, Svetlana / Šalinger, Sonja / Maričić, Tijana / Vasilev, Vladimir / Juričić, Stefan / Dakić, Sonja / Perišić, Jelena / Milošević, Jelena

The Journal of international medical research

2024 Volume 52, Issue 1, Page(s) 3000605231213753

Abstract: Although selective coronary angiography is the gold standard diagnostic technique for coronary lesions, this method does not provide all information regarding pathophysiologic mechanisms. We herein describe a patient in their early 60s with a 3-month ... ...

Abstract	Although selective coronary angiography is the gold standard diagnostic technique for coronary lesions, this method does not provide all information regarding pathophysiologic mechanisms. We herein describe a patient in their early 60s with a 3-month history of pronounced angina. Coronary angiography revealed a central line of illumination in the proximomedial segment of the right coronary artery, suggesting a chronic coronary dissection/recanalized thrombus, along with positive remodeling and TIMI grade 2 flow. Optical coherence tomography showed a recanalized thrombus and multiple lumens separated by thin septa. Because of the significantly reduced flow and signs of ischemia in the right coronary artery irrigation territory, we decided to perform percutaneous coronary intervention. Post-treatment optical coherence tomography indicated optimal apposition and expansion of the stents with positive remodeling of the blood vessel. We believe that decisions regarding treatment modalities should be guided by the presence or absence of ischemia. Lesions that are causing myocardial ischemia should be revascularized; otherwise, medical treatment can be utilized.
MeSH term(s)	Humans ; Drug-Eluting Stents ; Coronary Artery Disease/diagnosis ; Coronary Angiography ; Coronary Vessels/diagnostic imaging ; Coronary Vessels/surgery ; Coronary Vessels/pathology ; Tomography, Optical Coherence ; Percutaneous Coronary Intervention ; Thrombosis/pathology ; Ischemia ; Treatment Outcome
Language	English
Publishing date	2024-01-05
Publishing country	England
Document type	Journal Article
ZDB-ID	184023-x
ISSN	1473-2300 ; 0300-0605 ; 0142-2596
ISSN (online)	1473-2300
ISSN	0300-0605 ; 0142-2596
DOI	10.1177/03000605231213753
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Zs.A 925: Show issues

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Article ; Online: Comparative stability of ficin and papain in acidic conditions and the presence of ethanol.

Milošević, Jelica / Janković, Brankica / Prodanović, Radivoje / Polović, Natalija

Amino acids

2019 Volume 51, Issue 5, Page(s) 829–838

Abstract: Proteolytic enzymes are used for proteolysis and peptide synthesis which can be run in various conditions including low pH value and the presence of ethanol. The most common cysteine protease applied in acidic-alcoholic conditions is well-characterized ... ...

Abstract	Proteolytic enzymes are used for proteolysis and peptide synthesis which can be run in various conditions including low pH value and the presence of ethanol. The most common cysteine protease applied in acidic-alcoholic conditions is well-characterized papain. Ficin, which is closely related to papain in terms of proteolytic activity and substrate specificity, could potentially be applied in the alcoholic beverage industry and peptide synthesis. The aim of this study was to compare papain and ficin stability in process conditions. Comparative stability study showed that ficin as a mixture of different isoforms has a broader range of stability in respect of pH and cold storage stability, in comparison to papain. It retains about 70% of initial activity after 3-week cold storage at low pH and in the presence of ethanol. Unlike ficin, papain loses about 70% of initial activity in the same incubation period as it is more prone to non-native aggregation that was confirmed by FTIR analysis. The presence of multiple isoforms of ficin stabilizes the protease against cold denaturation and aggregation, making it more suitable for biotechnological and laboratory usage than single papain isoform. It is more cold-stable in alcoholic-acidic and acidic conditions suggesting possible replacement of papain with even lower enzyme concentration.
MeSH term(s)	Acids/chemistry ; Ethanol/chemistry ; Ficain/chemistry ; Humans ; Hydrogen-Ion Concentration ; Kinetics ; Papain/chemistry ; Protein Stability
Chemical Substances	Acids ; Ethanol (3K9958V90M) ; Papain (EC 3.4.22.2) ; Ficain (EC 3.4.22.3)
Language	English
Publishing date	2019-03-19
Publishing country	Austria
Document type	Comparative Study ; Journal Article
ZDB-ID	1121341-3
ISSN	1438-2199 ; 0939-4451
ISSN (online)	1438-2199
ISSN	0939-4451
DOI	10.1007/s00726-019-02724-3
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Article: Spontaneous coronary artery dissection in women in the generative period: clinical characteristics, treatment, and outcome-a systematic review and meta-analysis.

Apostolović, Svetlana / Ignjatović, Aleksandra / Stanojević, Dragana / Radojković, Danijela Djordjević / Nikolić, Miroslav / Milošević, Jelena / Filipović, Tamara / Kostić, Katarina / Miljković, Ivana / Djoković, Aleksandra / Krljanac, Gordana / Mehmedbegović, Zlatko / Ilić, Ivan / Aleksandrić, Srdjan / Paradies, Valeria

Frontiers in cardiovascular medicine

2024 Volume 11, Page(s) 1277604

Abstract: Introduction: Spontaneous coronary artery dissection (SCAD) is a non-traumatic and non-iatrogenic separation of the coronary arterial wall.: Materials and methods: This systematic review and meta-analysis is reported following the PRISMA guidelines ... ...

Abstract	Introduction: Spontaneous coronary artery dissection (SCAD) is a non-traumatic and non-iatrogenic separation of the coronary arterial wall. Materials and methods: This systematic review and meta-analysis is reported following the PRISMA guidelines and is registered in the PROSPERO database. A literature search was focused on female patients in generative period (16-55 of age) with acute coronary syndrome (ACS) caused by SCAD, and comparison from that database NP-SCAD (spontaneous coronary artery dissection in non pregnant women) and P-SCAD (spontaneous coronary artery dissection in pregnant women). Results: 14 studies with 2,145 females in the generative period with ACS caused by SCAD were analyzed. The median age was 41 years (33.4-52.3 years). The most common risk factor was previous smoking history in 24.9% cases. The most common clinical presentation of ACS was STEMI in 47.4%. Conservative treatment was reported in 41.1%. PCI was performed in 32.7%, and 3.8% of patients had CABG surgery. LAD was the most frequently affected (50.5%). The prevalence of composite clinical outcomes including mortality, non-fatal MI and recurrent SCAD was 3.3% (95% CI: 1.4-5.1), 37.7% (95% CI: 1.9-73.4) and 15.2% (95% CI: 9.1-21.3) of patients. P-SCAD compared to NP-SCAD patients more frequently had STEMI (OR = 3.16; 95% CI: 2.30-4.34; Conclusion: The results of this meta-analysis indicated that patients with P-SCAD more frequently had STEMI, and events more frequently involved left main and LAD compared to NP-SCAD patients. Women with NP-SCAD were significantly more often treated conservatively compared to P-SCAD patients. P-SCAD compared to NP-SCAD patients did not have significantly higher mortality rates or recurrent coronary dissection.
Language	English
Publishing date	2024-02-08
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2781496-8
ISSN	2297-055X
ISSN	2297-055X
DOI	10.3389/fcvm.2024.1277604
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Article: Comparative stability of ficin and papain in acidic conditions and the presence of ethanol

Milošević, Jelica / Janković, Brankica / Polović, Natalija / Prodanović, Radivoje

Amino acids. 2019 May, v. 51, no. 5

2019

Abstract: Proteolytic enzymes are used for proteolysis and peptide synthesis which can be run in various conditions including low pH value and the presence of ethanol. The most common cysteine protease applied in acidic–alcoholic conditions is well-characterized ... ...

Abstract	Proteolytic enzymes are used for proteolysis and peptide synthesis which can be run in various conditions including low pH value and the presence of ethanol. The most common cysteine protease applied in acidic–alcoholic conditions is well-characterized papain. Ficin, which is closely related to papain in terms of proteolytic activity and substrate specificity, could potentially be applied in the alcoholic beverage industry and peptide synthesis. The aim of this study was to compare papain and ficin stability in process conditions. Comparative stability study showed that ficin as a mixture of different isoforms has a broader range of stability in respect of pH and cold storage stability, in comparison to papain. It retains about 70% of initial activity after 3-week cold storage at low pH and in the presence of ethanol. Unlike ficin, papain loses about 70% of initial activity in the same incubation period as it is more prone to non-native aggregation that was confirmed by FTIR analysis. The presence of multiple isoforms of ficin stabilizes the protease against cold denaturation and aggregation, making it more suitable for biotechnological and laboratory usage than single papain isoform. It is more cold-stable in alcoholic–acidic and acidic conditions suggesting possible replacement of papain with even lower enzyme concentration.
Keywords	alcoholic beverages ; beverage industry ; cold ; cold storage ; denaturation ; ethanol ; ficain ; Fourier transform infrared spectroscopy ; papain ; peptides ; pH ; proteolysis ; storage quality ; substrate specificity
Language	English
Dates of publication	2019-05
Size	p. 829-838.
Publishing place	Springer Vienna
Document type	Article
ZDB-ID	1121341-3
ISSN	1438-2199 ; 0939-4451
ISSN (online)	1438-2199
ISSN	0939-4451
DOI	10.1007/s00726-019-02724-3
Database	NAL-Catalogue (AGRICOLA)

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Zs.A 3490: Show issues

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Article: An Ultrafast One-Step Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Detection of SARS-CoV-2.

Milosevic, Jadranka / Lu, Mengrou / Greene, Wallace / He, Hong-Zhang / Zheng, Si-Yang

Frontiers in microbiology

2021 Volume 12, Page(s) 749783

Abstract: We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on ... ...

Abstract	We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop
Language	English
Publishing date	2021-11-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2021.749783
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Article ; Online: Predictors of poor neonatal outcomes in fetuses diagnosed with congenital urinary tract anomalie.

Pop-Trajkovic Dinic, Sonja / Zivadinovic, Radomir / Stefanovic, Milan / Trenkic, Milan / Milosevic, Jelena / Mitic, Dejan

Ginekologia polska

2021

Abstract: Objectives: Urinary tract anomalies account for approximately one-quarter of all antenatally detected anomalies. The aim of this study was to identify factors associated with severe adverse neonatal outcomes of a prenatally diagnosed urinary tract ... ...

Abstract	Objectives: Urinary tract anomalies account for approximately one-quarter of all antenatally detected anomalies. The aim of this study was to identify factors associated with severe adverse neonatal outcomes of a prenatally diagnosed urinary tract anomaly. Material and methods: A retrospective-prospective study included 101 pregnant women with prenatally diagnosed fetal urinary tract anomalies presented to the Council for Fetal Anomalies. Prenatal diagnoses were compared with autopsy findings in cases of terminated pregnancy or with clinical and operative findings of the infants. Results: The mortality rate in the group of patients with fetal obstructive uropathy (60 patients) was 10% and in the group of patients with fetal multicystic dysplastic kidney (38 patients) 15.7%. Surgery was performed on 53.4% of the children, whereas more than half of the operations involved resolving associated urinary tract anomalies. Postoperative renal function deterioration occurred in 19% of the children. Conclusions: The prognosis of renal function in obstructive uropathies is excellent if oligoamnios does not develop prenatally and in case of timely provided surgical care is provided postnatally. The finding of the bilateral multicystic dysplastic kidney is associated with poor prognosis. The prognosis in fetal unilateral multicystic dysplastic kidney depends primarily on the condition of the contralateral kidney and the existence of associated anomalies.
Language	English
Publishing date	2021-04-12
Publishing country	Poland
Document type	Journal Article
ZDB-ID	130894-4
ISSN	2543-6767 ; 0017-0011
ISSN (online)	2543-6767
ISSN	0017-0011
DOI	10.5603/GP.a2021.0032
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