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Article ; Online: Applicability and costs of nanofiltration in combination with photocatalysis for the treatment of dye house effluents

Wolfgang M. Samhaber / Minh Tan Nguyen

Beilstein Journal of Nanotechnology, Vol 5, Iss 1, Pp 476-

2014 Volume 484

Abstract: Nanofiltration (NF) is a capable method for the separation of dyes, which can support and even improve the applicability of photocatalysis in effluent-treatment processes. The membrane process usually will need a special pre-treatment to avoid ... ...

Abstract	Nanofiltration (NF) is a capable method for the separation of dyes, which can support and even improve the applicability of photocatalysis in effluent-treatment processes. The membrane process usually will need a special pre-treatment to avoid precipitation and fouling on the membrane surface. Conceptually NF can be applied in the pre-treatment prior to the catalytic reactor or in connection with the reactor to separate the liquid phase from the reaction system and to recycle finely suspended catalysts and/or organic compounds. When concerning such reaction systems on a bigger scale, cost figures will prove the usefulness of those concepts. Different applications of photocatalysis on the lab-scale have been published in recent years. Membrane technology is used almost in all those processes and an overview will be given of those recently published systems that have been reported to be potentially useful for a further scale-up. NF membranes are mostly used for the more sophisticated separation step of these processes and the additional costs of the NF treatment, without any associated equipments, will be described and illustrated. The total specific costs of industrial NF treatment processes in usefully adjusted and designed plants range from 1 to 6 US$/m3 treated effluent. Combination concepts will have a good precondition for further development and upscaling, if the NF costs discussed here in detail will be, together with the costs of photocatalysis, economically acceptable.
Keywords	dye industry effluent ; environmental ; membrane ; nanofiltration ; photocatalysis ; UV ; Technology ; T ; Chemical technology ; TP1-1185 ; Science ; Q ; Physics ; QC1-999
Subject code	660
Language	English
Publishing date	2014-04-01T00:00:00Z
Publisher	Beilstein-Institut
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Soluble Cytoplasmic Expression and Purification of Immunotoxin HER2(scFv)-PE24B as a Maltose Binding Protein Fusion

Sangsu Park / Minh Quan Nguyen / Huynh Kim Khanh Ta / Minh Tan Nguyen / Gunsup Lee / Chong Jai Kim / Yeon Jin Jang / Han Choe

International Journal of Molecular Sciences, Vol 22, Iss 6483, p

2021 Volume 6483

Abstract: Human epidermal growth factor receptor 2 (HER-2) is overexpressed in many malignant tumors. The anti-HER2 antibody trastuzumab has been approved for treating HER2-positive early and metastatic breast cancers. Pseudomonas exotoxin A (PE), a bacterial ... ...

Abstract	Human epidermal growth factor receptor 2 (HER-2) is overexpressed in many malignant tumors. The anti-HER2 antibody trastuzumab has been approved for treating HER2-positive early and metastatic breast cancers. Pseudomonas exotoxin A (PE), a bacterial toxin of Pseudomonas aeruginosa , consists of an A-domain with enzymatic activity and a B-domain with cell binding activity. Recombinant immunotoxins comprising the HER2(scFv) single-chain Fv from trastuzumab and the PE24B catalytic fragment of PE display promising cytotoxic effects, but immunotoxins are typically insoluble when expressed in the cytoplasm of Escherichia coli , and thus they require solubilization and refolding. Herein, a recombinant immunotoxin gene was fused with maltose binding protein (MBP) and overexpressed in a soluble form in E. coli . Removal of the MBP yielded stable HER2(scFv)-PE24B at 91% purity; 0.25 mg of pure HER2(scFv)-PE24B was obtained from a 500 mL flask culture. Purified HER2(scFv)-PE24B was tested against four breast cancer cell lines differing in their surface HER2 level. The immunotoxin showed stronger cytotoxicity than HER2(scFv) or PE24B alone. The IC 50 values for HER2(scFv)-PE24B were 28.1 ± 2.5 pM ( n = 9) and 19 ± 1.4 pM ( n = 9) for high HER2-positive cell lines SKBR3 and BT-474, respectively, but its cytotoxicity was lower against MDA-MB-231 and MCF7. Thus, fusion with MBP can facilitate the soluble expression and purification of scFv immunotoxins.
Keywords	protein expression ; protein purification ; immunotoxin ; HER2(scFv)-PE24B ; maltose binding protein ; trastuzumab ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	572
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Antioxidant and Antimicrobial Activities of the Extracts from Different Garcinia Species

Nguyen Hoai Nguyen / Minh Tan Nguyen / Hiep Dinh Nguyen / Phuoc Dien Pham / Ut Dong Thach / Binh T. D. Trinh / Ly T. T. Nguyen / Son V. Dang / Anh Thu Do / Bich Hang Do

Evidence-Based Complementary and Alternative Medicine, Vol

2021 Volume 2021

Abstract: Background. Garcinia is a large genus which has promising bioactivities. However, the properties of many Garcinia species have not been investigated thoroughly. Aim. To determine the antioxidant and antimicrobial capabilities of the extracts from ... ...

Abstract	Background. Garcinia is a large genus which has promising bioactivities. However, the properties of many Garcinia species have not been investigated thoroughly. Aim. To determine the antioxidant and antimicrobial capabilities of the extracts from different Garcinia species. Methodology. Six Garcinia species, including Garcinia fusca, Garcinia hopii, Garcinia planchonii, Garcinia nigrolineata, Garcinia gaudichaudii, and Garcinia tinctoria were extracted using n-hexane, ethyl acetate, and methanol, producing n-hexane extract (HE), ethyl acetate extract (EAE), and methanol extract (ME). After that, the total polyphenol content was evaluated using Folin–Ciocalteu assay. DPPH, hydroxyl radical scavenging, and total antioxidant capacity assays were performed to test the antioxidant activity. Subsequently, the antimicrobial activities against Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacterial strains were assessed using Kirby Bauer and the broth microdilution methods. Results. Many Garcinia extracts contained high total polyphenol content consisting of ME of G. hopii ad G. tinctoria, and EAE of G. planchonii and G. tinctoria. The EAE of G. tinctoria showed effective antioxidant capacity (IC50 = 1.5 µg/mL). Additionally, the EAE of G. gaudichaudii was effective against Gram-positive bacteria with minimal inhibition concentration (MIC) of 15.625–25 µg/mL whereas ME of G. planchonii was effective against both Gram-positive bacteria (MIC = 160 µg/mL) and Gram-negative bacteria (MIC = 75 µg/mL). Conclusion. Several extracts of Garcinia species demonstrated valuable antioxidant and antimicrobial properties.
Keywords	Other systems of medicine ; RZ201-999
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Novel Bacterial Production of Two Different Bioactive Forms of Human Stem-Cell Factor

Eunyoung Lee / Michelle Novais de Paula / Sangki Baek / Huynh Kim Khanh Ta / Minh Tan Nguyen / Taeck-Hyun Jeong / Chong Jai Kim / Yeon Jin Jang / Han Choe

International Journal of Molecular Sciences, Vol 22, Iss 6361, p

2021 Volume 6361

Abstract: Human stem-cell factor (hSCF) stimulates the survival, proliferation, and differentiation of hematopoietic cells by binding to the c-Kit receptor. Various applications of hSCF require the efficient and reliable production of hSCF. hSCF exists in three ... ...

Abstract	Human stem-cell factor (hSCF) stimulates the survival, proliferation, and differentiation of hematopoietic cells by binding to the c-Kit receptor. Various applications of hSCF require the efficient and reliable production of hSCF. hSCF exists in three forms: as two membrane-spanning proteins hSCF248 and hSCF229 and truncated soluble N -terminal protein hSCF164. hSCF164 is known to be insoluble when expressed in Escherichia coli cytoplasm, requiring a complex refolding procedure. The activity of hSCF248 has never been studied. Here, we investigated novel production methods for recombinant hSCF164 and hSCF248 without the refolding process. To increase the solubility of hSCF164, maltose-binding protein (MBP) and protein disulfide isomerase b’a’ domain (PDIb’a’) tags were attached to the N -terminus of hSCF164. These fusion proteins were overexpressed in soluble form in the Origami 2(DE3) E. coli strain. These solubilization effects were enhanced at a low temperature. His-hSCF248, the poly-His tagged form of hSCF248, was expressed in a highly soluble form without a solubilization tag protein, which was unexpected because His-hSCF248 contains a transmembrane domain. hSCF164 was purified using affinity and ion-exchange chromatography, and His-hSCF248 was purified by ion-exchange and gel filtration chromatography. The purified proteins stimulated the proliferation of TF-1 cells. Interestingly, the EC 50 value of His-hSCF248 was 1 pg/mL, 100-fold lower than 9 ng/mL hSCF164. Additionally, His-hSCF248 decreased the doubling time, increased the proportion of S and G2/M stages in the cell cycle, and increased the c-Myc expression at a 1000-fold lower concentration than hSCF164. In conclusion, His-hSCF248 was expressed in a soluble form in E. coli and had stronger activity than hSCF164. The molecular chaperone, MBP, enabled the soluble overexpression of hSCF164.
Keywords	human stem-cell factor ; hSCF248 ; hSCF164 ; MBP ; recombinant protein ; soluble protein ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	500
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Ribonuclease J-Mediated mRNA Turnover Modulates Cell Shape, Metabolism and Virulence in Corynebacterium diphtheriae

Truc Thanh Luong / Minh Tan Nguyen / Yi-Wei Chen / Chungyu Chang / Ju Huck Lee / Manuel Wittchen / HyLam Ton-That / Melissa Cruz / Danielle A. Garsin / Asis Das / Andreas Tauch / Hung Ton-That

Microorganisms, Vol 9, Iss 2, p

2021 Volume 389

Abstract: Controlled RNA degradation is a crucial process in bacterial cell biology for maintaining proper transcriptome homeostasis and adaptation to changing environments. mRNA turnover in many Gram-positive bacteria involves a specialized ribonuclease called ... ...

Abstract	Controlled RNA degradation is a crucial process in bacterial cell biology for maintaining proper transcriptome homeostasis and adaptation to changing environments. mRNA turnover in many Gram-positive bacteria involves a specialized ribonuclease called RNase J (RnJ). To date, however, nothing is known about this process in the diphtheria-causative pathogen Corynebacterium diphtheriae , nor is known the identity of this ribonuclease in this organism. Here, we report that C. diphtheriae DIP1463 encodes a predicted RnJ homolog, comprised of a conserved N-terminal β-lactamase domain, followed by β-CASP and C-terminal domains. A recombinant protein encompassing the β-lactamase domain alone displays 5′-exoribonuclease activity, which is abolished by alanine-substitution of the conserved catalytic residues His 186 and His 188 . Intriguingly, deletion of DIP1463/ rnj in C. diphtheriae reduces bacterial growth and generates cell shape abnormality with markedly augmented cell width. Comparative RNA-seq analysis revealed that RnJ controls a large regulon encoding many factors predicted to be involved in biosynthesis, regulation, transport, and iron acquisition. One upregulated gene in the ∆ rnj mutant is ftsH , coding for a membrane protease (FtsH) involved in cell division, whose overexpression in the wild-type strain also caused cell-width augmentation. Critically, the ∆ rnj mutant is severely attenuated in virulence in a Caenorhabditis elegans model of infection, while the FtsH-overexpressing and toxin-less strains exhibit full virulence as the wild-type strain. Evidently, RNase J is a key ribonuclease in C. diphtheriae that post-transcriptionally influences the expression of numerous factors vital to corynebacterial cell physiology and virulence. Our findings have significant implications for basic biological processes and mechanisms of corynebacterial pathogenesis.
Keywords	Corynebacterium diphtheriae ; actinobacterium ; ribonuclease ; RNase J ; virulence ; Caenorhabditis elegans ; Biology (General) ; QH301-705.5
Subject code	571 ; 572
Language	English
Publishing date	2021-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Impact of ultrasonic dispersion on the photocatalytic activity of titania aggregates

Hoai Nga Le / Frank Babick / Klaus Kühn / Minh Tan Nguyen / Michael Stintz / Gianaurelio Cuniberti

Beilstein Journal of Nanotechnology, Vol 6, Iss 1, Pp 2423-

2015 Volume 2430

Abstract: The effectiveness of photocatalytic materials increases with the specific surface area, thus nanoscale photocatalyst particles are preferred. However, such nanomaterials are frequently found in an aggregated state, which may reduce the photocatalytic ... ...

Abstract	The effectiveness of photocatalytic materials increases with the specific surface area, thus nanoscale photocatalyst particles are preferred. However, such nanomaterials are frequently found in an aggregated state, which may reduce the photocatalytic activity due to internal obscuration and the extended diffusion path of the molecules to be treated. This paper investigates the effect of aggregate size on the photocatalytic activity of pyrogenic titania (Aeroxide® P25, Evonik), which is widely used in fundamental photocatalysis research. Well-defined and reproducible aggregate sizes were achieved by ultrasonic dispersion. The photocatalytic activity was examined by the color removal of methylene blue (MB) with a laboratory-scale setup based on a plug flow reactor (PFR) and planar UV illumination. The process parameters such as flow regime, optical path length and UV intensity are well-defined and can be varied. Our results firstly show that a complete dispersion of the P25 aggregates is not practical. Secondly, the photocatalytic activity is not further increased beyond a certain degree of dispersion, which probably corresponds to a critical size for which UV irradiation can penetrate the aggregate without significant obscuration.
Keywords	AOPs ; reaction rate constant ; turbidity ; ultrasonic energy ; wastewater treatment ; Technology ; T ; Chemical technology ; TP1-1185 ; Science ; Q ; Physics ; QC1-999
Subject code	535
Language	English
Publishing date	2015-12-01T00:00:00Z
Publisher	Beilstein-Institut
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Prokaryotic soluble overexpression and purification of oncostatin M using a fusion approach and genetically engineered E. coli strains

Minh Tan Nguyen / Musharrat Jahan Prima / Jung-A. Song / Julee Kim / Bich Hang Do / Jiwon Yoo / Sangsu Park / Jaepyeong Jang / Sunju Lee / Eunyoung Lee / Michelle de Paula Novais / Hyeon-Beom Seo / Seon-yeong Lee / Mi-La Cho / Chong Jai Kim / Yeon Jin Jang / Han Choe

Scientific Reports, Vol 9, Iss 1, Pp 1-

2019 Volume 13

Abstract: Abstract Human Oncostatin M (OSM), initially discovered as a tumour inhibitory factor secreted from U-937 cells, is a gp130 (IL-6/LIF) cytokine family member that exhibits pleiotropic effects in inflammation, haematopoiesis, skeletal tissue alteration, ... ...

Abstract	Abstract Human Oncostatin M (OSM), initially discovered as a tumour inhibitory factor secreted from U-937 cells, is a gp130 (IL-6/LIF) cytokine family member that exhibits pleiotropic effects in inflammation, haematopoiesis, skeletal tissue alteration, liver regeneration, cardiovascular and metabolic diseases. Cytoplasmic expression of OSM in Escherichia coli results in inclusion bodies, and complex solubilisation, refolding and purification is required to prepare bioactive protein. Herein, eight N-terminal fusion variants of OSM with hexahistidine (His6) tag and seven solubility-enhancing tags, including thioredoxin (Trx), small ubiquitin-related modifier (Sumo), glutathione S-transferase (GST), maltose-binding protein (MBP), N-utilisation substance protein A (Nusa), human protein disulphide isomerase (PDI) and the b‘a’ domain of PDI (PDIb‘a’), were tested for soluble OSM expression in E. coli. The His6-OSM plasmid was also introduced into genetically engineered Origami 2 and SHuffle strains to test expression of the protein. At 18 °C, MBP-tagged OSM was highly expressed and solubility was dramatically enhanced. In addition, His6-OSM was more highly expressed and soluble in Origami 2 and SHuffle strains than in BL21(DE3). MBP-OSM and His6-OSM were purified more than 95% with yields of 11.02 mg and 3.27 mg from a 500 mL culture. Protein identity was confirmed by mass spectroscopy, and bioactivity was demonstrated by in vitro inhibition of Th17 cell differentiation.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2019-09-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Correction

Minh Tan Nguyen / Martin Krupa / Bon-Kyung Koo / Jung-A Song / Thu Trang Thi Vu / Bich Hang Do / Anh Ngoc Nguyen / Taewook Seo / Jiwon Yoo / Boram Jeong / Jonghwa Jin / Kyung Jin Lee / Heung-Bum Oh / Han Choe

PLoS ONE, Vol 12, Iss 1, p e

Prokaryotic soluble overexpression and purification of human VEGF165 by fusion to a maltose binding protein tag.

2017 Volume 0170602

Abstract: This corrects the article DOI:10.1371/journal.pone.0156296.]. ...

Abstract	[This corrects the article DOI:10.1371/journal.pone.0156296.].
Keywords	Medicine ; R ; Science ; Q
Language	English
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Correction

PLoS ONE, Vol 12, Iss 1, p e

Prokaryotic soluble overexpression and purification of human VEGF165 by fusion to a maltose binding protein tag.

2017 Volume 0170602

Abstract: This corrects the article DOI:10.1371/journal.pone.0156296.]. ...

Abstract	[This corrects the article DOI:10.1371/journal.pone.0156296.].
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Granulocyte colony-stimulating factor (GCSF) fused with Fc Domain produced from E. coli is less effective than Polyethylene Glycol-conjugated GCSF

Bich Hang Do / Hyo Jeong Kang / Jung-A Song / Minh Tan Nguyen / Sangsu Park / Jiwon Yoo / Anh Ngoc Nguyen / Grace G. Kwon / Jaepyeong Jang / Mihee Jang / Sunju Lee / Seoungjun So / Seongrak Sim / Kyung Jin Lee / Mark J. Osborn / Han Choe

Scientific Reports, Vol 7, Iss 1, Pp 1-

2017 Volume 9

Abstract: Abstract Human granulocyte colony-stimulating factor (GCSF) is a well-known cytokine for neutropenia treatment. However, daily injections are required due to the short circulating half-life of the protein. To overcome this bottleneck, we fused GCSF with ... ...

Abstract	Abstract Human granulocyte colony-stimulating factor (GCSF) is a well-known cytokine for neutropenia treatment. However, daily injections are required due to the short circulating half-life of the protein. To overcome this bottleneck, we fused GCSF with the Fc domain of IgG1 at the C terminus (GCSF-Fc) and with the maltose binding protein (MBP) tag at the N-terminus and expressed it as a soluble protein in the cytoplasm of E. coli. We also conjugated PEG aldehyde to GCSF to make PEG-GCSF. The bioactivities of GCSF-Fc and PEG-GCSF were similar to native GCSF using the mouse M-NFS-60 myelogenous leukemia cell line. The EC50 dose-response curves for GCSF, GCSF-Fc and PEG-GCSF were 37 ± 12 pM, 75 ± 13.5 pM and 46 ± 5.5 pM, respectively. When the proteins were injected into neutropenic rats, the group injected with PEG-GCSF showed the highest and fastest recovery of neutrophils, followed by GCSF-Fc and GCSF. ELISA assay revealed the PEG-GCSF had the longest plasma circulation (>72 h), followed by GCSF-Fc (>48 h) and GCSF (~24 h), which is consistent with the in vivo activities of the proteins. In summary, the GCSF-Fc purified from E. coli was not as efficient as PEG-GCSF in treating neutropenic rats.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2017-07-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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