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  1. Article ; Online: Boosting dendritic cell nanovaccines.

    Mohammadzadeh, Yahya / De Palma, Michele

    Nature nanotechnology

    2022  Volume 17, Issue 5, Page(s) 442–444

    MeSH term(s) Cancer Vaccines ; Dendritic Cells ; Immunotherapy ; Nanoparticles
    Chemical Substances Cancer Vaccines
    Language English
    Publishing date 2022-04-12
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 2254964-X
    ISSN 1748-3395 ; 1748-3387
    ISSN (online) 1748-3395
    ISSN 1748-3387
    DOI 10.1038/s41565-022-01089-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Basal Gp78-dependent mitophagy promotes mitochondrial health and limits mitochondrial ROS

    Alan, Parsa / Vandevoorde, Kurt R. / Joshi, Bharat / Cardoen, Ben / Gao, Guang / Mohammadzadeh, Yahya / Hamarneh, Ghassan / Nabi, Ivan R.

    Cell. Mol. Life Sci.. 2022 Nov., v. 79, no. 11 p.565-565

    2022  

    Abstract: Mitochondria are major sources of cytotoxic reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, that when uncontrolled contribute to cancer progression. Maintaining a finely tuned, healthy mitochondrial population is essential for ... ...

    Abstract Mitochondria are major sources of cytotoxic reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, that when uncontrolled contribute to cancer progression. Maintaining a finely tuned, healthy mitochondrial population is essential for cellular homeostasis and survival. Mitophagy, the selective elimination of mitochondria by autophagy, monitors and maintains mitochondrial health and integrity, eliminating damaged ROS-producing mitochondria. However, mechanisms underlying mitophagic control of mitochondrial homeostasis under basal conditions remain poorly understood. E3 ubiquitin ligase Gp78 is an endoplasmic reticulum membrane protein that induces mitochondrial fission and mitophagy of depolarized mitochondria. Here, we report that CRISPR/Cas9 knockout of Gp78 in HT-1080 fibrosarcoma cells increased mitochondrial volume, elevated ROS production and rendered cells resistant to carbonyl cyanide m-chlorophenyl hydrazone (CCCP)-induced mitophagy. These effects were phenocopied by knockdown of the essential autophagy protein ATG5 in wild-type HT-1080 cells. Use of the mito-Keima mitophagy probe confirmed that Gp78 promoted both basal and damage-induced mitophagy. Application of a spot detection algorithm (SPECHT) to GFP-mRFP tandem fluorescent-tagged LC3 (tfLC3)-positive autophagosomes reported elevated autophagosomal maturation in wild-type HT-1080 cells relative to Gp78 knockout cells, predominantly in proximity to mitochondria. Mitophagy inhibition by either Gp78 knockout or ATG5 knockdown reduced mitochondrial potential and increased mitochondrial ROS. Live cell analysis of tfLC3 in HT-1080 cells showed the preferential association of autophagosomes with mitochondria of reduced potential. Xenograft tumors of HT-1080 knockout cells show increased labeling for mitochondria and the cell proliferation marker Ki67 and reduced labeling for the TUNEL cell death reporter. Basal Gp78-dependent mitophagic flux is, therefore, selectively associated with reduced potential mitochondria promoting maintenance of a healthy mitochondrial population, limiting ROS production and tumor cell proliferation.
    Keywords CRISPR-Cas systems ; algorithms ; autophagosomes ; cell proliferation ; cyanides ; cytotoxicity ; endoplasmic reticulum ; fibrosarcoma ; homeostasis ; hydrazones ; hydrogen peroxide ; membrane proteins ; mitochondria ; mitophagy ; neoplasm cells ; neoplasm progression ; ubiquitin-protein ligase ; xenotransplantation
    Language English
    Dates of publication 2022-11
    Size p. 565.
    Publishing place Springer International Publishing
    Document type Article ; Online
    ZDB-ID 1358415-7
    ISSN 1420-9071 ; 1420-682X
    ISSN (online) 1420-9071
    ISSN 1420-682X
    DOI 10.1007/s00018-022-04585-8
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  3. Article ; Online: Basal Gp78-dependent mitophagy promotes mitochondrial health and limits mitochondrial ROS.

    Alan, Parsa / Vandevoorde, Kurt R / Joshi, Bharat / Cardoen, Ben / Gao, Guang / Mohammadzadeh, Yahya / Hamarneh, Ghassan / Nabi, Ivan R

    Cellular and molecular life sciences : CMLS

    2022  Volume 79, Issue 11, Page(s) 565

    Abstract: Mitochondria are major sources of cytotoxic reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, that when uncontrolled contribute to cancer progression. Maintaining a finely tuned, healthy mitochondrial population is essential for ... ...

    Abstract Mitochondria are major sources of cytotoxic reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, that when uncontrolled contribute to cancer progression. Maintaining a finely tuned, healthy mitochondrial population is essential for cellular homeostasis and survival. Mitophagy, the selective elimination of mitochondria by autophagy, monitors and maintains mitochondrial health and integrity, eliminating damaged ROS-producing mitochondria. However, mechanisms underlying mitophagic control of mitochondrial homeostasis under basal conditions remain poorly understood. E3 ubiquitin ligase Gp78 is an endoplasmic reticulum membrane protein that induces mitochondrial fission and mitophagy of depolarized mitochondria. Here, we report that CRISPR/Cas9 knockout of Gp78 in HT-1080 fibrosarcoma cells increased mitochondrial volume, elevated ROS production and rendered cells resistant to carbonyl cyanide m-chlorophenyl hydrazone (CCCP)-induced mitophagy. These effects were phenocopied by knockdown of the essential autophagy protein ATG5 in wild-type HT-1080 cells. Use of the mito-Keima mitophagy probe confirmed that Gp78 promoted both basal and damage-induced mitophagy. Application of a spot detection algorithm (SPECHT) to GFP-mRFP tandem fluorescent-tagged LC3 (tfLC3)-positive autophagosomes reported elevated autophagosomal maturation in wild-type HT-1080 cells relative to Gp78 knockout cells, predominantly in proximity to mitochondria. Mitophagy inhibition by either Gp78 knockout or ATG5 knockdown reduced mitochondrial potential and increased mitochondrial ROS. Live cell analysis of tfLC3 in HT-1080 cells showed the preferential association of autophagosomes with mitochondria of reduced potential. Xenograft tumors of HT-1080 knockout cells show increased labeling for mitochondria and the cell proliferation marker Ki67 and reduced labeling for the TUNEL cell death reporter. Basal Gp78-dependent mitophagic flux is, therefore, selectively associated with reduced potential mitochondria promoting maintenance of a healthy mitochondrial population, limiting ROS production and tumor cell proliferation.
    MeSH term(s) Humans ; Mitophagy ; Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology ; Reactive Oxygen Species/metabolism ; Ki-67 Antigen/metabolism ; Superoxides/metabolism ; Hydrogen Peroxide/pharmacology ; Mitochondria/metabolism ; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/metabolism ; Autophagy/genetics
    Chemical Substances Carbonyl Cyanide m-Chlorophenyl Hydrazone (555-60-2) ; Reactive Oxygen Species ; Ki-67 Antigen ; Superoxides (11062-77-4) ; Hydrogen Peroxide (BBX060AN9V) ; Ubiquitin-Protein Ligases (EC 2.3.2.27)
    Language English
    Publishing date 2022-10-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1358415-7
    ISSN 1420-9071 ; 1420-682X
    ISSN (online) 1420-9071
    ISSN 1420-682X
    DOI 10.1007/s00018-022-04585-8
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 195: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Cytokine-armed dendritic cell progenitors for antigen-agnostic cancer immunotherapy.

    Ghasemi, Ali / Martinez-Usatorre, Amaia / Li, Luqing / Hicham, Mehdi / Guichard, Alan / Marcone, Rachel / Fournier, Nadine / Torchia, Bruno / Martinez Bedoya, Darel / Davanture, Suzel / Fernández-Vaquero, Mirian / Fan, Chaofan / Janzen, Jakob / Mohammadzadeh, Yahya / Genolet, Raphael / Mansouri, Nahal / Wenes, Mathias / Migliorini, Denis / Heikenwalder, Mathias /
    De Palma, Michele

    Nature cancer

    2023  Volume 5, Issue 2, Page(s) 240–261

    Abstract: Dendritic cells (DCs) are antigen-presenting myeloid cells that regulate T cell activation, trafficking and function. Monocyte-derived DCs pulsed with tumor antigens have been tested extensively for therapeutic vaccination in cancer, with mixed clinical ... ...

    Abstract Dendritic cells (DCs) are antigen-presenting myeloid cells that regulate T cell activation, trafficking and function. Monocyte-derived DCs pulsed with tumor antigens have been tested extensively for therapeutic vaccination in cancer, with mixed clinical results. Here, we present a cell-therapy platform based on mouse or human DC progenitors (DCPs) engineered to produce two immunostimulatory cytokines, IL-12 and FLT3L. Cytokine-armed DCPs differentiated into conventional type-I DCs (cDC1) and suppressed tumor growth, including melanoma and autochthonous liver models, without the need for antigen loading or myeloablative host conditioning. Tumor response involved synergy between IL-12 and FLT3L and was associated with natural killer and T cell infiltration and activation, M1-like macrophage programming and ischemic tumor necrosis. Antitumor immunity was dependent on endogenous cDC1 expansion and interferon-γ signaling but did not require CD8
    MeSH term(s) Humans ; Mice ; Animals ; Cytokines ; Immunotherapy ; Dendritic Cells ; Neoplasms/therapy ; Interleukin-12
    Chemical Substances Cytokines ; Interleukin-12 (187348-17-0)
    Language English
    Publishing date 2023-11-23
    Publishing country England
    Document type Journal Article
    ISSN 2662-1347
    ISSN (online) 2662-1347
    DOI 10.1038/s43018-023-00668-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: A novel chimeric influenza virosome containing Vesicular stomatitis G protein as a more efficient gene delivery system

    Mohammadzadeh, Yahya / Narges Rasouli / Mohammad Hasan Samiee Aref / Nasim Sadat Seyed Tabib / Asghar Abdoli / Peyvand Biglari / Maryam Saleh / Mansoureh Tabatabaeian / Masoumeh Tavassoti Kheiri / Abbas Jamali

    Biotechnology letters. 2016 Aug., v. 38, no. 8

    2016  

    Abstract: OBJECTIVES: To enhance the efficiency of influenza virosome-mediated gene delivery by engineering this virosome. RESULTS: A novel chimeric influenza virosome was constructed containing the glycoprotein of Vesicular stomatitis virus (VSV-G), along with ... ...

    Abstract OBJECTIVES: To enhance the efficiency of influenza virosome-mediated gene delivery by engineering this virosome. RESULTS: A novel chimeric influenza virosome was constructed containing the glycoprotein of Vesicular stomatitis virus (VSV-G), along with its own hemagglutinin protein. To optimize the transfection efficiency of both chimeric and influenza cationic virosomes, HEK cells were transfected with plasmid DNA and virosomes and the transfection efficiency was assessed by FACS analysis. The chimeric virosome was significantly more efficient in mediating transfection for all amounts of DNA and virosomes compared to the influenza virosome. CONCLUSIONS: Chimeric influenza virosome, including VSV-G, is superior to the conventional influenza virosome for gene delivery.
    Keywords Vesiculovirus ; chimerism ; engineering ; glycoproteins ; hemagglutinins ; influenza ; plasmids ; transfection ; vesicular stomatitis
    Language English
    Dates of publication 2016-08
    Size p. 1321-1329.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 423853-9
    ISSN 1573-6776 ; 0141-5492
    ISSN (online) 1573-6776
    ISSN 0141-5492
    DOI 10.1007/s10529-016-2108-1
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4564: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Introduction of cationic virosome derived from vesicular stomatitis virus as a novel gene delivery system for sf9 cells.

    Mohammadzadeh, Yahya / Gholami, Shima / Rasouli, Narges / Sarrafzadeh, Sahar / Seyed Tabib, Nasim Sadat / Samiee Aref, Mohammad Hasan / Abdoli, Asghar / Biglari, Peyvand / Fotouhi, Fatemeh / Farahmand, Behrokh / Tavassoti Kheiri, Masoumeh / Jamali, Abbas

    Journal of liposome research

    2017  Volume 27, Issue 2, Page(s) 83–89

    Abstract: Insect-derived cell lines are used extensively to produce recombinant proteins because they are capable of performing a range of post-translational modifications. Due to their significance in biotechnological applications, various methods have been ... ...

    Abstract Insect-derived cell lines are used extensively to produce recombinant proteins because they are capable of performing a range of post-translational modifications. Due to their significance in biotechnological applications, various methods have been developed to transfect them. In this study, we introduce a virosome constructed from vesicular stomatitis virus (VSV) as a new delivery system for sf9 cells. We labeled these VSV virosomes by fluorescent probe Rhodamine B chloride (R18). By fluorescence microscope observation and conducting a fusion assay, we confirmed the uptake of VSV virosomes via endocytosis by sf9 cells and their fusion with the endosomal membrane. Moreover, we incubated cationic VSV virosomes with a GFP-expressing bacmid and transfected sf9 cells, after 24 h some cells expressed GFP indicating the ability of VSV virosomes to deliver heterologous DNA to these cells. This is the first report of a virosome-based delivery system introduced for an insect cell line.
    MeSH term(s) Animals ; Cations/chemistry ; Cells, Cultured ; Gene Transfer Techniques ; Sf9 Cells ; Spodoptera ; Vesicular stomatitis Indiana virus/chemistry ; Virosomes/chemistry
    Chemical Substances Cations ; Virosomes
    Language English
    Publishing date 2017-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 645173-1
    ISSN 1532-2394 ; 0898-2104
    ISSN (online) 1532-2394
    ISSN 0898-2104
    DOI 10.3109/08982104.2016.1144205
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2539: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: A novel chimeric influenza virosome containing Vesicular stomatitis G protein as a more efficient gene delivery system.

    Mohammadzadeh, Yahya / Rasouli, Narges / Aref, Mohammad Hasan Samiee / Tabib, Nasim Sadat Seyed / Abdoli, Asghar / Biglari, Peyvand / Saleh, Maryam / Tabatabaeian, Mansoureh / Kheiri, Masoumeh Tavassoti / Jamali, Abbas

    Biotechnology letters

    2016  Volume 38, Issue 8, Page(s) 1321–1329

    Abstract: Objectives: To enhance the efficiency of influenza virosome-mediated gene delivery by engineering this virosome.: Results: A novel chimeric influenza virosome was constructed containing the glycoprotein of Vesicular stomatitis virus (VSV-G), along ... ...

    Abstract Objectives: To enhance the efficiency of influenza virosome-mediated gene delivery by engineering this virosome.
    Results: A novel chimeric influenza virosome was constructed containing the glycoprotein of Vesicular stomatitis virus (VSV-G), along with its own hemagglutinin protein. To optimize the transfection efficiency of both chimeric and influenza cationic virosomes, HEK cells were transfected with plasmid DNA and virosomes and the transfection efficiency was assessed by FACS analysis. The chimeric virosome was significantly more efficient in mediating transfection for all amounts of DNA and virosomes compared to the influenza virosome.
    Conclusions: Chimeric influenza virosome, including VSV-G, is superior to the conventional influenza virosome for gene delivery.
    MeSH term(s) Gene Transfer Techniques ; Transfection ; Vesicular Stomatitis/metabolism ; Viral Proteins/genetics ; Viral Proteins/metabolism ; Virosomes/genetics ; Virosomes/metabolism
    Chemical Substances Viral Proteins ; Virosomes
    Language English
    Publishing date 2016-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 423853-9
    ISSN 1573-6776 ; 0141-5492
    ISSN (online) 1573-6776
    ISSN 0141-5492
    DOI 10.1007/s10529-016-2108-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4564: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article: A novel chimeric influenza virosome containing Vesicular stomatitis G protein as a more efficient gene delivery system

    Mohammadzadeh, Yahya / Narges Rasouliauthor / Mohammad Hasan Samiee Arefauthor / Nasim Sadat Seyed Tabibauthor / Asghar Abdoliauthor / Peyvand Biglariauthor / Maryam Salehauthor / Mansoureh Tabatabaeianauthor / Masoumeh Tavassoti Kheiriauthor / Abbas Jamaliauthor
    Language English
    Document type Article
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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