LIVIVO - Search results -

Search results

Result 1 - 9 of total 9

Search options

Article ; Online: Interference of B lymphocyte tolerance by prolactin in rheumatic autoimmune diseases

Mohammad Javad Mousavi / Ardalan Alizadeh / Somayeh Ghotloo

Heliyon, Vol 9, Iss 6, Pp e16977- (2023)

2023

Abstract: Systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and systemic sclerosis (SSc) are the most common rheumatic autoimmune diseases/disorders (RADs) that affect autologous connective tissues as a result of the breakdown of the self-tolerance ... ...

Abstract	Systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and systemic sclerosis (SSc) are the most common rheumatic autoimmune diseases/disorders (RADs) that affect autologous connective tissues as a result of the breakdown of the self-tolerance mechanisms of the immune system. Prolactin, a glycoprotein hormone, has been known for its crucial role in the pathogenesis of these rheumatic autoimmune diseases. In addition to regulating lymphocyte proliferation and antibody synthesis, prolactin is also responsible for regulating cytokine production. Moreover, it contributes to the breakdown of central and peripheral tolerance mechanisms of B lymphocytes. Given the crucial role of prolactin in the pathogenesis of the mentioned RADs, prolactin may contribute to their pathogenesis by the breakdown of tolerance. In the present study, the key role of prolactin to the breakdown of B lymphocyte tolerance and its possible implication for the pathogenesis of these diseases is discussed. Current literature supports prolactin's role in the breakdown of B lymphocyte central and peripheral tolerance mechanisms, such apoptosis, receptor editing, and also anergy. Therefore, prolactin may contribute to the pathogenesis of RADs by the breakdown of B lymphocyte tolerance. However, more investigations, particularly in RA and SSc animal models, are required to precisely address the pathologic role of prolactin.
Keywords	Autoimmune disease ; B lymphocyte ; B lymphocyte tolerance ; Prolactin ; Rheumatic autoimmune disease ; Tolerance ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
Subject code	610
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Treatment of traumatic brain injury from the viewpoint of Avicenna (Ibn Sina)

Reza Vaghebin / Mohsen Khalili / Sedigheh Amiresmaili / Hasan Namdar / Mohammad Javad Mousavi

Interdisciplinary Neurosurgery, Vol 28, Iss , Pp 101498- (2022)

A historical review

2022

Abstract: Since the edifice of conventional medicine is built on the pillars of past achievements, review or re-investigate the old medical manuscripts may lead to significant achievements. Avicenna was a famous Persian medieval physician and one of the pioneers ... ...

Abstract	Since the edifice of conventional medicine is built on the pillars of past achievements, review or re-investigate the old medical manuscripts may lead to significant achievements. Avicenna was a famous Persian medieval physician and one of the pioneers of neuroscience. The aim of this study is to evaluate Avicenna's approach to traumatic brain injury (TBI), based on his main book on medicine, “Canon of Medicine”. Avicenna divided the TBI treatments into three categories: topical therapies, oral therapies, such as diets and medications, and manipulation therapies including phlebotomy, enema, foot bath, cupping, and massage. Although some of these recommendations are supported by current knowledge, many of them are still unknown and need scientific investigation to expand our knowledge of TBI treatments in the medieval period.
Keywords	Traumatic brain injury ; Complementary and Alternative Medicine (CAM) ; Persian medicine ; Avicenna ; Surgery ; RD1-811 ; Neurology. Diseases of the nervous system ; RC346-429
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Anticoagulative Effect of Two Species of Brown Algae; Sargassum angustifolium and Cystoseira indica

Narges Obeidi / Mohammad Javad Mousavi / Arghavan Hosseinpouri / Hamideh Malekhayati / Elham Ehsandoost

Research in Molecular Medicine, Vol 8, Iss 3, Pp 115-

2020 Volume 122

Abstract: Background: Fucoidans are a group of sulfated fucose-rich polysaccharides that are isolated from brown marine algae and echinoderms, and recently have been found in seagrasses. Fucoidans, as well as their derivatives, have several beneficial biological ... ...

Abstract	Background: Fucoidans are a group of sulfated fucose-rich polysaccharides that are isolated from brown marine algae and echinoderms, and recently have been found in seagrasses. Fucoidans, as well as their derivatives, have several beneficial biological effects and therapeutic potentials. In the present study, we aimed to evaluate the anticoagulative effects of two species of brown algae, namely Sargassum angustifolium (S. angustifolium) and Cystoseira indica (C. indica). Methods: Fucoidan C and fucoidan S were extracted by an ethanol/water solvent system from S. angustifolium and C. indicia, respectively. The anticoagulative effects of fucoidan C and fucoidan S were tested on 10 normal serum samples by evaluating the rate of thrombin time (PT) and prothrombin time (PTT). Results: Both fucoidan C and fucoidan S significantly increased PTT. However, no significant difference was observed in PT. Fucoidan C had a greater effect on PTT prolongation compared with fucoidan S. Conclusion: Both fucoidans extracted from S. angustifolium and C. indicia can be used as anticoagulants in biotechnology and human disorders.
Keywords	fucoidan ; sargassum angustifolium ; cystoseira indica ; anticoagulation ; Medicine (General) ; R5-920
Language	English
Publishing date	2020-08-01T00:00:00Z
Publisher	Mazandaran University of Medical Sciences and Health Services
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: A Convenient Method for Solubilization and Refolding Recombinant Proteins

Fahimeh Maleki / Kazem Mashayekhi / Seyedeh Elham Badiee Kheirabadi / Mohammad Javad Mousavi / Mojtaba Sankian

Research in Molecular Medicine, Vol 8, Iss 1, Pp 9-

An Experience from Recombinant Mouse TGF-β1

2020 Volume 16

Abstract: Background: The production of recombinant proteins in Escherichia coli is one of the most valuable achievements in biotechnology, with many therapeutic and diagnostic applications; however, the aggregation and misfolding of proteins that result in the ... ...

Abstract	Background: The production of recombinant proteins in Escherichia coli is one of the most valuable achievements in biotechnology, with many therapeutic and diagnostic applications; however, the aggregation and misfolding of proteins that result in the formation of insoluble inclusion bodies is a disruptive factor in this process. Various solubilization and refolding methods can be used to improve recombinant protein conformation. In this study, we applied a dilution method with a refolding buffer to produce a native form of soluble immature mouse TGF-β1. Materials and Methods: The TGF-β1 cDNA which encodes the protein without the signal peptide, was cloned into the pET21-b (+) vector. The target protein was expressed by the transformation of E. coli BL21 cells with the plasmid. The resulting inclusion bodies were solubilized and then diluted in the refolding buffer to make a protein with native structure. Results: Following PCR of the recombinant plasmid with T7 primers, electrophoresis and sequencing of the amplified product indicated 100% identity of the target sequence with the murine TGF-β1 gene. Finally, the protein solubility and immuno-reactivity were confirmed a 44 kDa protein which conducted with the anti-TGF-β1-specific polyclonal antibody on a western blot. Conclusion: Our dilution method and refolding buffer effectively converted aggregated immature mouse TGF-β1 to a soluble and immuno-reactive form.
Keywords	inclusion bodies ; mouse tgf-β1 ; protein expression ; refolding protein ; Medicine (General) ; R5-920
Subject code	500
Language	English
Publishing date	2020-02-01T00:00:00Z
Publisher	Mazandaran University of Medical Sciences and Health Services
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Use of Complementary Medicine in SARS-CoV-2 and MERS-CoV

Maryam Rahmannia / Marzieh Gholampoor / Narges Ketabchi / Leila Miri / Shabboo Ansari / Maryam Fallah / Zainab MohieAldeen / Zaynab Nassereldine / Elnaz Ghafoori / Zaynab Ghashamy / Hossein Goudarzi / Seyyed Mohammad Javad Mousavi / Mohammad Javad Nasiri

Novelty in Biomedicine, Vol 10, Iss 2, Pp 128-

a Narrative Review

2022 Volume 139

Abstract: Severe acute respiration syndrome coronavirus 2 (SARS-CoV-2) is characterized by severe cytokine storm syndrome following inflammation. SARS-CoV-2 is the 7th coronavirus that causes infection in human bodies; SARS-CoV, MERS-CoV, and SARS-CoV-2 can ... ...

Abstract	Severe acute respiration syndrome coronavirus 2 (SARS-CoV-2) is characterized by severe cytokine storm syndrome following inflammation. SARS-CoV-2 is the 7th coronavirus that causes infection in human bodies; SARS-CoV, MERS-CoV, and SARS-CoV-2 can purpose severe diseases. SARS-CoV-2 at once interacts with angiotensin-converting enzyme 2 (ACE-2) receptors inside the body and causes respiratory problems. Interestingly, complementary medicines and herbal drugs affect the expression of IgE and IgG antibodies and improve the immune system; for that reason, complementary medicine could be beneficial for infectious diseases like SARS-COV-2. In this review, we assessed some related articles to evaluate the effect of complementary medicine on SARS-COV2 and MERS-COV.
Keywords	covid-19 ; coronavirus ; sars-cov-2 ; mers-cov ; Medicine (General) ; R5-920
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Shahid Beheshti University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Evaluation of TAK-242 (Resatorvid) Effects on Inflammatory Status of Fibroblast-like Synoviocytes in Rheumatoid Arthritis and Trauma Patients

Jafar Karami / Elham Farhadi / Ali-Akbar Delbandi / Mehdi Shekarabi / Mohammad Naghi Tahmasebi / Arash Sharafat Vaziri / Maryam Akhtari / Mohammad Javad Mousavi / Ahmadreza Jamshidi / Mahdi Mahmoudi

Iranian Journal of Allergy, Asthma and Immunology, Vol 20, Iss

2021 Volume 4

Abstract: Fibroblast-like synoviocytes (FLSs) produce lots of inflammatory molecules that trigger immune responses and intensification the inflammation and thereby play important roles in Rheumatoid Arthritis )RA( pathogenesis. Due to the important roles of toll- ... ...

Abstract	Fibroblast-like synoviocytes (FLSs) produce lots of inflammatory molecules that trigger immune responses and intensification the inflammation and thereby play important roles in Rheumatoid Arthritis )RA( pathogenesis. Due to the important roles of toll-like receptor 4 (TLR4) in cytokine production and inflammation, we aimed to evaluate the effects of TAK-242 (Resatorvid) on interleukin (IL)1-β, IL-6, TNF-α, and TLR4 expression and two important proteins of nuclear factor-κB (NF-κB) signaling pathway (Ikβα and pIkβα) in RA and trauma FLSs. FLSs were isolated from synovial tissues of trauma (n=10) and RA (n=10) patients and cultured in Dulbecco's Modified Eagle Medium (DMEM). 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was performed to evaluate the cytotoxicity effects of TAK-242 on the RA FLSs. Real-time PCR was performed to measure the expression level of IL1-β, IL-6, TNF-α, and TLR4 genes in Lipopolysaccharide (LPS) and TAK-242 treated FLSs. Furthermore, the treated FLSs were evaluated for protein levels of Ikβα and pIkβα by western blot. The baseline expression of IL1-β, IL-6, TNF-α, and TLR4 showed no significant differences between healthy and RA FLSs. LPS stimulated FLSs significantly increased mRNA levels of IL-1β, IL-6, TNF-α, and TLR4 genes in both the healthy and RA FLSs compared with that of their control groups, and pretreatment with TAK-242 reversed the effect. Furthermore, LPS-stimulated FLSs significantly increased the level of pIkβα in both the healthy and RA FLSs compared with that of their control groups, and pretreatment with TAK-242 reversed the effect. We provide the data that TAK-242 through inhibiting the NF-κB signaling pathway may modulate TLR4-mediated inflammatory responses and could be considered as a potential therapeutic agent for RA patients.
Keywords	Rheumatoid arthritis ; Trauma and stressor related disorders ; Toll-like receptor 4 ; Medicine ; R
Subject code	616
Language	English
Publishing date	2021-08-01T00:00:00Z
Publisher	Tehran University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Optimization of Cloning Conditions for High-Level Production of Recombinant Mouse Interleukin-2 in Escherichia coli

Arezou Abdi / Mitra Hosseinpour / Kazem Mashayekhi / Mohammad Javad Mousavi / Seyedeh Elham Badiee Kheirabadi / Mojtaba Sankian

Research in Molecular Medicine, Vol 7, Iss 1, Pp 16-

2019 Volume 25

Abstract: Backgrounds Many proteins have been expressed so far in bacterial host. Due to its simple culture conditions, having a short life cycle, and easily genetic manipulation, E.coli have been regarded as a preferable host to produce recombinant proteins, but ... ...

Abstract	Backgrounds Many proteins have been expressed so far in bacterial host. Due to its simple culture conditions, having a short life cycle, and easily genetic manipulation, E.coli have been regarded as a preferable host to produce recombinant proteins, but protein cloning in bacterial host have many challenges. Therefore, we aimed to review some of these problems by an experience from mice IL-2 recombinant. Materials and methods: cDNA synthesis was performed after RNA extraction of mouse splenocytes. PCR product purification carried out after IL-2 coding sequence amplification and was ligated into the pET-21b (+) vector and transformed into the competent BL21 E.coli. Expression and purification of recombinant mouse IL-2 were done using IPTG inducer and metal affinity chromatography respectively. Results: DNA sequencing confirmed the accuracy of the insertion process. A 23 kDa exogenous protein was observed on the SDS-PAGE. Specificity and concentration of produced mouse recombinant IL-2 protein were confirmed by western blotting and BCA methods. Conclusion: Recombinant IL-2 was produced in BL21 and pET-21b (+) expression system at 24°C in the soluble form.
Keywords	Recombinant protein ; pET-21b (+) ; IL-2 protein ; Cloning ; Medicine (General) ; R5-920
Subject code	572
Language	English
Publishing date	2019-02-01T00:00:00Z
Publisher	Mazandaran University of Medical Sciences and Health Services
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Role of Fibroblast Activation Protein Alpha in Fibroblast-like Synoviocytes of Rheumatoid Arthritis

Mohammad Javad Mousavi / Elham Farhadi / Mohammad Vodjgani / Jafar Karami / Mohammad Naghi Tahmasebi / Arash Sharafat Vaziri / Marzieh Asgari / Nima Rezaei / Shayan Mostafaei / Ahmadreza Jamshidi / Mahdi Mahmoudi

Iranian Journal of Allergy, Asthma and Immunology, Vol 20, Iss

2021 Volume 3

Abstract: Fibroblast-like synoviocytes (FLSs) have been introduced in recent years as a key player in the pathogenesis of rheumatoid arthritis (RA), but the exact mechanisms of their transformation and intracellular pathways have not yet been determined. This ... ...

Abstract	Fibroblast-like synoviocytes (FLSs) have been introduced in recent years as a key player in the pathogenesis of rheumatoid arthritis (RA), but the exact mechanisms of their transformation and intracellular pathways have not yet been determined. This study aimed to investigate the role of fibroblast activation protein-alpha (FAP-α) in the regulation of genes involved in the transformation and pathogenic activity of RA FLSs. Synovial FLSs were isolated from RA patients and non-arthritic individuals (n=10 in both groups) and characterized; using immunocytochemistry and flow cytometry analysis. FLSs were divided into un-treated and Talabostat-treated groups to evaluate the FAP-α effect on the selected genes involved in cell cycle regulation (p21, p53, CCND1), apoptosis (Bcl-2, PUMA), and inflammatory and destructive behavior of FLSs (IL-6, TGF-β1, MMP-2, MMP-9, P2RX7). Gene expression analysis was performed by quantitative real-time polymerase chain reaction (qRT-PCR), and immunoblotting was carried out to evaluate FAP-α protein levels. The basal level of FAP-α protein in RA patients was significantly higher than non-arthritic control individuals. However, no differences were observed between RA and non-arthritic FLSs, at the baseline mRNA levels of all the genes. Talabostat treatment significantly reduced FAP-α protein levels in both RA and non-arthritic FLSs, however, had no effect on mRNA expressions except an upregulated TGF-β1 expression in non-arthritic FLSs. A significantly higher protein level of FAP-α in FLSs of RA patients compared with that of healthy individuals may point to the pathogenic role of this protein in RA FLSs. However, more investigations are necessary to address the mechanisms mediating the FAP-α pathogenic role in RA FLSs.
Keywords	Fibroblast activation protein alpha ; Rheumatoid arthritis ; Fibroblast-like synoviocytes ; Medicine ; R
Subject code	616
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	Tehran University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: SARS-Cov-2 and COVID-19, Basic and Clinical Aspects of the Human Pandemic

Mahdi Zavvar / Hamid Emadi Kochak / Kamal Abdolmohammadi / Nesa Rashidi / Majid Mokhtari / Farshid Noorbakhsh / Kayahan Azadmanesh / Ehsan Shamsi Gooshki / Yousef Fatahi / Talat Mokhtari Azad / Alireza Jahangirifard / Mohammad Javad Mousavi / Elham Masoumi / Hamid Reza Mirzaei / Mohammad Mahdi Gouya / Farshid Rezaei / Mohammad Hossein Nicknam

Iranian Journal of Public Health, Vol 50, Iss

A Review

2021 Volume 4

Abstract: In the last two decades, we have witnessed three major epidemics of the coronavirus human disease namely, severe acute respiratory syndrome (SARS), Middle Eastern respiratory syndrome, and more recently an ongoing global pandemic of coronavirus disease ... ...

Abstract	In the last two decades, we have witnessed three major epidemics of the coronavirus human disease namely, severe acute respiratory syndrome (SARS), Middle Eastern respiratory syndrome, and more recently an ongoing global pandemic of coronavirus disease 2019 (COVID-19). Iran, a country of nearly 84 million, in the Middle East, severely involved with the COVID-19 disease. A documented multidimensional approach to COVID-19 disease is therefore mandatory to provide a well-balanced platform for the concerned medical community in our county and beyond. In this review, we highlight the disease status in Iran and attempt to provide a multilateral view of the fundamental and clinical aspects of the disease including the clinical features of the confirmed cases, virology, pathogenesis, epidemiology, and laboratory methods needed for diagnosis.
Keywords	Coronaviruses ; Coronavirus disease 2019 ; COVID-19 ; SARS-CoV-2 ; Public aspects of medicine ; RA1-1270
Subject code	610
Language	English
Publishing date	2021-04-01T00:00:00Z
Publisher	Tehran University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED