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  1. Article ; Online: Evaluations and Selections for High Isoflavone Black Soybean Mutants Induced by NaN 3 Treatment

    Toong Long Jeng / Yi Ju Shih / Min Tze Wu / Chiang Sheng Wang / Jih Min Sung

    American Journal of Plant Sciences , Vol 04, Iss 08, Pp 35-

    2013  Volume 40

    Abstract: Sodium azide (NaN 3 ) ( 2 mM ) was used to induce mutation in black soybean variety CRWD for improvement of isofla vone content. The individual selections were made in M6, M7 and M8 generations, respectively. Both high levels of isoflavones and medium ... ...

    Abstract Sodium azide (NaN 3 ) ( 2 mM ) was used to induce mutation in black soybean variety CRWD for improvement of isofla vone content. The individual selections were made in M6, M7 and M8 generations, respectively. Both high levels of isoflavones and medium seed size traits were used as selection criteria. Across three growth seasons, the selected five NaN 3 -induced mutants accumulated more total isoflavones (average increases of 5 % to 25%) than their wild type vari ety CRWD. Four mutants yielded more (average increases of 17 % to 25%) than CRWD. Both mutants SA-M-03 (yel low cotyledon) and SA-M-05 (green cotyledon) are rich in isoflavones and may be useful in food and other applica tions.
    Keywords Black Soybean ; Isoflavones ; Mutation ; Sodium Azide ; Plant culture ; SB1-1110 ; Agriculture ; S ; DOAJ:Plant Sciences ; DOAJ:Agriculture and Food Sciences
    Language English
    Publishing date 2013-08-01T00:00:00Z
    Publisher Scientific Research Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Compositional and Proteomic Analyses of Genetically Modified Broccoli (Brassica oleracea var. italica) Harboring an Agrobacterial Gene

    Mao-Sen Liu / Miau-Hwa Ko / Hui-Chun Li / Shwu-Jene Tsai / Ying-Mi Lai / You-Ming Chang / Min-Tze Wu / Long-Fang O. Chen

    International Journal of Molecular Sciences, Vol 15, Iss 9, Pp 15188-

    2014  Volume 15209

    Abstract: Previously, we showed improved shelf life for agrobacterial isopentenyltransferase (ipt) transgenic broccoli (Brassica oleracea var. italica), with yield comparable to commercial varieties, because of the protection mechanism offered by molecular ... ...

    Abstract Previously, we showed improved shelf life for agrobacterial isopentenyltransferase (ipt) transgenic broccoli (Brassica oleracea var. italica), with yield comparable to commercial varieties, because of the protection mechanism offered by molecular chaperones and stress-related proteins. Here, we used proximate analysis to examine macronutrients, chemical and mineral constituents as well as anti-nutrient and protein changes of ipt-transgenic broccoli and corresponding controls. We also preliminarily assessed safety in mice. Most aspects were comparable between ipt-transgenic broccoli and controls, except for a significant increase in carbohydrate level and a decrease in magnesium content in ipt-transgenic lines 101, 102 and 103, as compared with non-transgenic controls. In addition, the anti-nutrient glucosinolate content was increased and crude fat content decreased in inbred control 104 and transgenic lines as compared with the parental control, “Green King”. Gel-based proteomics detected more than 50 protein spots specifically found in ipt-transgenic broccoli at harvest and after cooking; one-third of these proteins showed homology to potential allergens that also play an important role in plant defense against stresses and senescence. Mice fed levels of ipt-transgenic broccoli mimicking the 120 g/day of broccoli eaten by a 60-kg human adult showed normal growth and immune function. In conclusion, the compositional and proteomic changes attributed to the transgenic ipt gene did not affect the growth and immune response of mice under the feeding regimes examined.
    Keywords allergen ; Brassica oleracea var. italica ; broccoli ; proteomics ; glucosinolate ; isopentenyltransferase (ipt) ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580
    Language English
    Publishing date 2014-08-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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