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  1. Article ; Online: The teleological significance of the sociological method in the study of law as a social phenomenon

    Mitrović Aleksandra Lj.

    Zbornik Radova Filozofskog Fakulteta u Prištini, Vol 49, Iss 4, Pp 71-

    2019  Volume 85

    Abstract: The aim of this scientific paper is to highlight the role and importance of the sociological method in the study of the law as a specific social phenomenon with its essence and structure-which need to be perceived as one-seeing that the law has both ... ...

    Abstract The aim of this scientific paper is to highlight the role and importance of the sociological method in the study of the law as a specific social phenomenon with its essence and structure-which need to be perceived as one-seeing that the law has both value and normative and social dimension. This stems from the relationship between rights and society, regarding the role that law has in the society and vice versa. As the nature of the object defines the method used to implement the process of research, the law as normatively shaped social phenomenon requires the use of sociological methods to explain the causes of partial or full (non) realization of legal norms in society. The conclusion is that a complex, dynamic, and variable substance and content of the law cannot be studied without the use of sociological methods, which, in addition to numerous methods of studying law, manage to investigate systematically and empirically the functional laws of the legal system as a social phenomenon.
    Keywords sociological method ; society ; law ; jurisprudence ; History of scholarship and learning. The humanities ; AZ20-999
    Subject code 340
    Language English
    Publishing date 2019-01-01T00:00:00Z
    Publisher Faculty of Philosophy, Kosovska Mitrovica
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending

    Prokopijević, Miloš / Simonović Radosavljević, Jasna / Spasojević, Dragica / Vojisavljević, Katarina / Radotić, Ksenija / Mitrović, Aleksandra Lj

    Holzforschung. 2022 July 26, v. 76, no. 7

    2022  

    Abstract: Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood (TW). Typical TW fibers, gelatinous fibers (G-fibers), are characterized by an inner gelatinous cell wall layer (G-layer). Xyloglucan endotransglycosylases (XETs) was ...

    Abstract Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood (TW). Typical TW fibers, gelatinous fibers (G-fibers), are characterized by an inner gelatinous cell wall layer (G-layer). Xyloglucan endotransglycosylases (XETs) was proposed as the essential enzyme in cell wall modifications in TW, by making xyloglucan (XG) cross-links between G- and S₂-layers in G-fibers, and thus maintaining their contact. The determination of TW presence in a sample is of great importance for the forest products industry, biofuel production, and tree physiology studies. However, TW is not easy to detect visually. The colorimetric assay for XET activity determination as an indicator of TW presence in a sample was tested on powdered stem segments of juvenile Populus x euramericana trees exposed to severe long-term static bending. In parallel, histochemical and ultrastructural characterization of stem samples of bent and control trees was performed. The tested colorimetric assay for XET activity determination could be suggested as a useful and easily applicable tool for fast screening of powdered wood samples for the presence of TW.
    Keywords Populus ; cell walls ; colorimetry ; crosslinking ; enzymes ; forests ; fuel production ; hardwood ; juveniles ; tension wood ; tree physiology ; xyloglucans
    Language English
    Dates of publication 2022-0726
    Size p. 668-673.
    Publishing place De Gruyter
    Document type Article
    ZDB-ID 1466072-6
    ISSN 1437-434X ; 0018-3830
    ISSN (online) 1437-434X
    ISSN 0018-3830
    DOI 10.1515/hf-2021-0223
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  3. Article ; Online: Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts.

    Radosavljević, Jasna Simonović / Mitrović, Aleksandra Lj / Radotić, Ksenija / Zimányi, László / Garab, Győző / Steinbach, Gábor

    International journal of molecular sciences

    2021  Volume 22, Issue 14

    Abstract: Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning microscopy and digital light microscopy imaging techniques, in general-in addition to their ... ...

    Abstract Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning microscopy and digital light microscopy imaging techniques, in general-in addition to their high sensitivity, fast data acquisition, and great versatility of 2D-4D image analyses-also opened the technical possibilities to combine microscopy imaging with spectroscopic measurements. In this review, we focus our attention on differential polarization (DP) imaging techniques and on their applications on plant cell walls and chloroplasts, and show how these techniques provided unique and quantitative information on the anisotropic molecular organization of plant cell constituents: (i) We briefly describe how laser-scanning microscopes (LSMs) and the enhanced-resolution Re-scan Confocal Microscope (RCM of Confocal.nl Ltd. Amsterdam, Netherlands) can be equipped with DP attachments-making them capable of measuring different polarization spectroscopy parameters, parallel with the 'conventional' intensity imaging. (ii) We show examples of different faces of the strong anisotropic molecular organization of chloroplast thylakoid membranes. (iii) We illustrate the use of DP imaging of cell walls from a variety of wood samples and demonstrate the use of quantitative analysis. (iv) Finally, we outline the perspectives of further technical developments of micro-spectropolarimetry imaging and its use in plant cell studies.
    MeSH term(s) Anisotropy ; Cell Wall/ultrastructure ; Chloroplasts/ultrastructure ; Microscopy, Confocal/methods ; Microscopy, Polarization/methods ; Plant Cells/ultrastructure ; Thylakoids/ultrastructure
    Language English
    Publishing date 2021-07-17
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22147661
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  4. Article: Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro

    Antić, Tijana Cvetić / Janošević, Dušica / Maksimović, Vuk M / Živić, Miroslav / Budimir, Snežana / Glamočlija, Jasmina / Mitrović, Aleksandra Lj

    Journal of plant physiology. 2020 Jan., v. 244

    2020  

    Abstract: We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation ...

    Abstract We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3–4 days post inoculation (dpi). During the necrotrophic phase, 5–7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
    Keywords Fusarium verticillioides ; antioxidant activity ; cacti and succulents ; catalase ; catechol oxidase ; enzyme activity ; fusaric acid ; histology ; hyphae ; malondialdehyde ; mesophyll ; peroxidase ; phenylalanine ammonia-lyase ; polyphenols ; spore germination ; sporulation ; superoxide dismutase
    Language English
    Dates of publication 2020-01
    Publishing place Elsevier GmbH
    Document type Article
    ZDB-ID 283647-6
    ISSN 1618-1328 ; 0176-1617
    ISSN (online) 1618-1328
    ISSN 0176-1617
    DOI 10.1016/j.jplph.2019.153086
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  5. Article: Cell wall response to UV radiation in needles of Picea omorika

    Mitrović, Aleksandra Lj / Radosavljević, Jasna Simonović / Prokopijević, Miloš / Spasojević, Dragica / Kovačević, Jovana / Prodanović, Olivera / Todorović, Bratislav / Matović, Branko / Stanković, Mira / Maksimović, Vuk / Mutavdžić, Dragosav / Skočić, Miloš / Pešić, Mirjana / Prokić, Ljiljana / Radotić, Ksenija

    Plant physiology and biochemistry. 2021 Apr., v. 161

    2021  

    Abstract: The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile ... ...

    Abstract The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day- UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of CC in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV–B), and ionic POD and covalent PO (UV–C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling.
    Keywords Picea omorika ; cell walls ; cellulose ; chlorophyll ; cinnamic acid ; crystal structure ; fluorometry ; juveniles ; lignin ; p-coumaric acid ; plant physiology ; polyphenols ; spectroscopy ; ultraviolet radiation ; xylan ; xyloglucans
    Language English
    Dates of publication 2021-04
    Size p. 176-190.
    Publishing place Elsevier Masson SAS
    Document type Article
    ZDB-ID 742978-2
    ISSN 1873-2690 ; 0981-9428
    ISSN (online) 1873-2690
    ISSN 0981-9428
    DOI 10.1016/j.plaphy.2021.02.007
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  6. Article ; Online: Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro.

    Antić, Tijana Cvetić / Janošević, Dušica / Maksimović, Vuk M / Živić, Miroslav / Budimir, Snežana / Glamočlija, Jasmina / Mitrović, Aleksandra Lj

    Journal of plant physiology

    2019  Volume 244, Page(s) 153086

    Abstract: We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation ...

    Abstract We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
    MeSH term(s) Antioxidants/metabolism ; Crassulaceae/chemistry ; Crassulaceae/microbiology ; Fusarium/physiology ; Humidity ; Hyphae/physiology ; Plant Diseases/microbiology
    Chemical Substances Antioxidants
    Language English
    Publishing date 2019-11-27
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 283647-6
    ISSN 1618-1328 ; 0176-1617
    ISSN (online) 1618-1328
    ISSN 0176-1617
    DOI 10.1016/j.jplph.2019.153086
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    In stock of ZB MED Bonn / Germany

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  7. Article ; Online: Cell wall response to UV radiation in needles of Picea omorika.

    Mitrović, Aleksandra Lj / Radosavljević, Jasna Simonović / Prokopijević, Miloš / Spasojević, Dragica / Kovačević, Jovana / Prodanović, Olivera / Todorović, Bratislav / Matović, Branko / Stanković, Mira / Maksimović, Vuk / Mutavdžić, Dragosav / Skočić, Miloš / Pešić, Mirjana / Prokić, Ljiljana / Radotić, Ksenija

    Plant physiology and biochemistry : PPB

    2021  Volume 161, Page(s) 176–190

    Abstract: The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile ... ...

    Abstract The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day- UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of CC in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV-B), and ionic POD and covalent PO (UV-C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling.
    MeSH term(s) Cell Wall ; Cellulose ; Lignin ; Picea ; Ultraviolet Rays
    Chemical Substances Cellulose (9004-34-6) ; Lignin (9005-53-2)
    Language English
    Publishing date 2021-02-10
    Publishing country France
    Document type Journal Article
    ZDB-ID 742978-2
    ISSN 1873-2690 ; 0981-9428
    ISSN (online) 1873-2690
    ISSN 0981-9428
    DOI 10.1016/j.plaphy.2021.02.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

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