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  1. Book ; Online: ViWOZ

    Van, Phi Nguyen / Hoang, Tung Cao / Manh, Dung Nguyen / Minh, Quan Nguyen / Quoc, Long Tran

    A Multi-Domain Task-Oriented Dialogue Systems Dataset For Low-resource Language

    2022  

    Abstract: Most of the current task-oriented dialogue systems (ToD), despite having interesting results, are designed for a handful of languages like Chinese and English. Therefore, their performance in low-resource languages is still a significant problem due to ... ...

    Abstract Most of the current task-oriented dialogue systems (ToD), despite having interesting results, are designed for a handful of languages like Chinese and English. Therefore, their performance in low-resource languages is still a significant problem due to the absence of a standard dataset and evaluation policy. To address this problem, we proposed ViWOZ, a fully-annotated Vietnamese task-oriented dialogue dataset. ViWOZ is the first multi-turn, multi-domain tasked oriented dataset in Vietnamese, a low-resource language. The dataset consists of a total of 5,000 dialogues, including 60,946 fully annotated utterances. Furthermore, we provide a comprehensive benchmark of both modular and end-to-end models in low-resource language scenarios. With those characteristics, the ViWOZ dataset enables future studies on creating a multilingual task-oriented dialogue system.
    Keywords Computer Science - Computation and Language ; Computer Science - Artificial Intelligence
    Publishing date 2022-03-15
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Soluble Cytoplasmic Expression and Purification of Immunotoxin HER2(scFv)-PE24B as a Maltose Binding Protein Fusion

    Sangsu Park / Minh Quan Nguyen / Huynh Kim Khanh Ta / Minh Tan Nguyen / Gunsup Lee / Chong Jai Kim / Yeon Jin Jang / Han Choe

    International Journal of Molecular Sciences, Vol 22, Iss 6483, p

    2021  Volume 6483

    Abstract: Human epidermal growth factor receptor 2 (HER-2) is overexpressed in many malignant tumors. The anti-HER2 antibody trastuzumab has been approved for treating HER2-positive early and metastatic breast cancers. Pseudomonas exotoxin A (PE), a bacterial ... ...

    Abstract Human epidermal growth factor receptor 2 (HER-2) is overexpressed in many malignant tumors. The anti-HER2 antibody trastuzumab has been approved for treating HER2-positive early and metastatic breast cancers. Pseudomonas exotoxin A (PE), a bacterial toxin of Pseudomonas aeruginosa , consists of an A-domain with enzymatic activity and a B-domain with cell binding activity. Recombinant immunotoxins comprising the HER2(scFv) single-chain Fv from trastuzumab and the PE24B catalytic fragment of PE display promising cytotoxic effects, but immunotoxins are typically insoluble when expressed in the cytoplasm of Escherichia coli , and thus they require solubilization and refolding. Herein, a recombinant immunotoxin gene was fused with maltose binding protein (MBP) and overexpressed in a soluble form in E. coli . Removal of the MBP yielded stable HER2(scFv)-PE24B at 91% purity; 0.25 mg of pure HER2(scFv)-PE24B was obtained from a 500 mL flask culture. Purified HER2(scFv)-PE24B was tested against four breast cancer cell lines differing in their surface HER2 level. The immunotoxin showed stronger cytotoxicity than HER2(scFv) or PE24B alone. The IC 50 values for HER2(scFv)-PE24B were 28.1 ± 2.5 pM ( n = 9) and 19 ± 1.4 pM ( n = 9) for high HER2-positive cell lines SKBR3 and BT-474, respectively, but its cytotoxicity was lower against MDA-MB-231 and MCF7. Thus, fusion with MBP can facilitate the soluble expression and purification of scFv immunotoxins.
    Keywords protein expression ; protein purification ; immunotoxin ; HER2(scFv)-PE24B ; maltose binding protein ; trastuzumab ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 572
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Soluble Prokaryotic Overexpression and Purification of Human GM-CSF Using the Protein Disulfide Isomerase b′a′ Domain

    Thi Kieu Oanh Nguyen / Thi Luong Vu / Minh Quan Nguyen / Huynh Kim Khanh Ta / Kyoung Sun Park / Soo Hyeon Kim / Chong Jai Kim / Yeon Jin Jang / Han Choe

    International Journal of Molecular Sciences, Vol 22, Iss 5267, p

    2021  Volume 5267

    Abstract: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a member of the colony-stimulating factor (CSF) family, which functions to enhance the proliferation and differentiation of hematopoietic stem cells and other hematopoietic lineages such as ... ...

    Abstract Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a member of the colony-stimulating factor (CSF) family, which functions to enhance the proliferation and differentiation of hematopoietic stem cells and other hematopoietic lineages such as neutrophils, dendritic cells, or macrophages. These proteins have thus generated considerable interest in clinical therapy research. A current obstacle to the prokaryotic production of human GM-CSF (hGM-CSF) is its low solubility when overexpressed and subsequent complex refolding processes. In our present study, the solubility of hGM-CSF was examined when combined with three N-terminal fusion tags in five E. coli strains at three different expression temperatures. In the five E. coli strains BL21 (DE3), ClearColi BL21 (DE3), LOBSTR, SHuffle T7 and Origami2 (DE3), the hexahistidine-tagged hGM-CSF showed the best expression but was insoluble in all cases at each examined temperature. Tagging with the maltose-binding protein (MBP) and the b′a′ domain of protein disulfide isomerase (PDIb′a′) greatly improved the soluble overexpression of hGM-CSF at 30 °C and 18 °C. The solubility was not improved using the Origami2 (DE3) and SHuffle T7 strains that have been engineered for disulfide bond formation. Two conventional chromatographic steps were used to purify hGM-CSF from the overexpressed PDIb′a′-hGM-CSF produced in ClearColi BL21 (DE3). In the experiment, 0.65 mg of hGM-CSF was isolated from a 0.5 L flask culture of these E. coli and showed a 98% purity by SDS-PAGE analysis and silver staining. The bioactivity of this purified hGM-CSF was measured at an EC 50 of 16.4 ± 2 pM by a CCK8 assay in TF-1 human erythroleukemia cells.
    Keywords hGM-CSF ; MBP ; PDI ; ClearColi ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 616
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Associated Factors of Hypertension in Women and Men in Vietnam: A Cross-Sectional Study.

    Quoc Cuong, Tran / Van Bao, Le / Anh Tuan, Nguyen / Van Thang, Vo / Minh Quan, Nguyen / Yang, Shwu-Huey / Duong, Tuyen Van

    International journal of environmental research and public health

    2019  Volume 16, Issue 23

    Abstract: Background: Hypertension is a direct cardiovascular disease risk. It causes a heavy burden on the healthcare system globally. We aim to assess hypertension occurrence and its associated factors among women and men in Vietnam.: Methods: A cross- ... ...

    Abstract Background: Hypertension is a direct cardiovascular disease risk. It causes a heavy burden on the healthcare system globally. We aim to assess hypertension occurrence and its associated factors among women and men in Vietnam.
    Methods: A cross-sectional study was conducted from January to February 2019 on 2203 community-dwelling women and men aged 18 years or above. Participants' characteristics, comorbidity, behaviors, and physical measures were evaluated. Hypertension was classified as systolic/diastolic blood pressure ≥140/90 mmHg or using antihypertensive medication. We analyzed data using logistic regression models.
    Results: The prevalence of hypertension was 24.3% (20.9% in women, 29.1% in men). For women, older age (odds ratio, OR, 6.80-12.41;
    MeSH term(s) Adolescent ; Adult ; Age Factors ; Aged ; Antihypertensive Agents/therapeutic use ; Cross-Sectional Studies ; Diabetes Mellitus/epidemiology ; Female ; Humans ; Hypertension/drug therapy ; Hypertension/epidemiology ; Logistic Models ; Male ; Middle Aged ; Odds Ratio ; Overweight/epidemiology ; Prevalence ; Risk Factors ; Sex Factors ; Smoking/epidemiology ; Socioeconomic Factors ; Sodium Chloride, Dietary ; Vietnam/epidemiology ; Young Adult
    Chemical Substances Antihypertensive Agents ; Sodium Chloride, Dietary
    Language English
    Publishing date 2019-11-26
    Publishing country Switzerland
    Document type Journal Article
    ISSN 1660-4601
    ISSN (online) 1660-4601
    DOI 10.3390/ijerph16234714
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Evaluation of the leaching behavior of incineration bottom ash using seawater: A comparison with standard leaching tests

    Lin, Wenlin Yvonne / Alec Liu / Fei Ren / Jin Rui Ivan Ho / Jing-Yuan Wang / Kim Soon Heng / Minh Quan Nguyen / Omar Ahmad Bin Mohamed Noh / Xue Dong Zhou

    Waste management. 2017 Apr., v. 62

    2017  

    Abstract: Batch and column tests were conducted on untreated incineration bottom ash (IBA) samples from two incineration plants in Singapore, using seawater as the leachant. The main objective of this study was to investigate the change in the leaching behavior of ...

    Abstract Batch and column tests were conducted on untreated incineration bottom ash (IBA) samples from two incineration plants in Singapore, using seawater as the leachant. The main objective of this study was to investigate the change in the leaching behavior of certain elements (i.e. As, Cd, Cr, Cu, Ni, Pb, Sb, Se and Zn) when IBA comes into contact with seawater. Such an investigation using seawater as leachant was not commonly carried out when investigating leaching behavior in IBA. The leaching tests were then carried out on the same IBA samples using DI water, as a comparison. Lower level of leaching was observed for Pb and Zn when seawater was used as the leachant. Cr and Sb showed significant cumulative release at Liquid-to-Solids (L/S) ratio 5 in the seawater column leaching. The influence of Dissolved Organic Carbon (DOC) on Cu leaching seems to decrease after L/S 2 when using seawater in the column test. Although the leaching behavior of IBA was affected when seawater was used, for the column test, there was no significant difference during the initial release when compared to DI water. The initial L/S fractions collected were important as the low L/S ratios represent the pore water concentration and the maximum output in an actual application. The results from this study would be useful for the future study on using IBA in marine applications.
    Keywords antimony ; arsenic ; bottom ash ; cadmium ; chromium ; copper ; dissolved organic carbon ; leaching ; lead ; nickel ; seawater ; selenium ; waste management ; zinc ; Singapore
    Language English
    Dates of publication 2017-04
    Size p. 139-146.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2001471-5
    ISSN 1879-2456 ; 0956-053X
    ISSN (online) 1879-2456
    ISSN 0956-053X
    DOI 10.1016/j.wasman.2016.12.003
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: A multi centre randomized open label trial of chloroquine for the treatment of adults with SARS-CoV-2 infection in Vietnam [version 1;peer review: awaiting peer review]

    Kestelyn, Evelyne Nguyen Thi Phuong Dung Yen Lam Minh Le Manh Hung Nguyen Minh Quan Nguyen Thanh Dung Ngo Ngoc Quang Minh Tran Chanh Xuan Nguyen Thanh Phong Van Ninh Thi Thanh Donovan Joseph Tran Nguyen Hoang Tu Le Thanh Hoang Nhat Nguyen Thanh Truong Dinh Nguyen Huy Man Huynh Phuong Thao Nghiêm My Ngoc Vo Thanh Lam Huynh Hong Phat Phan Minh Phuong Geskus Ronald B. / Vo Thi Nhi, Ha Nguyen Ngo Quang Hien Tran Tinh Le Van Tan Thwaites Guy Day Jeremy Nguyen Van Vinh Chau Group Oucru Covid-Research

    Abstract: Background: COVID-19 is a respiratory disease caused by a novel coronavirus (SARS-CoV-2) and causes substantial morbidity and mortality There is currently no vaccine to prevent COVID-19 or therapeutic agent to treat COVID-19 This clinical trial is ... ...

    Abstract Background: COVID-19 is a respiratory disease caused by a novel coronavirus (SARS-CoV-2) and causes substantial morbidity and mortality There is currently no vaccine to prevent COVID-19 or therapeutic agent to treat COVID-19 This clinical trial is designed to evaluate chloroquine as a potential therapeutic for the treatment of hospitalised people with COVID-19 We hypothesise that chloroquine slows viral replication in patients with COVID-19, attenuating the infection, and resulting in more rapid decline of viral load in throat/nose swabs This viral attenuation should be associated with improved patient outcomes Method: The study will start with a 10-patient prospective observational pilot study following the same entry and exclusion criteria as for the randomized trial and undergoing the same procedures The main study is an open label, randomised, controlled trial with two parallel arms of standard of care (control arm) versus standard of care with 10 days of chloroquine (intervention arm) with a loading dose over the first 24 hours, followed by 300mg base orally once daily for nine days The study will recruit patients in three sites in Ho ChMinh City, Vietnam: the Hospital for Tropical Diseases, the Cu ChField Hospital, and the Can Gio COVID hospital The primary endpoint is the time to viral clearance from throat/nose swab, defined as the time following randomization until the midpoint between the last positive and the first of the negative throat/nose swabs Viral presence will be determined using RT-PCR to detect SARS-CoV-2 RNA Discussion: The results of the study will add to the evidence-based guidelines for management of COVID-19 Given the enormous experience of its use in malaria chemoprophylaxis, excellent safety and tolerability profile, and its very low cost, if proved effective then chloroquine would be a readily deployable and affordable treatment for patients with COVID-19 Trial registration: Clinicaltrials gov NCT04328493 31/03/2020
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #2103
    Database COVID19

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