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  1. Article ; Online: Characterization of Enterococcus hirae Isolated from the Intestine of Seabass (Lates Calcarifer) as a New Potential Probiotic against Pathogenic Vibrios.

    Masduki, Fathiah / Y, Jasmin M / Min, Chong Chou / Karim, Murni

    Current microbiology

    2020  Volume 77, Issue 12, Page(s) 3962–3968

    Abstract: In this study, we aimed to isolate, identify and characterize lactic acid bacteria (LAB) from the intestine of juvenile seabass (Lates calcarifer) as a new potential probiotic. Four strains of LABs were isolated from the intestines of ten healthy seabass ...

    Abstract In this study, we aimed to isolate, identify and characterize lactic acid bacteria (LAB) from the intestine of juvenile seabass (Lates calcarifer) as a new potential probiotic. Four strains of LABs were isolated from the intestines of ten healthy seabass juveniles. In the in vitro screening process using spot lawn assay, one isolate labeled as LAB3 showed inhibitory activity against Vibrio harveyi (ATCC 35,084). Strain LAB3 was determined to belong to the gram positive bacteria group with cocci shape and was identified as Enterococcus hirae using 16S rDNA analysis. This bacterium was able to grow at pH ranging from pH 2 to 10 with the best growth at pH 7. This strain was also able to grow at 0-4% NaCl after 24 h incubation and grew best at 1.5% NaCl. Enterococcus hirae strain LAB3 of the present study is worthy to be further characterized as a potential probiotic for use in seabass culture.
    MeSH term(s) Animals ; Bass ; Enterococcus hirae ; Intestines ; Probiotics ; Vibrio
    Language English
    Publishing date 2020-10-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 134238-1
    ISSN 1432-0991 ; 0343-8651
    ISSN (online) 1432-0991
    ISSN 0343-8651
    DOI 10.1007/s00284-020-02228-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1446: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Immunomodulatory effects of Isochrysis galbana incorporated diet on Oreochromis sp. (red hybrid tilapia) via Sera-

    Ahamad Bustamam, Muhammad Safwan / Pantami, Hamza Ahmed / Shaari, Khozirah / Min, Chong Chou / Mediani, Ahmed / Ismail, Intan Safinar

    Fish & shellfish immunology

    2022  Volume 132, Page(s) 108455

    Abstract: Tilapia is one of the most common fish species that is intensively produced all over the world. However, significant measures at improving aquaculture health must be taken since disease outbreaks are often encountered in the rapidly developing ... ...

    Abstract Tilapia is one of the most common fish species that is intensively produced all over the world. However, significant measures at improving aquaculture health must be taken since disease outbreaks are often encountered in the rapidly developing aquaculture industry. Therefore, the objective of the study was designed to evaluate the metabolite changes in tilapia' sera through
    Language English
    Publishing date 2022-12-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 1067738-0
    ISSN 1095-9947 ; 1050-4648
    ISSN (online) 1095-9947
    ISSN 1050-4648
    DOI 10.1016/j.fsi.2022.108455
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 5917: Show issues

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  3. Article ; Online: α‐2‐macroglobulin, an infection‐resistant biomarker in Epinephelus fuscoguttatus (Forsskål, 1775)

    Ibrahim, Nur Liyana / Webb, Conner / Nazarudin, Muhammad Farhan / Min, Chong Chou / Din, Mohamed Shariff Mohamed / Robinson, Colin / Abdullah, Maha

    Aquaculture Research. 2022 Nov., v. 53, no. 16 p.5618-5627

    2022  

    Abstract: Epinephelus fuscoguttatus (Forsskål), or brown‐marbled grouper, is a favoured species among aquaculture farmers for its relative easy management and high commercial value. Disease outbreaks, however, negatively impact farmers' livelihoods by reducing ... ...

    Abstract Epinephelus fuscoguttatus (Forsskål), or brown‐marbled grouper, is a favoured species among aquaculture farmers for its relative easy management and high commercial value. Disease outbreaks, however, negatively impact farmers' livelihoods by reducing fish harvest yields. Traditional selective breeding of disease‐resistant strains is still being practised due to limitations in vaccines and effective antibiotics. Development of biomarkers and molecular quantitative techniques has potential to advance approaches in handling outbreaks of disease. This study aimed to determine selected proteins' expression patterns and suitability as infection‐resistant markers in brown‐marbled grouper. Experimental infection of brown‐marbled grouper with Vibrio parahaemolyticus was established. Fingerlings (N = 70) were acclimatized and maintained in saltwater at 28 ± 2°C, pH 7.9 ± 1, salinity 29 ± 1 ppt and dissolved oxygen 5 ppm. V. parahaemolyticus at 5 × 10¹¹ CFU/ml was injected into the dorsal area of the fish and monitored for 7 days. Expressions of alpha‐2‐macroglobulin, nattectin and parvalbumin were determined in liver, spleen, and blood cells by quantitative PCR. Alpha‐2‐macroglobulin in plasma was detected by western blot immunoassay. Clinical signs of infection included skin haemorrhagic lesions and depigmentation. Susceptible and resistant fish were differentiated by lesions >5 cm and absence of lesion at injection site, respectively. Infection significantly increased expression of alpha‐2‐macroglobulin, nattectin and parvalbumin in the liver and spleen but not in blood cell samples. Levels of alpha‐2‐macroglobulin and parvalbumin were significantly increased in liver of susceptible grouper, whereas alpha‐2‐macroglobulin significantly increased in plasma of resistant fish. In conclusion, alpha‐2‐macroglobulin is a potential infection‐resistant biomarker in brown‐marbled grouper and readily detected in fish plasma.
    Keywords Epinephelus fuscoguttatus ; Vibrio parahaemolyticus ; Western blotting ; aquaculture ; biomarkers ; blood ; disease resistance ; dissolved oxygen ; grouper ; immunoassays ; injection site ; liver ; pH ; quantitative polymerase chain reaction ; research ; saline water ; salinity ; spleen
    Language English
    Dates of publication 2022-11
    Size p. 5618-5627.
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 1227359-4
    ISSN 1355-557X
    ISSN 1355-557X
    DOI 10.1111/are.16043
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4230: Show issues

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  4. Article ; Online: Complementary Analytical Platforms of NMR Spectroscopy and LCMS Analysis in the Metabolite Profiling of

    Bustamam, Muhammad Safwan Ahamad / Pantami, Hamza Ahmed / Azizan, Awanis / Shaari, Khozirah / Min, Chong Chou / Abas, Faridah / Nagao, Norio / Maulidiani, Maulidiani / Banerjee, Sanjoy / Sulaiman, Fadzil / Ismail, Intan Safinar

    Marine drugs

    2021  Volume 19, Issue 3

    Abstract: This study was designed to profile the metabolites ... ...

    Abstract This study was designed to profile the metabolites of
    MeSH term(s) Amino Acids/isolation & purification ; Carotenoids/isolation & purification ; Chromatography, Liquid ; Fatty Acids, Unsaturated/isolation & purification ; Haptophyta/metabolism ; Magnetic Resonance Spectroscopy ; Mass Spectrometry ; Metabolomics ; Tandem Mass Spectrometry
    Chemical Substances Amino Acids ; Fatty Acids, Unsaturated ; Carotenoids (36-88-4)
    Language English
    Publishing date 2021-03-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md19030139
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  5. Article: Complementary Analytical Platforms of NMR Spectroscopy and LCMS Analysis in the Metabolite Profiling of Isochrysis galbana

    Bustamam, Muhammad Safwan Ahamad / Pantami, Hamza Ahmed / Azizan, Awanis / Shaari, Khozirah / Min, Chong Chou / Abas, Faridah / Nagao, Norio / Maulidiani, Maulidiani / Banerjee, Sanjoy / Sulaiman, Fadzil / Ismail, Intan Safinar

    Marine drugs. 2021 Mar. 02, v. 19, no. 3

    2021  

    Abstract: This study was designed to profile the metabolites of Isochrysis galbana, an indigenous and less explored microalgae species. ¹H Nuclear Magnetic Resonance (NMR) spectroscopy and Liquid Chromatography-Mass Spectrometry (LCMS) were used to establish the ... ...

    Abstract This study was designed to profile the metabolites of Isochrysis galbana, an indigenous and less explored microalgae species. ¹H Nuclear Magnetic Resonance (NMR) spectroscopy and Liquid Chromatography-Mass Spectrometry (LCMS) were used to establish the metabolite profiles of five different extracts of this microalga, which are hexane (Hex), ethyl acetate (EtOAc), absolute ethanol (EtOH), EtOH:water 1:1 (AqE), and 100% water (Aq). Partial least square discriminant analysis (PLS–DA) of the generated profiles revealed that EtOAc and Aq extracts contain a diverse range of metabolites as compared to the other extracts with a total of twenty-one metabolites, comprising carotenoids, polyunsaturated fatty acids, and amino acids, that were putatively identified from the NMR spectra. Meanwhile, thirty-two metabolites were successfully annotated from the LCMS/MS data, ten of which (palmitic acid, oleic acid, α-linolenic acid, arachidic acid, cholesterol, DHA, DPA, fucoxanthin, astaxanthin, and pheophytin) were similar to those present in the NMR profile. Another eleven glycerophospholipids were discovered using MS/MS-based molecular network (MN) platform. The results of this study, besides providing a better understanding of I.galbana’s chemical make-up, will be of importance in exploring this species potential as a feed ingredient in the aquaculture industry.
    Keywords Isochrysis galbana ; aquaculture industry ; astaxanthin ; cholesterol ; discriminant analysis ; ethanol ; ethyl acetate ; feed composition ; glycerophospholipids ; hexane ; least squares ; liquid chromatography ; metabolites ; microalgae ; nuclear magnetic resonance spectroscopy ; oleic acid ; palmitic acid
    Language English
    Dates of publication 2021-0302
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2175190-0
    ISSN 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md19030139
    Database NAL-Catalogue (AGRICOLA)

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