Artikel ; Online: Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro.
2021 Band 71, Seite(n) 101506
Abstract: Advanced platelet-rich fibrin (A-PRF) is an autogenous biological material obtained from peripheral blood. A-PRF extract (A-PRFe) contains a high concentration of various cytokines that are increasingly appreciated for their roles in improving stem cell ... ...
| Abstract | Advanced platelet-rich fibrin (A-PRF) is an autogenous biological material obtained from peripheral blood. A-PRF extract (A-PRFe) contains a high concentration of various cytokines that are increasingly appreciated for their roles in improving stem cell repairing function during tissue regeneration. However, the optimal A-PRFe concentration to stimulate stem cells is unknown. This study aimed to identify the optimal concentrations of A-PRFe to promote adipogenic and osteogenic differentiation of human adipose-derived stem cells (ASCs). We produced A-PRFe from A-PRF clots by centrifuging fresh peripheral blood samples and isolated and identified ASCs using surface CD markers and multilineage differentiation potential. Enzyme-linked immunosorbent assay (ELISA) showed the concentrations of several cytokines, including b-FGF, PDGF-BB, and others, increased gradually, peaked on day 7 and then decreased. Cell proliferation assays showed A-PRFe significantly stimulated ASC proliferation, and proliferation significantly increased at higher A-PRFe doses. The degree of adipogenic and osteogenic differentiation increased at higher A-PRFe concentrations in the culture medium, as determined by oil red O and alizarin red staining. Reverse transcription polymerase chain reaction (RT-PCR) showed that expression levels of genes related to adipogenic/osteogenic differentiation (PPARγ2, C/EBPα, FABP4, Adiponectin, and ALP, OPN, OCN, RUNX2), paracrine (HIF-1α, VEGF, IGF-2) and immunoregulation (HSP70, IL-8) function were higher in groups with a higher concentration of A-PRFe than in lower concentration groups. This study demonstrates that A-PRFe is ideal for use in ASC applications in regenerative medicine because it improves biological functions, including proliferation, adipogenic/osteogenic differentiation, and paracrine function in a dose-dependent manner. |
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| Mesh-Begriff(e) | Adipogenesis/drug effects ; Cell Differentiation/drug effects ; Complex Mixtures/chemistry ; Complex Mixtures/pharmacology ; Dose-Response Relationship, Drug ; Humans ; Mesenchymal Stem Cells/metabolism ; Osteogenesis/drug effects ; Platelet-Rich Fibrin/chemistry |
| Chemische Substanzen | Complex Mixtures |
| Sprache | Englisch |
| Erscheinungsdatum | 2021-02-05 |
| Erscheinungsland | Scotland |
| Dokumenttyp | Journal Article |
| ZDB-ID | 204424-9 |
| ISSN | 1532-3072 ; 0040-8166 |
| ISSN (online) | 1532-3072 |
| ISSN | 0040-8166 |
| DOI | 10.1016/j.tice.2021.101506 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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