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Article ; Online: Development of newly synthesised quinazolinone-based CDK2 inhibitors with potent efficacy against melanoma.

Mohammed, Eman R / Elmasry, Ghada F

Journal of enzyme inhibition and medicinal chemistry

2022 Volume 37, Issue 1, Page(s) 686–700

Abstract: Inhibiting Cyclin-dependent kinase 2 (CDK2) has been established as a therapeutic strategy for the treatment of many cancers. Accordingly, this study aimed at developing a new set of quinazolinone-based derivatives as CDK2 inhibitors. The new compounds ... ...

Abstract	Inhibiting Cyclin-dependent kinase 2 (CDK2) has been established as a therapeutic strategy for the treatment of many cancers. Accordingly, this study aimed at developing a new set of quinazolinone-based derivatives as CDK2 inhibitors. The new compounds were evaluated for their anticancer activity against sixty tumour cell lines. Compounds
MeSH term(s)	Antineoplastic Agents/chemical synthesis ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Cell Cycle/drug effects ; Cell Line ; Cell Proliferation/drug effects ; Cyclin-Dependent Kinase 2/antagonists & inhibitors ; Cyclin-Dependent Kinase 2/metabolism ; Dose-Response Relationship, Drug ; Drug Development ; Drug Screening Assays, Antitumor ; Humans ; Melanoma/drug therapy ; Melanoma/metabolism ; Melanoma/pathology ; Molecular Docking Simulation ; Molecular Structure ; Protein Kinase Inhibitors/chemical synthesis ; Protein Kinase Inhibitors/chemistry ; Protein Kinase Inhibitors/pharmacology ; Quinazolinones/chemical synthesis ; Quinazolinones/chemistry ; Quinazolinones/pharmacology ; Structure-Activity Relationship
Chemical Substances	Antineoplastic Agents ; Protein Kinase Inhibitors ; Quinazolinones ; CDK2 protein, human (EC 2.7.11.22) ; Cyclin-Dependent Kinase 2 (EC 2.7.11.22)
Language	English
Publishing date	2022-02-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2082578-X
ISSN	1475-6374 ; 1475-6366
ISSN (online)	1475-6374
ISSN	1475-6366
DOI	10.1080/14756366.2022.2036985
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Article ; Online: First molecular identification and phylogenetic illustration of Sarcocystis species infection in Red Sea shortfin mako shark (Isurus oxyrinchus Rafinesque, 1810).

Ahmed, Nahla He M / Ghallab, Ahmed / Shaalan, Mohamed / Saied, Mahmoud / Mohammed, Eman Sayed

BMC veterinary research

2024 Volume 20, Issue 1, Page(s) 104

Abstract: Background: members of the genus Sarcocystis are intracellular obligate protozoan parasites classified within the phylum Apicomplexa and have an obligate heteroxenous life cycle involving two hosts. A more comprehensive understanding of the prevalence ... ...

Abstract	Background: members of the genus Sarcocystis are intracellular obligate protozoan parasites classified within the phylum Apicomplexa and have an obligate heteroxenous life cycle involving two hosts. A more comprehensive understanding of the prevalence and geographic range of different Sarcocystis species in marine ecosystems is needed globally and nationally. Hence, the objective of this study was to document the incidence of Sarcocystis infection in sharks within the aquarium ecosystem of Egypt and to identify the species through the characterization of the SSU rDNA gene. Methods: All organs of the mako shark specimen underwent macroscopic screening to detect the existence of a Sarcocystis cyst. Ten cysts were collected from the intestine and processed separately to extract the genomic DNA. The polymerase chain reaction (PCR) was accomplished by amplifying a specific 18S ribosomal RNA (rRNA) gene fragment. Subsequently, the resulting amplicons were subjected to purification and sequencing processes. Results: Macroscopic examination of the mako shark intestinal wall sample revealed the presence of Sarcocystis cysts of various sizes and shapes, and sequencing of the amplicons from Sarcocystis DNA revealed a 100% nucleotide identity with the sequence of Sarcocystis tenella recorded from sheep in Iran; The mako shark sequence has been deposited in the GeneBank with the accession number OQ721979. This study presents the first scientific evidence demonstrating the presence of the Sarcocystis parasite in sharks, thereby documenting this specific marine species as a novel intermediate host in the Sarcocystis life cycle. Conclusions: This is the first identification of Sarcocystis infection in sharks, and we anticipate it will be an essential study for future screenings and establishing effective management measures for this disease in aquatic ecosystems.
MeSH term(s)	Animals ; Sheep/genetics ; Sarcocystis/genetics ; Ecosystem ; Sharks/genetics ; Phylogeny ; Indian Ocean ; DNA, Ribosomal ; Life Cycle Stages
Chemical Substances	DNA, Ribosomal
Language	English
Publishing date	2024-03-15
Publishing country	England
Document type	Journal Article
ZDB-ID	2191675-5
ISSN	1746-6148 ; 1746-6148
ISSN (online)	1746-6148
ISSN	1746-6148
DOI	10.1186/s12917-024-03952-w
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Article ; Online: Exploring new quinazolin-4(3H)-one derivatives as CDK2 inhibitors: Design, synthesis, and anticancer evaluation.

Ibrahim, Basant T / Allam, Heba Abdelrasheed / El-Dydamony, Nehad M / Fouad, Marwa A / Mohammed, Eman R

Drug development research

2024 Volume 85, Issue 2, Page(s) e22163

Abstract: In the present work, five series of new 2,3-disubstituted quinazolin-4(3H)-ones 4a-c, 5a-d, 6a-g, 7a,b, and 9a-c were designed, synthesized, and screened in vitro for their cytotoxic activity against 60 cancer cell lines by the National Cancer Institute, ...

Abstract	In the present work, five series of new 2,3-disubstituted quinazolin-4(3H)-ones 4a-c, 5a-d, 6a-g, 7a,b, and 9a-c were designed, synthesized, and screened in vitro for their cytotoxic activity against 60 cancer cell lines by the National Cancer Institute, USA. Five candidates 4c, 6a, 6b, 6d, and 6g revealed promising cytotoxicity with significant percentage growth inhibition in the range of 81.98%-96.45% against the central nervous system (CNS) (SNB-19), melanoma (MDA-MB-435), and non-small cell lung cancer (HOP-62) cell lines. The in vitro cytotoxic half maximal inhibitory concentration (IC
MeSH term(s)	Humans ; Structure-Activity Relationship ; Cell Line, Tumor ; Carcinoma, Non-Small-Cell Lung/drug therapy ; Molecular Docking Simulation ; Melanoma/drug therapy ; Drug Screening Assays, Antitumor ; Cell Proliferation ; Lung Neoplasms ; Antineoplastic Agents/chemistry ; Molecular Structure ; Protein Kinase Inhibitors/pharmacology ; Cyclin-Dependent Kinase 2/metabolism
Chemical Substances	Antineoplastic Agents ; Protein Kinase Inhibitors ; CDK2 protein, human (EC 2.7.11.22) ; Cyclin-Dependent Kinase 2 (EC 2.7.11.22)
Language	English
Publishing date	2024-02-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	604587-x
ISSN	1098-2299 ; 0272-4391
ISSN (online)	1098-2299
ISSN	0272-4391
DOI	10.1002/ddr.22163
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Article ; Online: Prevalence and first molecular identification of Sarcocystis species in feces of domestic dogs (Canis familiaris) in Egypt.

Elshahawy, Ismail S / Fawaz, Marwa / Gomaa, Aya / Mohammed, Eman

BMC veterinary research

2023 Volume 19, Issue 1, Page(s) 278

Abstract: Background: Sarcocystis species are obligatorily heteroxenous protozoan parasites with predator-prey life cycles. Global Knowledge about the epidemiology and the distribution pattern of different Sarcocystis species in dog feces are very scarce. ... ...

Abstract	Background: Sarcocystis species are obligatorily heteroxenous protozoan parasites with predator-prey life cycles. Global Knowledge about the epidemiology and the distribution pattern of different Sarcocystis species in dog feces are very scarce. Therefore, the current investigation was conducted to declare the occurrence of Sarcocystis in the fecal specimens of the most common canids in Egypt, the domestic dogs, and to identify the species present using various parasitological and molecular approaches. Methods: A total of 100 dog fecal samples were collected and screened using fecal sugar flotation test for the presence of Sarcocystis oocysts/sporocysts. Additionally, thirty samples were used for genomic DNA extraction. The 18S rRNA gene fragment was the target of primers for a PCR, followed by purification and sequencing of the amplicons. Results: Currently, the results obtained reviewed that 4% of fecal samples were positive for Sarcocystis spp. using LM. Additionally, Sarcocystis spp. were verified in sixteen dogs (53.3%, 16/30) using PCR and subsequent sequencing protocols. Statistically, insignificant difference in prevalence of sarcocystosis relative to age and gender was noticed. Morphologically, the detected sporocysts measured 13.2-16.0 × 9.4-11 μm. Based on the 18S rRNA gene, sequencing analysis of amplicons from sporocysts DNA revealed 99.82% nucleotide homology with published S. tenella partial nucleotide sequences from sheep in Iraq and Iran. Conclusions: This is the first molecular evidence in support of the final host role of domestic dogs in the life cycle of S. tenella in Egypt, which provides a precious diagnostic tool for further epidemiological studies and for the assessment of the effectiveness of control measures for this disease.
MeSH term(s)	Animals ; Dogs ; Sheep/genetics ; Sarcocystis/genetics ; Egypt/epidemiology ; Prevalence ; Sarcocystosis/epidemiology ; Sarcocystosis/veterinary ; Sarcocystosis/parasitology ; DNA, Ribosomal/genetics ; Oocysts ; Feces/parasitology ; RNA, Ribosomal, 18S/genetics ; Phylogeny ; Dog Diseases/epidemiology ; Dog Diseases/parasitology ; Sheep Diseases/parasitology
Chemical Substances	DNA, Ribosomal ; RNA, Ribosomal, 18S
Language	English
Publishing date	2023-12-18
Publishing country	England
Document type	Journal Article
ZDB-ID	2191675-5
ISSN	1746-6148 ; 1746-6148
ISSN (online)	1746-6148
ISSN	1746-6148
DOI	10.1186/s12917-023-03841-8
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Article: Comparison between levels of Homocysteine, Interleukin-6 and Interleukin-10 in severe cases of Egyptian COVID-19 patients.

Abdalwahed, Wafaa A / Mohammed, Eman F / Shipl, Walaa M / Mabrouk, Fatma M

The Egyptian journal of immunology

2023 Volume 30, Issue 3, Page(s) 110–123

Abstract: Coronavirus disease 2019 (COVID-19) is the reason of an outbreak of respiratory illnesses ranging from typical cold to severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome. We intended to compare levels of IL-6, IL-10, and ... ...

Abstract	Coronavirus disease 2019 (COVID-19) is the reason of an outbreak of respiratory illnesses ranging from typical cold to severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome. We intended to compare levels of IL-6, IL-10, and homocysteine in the sera of severe COVID-19 Egyptian patients and connect them with the extent of the illness. This cross-sectional study included 90 COVID-19 Egyptian patients. They included 45 non severe cases (group 1) and 45 severe cases (group 2). There was statistically significantly increase in IL-6 in group 2 (median = 7.05, IQR = 6.2 - 7.9; p < 0.001) in comparison to group 1 (median = 4.96, IQR = 4.5 - 5.8) and statistically significantly increase in IL-10 in group 2 (154.1 ± 73.3) in comparison to group 1 (47.04 ± 23.8; p < 0.001). There were no variations in the examined groups' homocysteine levels (p= 0.318). Furthermore, there was statistically substantial positive connection (r=0.30) between IL-6 and AST (p=0.046) and between IL-10 and HCT (r = 0.37, p=0.012). In addition, data of other studied parameters are presented. In conclusion, IL-6 and IL-10 could be proposed for follow up of COVID-19 patients and to detect cases before progressing to a severe disease stage.
MeSH term(s)	Humans ; COVID-19 ; Interleukin-6 ; Interleukin-10 ; Homocysteine ; Cross-Sectional Studies ; Egypt ; SARS-CoV-2
Chemical Substances	Interleukin-6 ; Interleukin-10 (130068-27-8) ; Homocysteine (0LVT1QZ0BA)
Language	English
Publishing date	2023-07-13
Publishing country	Egypt
Document type	Journal Article
ISSN	1110-4902
ISSN	1110-4902
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Article: Phylogenetic groups, serogroups and virulence factors of uropathogenic

Mohammed, Eman Jassim / Hasan, Kadhim Ch / Allami, Mohammed

Iranian journal of microbiology

2022 Volume 14, Issue 4, Page(s) 445–457

Abstract: Background and objectives: Uropathogenic : Materials and methods: Of the 412 urine samples tested a total of 150 UPEC were isolated and confirmed with PCR using 16S rRNA gene. Antibiotic resistance of the isolates was tested using disk diffusion ... ...

Abstract	Background and objectives: Uropathogenic Materials and methods: Of the 412 urine samples tested a total of 150 UPEC were isolated and confirmed with PCR using 16S rRNA gene. Antibiotic resistance of the isolates was tested using disk diffusion method and the isolates were divided into phylogenetic groups by the quadruplex PCR method. The prevalence of serogroups and virulence genes were investigated using multiplex PCR. Results: 87 (58%) of the isolates belonged to phylogroup B2. Virulence genes Conclusion: The high abundance of phylogenetic group B2, serogroups O8 and O25, and virulence genes
Language	English
Publishing date	2022-12-21
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2652849-6
ISSN	2008-4447 ; 2008-3289
ISSN (online)	2008-4447
ISSN	2008-3289
DOI	10.18502/ijm.v14i4.10230
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Article ; Online: Anticonvulsant Classes and Possible Mechanism of Actions.

Abd-Allah, Walaa Hamada / El-Mohsen Anwar, Mostafa Abd / Mohammed, Eman R / El Moghazy, Samir M

ACS chemical neuroscience

2023 Volume 14, Issue 23, Page(s) 4076–4092

Abstract: Epilepsy is considered one of the most common neurological disorders worldwide; it needs long-term or life-long treatment. Despite the presence of several novel antiepileptic drugs, approximately 30% patients still suffer from drug-resistant epilepsy. ... ...

Abstract	Epilepsy is considered one of the most common neurological disorders worldwide; it needs long-term or life-long treatment. Despite the presence of several novel antiepileptic drugs, approximately 30% patients still suffer from drug-resistant epilepsy. Subsequently, searching for new anticonvulsants with lower toxicity and better efficacy is still in paramount demand. Using target-based studies in the discovery of novel antiepileptics is uncommon owing to the insufficient information on the molecular pathway of epilepsy and complex mode of action for most of known antiepileptic drugs. In this review, we investigated the properties of anticonvulsants, types of epileptic seizures, and mechanism of action for anticonvulsants.
MeSH term(s)	Humans ; Anticonvulsants/pharmacology ; Anticonvulsants/therapeutic use ; Epilepsy/drug therapy ; Epilepsy/chemically induced ; Seizures/drug therapy ; Seizures/chemically induced ; Drug Resistant Epilepsy/drug therapy
Chemical Substances	Anticonvulsants
Language	English
Publishing date	2023-11-10
Publishing country	United States
Document type	Journal Article ; Review
ISSN	1948-7193
ISSN (online)	1948-7193
DOI	10.1021/acschemneuro.3c00613
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Article ; Online: Author Correction: Circulating ESR1, long non-coding RNA HOTAIR and microRNA-130a gene expression as biomarkers for breast cancer stage and metastasis.

Abdel-Hamid, Noura R / Mohammed, Eman A / Toraih, Eman A / Kamel, Mahmoud M / Abdelhafiz, Ahmed Samir / Badr, Fouad M

Scientific reports

2024 Volume 14, Issue 1, Page(s) 2773

Language	English
Publishing date	2024-02-02
Publishing country	England
Document type	Published Erratum
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-52878-8
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Article ; Online: Metabolomic profiling and quantification of polyphenols from leaves of seven Acacia species by UHPLC-QTOF-ESI-MS.

Elshamy, Salma / Handoussa, Heba / El-Shazly, Mohamed / Mohammed, Eman D / Kuhnert, Nikolai

Fitoterapia

2023 Volume 172, Page(s) 105741

Abstract: The genus Acacia (Fabaceae) comprises >1350 species and has been used in traditional medicine as infusions and decoctions to treat wounds, sores, headaches, diarrhea, and cough. The leaf methanolic extracts of seven Acacia species growing in Egypt namely: ...

Abstract	The genus Acacia (Fabaceae) comprises >1350 species and has been used in traditional medicine as infusions and decoctions to treat wounds, sores, headaches, diarrhea, and cough. The leaf methanolic extracts of seven Acacia species growing in Egypt namely: Acacia saligna, Acacia seyal, Acacia xanthophloea, Acacia tortilis subsp. raddiana., Acacia tortilis, Acacia laeta, Acacia albida were analyzed using UPLC-QTOF-ESI-MS. A total of 37 polyphenols were identified and discussed in detail. They included phenolic acids, flavonoids, and procyanidins, among which sixteen polyphenols were identified in Acacia for the first time. Folin-ciocalteau assay and ferric reducing antioxidant power, cupric reducing antioxidant capacity, 2,20 -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) cation radical and the scavenging capacity against 2,2-diphenyl-1- picrylhydrazyl radical were performed to investigate the total phenolic content and the antioxidant activity of the Acacia extracts, respectively. Furthermore, the absolute quantification of eighteen polyphenols common to most of the species was performed using UPLC-MS. It was evident that the differences in the chemical composition among the species accounted for the difference in antioxidant activity which was in line together with the total phenolic content.
MeSH term(s)	Polyphenols/chemistry ; Antioxidants/chemistry ; Chromatography, High Pressure Liquid ; Acacia/chemistry ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Molecular Structure ; Flavonoids/chemistry ; Phenols/chemistry ; Plant Extracts/chemistry ; Plant Leaves/chemistry
Chemical Substances	Polyphenols ; Antioxidants ; Flavonoids ; Phenols ; Plant Extracts
Language	English
Publishing date	2023-11-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	412385-2
ISSN	1873-6971 ; 0367-326X
ISSN (online)	1873-6971
ISSN	0367-326X
DOI	10.1016/j.fitote.2023.105741
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Article ; Online: Zinc Oxide Nanoparticles and Vitamin C Ameliorate Atrazine-Induced Hepatic Apoptosis in Rat via CYP450s/ROS Pathway and Immunomodulation.

Mohammed, Eman T / Safwat, Ghada M / Bahnasawy, Esraa A / Abdel-Razik, Abdel-Razik H / Mohamed, Doaa Sh

Biological trace element research

2023 Volume 201, Issue 11, Page(s) 5257–5271

Abstract: Atrazine, as an herbicide, is used widely worldwide. Because of its prolonged persistence in the environment and accumulation in the body, atrazine exposure is a potential threat to human health. The present study evaluated the possible protective ... ...

Abstract	Atrazine, as an herbicide, is used widely worldwide. Because of its prolonged persistence in the environment and accumulation in the body, atrazine exposure is a potential threat to human health. The present study evaluated the possible protective effects of zinc oxide nanoparticles and vitamin C against atrazine-induced hepatotoxicity in rats. Atrazine administered to rats orally at a dose of 300 mg/kg for 21 days caused liver oxidative stress as it increased malondialdehyde (MDA) formation and decreased reduced glutathione (GSH) contents. Atrazine induced inflammation accompanied by apoptosis via upregulation of hepatic gene expression levels of NF-κB, TNF-α, BAX, and caspase-3 and downregulation of Bcl-2 gene expression levels. Additionally, it disturbed the metabolic activities of cytochrome P450 as it downregulated hepatic gene expression levels of CYP1A1, CYP1B1, CYP2E1. The liver function biomarkers were greatly affected upon atrazine administration, and the serum levels of AST and ALT were significantly increased, while BWG%, albumin, globulins, and total proteins levels were markedly decreased. As a result of the above-mentioned influences of atrazine, histopathological changes in liver tissue were recorded in our findings. The administration of zinc oxide nanoparticles or vitamin C orally at a dose of 10 mg/kg and 200 mg/kg, respectively, for 30 days prior and along with atrazine, could significantly ameliorate the oxidative stress, inflammation, and apoptosis induced by atrazine and regulated the hepatic cytochrome P450 activities. Furthermore, they improved liver function biomarkers and histopathology. In conclusion, our results revealed that zinc oxide nanoparticles and vitamin C supplementations could effectively protect against atrazine-induced hepatotoxicity.
MeSH term(s)	Humans ; Rats ; Animals ; Zinc Oxide/pharmacology ; Atrazine/toxicity ; Atrazine/metabolism ; Reactive Oxygen Species/metabolism ; Ascorbic Acid/pharmacology ; Ascorbic Acid/metabolism ; Liver/metabolism ; Antioxidants/metabolism ; Oxidative Stress ; Apoptosis ; Vitamins/pharmacology ; Inflammation/chemically induced ; Inflammation/drug therapy ; Inflammation/metabolism ; Cytochrome P-450 Enzyme System/metabolism ; Biomarkers/metabolism ; Chemical and Drug Induced Liver Injury/drug therapy ; Chemical and Drug Induced Liver Injury/prevention & control ; Chemical and Drug Induced Liver Injury/metabolism ; Nanoparticles ; Immunomodulation
Chemical Substances	Zinc Oxide (SOI2LOH54Z) ; Atrazine (QJA9M5H4IM) ; Reactive Oxygen Species ; Ascorbic Acid (PQ6CK8PD0R) ; Antioxidants ; Vitamins ; Cytochrome P-450 Enzyme System (9035-51-2) ; Biomarkers
Language	English
Publishing date	2023-02-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	445336-0
ISSN	1559-0720 ; 0163-4984
ISSN (online)	1559-0720
ISSN	0163-4984
DOI	10.1007/s12011-023-03587-2
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