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  1. Article ; Online: Endothelial dysfunction is dampened by early administration of fresh frozen plasma in a rodent burn shock model.

    Kelly, Edward J / Ziedins, Eriks E / Carney, Bonnie C / Moffatt, Lauren T / Shupp, Jeffrey W

    The journal of trauma and acute care surgery

    2024  

    Abstract: Background: Endothelial dysfunction has been implicated in the pathogenesis of burn shock affecting patients with large thermal injury. In response to injury, glycocalyx components like Syndecan-1 (SDC-1) are shed into circulation and have been used as ... ...

    Abstract Background: Endothelial dysfunction has been implicated in the pathogenesis of burn shock affecting patients with large thermal injury. In response to injury, glycocalyx components like Syndecan-1 (SDC-1) are shed into circulation and have been used as markers of endothelial damage. Previous work in our lab has shown plasma inclusive resuscitation (PIR) with fresh frozen plasma (FFP) ameliorates endothelial damage. However, there remains a paucity of information regarding optimal timing and dosing of PIR as well as organ specific endothelial responses to shock. We aimed to examine the impact of PIR on endothelial dysfunction using clinically-translatable timing and dosing.
    Methods: Spraque-Dawley rats were used to create thermal burns. Rats were subjected to 40% TBSA scald burns and were resuscitated with LR only, LR + albumin and LR + early 1 ml boluses of FFP at 0,2,4 and 8 hours post-injury. A late group also received LR + FFP starting at hour 10 post-injury. SDC-1 levels were quantified by ELISA and qRT-PCR analysis characterized transcription of glycocalyx components and inflammatory cytokines in the lung and spleen. Evan's blue dye (EBD) was used to quantify amount of vascular leakage.
    Results: LR + early FFP reduced EBD extravasation when compared to LR only groups while late FFP did not. When comparing LR only vs LR + early FFP, SDC-1 levels were reduced in the LR + early FFP group at hour 8, 12 and 24 (5.23 vs. 2.07, p < 0.001, 4.49 vs. 2.05, p < 0.01, 3.82 vs 2.08, p < 0.05, respectively). LR only groups had upregulation of Exostosin-1 (EXT-1) and SDC-1 in the lung compared to LR + early FFP groups (p < 0.01 and p < 0.05) as well as upregulation of cytokines IL-10 and IFN-Ƴ (p < 0.001 and p < 0.001).
    Conclusions: Early administration of LR + FFP reduces the magnitude of SDC-1 shedding and dampens the cytokine response to injury. The upregulation of glycocalyx components as a response to endothelial injury is also decreased in the lung and spleen by LR + early FFP administration.
    Language English
    Publishing date 2024-05-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2651070-4
    ISSN 2163-0763 ; 2163-0755
    ISSN (online) 2163-0763
    ISSN 2163-0755
    DOI 10.1097/TA.0000000000004373
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    Zs.A 127: Show issues Location:
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  2. Article ; Online: Treatment of hypopigmented burn hypertrophic scars with short-term topical tacrolimus does not lead to repigmentation.

    Molina, Esteban A / Travis, Taryn E / Hussein, Lou'ay / Oliver, Mary A / Keyloun, John W / Moffatt, Lauren T / Shupp, Jeffrey W / Carney, Bonnie C

    Lasers in surgery and medicine

    2024  Volume 56, Issue 2, Page(s) 175–185

    Abstract: Objectives: Dyschromia is an understudied aspect of hypertrophic scar (HTS). The use of topical tacrolimus has successfully shown repigmentation in vitiligo patients through promotion of melanogenesis and melanocyte proliferation. It was hypothesized ... ...

    Abstract Objectives: Dyschromia is an understudied aspect of hypertrophic scar (HTS). The use of topical tacrolimus has successfully shown repigmentation in vitiligo patients through promotion of melanogenesis and melanocyte proliferation. It was hypothesized that HTSs treated with topical tacrolimus would have increased repigmentation compared to controls.
    Methodology: Full-thickness burns in red Duroc pigs were either treated with excision and meshed split-thickness skin grafting or excision and no grafting, and these wounds formed hypopigmented HTSs (n = 8). Half of the scars had 0.1% tacrolimus ointment applied to the scar twice a day for 21 days, while controls had no treatment. Further, each scar was bisected with half incurring fractional ablative CO
    Results: Over time, within the grafted HTS and the NS group, there were no significant changes in MI, except for Week 3 in the -Tacro group. (+Tacro HTS= pre = 685.1 ± 42.0, w1 = 741.0 ± 54.16, w2 = 750.8 ± 59.0, w3 = 760.9 ± 49.8) (-Tacro HTS= pre = 700.4 ± 54.3, w1 = 722.3 ± 50.7, w2 = 739.6 ± 53.2, w3 = 722.7 ± 50.5). Over time, within the ungrafted HTS and the NS group, there were no significant changes in MI. (+Tacro HTS= pre = 644.9 ± 6.9, w1 = 661.6 ± 3.3, w2 = 650.3 ± 6.2, w3 = 636.3 ± 7.4) (-Tacro HTS= pre = 696.8 ± 8.0, w1 = 695.8 ± 12.3, w2 = 678.9 ± 14.0, w3 = 731.2 ± 50.3). LADD did not lead to any differential change in pigmentation compared to the non-LADD group. There was no evidence of increased melanogenesis within the tissue punch biopsies at any timepoint. There were no changes in TYR, TYRP1, or DCT gene expression after treatment.
    Conclusion: Hypopigmented HTSs treated with 0.1% tacrolimus ointment with or without LADD did not show significantly increased repigmentation. This study was limited by a shorter treatment interval than what is known to be required in vitiligo patients for repigmentation. The use of noninvasive, topical treatments to promote repigmentation are an appealing strategy to relieve morbidity associated with dyschromic burn scars and requires further investigation.
    MeSH term(s) Animals ; Humans ; Swine ; Tacrolimus/therapeutic use ; Cicatrix, Hypertrophic/drug therapy ; Cicatrix, Hypertrophic/etiology ; Vitiligo/drug therapy ; Ointments/therapeutic use ; Melanins/therapeutic use ; Hypopigmentation/drug therapy ; Hypopigmentation/etiology ; Hypertrophy/chemically induced ; Hypertrophy/complications ; Hypertrophy/drug therapy ; Burns/complications ; Formaldehyde/therapeutic use ; Treatment Outcome
    Chemical Substances Tacrolimus (WM0HAQ4WNM) ; Ointments ; Melanins ; Formaldehyde (1HG84L3525)
    Language English
    Publishing date 2024-01-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604493-1
    ISSN 1096-9101 ; 0196-8092
    ISSN (online) 1096-9101
    ISSN 0196-8092
    DOI 10.1002/lsm.23754
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  3. Article ; Online: Laser-assisted drug delivery of synthetic alpha melanocyte stimulating hormone and L-tyrosine leads to increased pigmentation area and expression of melanogenesis genes in a porcine hypertrophic scar model.

    Carney, Bonnie C / Oliver, Mary A / Kurup, Sanjana / Collins, Monica / Keyloun, John W / Moffatt, Lauren T / Shupp, Jeffrey W / Travis, Taryn E

    Lasers in surgery and medicine

    2023  Volume 55, Issue 5, Page(s) 490–502

    Abstract: Objectives: One symptom of hypertrophic scar (HTS) that can develop after burn injury is dyschromia with hyper- and hypopigmentation. There are limited treatments for these conditions. Previously, we showed there is no expression of alpha melanocyte ... ...

    Abstract Objectives: One symptom of hypertrophic scar (HTS) that can develop after burn injury is dyschromia with hyper- and hypopigmentation. There are limited treatments for these conditions. Previously, we showed there is no expression of alpha melanocyte stimulating hormone (α-MSH) in hypopigmented scars, and if these melanocytes are treated with synthetic α-MSH in vitro, they respond by repigmenting. The current study tested the same hypothesis in the in vivo environment using laser-assisted drug delivery (LADD).
    Methods: HTSs were created in red Duroc pigs. At Day 77 (pre), they were treated with CO
    Results: The areas of hyperpigmentation were variable before treatment. Therefore, data is represented as fold-change where each scar was normalized to its own pre value. Within the LADD of NDP α-MSH + l-tyrosine group, hyperpigmented areas gradually increased each week, reaching 1.3-fold over pre by Week 4. At each timepoint, area of hyperpigmentation was greater in the treated versus the control (1.04 ± 0.05 vs. 0.89 ± 0.08, 1.21 ± 0.07 vs. 0.98 ± 0.24, 1.21 ± 0.08 vs. 1.04 ± 0.11, 1.28 ± 0.11 vs. 0.94 ± 0.25; fold-change from pre-). Within the treatment group, pretreatment, levels of TYR were decreased -17.76 ± 4.52 below the level of normal skin in hypopigmented scars. After 1 treatment, potentially due to laser fractionation, the levels decreased to -43.49 ± 5.52. After 2, 3, and 4 treatments, there was ever increasing levels of TYR to almost the level of normally pigmented skin (-35.74 ± 15.72, -23.25 ± 6.80, -5.52 ± 2.22 [p < 0.01, Week 4]). This pattern was also observed for TYRP1 (pre = -12.94 ± 1.82, Week 1 = -48.85 ± 13.25 [p < 0.01], Weeks 2, 3, and 4 = -34.45 ± 14.64, -28.19 ± 4.98, -6.93 ± 3.05 [p < 0.01, Week 4]) and DCT (pre = -214.95 ± 89.42, Week 1 = -487.93 ± 126.32 [p < 0.05], Weeks 2, 3, and 4 = -219.06 ± 79.33, -72.91 ± 20.45 [p < 0.001], -76.00 ± 24.26 [p < 0.001]). Similar patterns were observed for scars treated with LADD of  l-tyrosine alone without NDP α-MSH. For each gene, in hyperpigmented scar, levels increased at Week 4 of treatment compared to Week 1 (p < 0.01).
    Conclusions: A clinically-relevant FLSR treatment method can be combined with topical delivery of synthetic α-MSH and l-tyrosine to increase the area of pigmentation and expression of melanogenesis genes in hypopigmented HTS. LADD of  l-tyrosine alone leads to increased expression of melanogenesis genes. Future studies will aim to optimize drug delivery, timing, and dosing.
    MeSH term(s) Animals ; Swine ; Cicatrix, Hypertrophic/drug therapy ; Cicatrix, Hypertrophic/genetics ; Cicatrix, Hypertrophic/pathology ; Tyrosine ; alpha-MSH/therapeutic use ; alpha-MSH/metabolism ; Pharmaceutical Preparations ; Pigmentation ; Hypopigmentation/drug therapy ; Hypopigmentation/genetics ; Hyperpigmentation/drug therapy ; Hyperpigmentation/genetics ; Lasers, Gas/therapeutic use ; Melanins/metabolism
    Chemical Substances Tyrosine (42HK56048U) ; alpha-MSH (581-05-5) ; Pharmaceutical Preparations ; Melanins
    Language English
    Publishing date 2023-04-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604493-1
    ISSN 1096-9101 ; 0196-8092
    ISSN (online) 1096-9101
    ISSN 0196-8092
    DOI 10.1002/lsm.23663
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    Zs.A 1603: Show issues Location:
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  4. Article ; Online: Rete ridges are decreased in dyschromic burn hypertrophic scar: A histological study.

    Carney, Bonnie C / Travis, Taryn E / Keyloun, John W / Moffatt, Lauren T / Johnson, Laura S / McLawhorn, Melissa M / Shupp, Jeffrey W

    Burns : journal of the International Society for Burn Injuries

    2023  Volume 50, Issue 1, Page(s) 66–74

    Abstract: Dyschromic hypertrophic scar (HTS) is a common sequelae of burn injury, however, its mechanism has not been elucidated. This work is a histological study of these scars with a focus on rete ridges. Rete ridges are important for normal skin physiology, ... ...

    Abstract Dyschromic hypertrophic scar (HTS) is a common sequelae of burn injury, however, its mechanism has not been elucidated. This work is a histological study of these scars with a focus on rete ridges. Rete ridges are important for normal skin physiology, and their absence or presence may hold mechanistic significance in post-burn HTS dyschromia. It was posited that hyper-, and hypo-pigmented areas of scars have different numbers of rete ridges. Subjects with dyschromic burn hypertrophic scar were prospectively enrolled (n = 44). Punch biopsies of hyper-, hypo-, and normally pigmented scar and skin were collected. Biopsies were paraffin embedded, sectioned, stained with H&E, and imaged. The number of rete ridges were investigated. Burn hypertrophic scars that healed without autografts were first investigated. The number of rete ridges was higher in normal skin compared to HTS that was either hypo- (p < 0.01) or hyper-pigmented (p < 0.001). This difference was similar despite scar pigmentation phenotype (p = 0.8687). Autografted hyper-pigmented scars had higher rete ridge ratio compared to non-autografted hyper-pigmented HTS (p < 0.0001). Burn hypertrophihc scars have fewer rete ridges than normal skin. This finding may explain the decreased epidermal adherence to underlying dermis associated with hypertrophic scars. Though, contrary to our hypothesis, no direct link between the extent of dyschromia and rete ridge quantity was observed, the differences in normal skin and hypertrophic scar may lead to further understanding of dyschromic scars.
    MeSH term(s) Humans ; Cicatrix, Hypertrophic/etiology ; Cicatrix, Hypertrophic/pathology ; Burns/complications ; Burns/pathology ; Skin/pathology ; Pigmentation Disorders ; Epidermis/pathology
    Language English
    Publishing date 2023-09-18
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 197308-3
    ISSN 1879-1409 ; 0305-4179
    ISSN (online) 1879-1409
    ISSN 0305-4179
    DOI 10.1016/j.burns.2023.09.005
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    Zs.A 1250: Show issues Location:
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  5. Article ; Online: Laser Treatment of Hypertrophic Scar in a Porcine Model Induces Change to Epidermal Histoarchitecture That Correlates to Improved Epidermal Barrier Function.

    Jimenez, Lesle M / Oliver, Mary A / Keyloun, John W / Moffatt, Lauren T / Travis, Taryn E / Shupp, Jeffrey W / Carney, Bonnie C

    Journal of burn care & research : official publication of the American Burn Association

    2023  Volume 44, Issue 4, Page(s) 758–768

    Abstract: Mechanisms and timing of hypertrophic scar (HTS) improvement with laser therapy are incompletely understood. Epidermal keratinocytes influence HTS through paracrine signaling, yet they are understudied compared to fibroblasts. It was hypothesized that ... ...

    Abstract Mechanisms and timing of hypertrophic scar (HTS) improvement with laser therapy are incompletely understood. Epidermal keratinocytes influence HTS through paracrine signaling, yet they are understudied compared to fibroblasts. It was hypothesized that fractional ablative CO2 laser scar revision (FLSR) would change the fibrotic histoarchitecture of the epidermis in HTS. Duroc pigs (n = 4 FLSR and n = 4 controls) were injured and allowed to form HTS. HTS and normal skin (NS) were assessed weekly by noninvasive skin probes measuring trans-epidermal water loss (TEWL) and biopsy collection. There were 4 weekly FLSR treatments. Immediate laser treatment began on day 49 postinjury (just after re-epithelialization), and early treatment began on day 77 postinjury. Punch biopsies from NS and HTS were processed and stained with H&E. Epidermal thickness and rete ridge ratios (RRR) were measured. Gene and protein expression of involucrin (IVL) and filaggrin (FIL) were examined through qRT-PCR and immunofluorescent (IF) staining. After treatment, peeling sheets of stratum corneum were apparent which were not present in the controls. TEWL was increased in HTS vs NS at day 49, indicating decreased barrier function (P = .05). In the immediate group, TEWL was significantly decreased at week 4 (P < .05). The early group was not significantly different from NS at the prelaser timepoint. After four sessions, the epidermal thickness was significantly increased in treated scars in both FLSR groups (immediate: P < .01 and early: P < .001, n = 8 scars). Early intervention significantly increased RRR (P < .05), and immediate treatment trended toward an increase. There was no increase in either epidermal thickness or RRR in the controls. In the immediate intervention group, there was increased IVL gene expression in HTS vs NS that decreased after FLSR. Eight scars had upregulated gene expression of IVL vs NS levels pretreatment (fold change [FC] > 1.5) compared to four scars at week 4. This was confirmed by IF where IVL staining decreased after FLSR. FIL gene expression trended towards a decrease in both interventions after treatment. Changes in epidermal HTS histoarchitecture and expression levels of epidermal differentiation markers were induced by FLSR. The timing of laser intervention contributed to differences in TEWL, epidermal thickness, and RRR. These data shed light on the putative mechanisms of improvement seen after FLSR treatment. Resolution of timing must be further explored to enhance efficacy. An increased understanding of the difference between the natural history of HTS improvement over time and interventional-induced changes will be critical to justifying the continued approved usage of this treatment.
    MeSH term(s) Swine ; Animals ; Cicatrix, Hypertrophic/pathology ; Burns/pathology ; Epidermis/pathology ; Skin/pathology ; Lasers ; Lasers, Gas
    Language English
    Publishing date 2023-02-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2224246-6
    ISSN 1559-0488 ; 1559-047X
    ISSN (online) 1559-0488
    ISSN 1559-047X
    DOI 10.1093/jbcr/irad010
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    Zs.A 6223: Show issues Location:
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  6. Article ; Online: Endothelial damage occurs early after inhalation injury as measured by increased syndecan-1 levels.

    Kelly, Edward J / Carney, Bonnie C / Oliver, Mary A / Keyloun, John W / Prindeze, Nicholas J / Nisar, Saira / Moffatt, Lauren T / Shupp, Jeffrey W

    Journal of burn care & research : official publication of the American Burn Association

    2023  Volume 44, Issue 4, Page(s) 769–774

    Abstract: Inhalation injury is a significant cause of morbidity and mortality in the burn patient population. However, the pathogenesis of inhalation injury and its potential involvement in burn shock is not well understood. Preclinical studies have shown ... ...

    Abstract Inhalation injury is a significant cause of morbidity and mortality in the burn patient population. However, the pathogenesis of inhalation injury and its potential involvement in burn shock is not well understood. Preclinical studies have shown endothelial injury, as measured by syndecan-1 (SDC-1) levels, to be involved in the increased vascular permeability seen in shock states. Furthermore, the lung has been identified as a site of significant SDC-1 shedding. Here we aim to characterize the contribution of endotheliopathy caused by inhalation alone in a swine model. When comparing injured animals, the fold change of circulating SDC-1 levels from preinjury was significantly higher at 2, 4, and 6 hours postinjury (P = .0045, P = .0017, and P < .001, respectively). When comparing control animals, the fold change of SDC-1 from preinjury was not significant at any timepoint. When comparing injured animals versus controls, the fold change of SDC-1 injured animals was significantly greater at 2, 4, 6, and 18 hours (P = .004, P = .03, P < .001, and P = .03, respectively). Histological sections showed higher lung injury severity compared to control uninjured lungs (0.56 vs 0.38, P < .001). This novel animal model shows significant increases in SDC-1 levels that provide evidence for the connection between smoke inhalation injury and endothelial injury. Further understanding of the mechanisms underlying inhalation injury and its contribution to shock physiology may aid in development of early, more targeted therapies.
    MeSH term(s) Humans ; Animals ; Swine ; Burns/therapy ; Lung Injury/etiology ; Lung Injury/pathology ; Syndecan-1 ; Lung/pathology ; Smoke Inhalation Injury/pathology
    Chemical Substances Syndecan-1
    Language English
    Publishing date 2023-03-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2224246-6
    ISSN 1559-0488 ; 1559-047X
    ISSN (online) 1559-0488
    ISSN 1559-047X
    DOI 10.1093/jbcr/irad018
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  7. Article ; Online: Neutrophil Extracellular Traps Are Induced by Coronavirus 2019 Disease-Positive Patient Plasma and Persist Longitudinally: A Possible Link to Endothelial Dysfunction as Measured by Syndecan-1.

    Kelly, Edward J / Oliver, Mary A / Carney, Bonnie C / Kolachana, Sindhura / Moffatt, Lauren T / Shupp, Jeffrey W

    Surgical infections

    2023  Volume 24, Issue 10, Page(s) 887–896

    Abstract: Background: ...

    Abstract Background:
    MeSH term(s) Humans ; COVID-19 ; Extracellular Traps/chemistry ; Extracellular Traps/metabolism ; Histones ; Neutrophils ; Peroxidase/analysis ; Peroxidase/metabolism ; Syndecan-1
    Chemical Substances Histones ; Peroxidase (EC 1.11.1.7) ; Syndecan-1 ; SDC1 protein, human
    Language English
    Publishing date 2023-11-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1440120-4
    ISSN 1557-8674 ; 1096-2964
    ISSN (online) 1557-8674
    ISSN 1096-2964
    DOI 10.1089/sur.2023.156
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    Zs.A 5617: Show issues Location:
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  8. Article ; Online: Examining Obesity and Its Association With Burn Injury: A Secondary Analysis of the Transfusion Requirement in Burn Care Evaluation Study.

    Kelly, Edward J / Reese, Adam D / Carney, Bonnie C / Keyloun, John W / Palmieri, Tina L / Moffatt, Lauren T / Shupp, Jeffrey W / Tejiram, Shawn

    The Journal of surgical research

    2023  Volume 290, Page(s) 221–231

    Abstract: Introduction: Literature examining the connection between obesity and burn injuries is limited. This study is a secondary analysis of a multicenter trial data set to investigate the association between burn outcomes and obesity following severe burn ... ...

    Abstract Introduction: Literature examining the connection between obesity and burn injuries is limited. This study is a secondary analysis of a multicenter trial data set to investigate the association between burn outcomes and obesity following severe burn injury.
    Materials and methods: Body mass index (BMI) was used to stratify patients as normal weight (NW; BMI 18.5-25), all obese (AO; any BMI>30), obese I (OI; BMI 30-34.9), obese II (OII; BMI 35-39.9), or obese III (OIII; BMI>40). The primary outcome examined was mortality. Secondary outcomes included hospital length of stay (LOS), number of transfusions, injury scores, infection occurrences, number of operations, ventilator days, intensive care unit LOS, and days to wound healing.
    Results: Of 335 patients included for study, 130 were obese. Median total body surface area (TBSA) was 31%, 77 patients (23%) had inhalation injury and 41 patients died. Inhalation injury was higher in OIII than NW (42.1% versus 20%, P = 0.03). Blood stream infections (BSI) were higher in OI versus NW (0.72 versus 0.33, P = 0.03). Total operations, ventilator days, days to wound healing, multiorgan dysfunction score, Acute Physiology and Chronic Health Evaluationscore, hospital LOS, and intensive care unit LOS were not significantly affected by BMI classification. Mortality was not significantly different between obesity groups. Kaplan-Meier survival curves did not significantly differ between the groups (χ
    Conclusions: No significant association between obesity and mortality was seen after burn injury. Age, TBSA, and percent full- thickness burn were independent predictors of mortality after burn injury, while BMI classification was not.
    MeSH term(s) Humans ; Burns/complications ; Burns/therapy ; Obesity/complications ; Obesity/epidemiology ; Obesity/therapy ; Blood Transfusion ; Sepsis/complications ; Organ Dysfunction Scores ; Retrospective Studies ; Length of Stay
    Language English
    Publishing date 2023-06-06
    Publishing country United States
    Document type Multicenter Study ; Journal Article
    ZDB-ID 80170-7
    ISSN 1095-8673 ; 0022-4804
    ISSN (online) 1095-8673
    ISSN 0022-4804
    DOI 10.1016/j.jss.2023.05.005
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  9. Article ; Online: Cold atmospheric plasma is bactericidal to wound-relevant pathogens and is compatible with burn wound healing.

    Oliver, Mary A / Hussein, Lou'ay K / Molina, Esteban A / Keyloun, John W / McKnight, Sydney M / Jimenez, Lesle M / Moffatt, Lauren T / Shupp, Jeffrey W / Carney, Bonnie C

    Burns : journal of the International Society for Burn Injuries

    2024  

    Abstract: Burn wound healing can be significantly delayed by infection leading to increased morbidity and hypertrophic scarring. An optimal antimicrobial agent would have the ability to kill bacteria without negatively affecting the host skin cells that are ... ...

    Abstract Burn wound healing can be significantly delayed by infection leading to increased morbidity and hypertrophic scarring. An optimal antimicrobial agent would have the ability to kill bacteria without negatively affecting the host skin cells that are required for healing. Currently available products provide antimicrobial coverage, but may also cause reductions in cell proliferation and migration. Cold atmospheric plasma is a partially ionized gas that can be produced under atmospheric pressure at room temperature. In this study a novel handheld Aceso Plasma Generator was used to produce and test Aceso Cold Plasma (ACP) in vitro and in vivo. ACP showed a potent ability to eliminate bacterial load in vitro for a number of different species. Deep partial-thickness and full-thickness wounds that were treated with ACP after burning, after excision, after autografting, and at days 5, 7, and 9 did not show any negative effects on their wound healing trajectories. On par with in vitro analysis, bioburden was decreased in treated wounds vs. control. In addition, metrics of hypertrophic scar such as dyschromia, elasticity, trans-epidermal water loss (TEWL), and epidermal and dermal thickness were the same between the two treatment groups. It is likely that ACP can be used to mitigate the risk of bacterial infection during the phase of acute burn injury while patients await surgery for definitive closure. It may also be useful in treating wounds with delayed re-epithelialization that are at risk for infection and hypertrophic scarring. A handheld cold plasma device will be useful in treating all manner of wounds and surgical sites in order to decrease bacterial burden in an efficient and highly effective manner without compromising wound healing.
    Language English
    Publishing date 2024-01-10
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 197308-3
    ISSN 1879-1409 ; 0305-4179
    ISSN (online) 1879-1409
    ISSN 0305-4179
    DOI 10.1016/j.burns.2023.12.012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: In‐depth examination of hyperproliferative healing in two breeds of Sus scrofa domesticus commonly used for research

    Funkhouser, Colton H. / Kirkpatrick, Liam D. / Smith, Robert D. / Moffatt, Lauren T. / Shupp, Jeffrey W. / Carney, Bonnie C.

    Animal models and experimental medicine. 2021 Dec., v. 4, no. 4

    2021  

    Abstract: BACKGROUND: Wound healing can result in various outcomes, including hypertrophic scar (HTS). Pigs serve as models to study wound healing as their skin shares physiologic similarity with humans. Yorkshire (Yk) and Duroc (Dc) pigs have been used to mimic ... ...

    Abstract BACKGROUND: Wound healing can result in various outcomes, including hypertrophic scar (HTS). Pigs serve as models to study wound healing as their skin shares physiologic similarity with humans. Yorkshire (Yk) and Duroc (Dc) pigs have been used to mimic normal and abnormal wound healing, respectively. The reason behind this differential healing phenotype was explored here. METHODS: Excisional wounds were made on Dc and Yk pigs and were sampled and imaged for 98 days. PCR arrays were used to determine differential gene expression. Vancouver Scar Scale (VSS) scores were given. Re‐epithelialization was analyzed. H&E, Mason's trichrome, and immunostains were used to determine cellularity, collagen content, and blood vessel density, respectively. RESULTS: Yk wounds heal to a “port wine” HTS, resembling scarring in Fitzpatrick skin types (FST) I‐III. Dc wounds heal to a dyspigmented, non‐pliable HTS, resembling scarring in FST IV–VI. Gene expression during wound healing was differentially regulated versus uninjured skin in 40/80 genes, 15 of which differed between breeds. Yk scars had a higher VSS score at all time points. Yk and Dc wounds had equivalent re‐epithelialization, collagen disorganization, and blood vessel density. CONCLUSIONS: Our findings demonstrate that Dc and Yk pigs can produce HTS. Wound creation and healing were consistent among breeds, and differences in gene expression were not sufficient to explain differences in resulting scar phenotype. Both pig breeds should be used in animal models to investigate novel therapeutics to provide insight into a treatment's effectiveness on various skin types.
    Keywords Duroc ; blood vessels ; collagen ; gene expression ; gene expression regulation ; medicine ; phenotype ; swine ; therapeutics
    Language English
    Dates of publication 2021-12
    Size p. 406-417.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ISSN 2576-2095
    DOI 10.1002/ame2.12188
    Database NAL-Catalogue (AGRICOLA)

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