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  1. Article ; Online: Lessons learned from our life science tools startup journey.

    Molho, Joshua I / Gardner, Kelly / Herr, Amy E

    Analytica chimica acta

    2022  Volume 1244, Page(s) 340446

    Language English
    Publishing date 2022-10-31
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 1483436-4
    ISSN 1873-4324 ; 0003-2670
    ISSN (online) 1873-4324
    ISSN 0003-2670
    DOI 10.1016/j.aca.2022.340446
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Turn-induced isotachophoretic focusing in microfluidic channels

    Paschkewitz, John S / Molho, Joshua I / Xu, Hui / Bharadwaj, Rajiv / Park, C. Charles

    Electrophoresis. 2007 Dec., v. 28, no. 24

    2007  

    Abstract: Multifunctional microfluidic platforms that use ITP for sample stacking require the use of serpentine channels. When ITP is carried out in serpentine passages, the sample band shows dramatically different behavior than that observed in conventional on- ... ...

    Abstract Multifunctional microfluidic platforms that use ITP for sample stacking require the use of serpentine channels. When ITP is carried out in serpentine passages, the sample band shows dramatically different behavior than that observed in conventional on-chip CE. The band skew, defined as the distance between the leading and trailing edge of the band, is reduced and the sample is focused on the outside wall of the turn. This effect is explained using a simplified analytical model for the infinite Peclet number limit and the predicted trends are compared to full numerical simulations and experimental measurements. The outward focusing is driven by a wall-normal electric field that results from the skewed leading buffer-trailing buffer (LB-TB) interface. The results indicate that the degree of outward focusing is controlled by the ratio of the LB and TB conductivities, the ratio of the channel width to turn radius, and the Peclet number. Increasing the band skew, which shows a complex dependence on the nondimensional parameters, maximizes the outward focusing effect.
    Keywords electric field ; electrophoresis ; mathematical models ; serpentine
    Language English
    Dates of publication 2007-12
    Size p. 4561-4571.
    Publishing place Wiley-VCH Verlag
    Document type Article
    ZDB-ID 619001-7
    ISSN 1522-2683 ; 0173-0835
    ISSN (online) 1522-2683
    ISSN 0173-0835
    DOI 10.1002/elps.200700320
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 84/705: Show issues

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  3. Article: Turn-induced isotachophoretic focusing in microfluidic channels.

    Paschkewitz, John S / Molho, Joshua I / Xu, Hui / Bharadwaj, Rajiv / Park, C Charles

    Electrophoresis

    2007  Volume 28, Issue 24, Page(s) 4561–4571

    Abstract: Multifunctional microfluidic platforms that use ITP for sample stacking require the use of serpentine channels. When ITP is carried out in serpentine passages, the sample band shows dramatically different behavior than that observed in conventional on- ... ...

    Abstract Multifunctional microfluidic platforms that use ITP for sample stacking require the use of serpentine channels. When ITP is carried out in serpentine passages, the sample band shows dramatically different behavior than that observed in conventional on-chip CE. The band skew, defined as the distance between the leading and trailing edge of the band, is reduced and the sample is focused on the outside wall of the turn. This effect is explained using a simplified analytical model for the infinite Peclet number limit and the predicted trends are compared to full numerical simulations and experimental measurements. The outward focusing is driven by a wall-normal electric field that results from the skewed leading buffer-trailing buffer (LB-TB) interface. The results indicate that the degree of outward focusing is controlled by the ratio of the LB and TB conductivities, the ratio of the channel width to turn radius, and the Peclet number. Increasing the band skew, which shows a complex dependence on the nondimensional parameters, maximizes the outward focusing effect.
    MeSH term(s) Electrophoresis/methods ; Microfluidics/instrumentation
    Language English
    Publishing date 2007-12
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 619001-7
    ISSN 1522-2683 ; 0173-0835
    ISSN (online) 1522-2683
    ISSN 0173-0835
    DOI 10.1002/elps.200700320
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1868: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Preclinical Validation of the Utility of BLZ-100 in Providing Fluorescence Contrast for Imaging Spontaneous Solid Tumors.

    Fidel, Janean / Kennedy, Katie C / Dernell, William S / Hansen, Stacey / Wiss, Valorie / Stroud, Mark R / Molho, Joshua I / Knoblaugh, Sue E / Meganck, Jeffrey / Olson, James M / Rice, Brad / Parrish-Novak, Julia

    Cancer research

    2014  Volume 75, Issue 20, Page(s) 4283–4291

    Abstract: There is a need in surgical oncology for contrast agents that can enable real-time intraoperative visualization of solid tumors that can enable complete resections while sparing normal surrounding tissues. The Tumor Paint agent BLZ-100 is a peptide- ... ...

    Abstract There is a need in surgical oncology for contrast agents that can enable real-time intraoperative visualization of solid tumors that can enable complete resections while sparing normal surrounding tissues. The Tumor Paint agent BLZ-100 is a peptide-fluorophore conjugate that can specifically bind solid tumors and fluoresce in the near-infrared range, minimizing light scatter and signal attenuation. In this study, we provide a preclinical proof of concept for use of this imaging contrast agent as administered before surgery to dogs with a variety of naturally occurring spontaneous tumors. Imaging was performed on excised tissues as well as intraoperatively in a subset of cases. Actionable contrast was achieved between tumor tissue and surrounding normal tissues in adenocarcinomas, squamous cell carcinomas, mast cell tumors, and soft tissue sarcomas. Subcutaneous soft tissue sarcomas were labeled with the highest fluorescence intensity and greatest tumor-to-background signal ratio. Our results establish a foundation that rationalizes clinical studies in humans with soft tissue sarcoma, an indication with a notably high unmet need.
    MeSH term(s) Adolescent ; Animals ; Child ; Child, Preschool ; Contrast Media/administration & dosage ; Diagnostic Imaging/instrumentation ; Diagnostic Imaging/methods ; Disease Models, Animal ; Dogs ; Female ; Fluorescent Dyes/administration & dosage ; Humans ; Indocyanine Green/administration & dosage ; Indocyanine Green/analogs & derivatives ; Intraoperative Care ; Male ; Neoplasms/diagnosis ; Neoplasms/pathology ; Reproducibility of Results ; Scorpion Venoms/administration & dosage
    Chemical Substances Contrast Media ; Fluorescent Dyes ; Scorpion Venoms ; tozuleristide (835UH424TU) ; Indocyanine Green (IX6J1063HV)
    Language English
    Publishing date 2014-08-03
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 1432-1
    ISSN 1538-7445 ; 0008-5472
    ISSN (online) 1538-7445
    ISSN 0008-5472
    DOI 10.1158/0008-5472.CAN-15-0471
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uh III Zs.135/5: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: On-chip coupling of isoelectric focusing and free solution electrophoresis for multidimensional separations.

    Herr, Amy E / Molho, Joshua I / Drouvalakis, Katerina A / Mikkelsen, James C / Utz, Paul J / Santiago, Juan G / Kenny, Thomas W

    Analytical chemistry

    2002  Volume 75, Issue 5, Page(s) 1180–1187

    Abstract: We have developed an acrylic microfluidic device that sequentially couples liquid-phase isoelectric focusing (IEF) and free solution capillary electrophoresis (CE). Rapid separation (<1 min) and preconcentration (73x) of species were achieved in the ... ...

    Abstract We have developed an acrylic microfluidic device that sequentially couples liquid-phase isoelectric focusing (IEF) and free solution capillary electrophoresis (CE). Rapid separation (<1 min) and preconcentration (73x) of species were achieved in the initial IEF dimension. Using full-field fluorescence imaging, we observed nondispersive mobilization velocities on the order of 20 microm/s during characterization of the IEF step. This transport behavior allowed controlled electrokinetic mobilization of focused sample bands to a channel junction, where voltage switching was used to repeatedly inject effluent from the IEF dimension into an ampholyte-based CE separation. This second dimension was capable of analyzing all fluid volumes of interest from the IEF dimension, as IEF was 'parked' during each CE analysis and refocused prior to additional CE analyses. Investigation of each dimension of the integrated system showed time-dependent species displacement and band-broadening behavior consistent with IEF and CE, respectively. The peak capacity of the 2D system was approximately 1300. A comprehensive 2D analysis of a fluid volume spanning 15% of the total IEF channel length was completed in less than 5 min.
    MeSH term(s) Algorithms ; Diagnostic Imaging ; Electrophoresis/instrumentation ; Indicators and Reagents ; Isoelectric Focusing/instrumentation ; Microcomputers
    Chemical Substances Indicators and Reagents
    Language English
    Publishing date 2002-11-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/ac026239a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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