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Article ; Online: Delirium Diagnosis, Complication Recognition, and Treatment Knowledge among Nurses in an Italian Local Hospital: A Cross-Sectional Study.

Ceccarelli, Andrea / Ballarin, Maddalena / Montalti, Marco / Ceccarelli, Paola / Mazzini, Silvia / Minotti, Alice / Gori, Davide / Senni, Marco

Nursing reports (Pavia, Italy)

2024 Volume 14, Issue 2, Page(s) 767–776

Abstract: Delirium, a multifactorial condition with an acute onset and diverse clinical manifestations, poses a significant challenge in the care of hospitalized individuals aged 65 years and older. This study aimed to evaluate the level of knowledge among nursing ...

Abstract	Delirium, a multifactorial condition with an acute onset and diverse clinical manifestations, poses a significant challenge in the care of hospitalized individuals aged 65 years and older. This study aimed to evaluate the level of knowledge among nursing healthcare personnel regarding the diagnosis, recognition of complications, and treatment of delirium. A paper questionnaire consisting of 18 multiple-choice questions was distributed to nurses in twelve operational units located in four facilities within a local hospital in a specific geographical region under the jurisdiction of the Romagna Local Health Authority in Italy. Out of 194 respondents, the overall acceptance rate was 64.2%. The findings revealed an insufficient understanding of delirium among the nursing staff, with more than 40% of respondents answering incorrectly to five out of nine questions related to delirium knowledge, diagnosis, prevention, and treatment. Notably, gender emerged as a significant determinant, with female participants exhibiting a substantial odds ratio (OR) of 3.50 (
Language	English
Publishing date	2024-03-27
Publishing country	Switzerland
Document type	Journal Article
ISSN	2039-4403
ISSN (online)	2039-4403
DOI	10.3390/nursrep14020059
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Article: Identifying novel age-modulating compounds and quantifying cellular aging using novel computational framework for evaluating transcriptional age.

Zhang, Chao / Saurat, Nathalie / Cornacchia, Daniela / Chung, Sun Young / Sikder, Trisha / Minotti, Andrew / Studer, Lorenz / Betel, Doron

bioRxiv : the preprint server for biology

2023

Abstract: The differentiation of human pluripotent stem cells (hPSCs) provides access to most cell types and tissues. However, hPSC-derived lineages capture a fetal-stage of development and methods to accelerate progression to an aged identity are limited. ... ...

Abstract	The differentiation of human pluripotent stem cells (hPSCs) provides access to most cell types and tissues. However, hPSC-derived lineages capture a fetal-stage of development and methods to accelerate progression to an aged identity are limited. Understanding the factors driving cellular age and rejuvenation is also essential for efforts aimed at extending human life and health span. A prerequisite for such studies is the development of methods to score cellular age and simple readouts to assess the relative impact of various age modifying strategies. Here we established a transcriptional score (RNAge) in young versus old primary fibroblasts, frontal cortex and substantia nigra tissue. We validated the score in independent RNA-seq datasets and demonstrated a strong cell and tissue specificity. In fibroblasts we observed a reset of RNAge during iPSC reprogramming while direct reprogramming of aged fibroblasts to induced neurons (iN) resulted in the maintenance of both a neuronal and a fibroblast aging signature. Increased RNAge in hPSC-derived neurons was confirmed for several age-inducing strategies such as SATB1 loss, progerin expression or chemical induction of senescence (SLO). Using RNAge as a probe set, we next performed an in-silico screen using the LINCS L1000 dataset. We identified and validated several novel age-inducing and rejuvenating compounds, and we observed that RNAage captures age-related changes associated with distinct cellular hallmarks of age. Our study presents a simple tool to score age manipulations and identifies compounds that greatly expand the toolset of age-modifying strategies in hPSC derived lineages.
Language	English
Publishing date	2023-10-04
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.07.03.547539
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Article: Green H

Esposito, Elisa / Minotti, Angelo / Fontananova, Enrica / Longo, Mariagiulia / Jansen, Johannnes Carolus / Figoli, Alberto

Membranes

2021 Volume 12, Issue 1

Abstract: Low-temperature electrolysis by using polymer electrolyte membranes (PEM) can play an important role in hydrogen energy transition. This work presents a study on the performance of a proton exchange membrane in the water electrolysis process at room ... ...

Abstract	Low-temperature electrolysis by using polymer electrolyte membranes (PEM) can play an important role in hydrogen energy transition. This work presents a study on the performance of a proton exchange membrane in the water electrolysis process at room temperature and atmospheric pressure. In the perspective of applications that need a device with small volume and low weight, a miniaturized electrolysis cell with a 36 cm
Language	English
Publishing date	2021-12-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2614641-1
ISSN	2077-0375
ISSN	2077-0375
DOI	10.3390/membranes12010015
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Article: Healthcare Service Quality Evaluation in a Community-Oriented Primary Care Center, Italy.

Ceccarelli, Andrea / Minotti, Alice / Senni, Marco / Pellegrini, Luca / Benati, Giuseppe / Ceccarelli, Paola / Federici, Andrea / Mazzini, Silvia / Reali, Chiara / Sintoni, Francesco / Gori, Davide / Montalti, Marco

Healthcare (Basel, Switzerland)

2023 Volume 11, Issue 17

Abstract: Community-oriented primary care (COPC) is an inclusive healthcare approach that combines individual care with a population-based outlook, striving to offer effective and equitable services. This study concentrates on assessing the perceived quality of a " ...

Abstract	Community-oriented primary care (COPC) is an inclusive healthcare approach that combines individual care with a population-based outlook, striving to offer effective and equitable services. This study concentrates on assessing the perceived quality of a "Casa della Comunità" (CdC) implemented by the Romagna Local Health Authority, which embraces the COPC model. Through the examination of user experiences, the study aims to comprehend the influence of the CdC's care delivery model on the community's perception of service quality. From 13-18 March 2023, paper questionnaires were distributed by trained healthcare professionals and volunteers. The cross-sectional study enrolled participants aged 18 or older, capable of understanding written Italian, and willing to take part voluntarily. A total of 741 questionnaires were collected, resulting in an overall acceptance rate of 85.6%. Among the respondents, 37.9% were female, with an average age of 55.4 ± 16.2 years. While the respondents generally held a positive view of the quality, the results displayed varying levels of satisfaction across the different areas. Multivariate analysis revealed significant associations between factors such as gender, employment status, financial resources, education level, and distance from the healthcare center with the perceived quality of the facility in terms of accessibility, environment, staff, continuity of care, and overall satisfaction. The study yielded valuable insights, identifying strengths and areas for improvement and underscoring the importance of ongoing monitoring studies to enhance patient satisfaction continuously.
Language	English
Publishing date	2023-08-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2721009-1
ISSN	2227-9032
ISSN	2227-9032
DOI	10.3390/healthcare11172396
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Article ; Online: Th22 response induced by Mycobacterium tuberculosis strains is closely related to severity of pulmonary lesions and bacillary load in patients with multi-drug-resistant tuberculosis.

Imperiale, B R / García, A / Minotti, A / González Montaner, P / Moracho, L / Morcillo, N S / Palmero, D J / Sasiain, M Del Carmen / de la Barrera, S

Clinical and experimental immunology

2020 Volume 203, Issue 2, Page(s) 267–280

Abstract: The role of interleukin-22 (IL-22) in the pathogenesis or tissue repair in human tuberculosis (TB) remains to be established. Here, we aimed to explore the ex-vivo and in-vitro T helper 22 (Th22) response in TB patients and healthy donors (HD) induced by ...

Abstract	The role of interleukin-22 (IL-22) in the pathogenesis or tissue repair in human tuberculosis (TB) remains to be established. Here, we aimed to explore the ex-vivo and in-vitro T helper 22 (Th22) response in TB patients and healthy donors (HD) induced by different local multi-drug-resistant (MDR) Mvcobacterium tuberculosis (Mtb) strains. For this purpose, peripheral blood mononuclear cells from drug-susceptible (S-TB) MDR-TB patients and HD were stimulated with local MDR strains and the laboratory strain H37Rv. IL-22 and IL-17 expression and senescent status were assessed in CD4
MeSH term(s)	Adult ; Antigens, Bacterial/immunology ; CD4-Positive T-Lymphocytes/immunology ; CD57 Antigens/immunology ; CD8-Positive T-Lymphocytes/immunology ; Female ; Humans ; Interferon-gamma/immunology ; Interleukin-17/immunology ; Interleukins/immunology ; Leukocytes, Mononuclear/immunology ; Leukocytes, Mononuclear/microbiology ; Lung/immunology ; Lung/microbiology ; Male ; Middle Aged ; Mycobacterium tuberculosis/immunology ; Programmed Cell Death 1 Receptor/immunology ; Sputum/microbiology ; T-Lymphocytes, Helper-Inducer/immunology ; Tuberculosis, Multidrug-Resistant/immunology ; Tuberculosis, Multidrug-Resistant/microbiology ; Tuberculosis, Pulmonary/immunology ; Tuberculosis, Pulmonary/microbiology ; Young Adult ; Interleukin-22
Chemical Substances	Antigens, Bacterial ; CD57 Antigens ; Interleukin-17 ; Interleukins ; Programmed Cell Death 1 Receptor ; Interferon-gamma (82115-62-6)
Language	English
Publishing date	2020-11-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	218531-3
ISSN	1365-2249 ; 0009-9104 ; 0964-2536
ISSN (online)	1365-2249
ISSN	0009-9104 ; 0964-2536
DOI	10.1111/cei.13544
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Article ; Online: Effect of chemical modulation of toll-like receptor 4 in an animal model of ulcerative colitis.

Facchini, Fabio Alessandro / Di Fusco, Davide / Barresi, Simona / Luraghi, Andrea / Minotti, Alberto / Granucci, Francesca / Monteleone, Giovanni / Peri, Francesco / Monteleone, Ivan

European journal of clinical pharmacology

2020 Volume 76, Issue 3, Page(s) 409–418

Abstract: Purpose: The partial ineffectiveness and side effects of inflammatory bowel disease (IBD) current therapies drive basic research to look for new therapeutic target in order to develop new drug lead. Considering the pivotal role played by toll-like ... ...

Abstract	Purpose: The partial ineffectiveness and side effects of inflammatory bowel disease (IBD) current therapies drive basic research to look for new therapeutic target in order to develop new drug lead. Considering the pivotal role played by toll-like receptors (TLRs) in gut inflammation, we evaluate here the therapeutic effect of the synthetic glycolipid TLR4 antagonist FP7. Methods: The anti-inflammatory effect of FP7, active as TLR4 antagonist, was evaluated on peripheral blood mononuclear cells (PBMCs) and lamina propria mononuclear cells (LPMCs) isolated from IBD patients, and in a mouse model of ulcerative colitis. Results: FP7 strongly reduced the inflammatory responses induced by lipopolysaccharide (LPS) in vitro, due to its capacity to compete with LPS for the binding of TLR4/MD-2 receptor complex thus inhibiting both the MyD88- and TRIF-dependent inflammatory pathways. Colitic mice treated with FP7 exhibit reduced colonic inflammation and decreased levels of pro-inflammatory cytokines. Conclusions: This study suggests that TLR4 chemical modulation can be an effective therapeutic approach to IBD. The selectivity of FP7 on TLR4 makes this molecule a promising drug lead for new small molecules-based treatments.
MeSH term(s)	Adult ; Animals ; Cells, Cultured ; Colitis, Ulcerative/drug therapy ; Colitis, Ulcerative/metabolism ; Colon/drug effects ; Colon/metabolism ; Cytokines/metabolism ; Disease Models, Animal ; Female ; Glycolipids/therapeutic use ; Humans ; Inflammation/drug therapy ; Inflammation/metabolism ; Inflammatory Bowel Diseases/drug therapy ; Inflammatory Bowel Diseases/metabolism ; Intestinal Mucosa/drug effects ; Intestinal Mucosa/metabolism ; Leukocytes, Mononuclear/drug effects ; Leukocytes, Mononuclear/metabolism ; Male ; Mice ; Middle Aged ; Toll-Like Receptor 4/metabolism ; Young Adult
Chemical Substances	Cytokines ; Glycolipids ; TLR4 antagonist FP7 ; TLR4 protein, human ; Toll-Like Receptor 4
Language	English
Publishing date	2020-01-25
Publishing country	Germany
Document type	Journal Article
ZDB-ID	121960-1
ISSN	1432-1041 ; 0031-6970
ISSN (online)	1432-1041
ISSN	0031-6970
DOI	10.1007/s00228-019-02799-7
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Article ; Online: Psychological Profile in Coronary Artery By-Pass Graft Patients vs. Valve Replacement Patients Entering Cardiac Rehabilitation after Surgery.

Modica, Maddalena / Castiglioni, Paolo / Minotti, Anna / Faini, Andrea / Racca, Vittorio / Ferratini, Maurizio

Scientific reports

2018 Volume 8, Issue 1, Page(s) 14381

Abstract: Anxiety and depression are thought to influence the genesis of ischemic diseases and not of valvular diseases, but little is known on the psychological profile of cardiac patients after surgery. Aim of this study was to investigate differences in disease ...

Abstract	Anxiety and depression are thought to influence the genesis of ischemic diseases and not of valvular diseases, but little is known on the psychological profile of cardiac patients after surgery. Aim of this study was to investigate differences in disease experience and mood between patients undergoing cardiac rehabilitation after coronary artery by-pass graft (CABG) or after valve replacement (VR). We studied 1,179 CABG and 737 VR patients who completed the Illness Behaviour Questionnaire and the Hospital Anxiety and Depression Scale after surgery. We tested the independent effect of the type of surgery by multivariate analysis and between-group differences in prevalence of clinically relevant scores. Relevant scores in the psychosomatic concern scale were more frequent in CABG than in VR patients. After correction by age, sex, education and marital status, scores of disease conviction and psychosomatic concern were higher in CABG patients, scores of denial were higher in VR patients. Unexpectedly, anxiety and depression scores did not differ between groups. Results suggest providing psychological support for anxiety and depression to both VR and CABG patients during cardiac rehabilitation, and planning differentiated interventions of cardiac rehabilitation and secondary prevention tailored to the specific psychological reactions of CABG and VR patients.
MeSH term(s)	Affect ; Aged ; Anxiety/etiology ; Anxiety/psychology ; Cardiac Rehabilitation/psychology ; Coronary Artery Bypass/psychology ; Depression/etiology ; Depression/psychology ; Female ; Heart Valve Prosthesis ; Heart Valve Prosthesis Implantation/psychology ; Heart Valves/surgery ; Humans ; Male ; Middle Aged
Language	English
Publishing date	2018-09-26
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-018-32696-5
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Article ; Online: Coping, Mood, Quality of Life, and Outcomes in Recipients of Left Ventricular Assist Devices: A Cluster Analysis.

Modica, Maddalena / Minotti, Anna / De Maria, Renata / Scaglione, Anna / Bordoni, Bruno / Cipriani, Manlio / Russo, Claudio / Racca, Vittorio / Ferratini, Maurizio

Psychosomatic medicine

2019 Volume 81, Issue 2, Page(s) 192–199

Abstract: Objective: Left ventricular assist devices (LVADs) are increasingly used for temporary circulatory support until transplant or as destination therapy for patients with end-stage heart failure. Understanding patients' attitudes and resources is crucial ... ...

Abstract	Objective: Left ventricular assist devices (LVADs) are increasingly used for temporary circulatory support until transplant or as destination therapy for patients with end-stage heart failure. Understanding patients' attitudes and resources is crucial to support them. Methods: Sixty-one LVAD recipients (55 [10] years, 10% women, 15% destination therapy) participating in cardiac rehabilitation (CR) postimplant underwent assessment of coping styles (Coping Orientation for Problem Experiences), quality of life (Medical Outcomes Study Short-Form 36, Minnesota Living with Heart Failure Questionnaire), and mood (Hospital Anxiety and Depression Scale). Results: Cluster analysis identified two homogeneous groups: C1 (n = 18) patients had higher anxiety (9.6 [5.0] versus 4.0 [2.7], p < .001), depression (10 [3.0] versus 4 [2.7], p < .001), and avoidant coping (29 [5.2] versus 21.2 [3.8], p < .001) scores and worse quality of life (Short-Form 36 of the Medical Outcomes Study Physical Component Scale 31 [5.3] versus 35.6 [6.9], p < .001; Mental Component Scale 34.3 [6.8] versus 50.3 [8], p < .001; MLHFQ physical 26 [7.2] versus 13.9 [8.2], p < .001; emotional 17.7 [3.4] versus 3.86 [3.2], p < .001) than C2 participants (n = 43). During 31 (15-54) months, 20 patients (33%) died. By Cox multivariable analysis, after adjustment for age, sex, Interagency Registry for Mechanically Assisted Circulatory Support level at surgery, and meters walked during 6 minutes at CR discharge, C1 was associated with mortality (hazard ratio = 2.858; 95% confidence interval = 1.102-7.408, p = .031; model χ = 7.286, df = 5, p = .20). Survival was 44% in C1 and 77% in C2 (log-rank p = .033). Conclusions: Among patients discharged from CR after LVAD implant, one third show a profile characterized by worse perceived quality of life, mood disorders, and dysfunctional coping that is associated with long-term mortality, and these factors are potential targets for post-LVAD intensive support.
MeSH term(s)	Adaptation, Psychological/physiology ; Anxiety/psychology ; Cluster Analysis ; Depression/psychology ; Female ; Heart Failure/psychology ; Heart Failure/therapy ; Heart Ventricles ; Heart-Assist Devices ; Humans ; Male ; Middle Aged ; Quality of Life/psychology ; Treatment Outcome
Language	English
Publishing date	2019-01-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3469-1
ISSN	1534-7796 ; 0033-3174
ISSN (online)	1534-7796
ISSN	0033-3174
DOI	10.1097/PSY.0000000000000658
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Article: Flexible PVDF-TrFE pyroelectric sensor driven by polysilicon thin film transistor fabricated on ultra-thin polyimide substrate

Pecora, A / Maiolo, L / Maita, F / Minotti, A

Sensors & Actuators: A. Physical. 2012 Oct., v. 185

2012

Abstract: In this work we present a flexible pyroelectric sensor composed by a PVDF-TrFE capacitor realized on ultra-thin polyimide film (5μm thick), integrated with a n-channel low temperature polysilicon thin film transistor also fabricated on ultra-thin ... ...

Abstract	In this work we present a flexible pyroelectric sensor composed by a PVDF-TrFE capacitor realized on ultra-thin polyimide film (5μm thick), integrated with a n-channel low temperature polysilicon thin film transistor also fabricated on ultra-thin polyimide (8μm thick). Exploiting a multi-foil approach, the pyroelectric capacitors and the transistors were attached one over the other reaching a final thickness of about 15μm. The bottom contact of the sensor capacitance was connected to the gate of the transistor by a silver ink, while, for bias and load resistances, we used external elements. The active sensor area was defined by a circular capacitor with a diameter of about 2mm. In order to enhance PVDF-TrFE pyroelectric properties, an external stepwise voltage was applied to the structure up to values of 160V at a temperature of about 80°C. The devices were then tested, at different working frequencies (up to 800Hz) under a specific infrared radiation provided by a He–Ne laser, with a wavelength of 632nm and maximum power of 5mW. An output signal of tens of millivolt was observed at 10Hz, exploiting the pre-amplification of polysilicon thin film transistor.
Keywords	capacitance ; electrical equipment ; infrared radiation ; silver ; temperature ; wavelengths
Language	English
Dates of publication	2012-10
Size	p. 39-43.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1500729-7
ISSN	0924-4247
ISSN	0924-4247
DOI	10.1016/j.sna.2012.07.013
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Co-administration of Antimicrobial Peptides Enhances Toll-like Receptor 4 Antagonist Activity of a Synthetic Glycolipid.

Facchini, Fabio A / Coelho, Helena / Sestito, Stefania E / Delgado, Sandra / Minotti, Alberto / Andreu, David / Jiménez-Barbero, Jesús / Peri, Francesco

ChemMedChem

2018 Volume 13, Issue 3, Page(s) 280–287

Abstract: This study examines the effect of co-administration of antimicrobial peptides and the synthetic glycolipid FP7, which is active in inhibiting inflammatory cytokine production caused by TLR4 activation and signaling. The co-administration of two ... ...

Abstract	This study examines the effect of co-administration of antimicrobial peptides and the synthetic glycolipid FP7, which is active in inhibiting inflammatory cytokine production caused by TLR4 activation and signaling. The co-administration of two lipopolysaccharide (LPS)-neutralizing peptides (a cecropin A-melittin hybrid peptide and a human cathelicidin) enhances by an order of magnitude the potency of FP7 in blocking the TLR4 signal. Interestingly, this is not an additional effect of LPS neutralization by peptides, because it also occurs if cells are stimulated by the plant lectin phytohemagglutinin, a non-LPS TLR4 agonist. Our data suggest a dual mechanism of action for the peptides, not exclusively based on LPS binding and neutralization, but also on a direct effect on the LPS-binding proteins of the TLR4 receptor complex. NMR experiments in solution show that peptide addition changes the aggregation state of FP7, promoting the formation of larger micelles. These results suggest a relationship between the aggregation state of lipid A-like ligands and the type and intensity of the TLR4 response.
MeSH term(s)	Acute-Phase Proteins/metabolism ; Antimicrobial Cationic Peptides/chemistry ; Antimicrobial Cationic Peptides/pharmacology ; Carrier Proteins/metabolism ; Cell Line ; Cell Survival/drug effects ; Glycolipids/chemistry ; Glycolipids/pharmacology ; Humans ; Ligands ; Melitten/chemistry ; Melitten/pharmacology ; Membrane Glycoproteins/metabolism ; Protein Binding ; Protein Conformation ; Toll-Like Receptor 4/antagonists & inhibitors ; Toll-Like Receptor 4/metabolism
Chemical Substances	Acute-Phase Proteins ; Antimicrobial Cationic Peptides ; Carrier Proteins ; Glycolipids ; Ligands ; Membrane Glycoproteins ; TLR4 protein, human ; Toll-Like Receptor 4 ; lipopolysaccharide-binding protein ; Melitten (20449-79-0) ; ropocamptide (3DD771JO2H) ; cecropin A (80451-04-3)
Language	English
Publishing date	2018-01-24
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2218496-X
ISSN	1860-7187 ; 1860-7179
ISSN (online)	1860-7187
ISSN	1860-7179
DOI	10.1002/cmdc.201700694
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