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  1. AU="Mohammad Shahrooei"
  2. AU="Brandjes, Dees P M"
  3. AU="Zou, J Z"
  4. AU="Kamal Dahan Alsultan"
  5. AU="Ayres-Silva, Jackline P"
  6. AU="Devrim, Fatma"
  7. AU="Michael J Horzewski"
  8. AU="Mumtaz, Nazia"
  9. AU="Elsa R. Treffeisen"
  10. AU="Ram, Natalie"
  11. AU="Azzaoui, Kamal"
  12. AU="Clowney, Billy"
  13. AU="Andrews, Lawrence C."
  14. AU="Arias, Andrés Hugo"
  15. AU="Yeo, B K"
  16. AU="Sultan, Mahmoud H"
  17. AU="Kou, Z Q"
  18. AU="Lamm, L"
  19. AU="Truxius, Lidia"
  20. AU="Xingyi Guo"
  21. AU="Gang Lin"
  22. AU="Oka, T"
  23. AU="Frank-Pearce, Summer G"
  24. AU="Hairi Li"
  25. AU="Park, SungHee"
  26. AU="Pascual-Carreras, Eudald"
  27. AU=Joffe Ari R
  28. AU="Buccafurri, Francesco"
  29. AU="Naomi H. Philip"
  30. AU="P. Naina"
  31. AU="Sigal, Leonard H"
  32. AU="Xu, T" AU="Xu, T"
  33. AU="Mazlout, Adam"
  34. AU="Novak, Cheryl B"
  35. AU="Ren, Zhongmin"
  36. AU="Nadhira Houhou-Fidouh"
  37. AU="Seiffert, Jacqui"
  38. AU=Zhang Zizhen
  39. AU="Bhupender Singh Negi"

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  1. Artikel ; Online: Characterization of bacterial diversity between two coastal regions with heterogeneous soil texture

    Maryam Zakavi / Hossein Askari / Mohammad Shahrooei

    Scientific Reports, Vol 12, Iss 1, Pp 1-

    2022  Band 14

    Abstract: Abstract Studying microbial diversity and the effects of external factors on the microbiome could expand our understanding of environmental alterations. Silt and sand are mineral particles that form soil texture and even though most of the soils on earth ...

    Abstract Abstract Studying microbial diversity and the effects of external factors on the microbiome could expand our understanding of environmental alterations. Silt and sand are mineral particles that form soil texture and even though most of the soils on earth contain a fraction of them and some other soils form almost by them, their effects on the microbiome remained to elucidate. In this study, the bacterial biodiversity of sand and silt clay soils was investigated. Furthermore, their effects on plant growth have been determined. Our data showed that biodiversity and biomass of microbiome are higher in silt-based soil. It is interesting that the pseudomonas genera only exist in silt-based soil while it is in the absence of sand-based soil. In contrast, B. thuringiensis could be found in sand-based soil while it is not found in silt texture. Our data also demonstrated that there are no significant changes in stress response between the two groups however, differential physiological changes in plants inoculated with silt and sand based bacterial isolates have been observed. This data could indicate that smaller size particles could contain more bacteria with higher biodiversity due to providing more surfaces for bacteria to grow.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 580
    Sprache Englisch
    Erscheinungsdatum 2022-11-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
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  2. Artikel ; Online: Specific IgE Assay for Respiratory Allergens in Patients with Atopy in Ahvaz, Iran

    Maryam Moradi / Abbas Fayezi / Mana Momeni / Asyeh Javanian / Suzan Amini / Mohammad Shahrooei

    Iranian Journal of Immunology, Vol 15, Iss 4, Pp 294-

    2018  Band 301

    Schlagwörter aeroallergen ; allergy ; immunology ; specific ige ; Biology (General) ; QH301-705.5
    Sprache Englisch
    Erscheinungsdatum 2018-12-01T00:00:00Z
    Verlag Shiraz University of Medical Sciences
    Dokumenttyp Artikel ; Online
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  3. Artikel ; Online: Atypical Omenn Syndrome Due to RAG2 Gene Mutation, a Case Report

    Ali Pourvali / Saba Arshi / Mohammad Nabavi / Mohammad Hasan Bemanian / Sima Shokri / Mohammad Shahrooei / Nima Rezaei / Morteza Fallahpour

    Iranian Journal of Immunology, Vol 16, Iss 4, Pp 334-

    2019  Band 338

    Abstract: Severe Combined Immunodeficiency (SCID), characterized by a profound decrease in both the number and function of T cells, is related to more than 20 different mutations. Recombination-activating gene (RAG) 1 and 2 seem to be two of the most common forms ... ...

    Abstract Severe Combined Immunodeficiency (SCID), characterized by a profound decrease in both the number and function of T cells, is related to more than 20 different mutations. Recombination-activating gene (RAG) 1 and 2 seem to be two of the most common forms presenting with various manifestations, including typical SCID, Omenn syndrome (OS), atypical SCID (AS), or delayed onset combined immunodeficiency with granulomas. One interesting manifestation in RAG mutation is the change in the immunophenotype over time, even after hematopoietic stem cell transplantation (HSCT). As bone marrow transplantation (BMT) is the only curative treatment of SCID, it is necessary to differentiate between SCID and OS due to the different conditioning regimens (CR). We present a novel case of atypical SCID (SCID manifestations with more than 300 CD3 + T cells) caused by RAG 2 gene mutation whose immunophenotype changed to atypical Omenn syndrome (all Omenn syndrome manifestations except rash, eosinophilia, and elevated IgE) over time.Differentiation of leaky SCID, SCID and Omenn syndrome in RAG mutation genes and overlap manifestations is important in treatment plan and prognosis.
    Schlagwörter atypical omen syndrome ; atypical scid ; rag 2 mutation ; severe combined immunodeficiency ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2019-12-01T00:00:00Z
    Verlag Shiraz University of Medical Sciences
    Dokumenttyp Artikel ; Online
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  4. Artikel ; Online: Frequency and Pattern of IgE-mediated Sensitization to Aero and Food Allergens in Ahvaz, Province of Khuzestan in Southwestern Iran

    Mohammad Shahrooei / Abbas Fayezi / Raheleh Shokouhi Shoormasti / Faramarz Zakavi / Leila Golpasand Hagh / Johan Van Eldere / Xavier Bossuyt / Erna Van Hoeyveld

    Iranian Journal of Allergy, Asthma and Immunology, Vol 17, Iss

    2018  Band 4

    Abstract: Efficient diagnosis of allergy and proper treatment need identification of the causative allergens eliciting clinical symptoms. The present study was performed to identify the most common aero- and food allergens and determine the pattern of ... ...

    Abstract Efficient diagnosis of allergy and proper treatment need identification of the causative allergens eliciting clinical symptoms. The present study was performed to identify the most common aero- and food allergens and determine the pattern of sensitization among people of Ahvaz (southwestern Iran), one of the most polluted cities worldwide. Based on the physical examination and medical records, patients were referred to the Allergy laboratory for “in vitro” IgE determination. Specific and total IgE was determined by the ImmunoCAP system (Thermo Fisher-Phadia, Uppsala, Sweden). A total of 666 consecutive patients (51.1% female) were tested for 202 different allergens. The majority of requests (57%) belonged to food allergens. Sensitization to at least one allergen was found in 47.6% of patients. In a selected group of allergens for which specific IgE had been tested in at least 100 patients, the most common sensitizing aeroallergens were Russian thistle, grass pollen, and willow; while wheat, honey, and shrimp were the most frequent food allergens, respectively. Sensitization profiles based on measurement of specific IgE indicated that Russian thistle, grasses, and wheat were the most prevalent allergens in people with allergic symptoms living in Ahvaz.
    Schlagwörter Allergen ; Hypersensitivity ; Iran ; Prevalence ; Specific IgE ; Medicine ; R
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2018-08-01T00:00:00Z
    Verlag Tehran University of Medical Sciences
    Dokumenttyp Artikel ; Online
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  5. Artikel ; Online: Exome-first Approach Identified Novel Homozygous Dedicator of Cytokinesis 8 (DOCK8) Mutations in Three Unrelated Iranian Pedigrees Suspected with Hyper-IgE Syndrome

    Ali Aghebati-Maleki / Tina Shahani / Tooba Momen / Soheila Alyasin / Majid Changi-Ashtiani / Alireza Biglari / Mohammad Shahrooei / Asiyeh Sadat Javanian / Suzan Amini / Xavier Bossuyt / Hassan Rokni-Zadeh

    Iranian Journal of Allergy, Asthma and Immunology, Vol 19, Iss

    2020  Band 2

    Abstract: The prevalence of primary immunodeficiency (PID) is rather high in Iran compared to the world average, mainly due to the high rate of consanguineous marriage. Despite that, little genetic information is available about primary immunodeficiencies in Iran. ...

    Abstract The prevalence of primary immunodeficiency (PID) is rather high in Iran compared to the world average, mainly due to the high rate of consanguineous marriage. Despite that, little genetic information is available about primary immunodeficiencies in Iran. Autosomal recessive hyper IgE syndrome (AR-HIES) is a severe type of immunodeficiency, mainly caused by mutations in the dedicator of cytokinesis 8 (DOCK8). Rapid and precise diagnoses of patients suffering from AR-HIES can help to manage the patients and reach properly the treatment decision. However, in regions with low financial resources and limited expertise, deep phenotyping is uncommon. Therefore, an exome-first approach is helpful to make a genetic-based diagnosis. In the present study, whole-exome sequencing (WES) was applied to detect causative mutations in three unrelated primary immunodeficient patients with poor clinical information. One of the cases was a deceased patient with suspected hyper IgE syndrome (HIES) whose parents were subjected to WES. As a result, three novel pathogenic variants were detected in the DOCK8 gene, including two splicing sites (c.4241+1G>T and c.4886+1G>T) and one-stop-gain (c.4201G>T, p.Glu1401Ter) variants. Sanger sequencing confirmed the mutations’ segregation in corresponding families. Further immunological investigations confirmed that HIES in the studied probands. The presence of frontal bossing and broad nose in one of the studied cases, in addition to the typical clinical presentation of DOCK8-AR-HIES, is notable. This work suggests that an exome-first approach can be a valuable alternative strategy for precise diagnosis of primary immunodeficiency patients.
    Schlagwörter Dedicator of cytokinesis 8 ; Exome-first approach ; Hyper IgE syndrome ; Whole exome sequencing ; Medicine ; R
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2020-04-01T00:00:00Z
    Verlag Tehran University of Medical Sciences
    Dokumenttyp Artikel ; Online
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  6. Artikel: Purification and Evaluation of Polysaccharide Intercellular Adhesion (PIA) Antigen from Staphylococcus epidermidis

    Mirzaei, Bahman / Farzam Vaziri / Mohammad Shahrooei / Ryhane Babaei / Seyed Davar Siadat / Seyed Fazlollah Moosavi

    Current microbiology. 2016 Nov., v. 73, no. 5

    2016  

    Abstract: The polysaccharide intercellular adhesin (PIA) confers major functional effects in biofilm formation, which bears an important role in the pathogenicity of Staphylococcus epidermidis. Following the identification of biofilm-forming strains by biochemical ...

    Abstract The polysaccharide intercellular adhesin (PIA) confers major functional effects in biofilm formation, which bears an important role in the pathogenicity of Staphylococcus epidermidis. Following the identification of biofilm-forming strains by biochemical and molecular methods, isogenic strain was prepared and in vitro biofilm formation assay was performed consequently. By parallel analysis of both the PIA-positive and PIA-negative strains using size exclusion chromatography by Fast protein liquid chromatography (FPLC) method, the respective PIA was purified. Recovered PIA was examined using colorimetric and hemagglutination assays. Finally, the recovered PIA was analyzed using Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy methods. By the parallel purification process and comparison of the obtained graphs from the FPLC detector, fractions near the void volume were determined as PIA. The colorimetric and hemagglutination assays were applied and the content of carbohydrates (hexose = 620 μg/ml, hexosamine = 5700 μg/ml and ketoses = 170 μg/ml) and hemagglutination titer (1:128) in recovered polysaccharide were determined. This study shows that PIA has a significant role in the biofilm formation in S. epidermidis strains. The recovered polysaccharide and its molecular weight were analyzed within the near void volume of the utilized column.
    Schlagwörter adhesins ; antigens ; biofilm ; cell adhesion ; colorimetry ; Fourier transform infrared spectroscopy ; gel chromatography ; hemagglutination ; hexosamines ; ketoses ; molecular weight ; nuclear magnetic resonance spectroscopy ; pathogenicity ; polysaccharides ; purification methods ; Staphylococcus epidermidis
    Sprache Englisch
    Erscheinungsverlauf 2016-11
    Umfang p. 611-617.
    Erscheinungsort Springer US
    Dokumenttyp Artikel
    ZDB-ID 134238-1
    ISSN 1432-0991 ; 0343-8651
    ISSN (online) 1432-0991
    ISSN 0343-8651
    DOI 10.1007/s00284-016-1098-5
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  7. Artikel: sesC as a genetic marker for easy identification of Staphylococcus epidermidis from other isolates

    Khodaparast, Ladan / Johan Van Eldere / Laleh Khodaparast / Lieve Van Mellaert / Marc Van Ranst / Mohammad Shahrooei

    Infection, genetics, and evolution. 2016 Sept., v. 43

    2016  

    Abstract: Staphylococcus epidermidis is one of the major concerns with respect to hospital-acquired infections. Therefore, a rapid and easy method to identify at species level S. epidermidis isolates out of a broad range of bacteria is necessary.Based on earlier ... ...

    Abstract Staphylococcus epidermidis is one of the major concerns with respect to hospital-acquired infections. Therefore, a rapid and easy method to identify at species level S. epidermidis isolates out of a broad range of bacteria is necessary.Based on earlier studies, the sesC gene encoding a S. epidermidis surface protein revealed to be a highly conserved gene in this species. By means of an easy and inexpensive PCR assay, the presence of sesC was checked in 438 clinical staphylococcal isolates. Results showed that sesC is specifically present in all S. epidermidis. In conclusion, the sesC gene can be exploited as a genetic marker in order to distinguish S. epidermidis from other isolates.
    Schlagwörter bacteria ; genes ; genetic markers ; polymerase chain reaction ; Staphylococcus epidermidis ; surface proteins
    Sprache Englisch
    Erscheinungsverlauf 2016-09
    Umfang p. 222-224.
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    ZDB-ID 2037068-4
    ISSN 1567-1348
    ISSN 1567-1348
    DOI 10.1016/j.meegid.2016.05.037
    Datenquelle NAL Katalog (AGRICOLA)

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  8. Artikel ; Online: The Possible Role of Staphylococcus epidermidis LPxTG Surface Protein SesC in Biofilm Formation.

    Laleh Khodaparast / Ladan Khodaparast / Mohammad Shahrooei / Benoit Stijlemans / Rita Merckx / Pieter Baatsen / James P O'Gara / Elaine Waters / Lieve Van Mellaert / Johan Van Eldere

    PLoS ONE, Vol 11, Iss 1, p e

    2016  Band 0146704

    Abstract: Staphylococcus epidermidis is the most common cause of device-associated infections. It has been shown that active and passive immunization in an animal model against protein SesC significantly reduces S. epidermidis biofilm-associated infections. In ... ...

    Abstract Staphylococcus epidermidis is the most common cause of device-associated infections. It has been shown that active and passive immunization in an animal model against protein SesC significantly reduces S. epidermidis biofilm-associated infections. In order to elucidate its role, knock-out of sesC or isolation of S. epidermidis sesC-negative mutants were attempted, however, without success. As an alternative strategy, sesC was introduced into Staphylococcus aureus 8325-4 and its isogenic icaADBC and srtA mutants, into the clinical methicillin-sensitive S. aureus isolate MSSA4 and the MRSA S. aureus isolate BH1CC, which all lack sesC. Transformation of these strains with sesC i) changed the biofilm phenotype of strains 8325-4 and MSSA4 from PIA-dependent to proteinaceous even though PIA synthesis was not affected, ii) converted the non-biofilm-forming strain 8325-4 ica::tet to a proteinaceous biofilm-forming strain, iii) impaired PIA-dependent biofilm formation by 8325-4 srtA::tet, iv) had no impact on protein-mediated biofilm formation of BH1CC and v) increased in vivo catheter and organ colonization by strain 8325-4. Furthermore, treatment with anti-SesC antibodies significantly reduced in vitro biofilm formation and in vivo colonization by these transformants expressing sesC. These findings strongly suggest that SesC is involved in S. epidermidis attachment to and subsequent biofilm formation on a substrate.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 616
    Sprache Englisch
    Erscheinungsdatum 2016-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
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  9. Artikel ; Online: Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis

    Ladan Khodaparast / Laleh Khodaparast / Rodrigo Gallardo / Nikolaos N. Louros / Emiel Michiels / Reshmi Ramakrishnan / Meine Ramakers / Filip Claes / Lydia Young / Mohammad Shahrooei / Hannah Wilkinson / Matyas Desager / Wubishet Mengistu Tadesse / K. Peter R. Nilsson / Per Hammarström / Abram Aertsen / Sebastien Carpentier / Johan Van Eldere / Frederic Rousseau /
    Joost Schymkowitz

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Band 15

    Abstract: Aggregation is sequence-specific and nucleated by short aggregating protein segments (APR). Here authors use a multidisciplinary approach to show that in E.coli some frequently occurring APRs lead to protein aggregation and ultimately bacterial cell ... ...

    Abstract Aggregation is sequence-specific and nucleated by short aggregating protein segments (APR). Here authors use a multidisciplinary approach to show that in E.coli some frequently occurring APRs lead to protein aggregation and ultimately bacterial cell death, which could serve as antibacterial strategy.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2018-02-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
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  10. Artikel ; Online: Correction

    Daniela Matuozzo / Estelle Talouarn / Astrid Marchal / Peng Zhang / Jeremy Manry / Yoann Seeleuthner / Yu Zhang / Alexandre Bolze / Matthieu Chaldebas / Baptiste Milisavljevic / Adrian Gervais / Paul Bastard / Takaki Asano / Lucy Bizien / Federica Barzaghi / Hassan Abolhassani / Ahmad Abou Tayoun / Alessandro Aiuti / Ilad Alavi Darazam /
    Luis M. Allende / Rebeca Alonso-Arias / Andrés Augusto Arias / Gokhan Aytekin / Peter Bergman / Simone Bondesan / Yenan T. Bryceson / Ingrid G. Bustos / Oscar Cabrera-Marante / Sheila Carcel / Paola Carrera / Giorgio Casari / Khalil Chaïbi / Roger Colobran / Antonio Condino-Neto / Laura E. Covill / Ottavia M. Delmonte / Loubna El Zein / Carlos Flores / Peter K. Gregersen / Marta Gut / Filomeen Haerynck / Rabih Halwani / Selda Hancerli / Lennart Hammarström / Nevin Hatipoğlu / Adem Karbuz / Sevgi Keles / Christèle Kyheng / Rafael Leon-Lopez / Jose Luis Franco / Davood Mansouri / Javier Martinez-Picado / Ozge Metin Akcan / Isabelle Migeotte / Pierre-Emmanuel Morange / Guillaume Morelle / Andrea Martin-Nalda / Giuseppe Novelli / Antonio Novelli / Tayfun Ozcelik / Figen Palabiyik / Qiang Pan-Hammarström / Rebeca Pérez de Diego / Laura Planas-Serra / Daniel E. Pleguezuelo / Carolina Prando / Aurora Pujol / Luis Felipe Reyes / Jacques G. Rivière / Carlos Rodriguez-Gallego / Julian Rojas / Patrizia Rovere-Querini / Agatha Schlüter / Mohammad Shahrooei / Ali Sobh / Pere Soler-Palacin / Yacine Tandjaoui-Lambiotte / Imran Tipu / Cristina Tresoldi / Jesus Troya / Diederik van de Beek / Mayana Zatz / Pawel Zawadzki / Saleh Zaid Al-Muhsen / Mohammed Faraj Alosaimi / Fahad M. Alsohime / Hagit Baris-Feldman / Manish J. Butte / Stefan N. Constantinescu / Megan A. Cooper / Clifton L. Dalgard / Jacques Fellay / James R. Heath / Yu-Lung Lau / Richard P. Lifton / Tom Maniatis / Trine H. Mogensen / Horst von Bernuth / Alban Lermine / Michel Vidaud / Anne Boland / Jean-François Deleuze / Robert Nussbaum / Amanda Kahn-Kirby / France Mentre / Sarah Tubiana / Guy Gorochov / Florence Tubach / Pierre Hausfater / COVID Human Genetic Effort / COVIDeF Study Group / French COVID Cohort Study Group / CoV-Contact Cohort / COVID Clinicians / Orchestra Working Group / Amsterdam UMC Covid-19 Biobank / NIAID-USUHS COVID Study Group / Isabelle Meyts / Shen-Ying Zhang / Anne Puel / Luigi D. Notarangelo / Stephanie Boisson-Dupuis / Helen C. Su / Bertrand Boisson / Emmanuelle Jouanguy / Jean-Laurent Casanova / Qian Zhang / Laurent Abel / Aurélie Cobat

    Genome Medicine, Vol 16, Iss 1, Pp 1-

    Rare predicted loss-of-function variants of type I IFN immunity genes are associated with life-threatening COVID-19

    2024  Band 3

    Schlagwörter Medicine ; R ; Genetics ; QH426-470
    Sprache Englisch
    Erscheinungsdatum 2024-01-01T00:00:00Z
    Verlag BMC
    Dokumenttyp Artikel ; Online
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