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Article ; Online: Characterization, modeling, and anticancer activity of L.arginase production from marine Bacillus licheniformis OF2.

Selim, Manal S / Mounier, Marwa M / Abdelhamid, Sayeda A / Hamed, Ahmed Abdelghani / Abo Elsoud, Mostafa M / Mohamed, Sahar S

BMC biotechnology

2024 Volume 24, Issue 1, Page(s) 6

Abstract: Background: L-arginase, is a powerful anticancer that hydrolyzes L-arginine to L-ornithine and urea. This enzyme is widely distributed and expressed in organisms like plants, fungi, however very scarce from bacteria. Our study is based on isolating, ... ...

Abstract	Background: L-arginase, is a powerful anticancer that hydrolyzes L-arginine to L-ornithine and urea. This enzyme is widely distributed and expressed in organisms like plants, fungi, however very scarce from bacteria. Our study is based on isolating, purifying, and screening the marine bacteria that can produce arginase. Results: The highest arginase producing bacteria will be identified by using microbiological and molecular biology methods as Bacillus licheniformis OF2. Characterization of arginase is the objective of this study. The activity of enzyme was screened, and estimated beside partial sequencing of arginase gene was analyzed. In silico homology modeling was applied to generate the protein's 3D structure, and COACH and COFACTOR were applied to determine the protein's binding sites and biological annotations based on the I-TASSER structure prediction. The purified enzyme was undergone an in vitro anticancer test. Conclusions: L-arginase demonstrated more strong anti-cancer cells with an IC50 of 21.4 ug/ml in a dose-dependent manner. L-arginase underwent another investigation for its impact on the caspase 7 and BCL2 family of proteins (BCL2, Bax, and Bax/Bcl2). Through cell arrest in the G1/S phase, L-arginase signals the apoptotic cascade, which is supported by a flow cytometry analysis of cell cycle phases.
MeSH term(s)	Arginase/genetics ; Arginase/metabolism ; Bacillus licheniformis/genetics ; Bacillus licheniformis/metabolism ; bcl-2-Associated X Protein/genetics ; Arginine/metabolism ; Ornithine/metabolism ; Proto-Oncogene Proteins c-bcl-2
Chemical Substances	Arginase (EC 3.5.3.1) ; bcl-2-Associated X Protein ; Arginine (94ZLA3W45F) ; Ornithine (E524N2IXA3) ; Proto-Oncogene Proteins c-bcl-2
Language	English
Publishing date	2024-01-25
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2052746-9
ISSN	1472-6750 ; 1472-6750
ISSN (online)	1472-6750
ISSN	1472-6750
DOI	10.1186/s12896-024-00829-6
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Article ; Online: Evaluating the role of polysaccharide extracted from Pleurotus columbinus on cisplatin-induced oxidative renal injury.

Mohamed, Sahar S / Ibrahim, Ghada S / Ghoneim, Mona A M / Hassan, Amal I

Scientific reports

2023 Volume 13, Issue 1, Page(s) 835

Abstract: This research aimed to examine the antioxidant polysaccharide activity (PsPc-3) derived from Pleurotus columbinus (P. columbinus) on oxidative renal injury (ORI) induced by cisplatin (CP). The principal components of crude polysaccharide were assessed. ... ...

Abstract	This research aimed to examine the antioxidant polysaccharide activity (PsPc-3) derived from Pleurotus columbinus (P. columbinus) on oxidative renal injury (ORI) induced by cisplatin (CP). The principal components of crude polysaccharide were assessed. We studied the preventive impact of polysaccharide on cisplatin-induced renal damage in this study. For 21 days, we employed the CP-induced ORI rat model and divided the rats into four groups: control, CP alone, polysaccharide post CP (100 mg/kg) orally, and CP + polysaccharide (pre and post). The chemical characterization of the polysaccharide fraction PsPc-3 stated that protein was not present. PsPc-3 contained 7.2% uronic acid as assessed as 0% sulfate. PsPc-3 hydrolysate structured of Galacturonic:Glucose:Xylose and their molar proportions were 1:4:5, respectively. The average molecular weight (Mw) and molecular mass (Mn) per molecule of PsPc-3 were 5.49 × 10
MeSH term(s)	Rats ; Animals ; Cisplatin/pharmacology ; Kidney/metabolism ; Oxidative Stress ; Pleurotus/metabolism ; Antioxidants/pharmacology ; Antioxidants/metabolism ; Antineoplastic Agents/pharmacology
Chemical Substances	Cisplatin (Q20Q21Q62J) ; Antioxidants ; Antineoplastic Agents
Language	English
Publishing date	2023-01-16
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-27081-2
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Article ; Online: Characterization and in-vitro Alzheimer's properties of exopolysaccharide from Bacillus maritimus MSM1.

Selim, Manal S / Mohamed, Sahar S / Asker, Mohsen S / Ibrahim, Abeer Y / El-Newary, Samah A / El Awady, Mohamed E

Scientific reports

2023 Volume 13, Issue 1, Page(s) 11399

Abstract: Four bacterial isolates were obtained from marine sediments collected from Sahl Hashish, Hurghada Red Sea, Egypt. This study was designed to search for promising anti-Alzheimer natural polysaccharide; therefore, four isolates were screened for ... ...

Abstract	Four bacterial isolates were obtained from marine sediments collected from Sahl Hashish, Hurghada Red Sea, Egypt. This study was designed to search for promising anti-Alzheimer natural polysaccharide; therefore, four isolates were screened for exopolysaccharides (EPSs) production and acetylcholinesterase inhibition. The isolate S16 provided the highest EPS yield (7.51 g/L) and acetylcholinesterase inhibition. It was identified morphologically and genetically using 16S rRNA gene sequence analysis as Bacillus maritimus. A Physicochemical analysis of S16 exopolysaccharide (BMEPS) was estimated, which pointed to the presence of uronic acid and sulfate (24.7% and 18.3%, respectively). HPLC analysis indicated that mannuronic acid, glucuronic acid, glucose, and mannose are presented in a molar ratio of 0.8:1.0:2.8:2.3, respectively. Furthermore, FT-IR revealed an abundance of β-configurations. The GPC estimated the average molecular weight (Mw) as 4.31 × 10
MeSH term(s)	Acetylcholinesterase ; RNA, Ribosomal, 16S/genetics ; Spectroscopy, Fourier Transform Infrared ; Bacillus ; Antioxidants/chemistry ; Cholinesterase Inhibitors/chemistry
Chemical Substances	Acetylcholinesterase (EC 3.1.1.7) ; RNA, Ribosomal, 16S ; Antioxidants ; Cholinesterase Inhibitors
Language	English
Publishing date	2023-07-14
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-38172-z
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Article ; Online: Characterization of biologically active exopolysaccharide produced by Streptomyces sp. NRCG4 and its anti-Alzheimer efficacy: in-vitro targets.

Mahmoud, Manal G / Awady, Mohamed E El / Selim, Manal S / Ibrahim, Abeer Y / Ibrahim, Faten M / Mohamed, Sahar S

Journal, genetic engineering & biotechnology

2023 Volume 21, Issue 1, Page(s) 76

Abstract: Background: Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity. Marine microorganisms often produce bioactive substances with novel functions and ... ...

Abstract	Background: Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity. Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living conditions. Polysaccharides from marine microorganisms are of interest to new drug discovery. Results: The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs. Properties of exopolysaccharide (EPS) produced by an isolated Streptomyces strain were investigated for its capability to play as anti-Alzheimer. This strain was identified morphologically, physiologically, and biochemically and actually was confirmed by molecularly 16S rRNA analysis as Streptomyces sp. NRCG4 with accession number MK850242. The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC). The findings showed that NRCG4 was an acidic EPS composed of mannuronic acid, glucose, mannose, and rhamnose in a molar ratio of 1.2:1.5:2.8:1.0, respectively. NRCG4 Mw was determined to be 4.25 × 10 Conclusions: The present study highlighted those exopolysaccharides could be harnessed to improve pharmaceutical industry (anti-Alzheimer, anti-tyrosinase, anti-inflammatory, and antioxidant agents).
Language	English
Publishing date	2023-07-04
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2637420-1
ISSN	2090-5920 ; 1687-157X ; 2090-5920
ISSN (online)	2090-5920
ISSN	1687-157X ; 2090-5920
DOI	10.1186/s43141-023-00530-9
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Article ; Online: Insight into antioxidant and anti-inflammatory effects of marine bacterial natural exopolysaccharide (EPSSM) using carrageenan-induced paw edema in rats.

El Awady, Mohamed E / Mohamed, Sahar S / Abo Elsoud, Mostafa M / Mahmoud, Manal G / Anwar, Mai M / Ahmed, Mahgoub M / Eltaher, Ashraf / Magdeldin, Sameh / Attallah, Ashraf / Elhagry, Ali E / Abdelhamid, Sayeda A

Scientific reports

2024 Volume 14, Issue 1, Page(s) 5113

Abstract: Inflammation is a part of the body's intricate biological reaction to noxious stimuli and defensive reactions. So, the aim of this investigation was to study the anti-inflammatory activity of exopolysaccharide (EPSSM) using carrageenan-induced paw edema ... ...

Abstract	Inflammation is a part of the body's intricate biological reaction to noxious stimuli and defensive reactions. So, the aim of this investigation was to study the anti-inflammatory activity of exopolysaccharide (EPSSM) using carrageenan-induced paw edema in rats. A halophilic bacterial strain was isolated from marine sediments in the Red Sea in Egypt. The isolate has been visually and physiologically recognized, as well as by analyzing its 16S rRNA gene, which confirms Kocuria sp. clone Asker4. This particular isolate can be referenced using the accession number OL798051.1. EPSSM was subjected to purification and fractionation by a DEAE-cellulose column. Preliminary chemical analysis of EPSSM indicated that the monosaccharides were fructose, glucuronic acid, and xylose, with 2.0, 0.5, and 1.0, respectively. The antioxidant potential of EPSSM was investigated, and it was discovered that the level of activity increased independently of the concentrations, reaching a maximum threshold of 94.13% at 100 µg/mL of EPSSM for 120 min. Also, EPSSM at 50 mg/kg orally produced a significant anti-inflammatory effect on the carrageenan model at 2, 3, and 4 intervals. The EPSSM intervention resulted in reductions in the levels of catalase and superoxide dismutase enzymes, as well as a decrease in glutathione. Furthermore, the levels of nitric oxide, lipid peroxidation, and reactive oxygen species resulting from carrageenan-induced edema showed a significant reduction subsequent to the administration of EPSSM. Moreover, the findings indicated that the protein expression levels of cyclooxygenase-2 and interleukin-6 were reduced following treatment with EPSSM, resulting in a reduction of paw edema.
MeSH term(s)	Animals ; Rats ; Antioxidants/pharmacology ; Carrageenan ; RNA, Ribosomal, 16S ; Bacteria ; Edema/chemically induced ; Edema/drug therapy ; Inflammation ; Nitric Oxide
Chemical Substances	Antioxidants ; Carrageenan (9000-07-1) ; RNA, Ribosomal, 16S ; Nitric Oxide (31C4KY9ESH)
Language	English
Publishing date	2024-03-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-53502-5
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Article ; Online: Production, structural and biochemical characterization relevant to antitumor property of acidic exopolysaccharide produced from Bacillus sp. NRC5.

Mohamed, Sahar S / Ibrahim, Abeer Y / Asker, Mohsen S / Mahmoud, Manal G / El-Newary, Samah A

Archives of microbiology

2021 Volume 203, Issue 7, Page(s) 4337–4350

Abstract: This study targeted the production of exopolysaccharide from Bacillus sp. NRC5 grown in Egyptian seawater to use it as natural antitumor therapy. The biological activities of selected exopolysaccharide (BEPS) as an antioxidant, anti-inflammatory and ... ...

Abstract	This study targeted the production of exopolysaccharide from Bacillus sp. NRC5 grown in Egyptian seawater to use it as natural antitumor therapy. The biological activities of selected exopolysaccharide (BEPS) as an antioxidant, anti-inflammatory and anticancer have been studied. BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3). In addition, BEPS antitumor activity was tested against the Ehrlich Ascites Carcinoma (EAC) model. The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 10
MeSH term(s)	Animals ; Antineoplastic Agents/pharmacology ; Antioxidants/pharmacology ; Bacillus/chemistry ; Bacillus/metabolism ; Cell Differentiation/drug effects ; Cell Line, Tumor ; Humans ; Longevity/drug effects ; Mice ; Polysaccharides, Bacterial/biosynthesis ; Polysaccharides, Bacterial/chemistry ; Polysaccharides, Bacterial/pharmacology
Chemical Substances	Antineoplastic Agents ; Antioxidants ; Polysaccharides, Bacterial ; exopolysaccharide, Bacillus
Language	English
Publishing date	2021-06-10
Publishing country	Germany
Document type	Journal Article
ZDB-ID	124824-8
ISSN	1432-072X ; 0302-8933
ISSN (online)	1432-072X
ISSN	0302-8933
DOI	10.1007/s00203-021-02422-3
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Article: Production, structural and biochemical characterization relevant to antitumor property of acidic exopolysaccharide produced from Bacillus sp. NRC5

Mohamed, Sahar S. / Ibrahim, Abeer Y. / Asker, Mohsen S. / Mahmoud, Manal G. / El-Newary, Samah A.

Archives of microbiology. 2021 Sept., v. 203, no. 7

2021

Abstract	This study targeted the production of exopolysaccharide from Bacillus sp. NRC5 grown in Egyptian seawater to use it as natural antitumor therapy. The biological activities of selected exopolysaccharide (BEPS) as an antioxidant, anti-inflammatory and anticancer have been studied. BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3). In addition, BEPS antitumor activity was tested against the Ehrlich Ascites Carcinoma (EAC) model. The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 10⁵ g/mol. BEPS showed a promising COX-2 inhibitory effect in comparison with the reference drug celecoxib. BEPS appeared efficient anticancer property, where it killed 64.20 and 70.20% of MCF-7 and PC3 cells at 100 µg/ml, respectively (IC₅₀, 76.70, and 70.40 µg/ml, respectively). BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation. BEPS produced from Bacillus sp. NRC5 showed its antioxidant and anti-inflammatory abilities and antitumor abilities, which may all be attributed to its unique composition containing sulfated moieties and uronic acids.
Keywords	Bacillus (bacteria) ; antineoplastic activity ; antioxidants ; ascites ; breasts ; cancer therapy ; carcinoma ; drugs ; exopolysaccharides ; glucose ; humans ; longevity ; mannose ; microbiology ; models ; molecular weight ; neoplasm cells ; prostaglandin synthase ; prostatic neoplasms ; seawater
Language	English
Dates of publication	2021-09
Size	p. 4337-4350.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	124824-8
ISSN	1432-072X ; 0302-8933
ISSN (online)	1432-072X
ISSN	0302-8933
DOI	10.1007/s00203-021-02422-3
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Article ; Online: Effects of silver nanoparticles-polysaccharide on bleomycin-induced pulmonary fibrosis in rats.

Hassan, Amal I / Samir, Amer / Youssef, Hanan F / Mohamed, Sahar S / Asker, Mohsen S / Mahmoud, Manal G

The Journal of pharmacy and pharmacology

2021 Volume 73, Issue 11, Page(s) 1503–1512

Abstract: Objectives: The first goal of this study was to synthesize the silver nanoparticles Alcaligenes xylosoxidans exopolysaccharide (Ag-AXEPS). The second objective was to analyse the role of Ag-AXEPS nanoparticles (NPS) in treating bleomycin (BLM)-induced ... ...

Abstract	Objectives: The first goal of this study was to synthesize the silver nanoparticles Alcaligenes xylosoxidans exopolysaccharide (Ag-AXEPS). The second objective was to analyse the role of Ag-AXEPS nanoparticles (NPS) in treating bleomycin (BLM)-induced lung fibrosis. Methods: Intratracheal bleomycin (2.5 U/kg) was administered to prompt pulmonary fibrosis in rats, and pulmonary fibrosis was treated with Ag-AXEPS nanoparticles (100 ppm/twice a week for four weeks). Key findings: Ag-AXEPS nanoparticles significantly decreased the diversity of pulmonary inflammatory agents in rats with BLM-induced fibrosis. Reduced levels of respiratory tumor necrosis factor-alpha, monocyte chemotactic protein-1, matrix metalloproteinases (MMP-2 and MMP-9) were observed on treatment with synthesized Ag-AXEPS. Similarly, the treatment decreased IL-12, mRNA levels of BAX and plasma fibrosis markers like N-terminal procollagen III propeptide and transforming growth factor-β1. On the other hand, the treatment increased mRNA BCL2 and total antioxidant capacity. It also lowered the level of fibrosis, as was shown by a quantified pathologic study of hematoxylin-eosin-stained lung parts. The treatment, however, ensured that lung collagen was restored, as assessed by Masson's trichrome stain, and that overall survival was increased and enhanced. Conclusions: Our work showed that nanoparticles could be obtained at 37°C and may be a possible pulmonary fibrosis therapeutic agent.
MeSH term(s)	Alcaligenes ; Animals ; Antibiotics, Antineoplastic/adverse effects ; Antioxidants/pharmacology ; Antioxidants/therapeutic use ; Apoptosis ; Bleomycin/adverse effects ; Collagen/metabolism ; Fibrosis ; Inflammation/chemically induced ; Inflammation/metabolism ; Inflammation/prevention & control ; Lung/drug effects ; Lung/pathology ; Male ; Matrix Metalloproteinases/metabolism ; Metal Nanoparticles/therapeutic use ; Nanoconjugates/therapeutic use ; Nanoparticles/therapeutic use ; Pneumonia/chemically induced ; Pneumonia/metabolism ; Pneumonia/prevention & control ; Polysaccharides/pharmacology ; Polysaccharides/therapeutic use ; Polysaccharides, Bacterial/pharmacology ; Polysaccharides, Bacterial/therapeutic use ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Pulmonary Fibrosis/chemically induced ; Pulmonary Fibrosis/drug therapy ; Pulmonary Fibrosis/metabolism ; Pulmonary Fibrosis/pathology ; Rats, Sprague-Dawley ; Silver/pharmacology ; Silver/therapeutic use ; Transforming Growth Factor beta1/metabolism ; bcl-2-Associated X Protein/metabolism ; Rats
Chemical Substances	Antibiotics, Antineoplastic ; Antioxidants ; Nanoconjugates ; Polysaccharides ; Polysaccharides, Bacterial ; Proto-Oncogene Proteins c-bcl-2 ; Tgfb1 protein, rat ; Transforming Growth Factor beta1 ; bcl-2-Associated X Protein ; Bleomycin (11056-06-7) ; Silver (3M4G523W1G) ; Collagen (9007-34-5) ; Matrix Metalloproteinases (EC 3.4.24.-)
Language	English
Publishing date	2021-09-13
Publishing country	England
Document type	Journal Article
ZDB-ID	3107-0
ISSN	2042-7158 ; 0022-3573 ; 0373-1022
ISSN (online)	2042-7158
ISSN	0022-3573 ; 0373-1022
DOI	10.1093/jpp/rgab037
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Article: Study of the chemical structure of exopolysaccharide produced from streptomycete and its effect as an attenuate for antineoplastic drug 5-fluorouracil that induced gastrointestinal toxicity in rats

Mahmoud, Manal G / Selim, Manal S / Mohamed, Sahar S / Hassan, Amal I / Abdal-Aziz, Samia A

Animal biotechnology. 2020 Sept. 11, v. 31, no. 5

2020

Abstract: Chemotherapeutic medications, including 5 – fluorouracil (5FU), are the same old technique to most cancers and are associated with numerous peripheral toxicities. We investigated exopolysaccharide (EPSST) produced from the isolated streptomycete of the ... ...

Abstract	Chemotherapeutic medications, including 5 – fluorouracil (5FU), are the same old technique to most cancers and are associated with numerous peripheral toxicities. We investigated exopolysaccharide (EPSST) produced from the isolated streptomycete of the Mediterranean Sea for the capability to lower the severity of mucositis in vivo. The streptomycete was isolated from Mediterranean Sea sediment from the beaches of Port Said Governorates, Egypt and identified morphologically, physiologically, and biochemically and confirmed by molecularly 16S rDNA analysis. The EPSST was extracted from the supernatant of streptomycete by using 4 volumes chilled ethanol and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), and high-performance liquid chromatography (HPLC). In addition, antioxidant activity was measured through the usage of 2, 2-diphenyl-1-picrylhydrazyl (DPPH). Thirty-two male rats (180–200 g) were randomly divided into a control group (normal saline), intraperitoneal injection of 5-fluorouracil (5-FU, 150 mg/kg), normal rats were treated with EPSST and 5-FU + EPSST group. These groups were continued up to the day of sacrifice (28 days post treatments). The isolated strain became recognized based totally on 16S rDNA sequence as Streptomyce sp. with accession number SAMN08349905. The chemical evaluations of EPSST were galacturonic, glucose, galactose, mannose, and arabinose with a relative ratio of 2.1: 1: 5.37: 1.62: 1.29 individually, with an average molecular weight (Mw) 9.687 × 10³ g/mol. Also, the EPSST contained uronic acid (16%) and sulfate (12.149%) and no protein was detected. EPSST inhibited the DPPH radical activity. The findings of this study propose that EPSST inhibits 5-FU-induced mucositis through adjustment of oxidative stress, apoptosis, inflammatory factors, activation of antioxidant enzymes. The clinical administration of EPSST may recover the chemotherapy-induced intestinal dysfunction, consequently increasing the clinical efficiency of chemotherapy. In addition, the administration of EPSST reduced 5-FU-induced histopathological incongruities such as neutrophil infiltration, loss of cellular integrity, and villus and crypt distortion. The clinical administration of EPSST may recover the chemotherapy-induced intestinal dysfunction, consequently increasing the clinical efficiency of chemotherapy.
Keywords	antioxidant activity ; apoptosis ; arabinose ; biotechnology ; drug therapy ; ethanol ; exopolysaccharides ; fluorouracil ; galactose ; glucose ; high performance liquid chromatography ; histopathology ; intestines ; intraperitoneal injection ; males ; mannose ; molecular weight ; neutrophils ; nucleotide sequences ; oxidative stress ; sediments ; sulfates ; toxicity ; uronic acids ; villi ; Egypt ; Mediterranean Sea
Language	English
Dates of publication	2020-0911
Size	p. 397-412.
Publishing place	Taylor & Francis
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2043243-4
ISSN	1532-2378 ; 1049-5398
ISSN (online)	1532-2378
ISSN	1049-5398
DOI	10.1080/10495398.2019.1610416
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Article ; Online: Study of the chemical structure of exopolysaccharide produced from streptomycete and its effect as an attenuate for antineoplastic drug 5-fluorouracil that induced gastrointestinal toxicity in rats.

Mahmoud, Manal G / Selim, Manal S / Mohamed, Sahar S / Hassan, Amal I / Abdal-Aziz, Samia A

Animal biotechnology

2019 Volume 31, Issue 5, Page(s) 397–412

Abstract: Chemotherapeutic medications, including 5 - fluorouracil (5FU), are the same old technique to most cancers and are associated with numerous peripheral toxicities. We investigated exopolysaccharide (EPSST) produced from the isolated streptomycete of the ... ...

Abstract	Chemotherapeutic medications, including 5 - fluorouracil (5FU), are the same old technique to most cancers and are associated with numerous peripheral toxicities. We investigated exopolysaccharide (EPSST) produced from the isolated streptomycete of the Mediterranean Sea for the capability to lower the severity of mucositis
MeSH term(s)	Animals ; Antineoplastic Agents/adverse effects ; Apoptosis ; Fluorouracil/adverse effects ; Intestinal Mucosa/drug effects ; Male ; Mucositis/chemically induced ; Oxidative Stress ; Polysaccharides, Bacterial/chemistry ; Polysaccharides, Bacterial/pharmacology ; Rats ; Rats, Sprague-Dawley ; Streptomyces/chemistry
Chemical Substances	Antineoplastic Agents ; Polysaccharides, Bacterial ; Fluorouracil (U3P01618RT)
Language	English
Publishing date	2019-05-12
Publishing country	England
Document type	Journal Article
ZDB-ID	2043243-4
ISSN	1532-2378 ; 1049-5398
ISSN (online)	1532-2378
ISSN	1049-5398
DOI	10.1080/10495398.2019.1610416
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