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  1. Article ; Online: Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy

    Mohamed Ghoneim / Catherine A. Musselman

    STAR Protocols, Vol 4, Iss 2, Pp 102229- (2023)

    2023  

    Abstract: Summary: Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in understanding the interaction of chromatin-associated factors with nucleosomes, the basic building unit of chromatin. Here, we present a protocol for preparing ... ...

    Abstract Summary: Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in understanding the interaction of chromatin-associated factors with nucleosomes, the basic building unit of chromatin. Here, we present a protocol for preparing doubly labeled fluorescent nucleosomes for SMFM. We describe steps for over-expression in E. coli and purification of recombinant human core histones. We then detail fluorescent labeling of histones and nucleosomal double-stranded DNA followed by octamer refolding and nucleosome reconstitution. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
    Keywords Biophysics ; Single-molecule Assays ; Microscopy ; Molecular Biology ; Molecular/Chemical Probes ; Protein Biochemistry ; Science (General) ; Q1-390
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Transient Thermal Performance of Power Cable Ascertained Using Finite Element Analysis

    Sherif Salama Mohamed Ghoneim / Mahrous Ahmed / Nehmdoh Abdelmotaleb Sabiha

    Processes, Vol 9, Iss 3, p

    2021  Volume 438

    Abstract: This paper presents the computation of the cable ampacity and the temperature distribution through long duration based on the equivalent thermal circuit based on IEC 60287 standard and the Finite element method using COMSOL (Multiphysics environment, ... ...

    Abstract This paper presents the computation of the cable ampacity and the temperature distribution through long duration based on the equivalent thermal circuit based on IEC 60287 standard and the Finite element method using COMSOL (Multiphysics environment, version 5.5). This study investigated the cable ampacity and the temperature rise of the cable core and sheath at steady state and emergency conditions. The cable ampacity was investigated at different conditions such as the variation of cable depth, soil properties, and soil temperature. The results confirmed the adaptation between the thermal circuit results and the COMSOL results as well as the effectiveness of using the numerical method to compute the cable ampacity. Using the COMSOL-based thermal properties evaluations, the transient performance of the cable is ascertained. The transient study is performed for different cable loading currents and dry zone sizes.
    Keywords ampacity ; transient thermal performance of cable ; finite element method ; COMSOL ; Chemical technology ; TP1-1185 ; Chemistry ; QD1-999
    Subject code 621
    Language English
    Publishing date 2021-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Evaluation of Endothelial Dysfunction and Autophagy in Fibromyalgia-Related Vascular and Cerebral Cortical Changes and the Ameliorative Effect of Fisetin

    Fatma Mohamed Ghoneim / Salwa Mohamed Abo-Elkhair / Ayman Zaky Elsamanoudy / Dalia A. Shabaan

    Cells, Vol 11, Iss 48, p

    2022  Volume 48

    Abstract: Fibromyalgia (FM) is a common chronic pain syndrome that affects 1% to 5% of the population. We aimed to investigate the role of endothelial dysfunction and autophagy in fibromyalgia-related vascular and cerebral cortical changes in a reserpine-induced ... ...

    Abstract Fibromyalgia (FM) is a common chronic pain syndrome that affects 1% to 5% of the population. We aimed to investigate the role of endothelial dysfunction and autophagy in fibromyalgia-related vascular and cerebral cortical changes in a reserpine-induced rat model of fibromyalgia at the histological and molecular levels and to study the ameliorative effect of fisetin. Forty adult female albino rats were divided into four groups (10 each): two control groups, the reserpine-induced fibromyalgia group, and the fisetin-treated group. The carotid arteries and brains of the animals were dissected. Frozen tissue samples were used for total RNA extraction and qPCR analysis of eNOS, caspase-3, Bcl-2, LC-3, BECN-1, CHOP, and TNF-α expression. Histological, immunohistochemical (eNOS), and ultrastructure studies were conducted. The carotid arteries revealed excessive autophagy and endothelial, vascular, and apoptotic changes. The cerebral cortex showed similar findings apart from endoplasmic reticulum stress. Additionally, there was decreased gene expression of eNOS and Bcl-2 and increased expression of caspase-3, LC-3, BECN-1, CHOP, and TNF-α. In the fisetin-treated rats, improvements in the histological and molecular results were detected. In conclusion, oxidative stress, enhanced apoptosis, and excessive autophagy are fundamental pathophysiologic mechanisms of reserpine-induced fibromyalgia. Moreover, fisetin has an ameliorative effect against fibromyalgia.
    Keywords fibromyalgia ; reserpine ; fisetin ; endothelial dysfunction ; autophagy ; Biology (General) ; QH301-705.5
    Subject code 630
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer

    Marcus D. Wilson / Ludovic Renault / Daniel P. Maskell / Mohamed Ghoneim / Valerie E. Pye / Andrea Nans / David S. Rueda / Peter Cherepanov / Alessandro Costa

    Nature Communications, Vol 10, Iss 1, Pp 1-

    2019  Volume 10

    Abstract: Retroviral integrases catalyze the insertion of viral DNA into the host cell DNA and can use nucleosomes as substrates for integration. Here the authors present the 3.9 Å cryo-EM structure of prototype foamy virus integrase after strand transfer into ... ...

    Abstract Retroviral integrases catalyze the insertion of viral DNA into the host cell DNA and can use nucleosomes as substrates for integration. Here the authors present the 3.9 Å cryo-EM structure of prototype foamy virus integrase after strand transfer into nucleosomal DNA, which together with single-molecule FRET measurements provides evidence for a DNA looping and sliding mechanism of integrases.
    Keywords Science ; Q
    Language English
    Publishing date 2019-09-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer

    Marcus D. Wilson / Ludovic Renault / Daniel P. Maskell / Mohamed Ghoneim / Valerie E. Pye / Andrea Nans / David S. Rueda / Peter Cherepanov / Alessandro Costa

    Nature Communications, Vol 10, Iss 1, Pp 1-

    2019  Volume 10

    Abstract: Retroviral integrases catalyze the insertion of viral DNA into the host cell DNA and can use nucleosomes as substrates for integration. Here the authors present the 3.9 Å cryo-EM structure of prototype foamy virus integrase after strand transfer into ... ...

    Abstract Retroviral integrases catalyze the insertion of viral DNA into the host cell DNA and can use nucleosomes as substrates for integration. Here the authors present the 3.9 Å cryo-EM structure of prototype foamy virus integrase after strand transfer into nucleosomal DNA, which together with single-molecule FRET measurements provides evidence for a DNA looping and sliding mechanism of integrases.
    Keywords Science ; Q
    Language English
    Publishing date 2019-09-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Spinal Compression by Brown Tumor in Two Patients With Chronic Kidney Allograft Failure on Maintenance Hemodialysis

    Osama Gheith / Hesham Ammar / Ahmed Akl / Ahmed Hamdy / Mohamed El-saeed / Tamer El-salamouny / Mohamed Adel Bakr / Mohamed Ghoneim

    Iranian Journal of Kidney Diseases, Vol 4, Iss 3, Pp 256-

    2010  Volume 259

    Abstract: Brown tumors with non-neoplastic process are noticed in patients with end-stage renal disease suffering from a severe form of secondary hyperparathyroidism. Herein, we report a patient with chronic kidney allograft failure returned back to hemodialysis ... ...

    Abstract Brown tumors with non-neoplastic process are noticed in patients with end-stage renal disease suffering from a severe form of secondary hyperparathyroidism. Herein, we report a patient with chronic kidney allograft failure returned back to hemodialysis who experienced manifestations of cauda equina compression secondary to a lumbar brown tumor. Also, we had another patient on hemodialysis with a demineralized lesion affecting the cervical vertebrae. Although brown tumor is a rare complication, these two cases highlighted the importance of neurological symptoms in uremic patients. Spinal decompression surgery, in order to alleviate pressure on neurological structures, together with subtotal parathyroidectomy, were highly indicated.
    Keywords Diseases of the genitourinary system. Urology ; RC870-923 ; Specialties of internal medicine ; RC581-951 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Urology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Language English
    Publishing date 2010-07-01T00:00:00Z
    Publisher Iranian Society of Nephrology
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Steroid and Azathioprine Versus Steroid, Cyclosporine, and Azathioprine Therapies in Primary Haplo-Identical Living Donor Kidney Transplantation

    Osama A Gheith / Mohamed A Bakr / Mohamed A Fouda / Ahmed A Shokeir / Ahmed Bayoumy / Mohamed Sobh / Mohamed Ghoneim

    Iranian Journal of Kidney Diseases, Vol 2, Iss 1, Pp 34-

    Twenty-Year Experience

    2009  Volume 39

    Abstract: Introduction. Achievements in short-term graft survival since the introduction of cyclosporine has not been matched by improvement in long-term graft function, and chronic allograft nephropathy remains the second commonest cause of graft attrition over ... ...

    Abstract Introduction. Achievements in short-term graft survival since the introduction of cyclosporine has not been matched by improvement in long-term graft function, and chronic allograft nephropathy remains the second commonest cause of graft attrition over time. We aimed to evaluate the long-term results of conventional immunosuppression by steroid and azathioprine in comparison with cyclosporine-based triple therapy in living donor kidney transplants. Materials and Methods. We evaluated the long-term follow-up data of 369 living related kidney transplant recipients that were on prednisolone-azathioprine immunosuppressive therapy (group 1) or triple therapy by prednisolone, cyclosporine, and azathioprine (group 2). All recipients were followed-up for more than 10 years (mean, 240 ± 12 months). Comparative analyses included patient and graft survival rates, condition at last follow-up, graft rejection, and graft function. Results. There were 130 patients in group 1 and 239 in group 2. The overall frequency of acute rejection episodes was not significantly different between the two groups. However, the proportion of patients with chronic allograft nephropathy was significantly higher in group 2 (21% versus 35%, P = .001). Graft survival rates were 85.3% versus 92.4% at 1 year, 69.9% versus 71.9% at 5 years, and 52.5% versus 50.8% at 10 years in groups 1 and 2, respectively (P = .03). The two groups were comparable regarding posttransplant malignancies, diabetes mellitus, serious bacterial infections, and hepatic diseases. However, hypertensive patients were significantly more frequent in group 2. Conclusions. Chronic allograft nephropathy was significantly higher in patients receiving cyclosporine, possibly due to the risk of drug-induced nephrotoxicity, glomerular disease recurrence, and hypertension. Nowadays, it is possible to achieve excellent calcineurin inhibitors-free regimen using newer maintenance immunosuppressive agents.
    Keywords Diseases of the genitourinary system. Urology ; RC870-923 ; Specialties of internal medicine ; RC581-951 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Urology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Subject code 610
    Language English
    Publishing date 2009-04-01T00:00:00Z
    Publisher Iranian Society of Nephrology
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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