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Article ; Online: Extraction of selected minerals from Melissa officinalis L. leaves by using two-level factorial design

Mitić Jelena / Mitić Milan

Kragujevac Journal of Science, Vol 2023, Iss 45, Pp 51-

2023 Volume 64

Abstract: In this study, a two-level factorial design was used to evaluate the significant extraction parameters in achieving higher recovery yield of Cu, Fe, Mn, and Zn contents from Melissa officinalis leaves. The independent parameters were solid-to-solvent ... ...

Abstract	In this study, a two-level factorial design was used to evaluate the significant extraction parameters in achieving higher recovery yield of Cu, Fe, Mn, and Zn contents from Melissa officinalis leaves. The independent parameters were solid-to-solvent ratio, extraction temperature, and duration of extraction. The experimental data obtained were fitted to a first-order polynomial equation using multiple regression analysis and studied by appropriate statistical methods (ANOVA). The optimum extraction conditions were as follows: solid-to-solvent ratio 1:15 g/mL, extraction temperature 100°C, and extraction time 80 min. Under these conditions, the experimental concentrations were in close agreement with the value predicted by the model. The correlation coefficients of 0.9947, 0.9966, 0.9939, and 0.9957 observed between the predicted and actual values for the response variables are evidence that the regression model can represent the experimental data well. Minerals present in the extracts may be maximized when process conditions are carefully adjusted within the reported values.
Keywords	extraction ; lemon balm ; factorial design ; minerals ; Science ; Q
Subject code	660
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	University of Kragujevac, Faculty of Science
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Identification of synthetic cannabinoid methyl 2-{[1-(cyclohexylmethyl)-1H-indol-3-yl] formamido}-3-methylbutanoate using modern mass spectrometry and nuclear magnetic resonance techniques

Lukic Vera / Micic Ruzica / Arsic Biljana / Mitic Milan / Jovanovic Milos / Pavlovic Aleksandra

Open Chemistry, Vol 19, Iss 1, Pp 1259-

2021 Volume 1273

Keywords	mmb-chmica ; synthetic cannabinoids ; identification of nps ; high-resolution mass spectrometry ; mrm optimization ; Chemistry ; QD1-999
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	De Gruyter
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: In Silico Investigation of Selected Pesticides and Their Determination in Agricultural Products Using QuEChERS Methodology and HPLC-DAD.

Petrović, Stefan / Arsić, Biljana / Zlatanović, Ivana / Milićević, Jelena / Glišić, Sanja / Mitić, Milan / Đurović-Pejčev, Rada / Stojanović, Gordana

International journal of molecular sciences

2023 Volume 24, Issue 9

Abstract: In this study, we considered some pesticides as active substances within formulations for the protection of plant-based food in the Republic of Serbia in silico, because these pesticides have not often been investigated in this way previously, and in an ... ...

Abstract	In this study, we considered some pesticides as active substances within formulations for the protection of plant-based food in the Republic of Serbia in silico, because these pesticides have not often been investigated in this way previously, and in an analytical way, because there are not very many available fast, cheap, and easy methods for their determination in real agricultural samples. Seven pesticides were detected in selected agricultural products (tomatoes, cucumbers, peppers, and grapes) using the QuEChERS methodology and HPLC-DAD. Standard curves for the investigated pesticides (chlorantraniliprole, methomyl, metalaxyl, thiacloprid, acetamiprid, emamectin benzoate, and cymoxanil) show good linearity, with R
MeSH term(s)	Humans ; Pesticides/chemistry ; Chromatography, High Pressure Liquid/methods ; Molecular Docking Simulation ; ortho-Aminobenzoates ; Pesticide Residues/analysis
Chemical Substances	Pesticides ; chlorantranilipole (622AK9DH9G) ; ortho-Aminobenzoates ; Pesticide Residues
Language	English
Publishing date	2023-04-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24098003
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Anticancer assessment and antibiofilm potential of

Jovanović, Milena M / Marković, Katarina G / Grujović, Mirjana Ž / Pavić, Jelena / Mitić, Milan / Nikolić, Jelena / Šeklić, Dragana

Food science & nutrition

2023 Volume 11, Issue 10, Page(s) 6393–6402

Abstract: Laetiporus ... ...

Abstract	Laetiporus sulphureus
Language	English
Publishing date	2023-07-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	2703010-6
ISSN	2048-7177
ISSN	2048-7177
DOI	10.1002/fsn3.3577
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Article ; Online: Kinetic models of the extraction of vanillic acid from pumpkin seeds

Mitić Milan / Janković Sonja / Mašković Pavle / Arsić Biljana / Mitić Jelena / Ickovski Jovana

Open Chemistry, Vol 18, Iss 1, Pp 22-

2020 Volume 30

Abstract: Vanillic acid is used in the food industry and perfumery, and the optimization of its extraction process from the natural source is important for saving time and money. The presence of vanillic acid in pumpkin seeds was proven using HPLC analysis. ... ...

Abstract	Vanillic acid is used in the food industry and perfumery, and the optimization of its extraction process from the natural source is important for saving time and money. The presence of vanillic acid in pumpkin seeds was proven using HPLC analysis. Computational optimization of the extraction shows that for the concentration of ethanol 40% and solmodul: V/m=20, the optimum condition for the extraction of vanillic acid from pumpkin seeds was 100 min and 450C. The estimation of fitting for each kinetic model to the experimental kinetic data was performed using the root mean square, standard deviation, and the correlation coefficient. Ponomarev model was shown as the most suitable with the highest accuracy among the six considered kinetic models. The enthalpy and entropy changes were positive, while the Gibbs free energy was negative and decreased when temperature increased during the thermodynamic analysis. Therefore, the extraction of vanillic acid from pumpkin seeds was endothermic, spontaneous, and irreversible.
Keywords	extraction yield ; vanillic acid ; pumpkin seed ; ponomarev model ; Chemistry ; QD1-999
Subject code	660
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	De Gruyter
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Chemometric Approach to the Composition of Flavonoid Compounds and Phenolic Acids and Antioxidant Potential of Artemisia Species from Different Habitats.

Ickovski, Jovana D / Arsić, Biljana B / Mitić, Milan N / Stojković, Milan B / Đorđević, Milan M / Stojanović, Gordana S

Chemistry & biodiversity

2022 Volume 19, Issue 12, Page(s) e202200365

Abstract: Four Artemisia species from Serbia were selected for the study: A. annua L., A. absinthium L., A. vulgaris L. and A. scoparia Waldst. et Kit. because of the proven excellent action in treating certain medical conditions and diseases. A. absinthium L., A. ...

Abstract	Four Artemisia species from Serbia were selected for the study: A. annua L., A. absinthium L., A. vulgaris L. and A. scoparia Waldst. et Kit. because of the proven excellent action in treating certain medical conditions and diseases. A. absinthium L., A. vulgaris L., A. annua L. and A. scoparia Waldst. et Kit. collected from different habitats across Serbia (48 samples in total) were studied from the statistical aspect considering the phenolic and flavonoid contents, compositions, and antioxidant activities of methanol extracts in correlation with the soil type. The components were identified using HPLC (High Performance Liquid Chromatography), while antioxidant activities were determined by seven assays (TP (Total phenolic content), TF (Total flavonoid content), DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-based, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical cation-based, FRAP (Ferric Reducing Antioxidant Power), TRP (Total Reducing Power), and CUPRAC (Cupric Reducing Antioxidant Capacity). The results were processed using five statistical methods (PCA (Principal Component Analysis), ANOVA (Analysis of Variance), MANOVA (Multivariate analysis of variance), DA (Discriminant analysis), and AHC (Agglomerative Hierarchical Clustering)). Principal component analysis enabled very well separation of the analyzed Artemisia species based on the content of total phenolics, total flavonoids, and the values obtained from antioxidant tests, but not on the individual compounds identified and quantified by HPLC. The MANOVA analyses showed that for A. scoparia and A. annua there was a significant effect of soil type on the total phenolics, total flavonoids, and antioxidant tests. In contrast, for A. vulgaris and A. absinthium, that effect was not significant. Additional MANOVA analyses showed a significant effect of soil type on phenolic and flavonoid compounds in the case of A. vulgaris, A. annua, and A. absinthium. The overall correct classification rate of all samples of four investigated Artemisia species by the discriminant analysis was 81.25 % using the training sample and 72.92 % the cross-validation results based on TP, TF, and antioxidant tests, but a much lower based on selected compounds identified and quantified by HPLC. MANOVA analyses based on particular Artemisia species show that soil is a significant factor affecting the measurable variables.
MeSH term(s)	Antioxidants/chemistry ; Artemisia ; Chemometrics ; Flavonoids/chemistry ; Phenols/chemistry ; Plant Extracts/chemistry
Chemical Substances	Antioxidants ; Flavonoids ; phenolic acid (I3P9R8317T) ; Phenols ; Plant Extracts ; 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (28752-68-3)
Language	English
Publishing date	2022-11-16
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2139001-0
ISSN	1612-1880 ; 1612-1872
ISSN (online)	1612-1880
ISSN	1612-1872
DOI	10.1002/cbdv.202200365
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Article: Thermal degradation kinetics of myrtle leaves ethanol extract (Myrtus communis L.): effect on phenolic compounds content and antioxidant activity

Snoussi, Ahmed / Bouacida, Saoussen / Mitić, Milan / Arsić, Biljana / Koubaier, Hayet Ben Haj / Chouaibi, Moncef / Janković, Sonja / Zlatanović, Ivana / Mrmošanin, Jelena / Stojanović, Gordana / Bouzouita, Nabiha

Journal of food measurement & characterization. 2022 June, v. 16, no. 3

2022

Abstract: This study was designed to examine the effect of thermal treatment at different temperatures (70, 90 and 110 °C) over time (0–120 min) on the phenolic content and antioxidant activity of Myrtus communis L. ethanol extract in acetate buffer (pH = 3). ... ...

Abstract	This study was designed to examine the effect of thermal treatment at different temperatures (70, 90 and 110 °C) over time (0–120 min) on the phenolic content and antioxidant activity of Myrtus communis L. ethanol extract in acetate buffer (pH = 3). Total phenols, flavonoids and proanthocyanidins contents and the radical scavenging activity, monitored during the treatment, were significantly influenced by the applied temperature (p < 0.05). The higher the temperature and the duration of the treatment, the greater the degradation and the loss of these compounds. Likewise, DPPH⋅ radical scavenging activity decreased, in a temperature-depending manner, during thermal treatment. Nonetheless, the results of the ABTS⋅⁺ test showed that the activity remained almost stable during the treatment. Furthermore, the kinetics of degradation of compounds analysed by HPLC-DAD, namely myricetin-3-O-galactoside, quercetin-3-O-glucoside and quercetin-3-O-rhamnoside was investigated and their energy of activation was determined. Degradation rate in case of quercetin-3-O-rhamnoside was the most sensitive to temperature (20.536 kJ/mol) followed by myricetin-3-O-galactoside (12.268 kJ/mol) and quercetin-3-O-glucoside (2.838 kJ/mol). The use of the discriminant analysis has shown that some other factors other than temperature and time of thermal treatment might also have a minor influence on the content of total phenolics, total flavonoids, proanthocyanidins and values of the antioxidant tests (DPPH⋅, ABTS⋅⁺) in the investigated extracts of M. communis L.
Keywords	Myrtus communis ; acetates ; antioxidant activity ; antioxidants ; discriminant analysis ; energy ; ethanol ; flavonoids ; heat treatment ; measurement ; pH ; proanthocyanidins ; temperature ; thermal degradation
Language	English
Dates of publication	2022-06
Size	p. 2119-2130.
Publishing place	Springer US
Document type	Article
ZDB-ID	2754612-3
ISSN	2193-4134 ; 2193-4126
ISSN (online)	2193-4134
ISSN	2193-4126
DOI	10.1007/s11694-022-01341-1
Database	NAL-Catalogue (AGRICOLA)

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Article: Wild-Growing and Conventionally or Organically Cultivated

Papagrigoriou, Theodora / Iliadi, Panagiota / Mitić, Milan N / Mrmošanin, Jelena M / Papanastasi, Katerina / Karapatzak, Eleftherios / Maloupa, Eleni / Gkourogianni, Alexia V / Badeka, Anastasia V / Krigas, Nikos / Lazari, Diamanto

Plants (Basel, Switzerland)

2023 Volume 12, Issue 8

Abstract: European elder or elderberry ( ...

Abstract	European elder or elderberry (
Language	English
Publishing date	2023-04-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants12081701
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Article ; Online: Furofuran lignans of Artemisia genus

Ickovski Jovana D. / Pavlović Jovana Lj. / Mitić Milan N. / Palić Ivan R. / Kostić Danijela A. / Petrović Goran M. / Stojanović Gordana S.

Journal of the Serbian Chemical Society, Vol 85, Iss 5, Pp 575-

Isolation, biosynthesis and biological activity

2020 Volume 600

Abstract: Since ancient times, medicinal plants and pharmacologically active products obtained from different natural sources play an important role in human health. Plants belonging to the genus Artemisia possess a great biological potential and it is a well- ... ...

Abstract	Since ancient times, medicinal plants and pharmacologically active products obtained from different natural sources play an important role in human health. Plants belonging to the genus Artemisia possess a great biological potential and it is a well-studied genus in the fields such as systematics (including molecular phylogenetics) and genome organization. Many species of the genus (e.g., A. absinthium, A. annua, A. vulgaris, A. abrotanum, A. arborescens) are widely exploited, because of their high economic value as medicines, food and ornamentals. Withal, in such a large genus, some hiatus must inevitably exist, concerning attainments and potentials that individual species possess. Most of the studies are focused on bioactivity and pharmacology of sesquiterpene lactones. Lignans are unjustly neglected, even though they as well exhibit a wide range of bioactivities. Motivated by that fact, we tried to consolidate findings on bioactive lignans accumulated through the years, with the logical perspectives on further work on isolation and identification of new bioactive lignans and the exploitation of lignans as substances of potential pharmacological interest. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 172047]
Keywords	artemisia ; lignan ; sesamin ; sesartemin ; diayangambin ; epiyangambin ; Chemistry ; QD1-999
Subject code	580
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Serbian Chemical Society
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction.

Cvetanović, Aleksandra / Zengin, Gökhan / Zeković, Zoran / Švarc-Gajić, Jaroslava / Ražić, Slavica / Damjanović, Ana / Mašković, Pavle / Mitić, Milan

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

2018 Volume 121, Page(s) 458–466

Abstract: Preparation of functional products as well as natural-based products requires non-toxic but effective extraction techniques. In this study, subcritical water extraction was used for the extraction of different aronia parts in order to explore their ... ...

Abstract	Preparation of functional products as well as natural-based products requires non-toxic but effective extraction techniques. In this study, subcritical water extraction was used for the extraction of different aronia parts in order to explore their potential. Stems, leaves and berries of Aronia melanocarpa were extracted under the following conditions: temperature 130 °C; pressure 35 bar; time 20 min. The total phenols and flavonoid contents of the produced extracts were evaluated by conventional spectrophotometric methods. Additionally, the main phenolic compounds were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). The biological potential of the extracts was evaluated by determining their antioxidant (DPPH, ABTS and lipid peroxidation assays), antimicrobial, enzyme inhibitory (cholinesterase and elastase) and cytotoxic effects (HeLa, A-549, LS-174T, MRC-5 cell lines). The results indicate that leaves and berries extracts exhibited stronger antioxidant action when compared with stems. The strongest cholinesterase and elastase inhibitory activity was also found in berries extract. Similarly, the extracts obtained from leaves and berries showed considerable cytotoxic effects against tested cell lines. A moderate antimicrobial effects was observed too. Demonstrated biological potential of all three aronia parts can trace a new road map for developing newly designed functional products.
MeSH term(s)	Antineoplastic Agents, Phytogenic/chemistry ; Antineoplastic Agents, Phytogenic/pharmacology ; Cell Line, Tumor ; Chemical Fractionation ; Fruit/chemistry ; Humans ; Photinia/chemistry ; Phytochemicals ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Plant Leaves/chemistry ; Plant Stems/chemistry ; Polyphenols/chemistry ; Polyphenols/pharmacology ; Water
Chemical Substances	Antineoplastic Agents, Phytogenic ; Phytochemicals ; Plant Extracts ; Polyphenols ; Water (059QF0KO0R)
Language	English
Publishing date	2018-09-21
Publishing country	England
Document type	Journal Article
ZDB-ID	782617-5
ISSN	1873-6351 ; 0278-6915
ISSN (online)	1873-6351
ISSN	0278-6915
DOI	10.1016/j.fct.2018.09.045
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