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  1. Article ; Online: Rift Valley Fever.

    Kimble, J Brian / Noronha, Leela / Trujillo, Jessie D / Mitzel, Dana / Richt, Juergen A / Wilson, William C

    The Veterinary clinics of North America. Food animal practice

    2024  

    Abstract: Rift Valley fever (RVF) is a zoonotic viral disease that affects domestic and wild ruminants such as cattle, sheep, goats, camels, and buffaloes. Rift valley fever virus (RVFV), the causative agent of RVF, can also infect humans. RVFV is an arthropod- ... ...

    Abstract Rift Valley fever (RVF) is a zoonotic viral disease that affects domestic and wild ruminants such as cattle, sheep, goats, camels, and buffaloes. Rift valley fever virus (RVFV), the causative agent of RVF, can also infect humans. RVFV is an arthropod-borne virus (arbovirus) that is primarily spread through the bites of infected mosquitoes or exposure to infected blood. RVFV was first isolated and characterized in the Rift Valley of Kenya in 1931 and is endemic throughout sub-Saharan Africa, including Comoros and Madagascar, the Arabian Peninsula (Saudi Arabia and Yemen), and Mayotte.
    Language English
    Publishing date 2024-03-06
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 286086-7
    ISSN 1558-4240 ; 0749-0720
    ISSN (online) 1558-4240
    ISSN 0749-0720
    DOI 10.1016/j.cvfa.2024.01.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Erratum: AC-DC Electropenetrography as a Tool to Quantify Probing and Ingestion Behaviors of the Yellow Fever Mosquito (

    Vaughan, Lyndsi D / Jameson, Samuel B / Wesson, Dawn M / Silver, Kristopher S / Mitzel, Dana N / Dobek, Georgina L / Londoño-Renteria, Berlin

    Comparative medicine

    2024  

    Abstract: This corrects the article DOI: ... 10.30802/AALAS-CM-23-000037 ... When the above article was first published in the Vol 3 No 6 (December 2023) issue ... ...

    Abstract This corrects the article DOI: 10.30802/AALAS-CM-23-000037
    When the above article was first published in the Vol 3 No 6 (December 2023) issue of
    Language English
    Publishing date 2024-03-04
    Publishing country United States
    Document type Journal Article ; Published Erratum
    ZDB-ID 2006425-1
    ISSN 2769-819X ; 0023-6764 ; 1532-0820
    ISSN (online) 2769-819X
    ISSN 0023-6764 ; 1532-0820
    DOI 10.30802/AALAS-CM-24-000009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Artificial Feeding Systems for Vector-Borne Disease Studies.

    Olajiga, Olayinka M / Jameson, Samuel B / Carter, Brendan H / Wesson, Dawn M / Mitzel, Dana / Londono-Renteria, Berlin

    Biology

    2024  Volume 13, Issue 3

    Abstract: This review examines the advancements and methodologies of artificial feeding systems for the study of vector-borne diseases, offering a critical assessment of their development, advantages, and limitations relative to traditional live host models. It ... ...

    Abstract This review examines the advancements and methodologies of artificial feeding systems for the study of vector-borne diseases, offering a critical assessment of their development, advantages, and limitations relative to traditional live host models. It underscores the ethical considerations and practical benefits of such systems, including minimizing the use of live animals and enhancing experimental consistency. Various artificial feeding techniques are detailed, including membrane feeding, capillary feeding, and the utilization of engineered biocompatible materials, with their respective applications, efficacy, and the challenges encountered with their use also being outlined. This review also forecasts the integration of cutting-edge technologies like biomimicry, microfluidics, nanotechnology, and artificial intelligence to refine and expand the capabilities of artificial feeding systems. These innovations aim to more accurately simulate natural feeding conditions, thereby improving the reliability of studies on the transmission dynamics of vector-borne diseases. This comprehensive review serves as a foundational reference for researchers in the field, proposing a forward-looking perspective on the potential of artificial feeding systems to revolutionize vector-borne disease research.
    Language English
    Publishing date 2024-03-15
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2661517-4
    ISSN 2079-7737
    ISSN 2079-7737
    DOI 10.3390/biology13030188
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: An electropenetrography waveform library for the probing and ingestion behaviors of Culex tarsalis on human hands.

    Cooper, Anastasia M W / Jameson, Samuel B / Pickens, Victoria / Osborne, Cameron / Backus, Elaine A / Silver, Kristopher / Mitzel, Dana N

    Insect science

    2023  

    Abstract: Culex tarsalis Coquillett (Diptera: Culicidae) mosquitoes are capable of vectoring numerous pathogens affecting public and animal health. Unfortunately, the probing behaviors of mosquitoes are poorly understood because they occur in opaque tissues. ... ...

    Abstract Culex tarsalis Coquillett (Diptera: Culicidae) mosquitoes are capable of vectoring numerous pathogens affecting public and animal health. Unfortunately, the probing behaviors of mosquitoes are poorly understood because they occur in opaque tissues. Electropenetrography (EPG) has the potential to elucidate these behaviors by recording the electrical signals generated during probing. We used an AC-DC EPG with variable input resistors (Ri levels) to construct a waveform library for Cx. tarsalis feeding on human hands. Biological events associated with mosquito probing were used to characterize waveforms at four Ri levels and with two electrical current types. The optimal settings for EPG recordings of Cx. tarsalis probing on human hands was an Ri level of 10
    Language English
    Publishing date 2023-11-09
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2179775-4
    ISSN 1744-7917 ; 1672-9609
    ISSN (online) 1744-7917
    ISSN 1672-9609
    DOI 10.1111/1744-7917.13292
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Assessing the feasibility, safety, and nutritional quality of using wild-caught pest flies in animal feed.

    Van Nest, Kortnee / Swistek, Sabrina E / Olmstead, Morgan L / De La Mota-Peynado, Alina / Ewing, Robert D / Brabec, Daniel / Mitzel, Dana / Oppert, Brenda / Cohnstaedt, Lee W / Shults, Phillip

    Journal of economic entomology

    2024  

    Abstract: Studies have investigated the potential of using farmed insects in animal feeds; however, little research has been done using wild-caught insects for this purpose. Concerns about inadequate quantities collected, environmental impacts, and the spread of ... ...

    Abstract Studies have investigated the potential of using farmed insects in animal feeds; however, little research has been done using wild-caught insects for this purpose. Concerns about inadequate quantities collected, environmental impacts, and the spread of pathogens contribute to the preferred utilization of farmed insects. Nevertheless, by harvesting certain pest species from intensified agricultural operations, producers could provide their animals with affordable and sustainable protein sources while also reducing pest populations. This study explores the possibility of collecting large quantities of pest flies from livestock operations and analyzes the flies' nutritional content, potential pathogen load, and various disinfection methods. Using a newly designed mass collection-trapping device, we collected 5 kg of biomass over 13 wk, primarily house flies, from a poultry facility. While a substantial number of pests were removed from the environment, there was no reduction in the fly population. Short-read sequencing was used to compare the bacterial communities carried by flies from differing source populations, and the bacterial species present in the fly samples varied based on farm type and collection time. Drying and milling the wild-caught flies as well as applying an additional heat treatment significantly reduced the number of culturable bacteria present in or on the flies, though their pathogenicity remains unknown. Importantly, these disinfection methods did not affect the nutritional value of the processed flies. Further research is necessary to fully assess the safety and viability of integrating wild-caught insects into livestock feed; however, these data show promising results in favor of such a system.
    Language English
    Publishing date 2024-01-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 3031-4
    ISSN 1938-291X ; 0022-0493
    ISSN (online) 1938-291X
    ISSN 0022-0493
    DOI 10.1093/jee/toad239
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Establishment of a Culex tarsalis (Diptera: Culicidae) Cell Line and its Permissiveness to Arbovirus Infection.

    Schirtzinger, Erin E / Jasperson, Dane C / Swanson, Dustin A / Mitzel, Dana / Drolet, Barbara S / Richt, Juergen A / Wilson, William C

    Journal of medical entomology

    2022  Volume 60, Issue 1, Page(s) 239–244

    Abstract: A cell line was established from Culex tarsalis Coquillett embryonated eggs and designated as CxTr. The cell line is heterogeneous, composed predominantly of small, round cells, and spindle-shaped cells with a doubling time of approximately 52-60 h. The ... ...

    Abstract A cell line was established from Culex tarsalis Coquillett embryonated eggs and designated as CxTr. The cell line is heterogeneous, composed predominantly of small, round cells, and spindle-shaped cells with a doubling time of approximately 52-60 h. The identity of the cell line was verified as Cx. tarsalis by sequencing of cytochrome oxidase I and the cells were found to be free of contaminating cells, bacteria, fungi, and mycoplasma. The permissiveness of CxTr cells to arbovirus infection was investigated with vaccine and wildtype arboviruses from four viral families: Flaviviridae (Japanese encephalitis virus), Phenuiviridae (Rift Valley fever phlebovirus), Rhabdoviridae (vesicular stomatitis virus), and Togaviridae (Mayaro virus). All viruses were able to infect and replicate within CxTr cells.
    MeSH term(s) Animals ; Culicidae ; Culex ; Permissiveness ; Arbovirus Infections ; Cell Line
    Language English
    Publishing date 2022-10-19
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 410635-0
    ISSN 1938-2928 ; 0022-2585
    ISSN (online) 1938-2928
    ISSN 0022-2585
    DOI 10.1093/jme/tjac155
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Editorial: Emerging Arboviruses.

    Wilson, William C / Mitzel, Dana / Savini, Giovanni / Zientara, Stéphan / Richt, Juergen A

    Frontiers in veterinary science

    2020  Volume 7, Page(s) 593872

    Language English
    Publishing date 2020-11-06
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2020.593872
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The increasing threat of Rift Valley fever virus globalization: strategic guidance for protection and preparation.

    Gibson, Seth / Noronha, Leela E / Tubbs, Heidi / Cohnstaedt, Lee W / Wilson, William C / Mire, Chad / Mitzel, Dana / Anyamba, Assaf / Rostal, Melinda / Linthicum, Kenneth J

    Journal of medical entomology

    2023  Volume 60, Issue 6, Page(s) 1197–1213

    Abstract: Rift Valley fever virus (RVFV) (Bunyavirales: Phlebovirus) is a prominent vector-borne zoonotic disease threat to global agriculture and public health. Risks of introduction into nonendemic regions are tied to changing climate regimes and other dynamic ... ...

    Abstract Rift Valley fever virus (RVFV) (Bunyavirales: Phlebovirus) is a prominent vector-borne zoonotic disease threat to global agriculture and public health. Risks of introduction into nonendemic regions are tied to changing climate regimes and other dynamic environmental factors that are becoming more prevalent, as well as virus evolutionary factors and human/animal movement. Endemic to the African continent, RVFV has caused large epizootics at the decadal scale since the early 20th century but has spread to the Arabian Peninsula and shows increasing patterns of interepizootic transmission on the annual scale. This virus can be transmitted by mosquitoes as well as through direct contact with infected tissues and can cause sporadic to widespread morbidity and mortality in domestic ungulate livestock as well as humans. High viremias in infected livestock moved for legal and illegal trade as well as in infected mosquitoes or human travelers can spread this virus worldwide. With increasing global commerce, it is likely RVFV will be introduced to new areas with suitable hosts, mosquito vector species, and environments. However, the strong mosquito component of RVFV epidemiology combined with advancements in vaccines, diagnostics, and virus evolutionary factors create opportunities for strategies to leverage models of connectivity among potential source and emerging regions to target surveillance and mitigation activities to reduce the risk of RVFV introduction, or contain the virus should it be introduced, into new regions.
    MeSH term(s) Animals ; Humans ; Rift Valley fever virus ; Rift Valley Fever/epidemiology ; Rift Valley Fever/prevention & control ; Culicidae ; Zoonoses/prevention & control ; Phlebovirus
    Language English
    Publishing date 2023-10-20
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 410635-0
    ISSN 1938-2928 ; 0022-2585
    ISSN (online) 1938-2928
    ISSN 0022-2585
    DOI 10.1093/jme/tjad113
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: In Vitro Infection Dynamics of Japanese Encephalitis Virus in Established Porcine Cell Lines.

    Adetunji, Shakirat A / Smolensky, Dmitriy / Mitzel, Dana N / Owens, Jeana L / Chitko-McKown, Carol G / Cernicchiaro, Natalia / Noronha, Leela E

    Pathogens (Basel, Switzerland)

    2021  Volume 10, Issue 11

    Abstract: Japanese encephalitis virus (JEV) is a zoonotic mosquito-borne pathogen that regularly causes severe neurological disease in humans in Southeast Asia and the Western Pacific region. Pigs are one of the main amplifying hosts of JEV and play a central role ...

    Abstract Japanese encephalitis virus (JEV) is a zoonotic mosquito-borne pathogen that regularly causes severe neurological disease in humans in Southeast Asia and the Western Pacific region. Pigs are one of the main amplifying hosts of JEV and play a central role in the virus transmission cycle. The objective of this study was to identify in vitro cell systems to investigate early effects of JEV infection including viral replication and host cell death. Here, we demonstrate the susceptibility of several porcine cell lines to the attenuated genotype III JEV strain SA14-14-2. Monolayers of porcine nasal turbinate (PT-K75), kidney (SK-RST), testis (ST), and monocyte-derived macrophage (CΔ2+) cells were infected with SA14-14-2 for up to five days at a multiplicity of infection (MOI) of 0.1. The hamster kidney cell line BHK-21, previously shown to be susceptible to SA14-14-2, was used as a positive control. Culture supernatants and cells were collected between 0 and 120 h post infection (hpi), and monolayers were observed for cytopathic effect (CPE) using brightfield microscopy. The number of infectious virus particles was quantified by plaque assay and cell viability was determined using trypan blue staining. An indirect immunofluorescence assay was used to detect the presence of JEV NS1 antigens in cells infected at 1 MOI. All four porcine cell lines demonstrated susceptibility to SA14-14-2 and produced infectious virus by 12 hpi. Virus titers peaked at 48 hpi in CΔ2+, BHK-21, and SK-RST cells, at 72 hpi in PT-K75, and at 120 hpi in ST cells. CPE was visible in infected CΔ2+ and BHK-21 cells, but not the other three cell lines. The proportion of viable cells, as measured by trypan blue exclusion, declined after 24 hpi in BHK-21 and 48 hpi in CΔ2+ cells, but did not substantially decline in SK-RST, PT-K75 or ST cells. At 48 hpi, JEV NS1 was detected in all infected cell lines by fluorescence microscopy. These findings demonstrate several porcine cell lines which have the potential to serve as useful research tools for investigating JEV infection dynamics and host cell mechanisms in a natural amplifying host species, such as pigs, in vitro.
    Language English
    Publishing date 2021-11-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens10111468
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: In Vitro Infection Dynamics of Japanese Encephalitis Virus in Established Porcine Cell Lines

    Adetunji, Shakirat A. / Smolensky, Dmitriy / Mitzel, Dana N. / Owens, Jeana L. / Chitko-McKown, Carol G. / Cernicchiaro, Natalia / Noronha, Leela E.

    Pathogens. 2021 Nov. 12, v. 10, no. 11

    2021  

    Abstract: Japanese encephalitis virus (JEV) is a zoonotic mosquito-borne pathogen that regularly causes severe neurological disease in humans in Southeast Asia and the Western Pacific region. Pigs are one of the main amplifying hosts of JEV and play a central role ...

    Abstract Japanese encephalitis virus (JEV) is a zoonotic mosquito-borne pathogen that regularly causes severe neurological disease in humans in Southeast Asia and the Western Pacific region. Pigs are one of the main amplifying hosts of JEV and play a central role in the virus transmission cycle. The objective of this study was to identify in vitro cell systems to investigate early effects of JEV infection including viral replication and host cell death. Here, we demonstrate the susceptibility of several porcine cell lines to the attenuated genotype III JEV strain SA14-14-2. Monolayers of porcine nasal turbinate (PT-K75), kidney (SK-RST), testis (ST), and monocyte-derived macrophage (CΔ2+) cells were infected with SA14-14-2 for up to five days at a multiplicity of infection (MOI) of 0.1. The hamster kidney cell line BHK-21, previously shown to be susceptible to SA14-14-2, was used as a positive control. Culture supernatants and cells were collected between 0 and 120 h post infection (hpi), and monolayers were observed for cytopathic effect (CPE) using brightfield microscopy. The number of infectious virus particles was quantified by plaque assay and cell viability was determined using trypan blue staining. An indirect immunofluorescence assay was used to detect the presence of JEV NS1 antigens in cells infected at 1 MOI. All four porcine cell lines demonstrated susceptibility to SA14-14-2 and produced infectious virus by 12 hpi. Virus titers peaked at 48 hpi in CΔ2+, BHK-21, and SK-RST cells, at 72 hpi in PT-K75, and at 120 hpi in ST cells. CPE was visible in infected CΔ2+ and BHK-21 cells, but not the other three cell lines. The proportion of viable cells, as measured by trypan blue exclusion, declined after 24 hpi in BHK-21 and 48 hpi in CΔ2+ cells, but did not substantially decline in SK-RST, PT-K75 or ST cells. At 48 hpi, JEV NS1 was detected in all infected cell lines by fluorescence microscopy. These findings demonstrate several porcine cell lines which have the potential to serve as useful research tools for investigating JEV infection dynamics and host cell mechanisms in a natural amplifying host species, such as pigs, in vitro.
    Keywords Japanese encephalitis virus ; cell death ; cell lines ; cell viability ; cytopathogenicity ; fluorescence microscopy ; fluorescent antibody technique ; genotype ; hamsters ; hosts ; kidneys ; macrophages ; nervous system diseases ; nose ; pathogens ; swine ; testes ; virus replication ; virus transmission ; viruses ; South East Asia
    Language English
    Dates of publication 2021-1112
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens10111468
    Database NAL-Catalogue (AGRICOLA)

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