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  1. Article ; Online: Loss of the sustained antidepressant-like effect of (2R,6R)-hydroxynorketamine in NMDA receptor GluN2D subunit knockout mice.

    Yamagishi, Aimi / Ikekubo, Yuiko / Mishina, Masayoshi / Ikeda, Kazutaka / Ide, Soichiro

    Journal of pharmacological sciences

    2024  Volume 154, Issue 3, Page(s) 203–208

    Abstract: Ketamine, an N-methyl-d-aspartate (NMDA) receptor antagonist, has attracted attention for its acute and sustained antidepressant effects in patients with depression. Hydroxynorketamine (HNK), a metabolite of ketamine, exerts antidepressant effects ... ...

    Abstract Ketamine, an N-methyl-d-aspartate (NMDA) receptor antagonist, has attracted attention for its acute and sustained antidepressant effects in patients with depression. Hydroxynorketamine (HNK), a metabolite of ketamine, exerts antidepressant effects without exerting ketamine's side effects and has attracted much attention in recent years. However, the detailed pharmacological mechanism of action of HNK remains unclear. We previously showed that the GluN2D NMDA receptor subunit is important for sustained antidepressant-like effects of (R)-ketamine. Therefore, we investigated whether the GluN2D subunit is involved in antidepressant-like effects of (2R,6R)-HNK and (2S,6S)-HNK. Treatment with (2R,6R)-HNK but not (2S,6S)-HNK exerted acute and sustained antidepressant-like effects in the tail-suspension test in wildtype mice. Interestingly, sustained antidepressant-like effects of (2R,6R)-HNK were abolished in GluN2D-knockout mice, whereas acute antidepressant-like effects were maintained in GluN2D-knockout mice. When expression levels of GluN2A and GluN2B subunits were evaluated, a decrease in GluN2B protein expression in the nucleus accumbens was found in stressed wildtype mice but not in stressed GluN2D-knockout mice. These results suggest that the GluN2D subunit and possibly the GluN2B subunit are involved in the sustained antidepressant-like effect of (2R,6R)-HNK.
    MeSH term(s) Humans ; Mice ; Animals ; Ketamine/pharmacology ; Ketamine/metabolism ; Ketamine/analogs & derivatives ; Depression/drug therapy ; Depression/genetics ; Receptors, N-Methyl-D-Aspartate/metabolism ; Mice, Knockout ; Antidepressive Agents/pharmacology
    Chemical Substances 6-hydroxynorketamine (81395-70-2) ; Ketamine (690G0D6V8H) ; Receptors, N-Methyl-D-Aspartate ; Antidepressive Agents
    Language English
    Publishing date 2024-01-26
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2104264-0
    ISSN 1347-8648 ; 1347-8613
    ISSN (online) 1347-8648
    ISSN 1347-8613
    DOI 10.1016/j.jphs.2024.01.008
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  2. Article ; Online: Bilateral Cortical-sparing Adrenalectomy for the Treatment of Bilateral Aldosterone-producing Adenomas.

    Nanba, Kazutaka / Kaneko, Hiroki / Mishina, Mutsuki / Tagami, Tetsuya

    JCEM case reports

    2023  Volume 1, Issue 6, Page(s) luad144

    Abstract: Aldosterone-producing adenoma (APA) is 1 of the major subtypes of primary aldosteronism (PA). Although most APA occurs unilaterally, bilateral APAs have rarely been documented. Because of its rarity, optimal management of patients with bilateral APAs has ...

    Abstract Aldosterone-producing adenoma (APA) is 1 of the major subtypes of primary aldosteronism (PA). Although most APA occurs unilaterally, bilateral APAs have rarely been documented. Because of its rarity, optimal management of patients with bilateral APAs has not been established. Here, we report a case of bilateral APAs that was successfully treated with simultaneous bilateral cortical-sparing surgery. A 44-year-old Japanese woman was referred to us for the evaluation of PA. She had typical clinical characteristics of PA, including hypertension, hypokalemia, and high plasma aldosterone concentration with suppressed renin. She was diagnosed as having PA based on the results of confirmatory testing. Computed tomography revealed bilateral adrenal nodules with diameters of 17 and 10 mm on the right and left adrenal gland, respectively. Adrenal venous sampling indicated excess aldosterone production from bilateral adrenal lesions. She underwent simultaneous bilateral laparoscopic partial adrenalectomy that normalized her blood pressure and serum potassium levels. Aldosterone synthase immunohistochemistry on the resected adrenal tumor tissue confirmed the diagnosis of bilateral APAs. Long-term postsurgical follow-up data suggest cure of the disease without the need for glucocorticoid replacement therapy. Bilateral cortical-sparing adrenalectomy appears to be a viable treatment option at least for selected patients with bilateral APAs.
    Language English
    Publishing date 2023-12-07
    Publishing country England
    Document type Case Reports
    ISSN 2755-1520
    ISSN (online) 2755-1520
    DOI 10.1210/jcemcr/luad144
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  3. Article ; Online: Correction to: Generative image transformer (GIT): unsupervised continuous image generative and transformable model for [

    Watanabe, Shogo / Ueno, Tomohiro / Kimura, Yuichi / Mishina, Masahiro / Sugimoto, Naozo

    Annals of nuclear medicine

    2022  Volume 36, Issue 4, Page(s) 427

    Language English
    Publishing date 2022-01-06
    Publishing country Japan
    Document type Published Erratum
    ZDB-ID 1146984-5
    ISSN 1864-6433 ; 0914-7187
    ISSN (online) 1864-6433
    ISSN 0914-7187
    DOI 10.1007/s12149-021-01714-4
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  4. Article ; Online: Genetically fused charged peptides induce rapid crystallization of proteins.

    Minamihata, K / Tsukamoto, K / Adachi, M / Shimizu, R / Mishina, M / Kuroki, R / Nagamune, T

    Chemical communications (Cambridge, England)

    2020  Volume 56, Issue 27, Page(s) 3891–3894

    Abstract: We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We ... ...

    Abstract We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials.
    MeSH term(s) Biotin/analogs & derivatives ; Biotin/chemistry ; Biotinylation ; Crystallization ; DNA/chemistry ; Dendrimers/chemistry ; Fluoresceins/chemistry ; Fluorescent Dyes/chemistry ; Peptides/chemistry ; Static Electricity ; Streptavidin/chemistry
    Chemical Substances Dendrimers ; Fluoresceins ; Fluorescent Dyes ; PAMAM Starburst ; Peptides ; biotin-4-fluorescein ; Biotin (6SO6U10H04) ; DNA (9007-49-2) ; Streptavidin (9013-20-1)
    Language English
    Publishing date 2020-03-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/c9cc09529b
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  5. Article ; Online: Generative image transformer (GIT): unsupervised continuous image generative and transformable model for [

    Watanabe, Shogo / Ueno, Tomohiro / Kimura, Yuichi / Mishina, Masahiro / Sugimoto, Naozo

    Annals of nuclear medicine

    2021  Volume 35, Issue 11, Page(s) 1203–1213

    Abstract: Objective: Recently, generative adversarial networks began to be actively studied in the field of medical imaging. These models are used for augmenting the variation of images to improve the accuracy of computer-aided diagnosis. In this paper, we ... ...

    Abstract Objective: Recently, generative adversarial networks began to be actively studied in the field of medical imaging. These models are used for augmenting the variation of images to improve the accuracy of computer-aided diagnosis. In this paper, we propose an alternative new image generative model based on transformer decoder blocks and verify the performance of our model in generating SPECT images that have characteristics of Parkinson's disease patients.
    Methods: Firstly, we proposed a new model architecture that is based on a transformer decoder block and is extended to generate slice images. From few superior slices of 3D volume, our model generates the rest of the inferior slices sequentially. Our model was trained by using [
    Results: Our model was successfully generated and transformed into PD-like SPECT images. The mean absolute SNBR was mostly less than 0.15 in absolute value. The variation of the obtained dataset images was confirmed by the analysis of the asymmetric index.
    Conclusions: These results showed the potential ability of our new generative approach for SPECT images that the generative model based on the transformer realized both generation and transformation by a single model.
    MeSH term(s) Tomography, Emission-Computed, Single-Photon ; Tropanes
    Chemical Substances Tropanes ; 2-carbomethoxy-8-(3-fluoropropyl)-3-(4-iodophenyl)tropane (155797-99-2)
    Language English
    Publishing date 2021-08-04
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 1146984-5
    ISSN 1864-6433 ; 0914-7187
    ISSN (online) 1864-6433
    ISSN 0914-7187
    DOI 10.1007/s12149-021-01661-0
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  6. Article: [Evaluation of Patients with Localized Prostate Cancer in whom Long-Term Successful Androgen Deprivation Therapy was Ceased].

    Araki, Hiromasa / Ito, Katsuhiro / Igarashi, Atsushi / Miura, Takayoshi / Miyazaki, Yu / Mishina, Mutsuki / Okuno, Hiroshi

    Hinyokika kiyo. Acta urologica Japonica

    2022  Volume 68, Issue 11, Page(s) 337–343

    Abstract: We evaluated the clinical course of patients with localized prostate cancer in whom long-term successful androgen deprivation therapy (ADT) was ceased. Study subjects were 24 patients with stage B prostate cancer who were initially treated with ADT for a ...

    Abstract We evaluated the clinical course of patients with localized prostate cancer in whom long-term successful androgen deprivation therapy (ADT) was ceased. Study subjects were 24 patients with stage B prostate cancer who were initially treated with ADT for a median duration of 93 months. The median age at the cessation of ADT was 84 years. The median nadir serum prostate specific antigen (PSA) level was 0.022 ng/ml. The median duration of follow-up from the cessation of ADT was 31 months. During follow-up, five patients showed PSA elevation of ≥2 ng/ml from the nadir. Serum testosterone level was tested in 20 patients, and five showed testosterone recovery ≥0.5 ng/ml. Seven patients died from diseases other than prostate cancer, but there were no deaths caused by prostate cancer. This study demonstrated that long-term successful ADT for localized prostate cancer could be ceased with adequate follow-up evaluation.
    MeSH term(s) Male ; Humans ; Aged, 80 and over ; Androgen Antagonists/therapeutic use ; Androgens ; Prostate-Specific Antigen ; Prostatic Neoplasms/drug therapy ; Testosterone
    Chemical Substances Androgen Antagonists ; Androgens ; Prostate-Specific Antigen (EC 3.4.21.77) ; Testosterone (3XMK78S47O)
    Language Japanese
    Publishing date 2022-09-30
    Publishing country Japan
    Document type English Abstract ; Journal Article
    ZDB-ID 604331-8
    ISSN 0018-1994
    ISSN 0018-1994
    DOI 10.14989/ActaUrolJap_68_11_337
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  7. Article ; Online: EFFECT OF ULTRASOUND RADIATION ON FORMED BIOFILMS AND ABILITY TO THEIR FORMATION IN S.AUREUS

    Mishina M.M.

    Anali Mečnikìvsʹkogo Institutu, Vol 4, Pp 33-

    2013  Volume 36

    Abstract: Microbiological research of the clinical material receivedfrom with inflammatory processes was carried on that todetect ability to form biofilms and to study effect of low-intensity ultrasound radiation on formed biofilms and theiraggregation ability. ... ...

    Abstract Microbiological research of the clinical material receivedfrom with inflammatory processes was carried on that todetect ability to form biofilms and to study effect of low-intensity ultrasound radiation on formed biofilms and theiraggregation ability. Performed research showed that ultrasound radiation of low intensity could destroy biofilms and inhibit ability of microorganisms to form secondary biofilms.
    Keywords ultrasound radiation ; biofilms ; S.aureus ; Medicine (General) ; R5-920
    Language English
    Publishing date 2013-12-01T00:00:00Z
    Publisher Mechnikov Institute of Microbiology and immunology
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Kinesiophobia, self-reported ankle function, and sex are associated with perceived ankle instability in college club sports athletes with chronic ankle instability.

    Watanabe, Kentaro / Koshino, Yuta / Kawahara, Daiki / Akimoto, Moeko / Mishina, Masumi / Nakagawa, Kosumi / Ishida, Tomoya / Kasahara, Satoshi / Samukawa, Mina / Tohyama, Harukazu

    Physical therapy in sport : official journal of the Association of Chartered Physiotherapists in Sports Medicine

    2023  Volume 61, Page(s) 45–50

    Abstract: Objective: To investigate the association between sex, self-reported ankle function, pain intensity, kinesiophobia, and perceived ankle instability in athletes with chronic ankle instability (CAI).: Design: Cross-Sectional Study.: Setting: ... ...

    Abstract Objective: To investigate the association between sex, self-reported ankle function, pain intensity, kinesiophobia, and perceived ankle instability in athletes with chronic ankle instability (CAI).
    Design: Cross-Sectional Study.
    Setting: University.
    Participants: College club sports athletes with CAI (n = 42).
    Main outcome measures: Relationships with the Cumberland Ankle Instability Tool (CAIT) score and the Tampa Scale for Kinesiophobia-11 (TSK-11), the Foot and Ankle Ability Measure (FAAM), sex (0: male, 1: female), and ankle pain intensity by the Numeric Rating Scale were explored with multiple regression analysis.
    Results: The regression model explained 50.3% of the variance of the CAIT score (P < 0.001), and the TSK-11 score (B = -0.382, P = 0.002), the FAAM sports subscale score (B = 0.122, P = 0.038), and sex (B = -2.646, P = 0.031) were significant independent variables for the CAIT score (P < 0.001), while pain intensity was not significant (B = -0.182, P = 0.504). These results indicated that higher TSK-11 score, lower FAAM sports subscale score, and being female were related to lower CAIT score.
    Conclusions: Kinesiophobia related to perceived instability along with self-reported function and sex in athletes with CAI. Clinicians should assess the psychological aspects of athletes with CAI.
    MeSH term(s) Humans ; Male ; Female ; Ankle ; Self Report ; Cross-Sectional Studies ; Kinesiophobia ; Ankle Injuries ; Ankle Joint ; Athletes ; Joint Instability ; Arthralgia ; Chronic Disease
    Language English
    Publishing date 2023-02-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 2008604-0
    ISSN 1873-1600 ; 1466-853X
    ISSN (online) 1873-1600
    ISSN 1466-853X
    DOI 10.1016/j.ptsp.2023.02.008
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    Zs.A 5218: Show issues Location:
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  9. Article ; Online: The limit of detection of the BioFire® FilmArray® gastrointestinal panel for the foodborne parasite Cyclospora cayetanensis.

    Peterson, Anna / Richins, Travis / Houghton, Katelyn / Mishina, Margarita / Sharma, Suresh / Sambhara, Suryaprakash / Jacobson, David / Qvarnstrom, Yvonne / Cama, Vitaliano

    Diagnostic microbiology and infectious disease

    2023  Volume 107, Issue 2, Page(s) 116030

    Abstract: Cyclosporiasis is a foodborne diarrheal illness caused by the parasite Cyclospora cayetanensis. The BioFire® FilmArray® gastrointestinal (FilmArray GI) panel is a common method for diagnosing cyclosporiasis from clinical stool samples. The currently ... ...

    Abstract Cyclosporiasis is a foodborne diarrheal illness caused by the parasite Cyclospora cayetanensis. The BioFire® FilmArray® gastrointestinal (FilmArray GI) panel is a common method for diagnosing cyclosporiasis from clinical stool samples. The currently published limit of detection (LOD) of this panel is in genome equivalents; however, it is unclear how this relates to the number of C. cayetanensis oocysts in a clinical sample. In this study, we developed a technique to determine the LOD in terms of oocysts, using a cell sorter to sort 1 to 50 C. cayetanensis oocyst(s) previously purified from three human stool sources. We found the FilmArray GI panel detected samples with ≥20 C. cayetanensis oocysts in 100% of replicates, with varying detection among samples with 1, 5, or 10 C. cayetanensis oocysts. This method provides a parasitologically relevant LOD that should enable comparison among C. cayetanensis detection techniques, including the FilmArray GI panel.
    MeSH term(s) Animals ; Humans ; Cyclospora/genetics ; Cyclosporiasis/diagnosis ; Cyclosporiasis/parasitology ; Parasites ; Limit of Detection ; Feces/parasitology ; Oocysts/genetics
    Language English
    Publishing date 2023-07-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604920-5
    ISSN 1879-0070 ; 0732-8893
    ISSN (online) 1879-0070
    ISSN 0732-8893
    DOI 10.1016/j.diagmicrobio.2023.116030
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  10. Article ; Online: Limb-Clasping Response in NMDA Receptor Palmitoylation-Deficient Mice.

    Suzuki, Nami / Oota-Ishigaki, Akiko / Kaizuka, Toshie / Itoh, Masayuki / Yamazaki, Maya / Natsume, Rie / Abe, Manabu / Sakimura, Kenji / Mishina, Masayoshi / Hayashi, Takashi

    Molecular neurobiology

    2024  

    Abstract: Proper regulation of N-methyl-D-aspartate-type glutamate receptor (NMDA receptor) expression is responsible for excitatory synaptic functions in the mammalian brain. NMDA receptor dysfunction can cause various neuropsychiatric disorders and ... ...

    Abstract Proper regulation of N-methyl-D-aspartate-type glutamate receptor (NMDA receptor) expression is responsible for excitatory synaptic functions in the mammalian brain. NMDA receptor dysfunction can cause various neuropsychiatric disorders and neurodegenerative diseases. Posttranslational protein S-palmitoylation, the covalent attachment of palmitic acid to intracellular cysteine residues via thioester bonds, occurs in the carboxyl terminus of GluN2B, which is the major regulatory NMDA receptor subunit. Mutations of three palmitoylatable cysteine residues in the membrane-proximal cluster of GluN2B to non-palmitoylatable serine (3CS) lead to the dephosphorylation of GluN2B Tyr1472 in the hippocampus and cerebral cortex, inducing a reduction in the surface expression of GluN2B-containig NMDA receptors. Furthermore, adult GluN2B 3CS homozygous mice demonstrated a definite clasping response without abnormalities in the gross brain structure, other neurological reflexes, or expression levels of synaptic proteins in the cerebrum. This behavioral disorder, observed in the GluN2B 3CS knock-in mice, indicated that complex higher brain functions are coordinated through the palmitoylation-dependent regulation of NMDA receptors in excitatory synapses.
    Language English
    Publishing date 2024-04-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 645020-9
    ISSN 1559-1182 ; 0893-7648
    ISSN (online) 1559-1182
    ISSN 0893-7648
    DOI 10.1007/s12035-024-04166-9
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