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Article ; Online: GDF5 deficiency prevents cardiac rupture following acute myocardial infarction in mice.

Shikatani, Eric A / Wang, Tao / Dingwell, Luke S / White-Dzuro, Colin / Momen, Abdul / Husain, Mansoor

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology

2023 Volume 68, Page(s) 107581

Abstract: Background: We previously showed that growth differentiation factor 5 (GDF5) limits infarct expansion post-myocardial infarction (MI). We now examine the acute post-MI role of GDF5 in cardiac rupture.: Methods and results: Following permanent ... ...

Abstract	Background: We previously showed that growth differentiation factor 5 (GDF5) limits infarct expansion post-myocardial infarction (MI). We now examine the acute post-MI role of GDF5 in cardiac rupture. Methods and results: Following permanent ligation of the left anterior descending artery, GDF5 deficiency (i.e., GDF5 knockout mice) reduced the incidence of cardiac rupture (4/24 vs. 17/24; P < .05), and improved survival over 28-d compared to wild-type (WT) mice (79% vs. 25%; P < .0001). Moreover, at 3-d post-MI, GDF5-deficient mice manifest: (a) reduced heart weight/body weight ratio (P < .0001) without differences in infarct size or cardiomyocyte size; (b) increased infarct zone expression of Col1a1 (P < .05) and Col3a1 (P < .01), suggesting increased myocardial fibrosis; and (c) reduced aortic and left ventricular peak systolic pressures (P ≤ .05), suggesting reduced afterload. Despite dysregulated inflammatory markers and reduced circulating monocytes in GDF5-deficient mice at 3-d post-MI, reciprocal bone marrow transplantation (BMT) failed to implicate GDF5 in BM-derived cells, suggesting the involvement of tissue-resident GDF5 expression in cardiac rupture. Conclusions: Loss of GDF5 reduces cardiac rupture post-MI with increased myocardial fibrosis and lower afterload, albeit at the cost of chronic adverse remodeling.
MeSH term(s)	Animals ; Mice ; Disease Models, Animal ; Fibrosis ; Growth Differentiation Factor 5/genetics ; Growth Differentiation Factor 5/metabolism ; Heart Rupture/genetics ; Heart Rupture/metabolism ; Mice, Inbred C57BL ; Mice, Knockout ; Myocardial Infarction/complications ; Myocardial Infarction/genetics ; Myocardial Infarction/metabolism ; Myocardium/pathology
Chemical Substances	Growth Differentiation Factor 5
Language	English
Publishing date	2023-10-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	1134600-0
ISSN	1879-1336 ; 1054-8807
ISSN (online)	1879-1336
ISSN	1054-8807
DOI	10.1016/j.carpath.2023.107581
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Article: Draft-genome analysis provides insights into the virulence properties and genome plasticity of Vibrio fluvialis organisms isolated from shrimp farms and Turag river in Bangladesh

Hossain, Maqsud / Ibne Momen, Abdul Mueed / Rahman, Aura / Biswas, Juthi / Yasmin, Mahmuda / Nessa, Jamalun / Ahsan, Chowdhury Rafiqul

Archives of microbiology. 2022 Aug., v. 204, no. 8

2022

Abstract: Vibrio fluvialis is an opportunistic waterborne and seafood-borne enteric pathogen capable of causing severe diarrhea leading to death. This pathogen is endemic to Bangladesh, a country which is a major producer of cultured shrimp and wild-caught prawns. ...

Abstract	Vibrio fluvialis is an opportunistic waterborne and seafood-borne enteric pathogen capable of causing severe diarrhea leading to death. This pathogen is endemic to Bangladesh, a country which is a major producer of cultured shrimp and wild-caught prawns. In this study, we carried out whole-genome sequencing of three V. fluvialis organisms isolated from shrimp farm and river sediment showing strong pathogenic characteristics in vivo and in vitro and compared their genomes against other V. fluvialis and related pathogenic species to glean insights into their potential as pathogens. Numerous virulence-associated genes including hemolysins, cytolysins, three separate Type IV pili, Types II and VI secretion systems, biofilm, and the V. cholerae pathogenesis regulating gene, toxR, were identified. Moreover, we found strain S-10 to have the propensity to acquire antibiotic resistance genes through horizontal gene transfer. These findings indicate that shrimp farms and rivers could be potential sources of V. fluvialis organisms which are an infection threat of public health concern.
Keywords	Vibrio fluvialis ; biofilm ; death ; diarrhea ; enteropathogens ; farms ; fimbriae ; hemolysins ; horizontal gene transfer ; microbiology ; pathogenesis ; public health ; rivers ; secretion ; sediments ; shrimp ; virulence ; Bangladesh
Language	English
Dates of publication	2022-08
Size	p. 527.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	124824-8
ISSN	1432-072X ; 0302-8933
ISSN (online)	1432-072X
ISSN	0302-8933
DOI	10.1007/s00203-022-03128-w
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Article ; Online: Impact of ovary-intact menopause in a mouse model of heart failure with preserved ejection fraction.

Troy, Aaron M / Normukhamedova, Diyora / Grothe, Daniela / Momen, Abdul / Zhou, Yu-Qing / McFadden, Meghan / Hussain, Mansoor / Billia, Filio / Cheng, Hai-Ling Margaret

American journal of physiology. Heart and circulatory physiology

2024 Volume 326, Issue 3, Page(s) H522–H537

Abstract: Heart failure with preserved ejection fraction (HFpEF) afflicts over half of all patients with heart failure and is a debilitating and fatal syndrome affecting postmenopausal women more than any other demographic. This bias toward older females calls ... ...

Abstract	Heart failure with preserved ejection fraction (HFpEF) afflicts over half of all patients with heart failure and is a debilitating and fatal syndrome affecting postmenopausal women more than any other demographic. This bias toward older females calls into question the significance of menopause in the development of HFpEF, but this question has not been probed in detail. In this study, we report the first investigation into the impact of ovary-intact menopause in the context of HFpEF. To replicate the human condition as faithfully as possible, vinylcyclohexene dioxide (VCD) was used to accelerate ovarian failure (AOF) in female mice while leaving the ovaries intact. HFpEF was established with a mouse model that involves two stressors typical in humans: a high-fat diet and hypertension induced from the nitric oxide synthase inhibitor
MeSH term(s)	Humans ; Middle Aged ; Female ; Mice ; Animals ; Heart Failure/diagnostic imaging ; Heart Failure/etiology ; Heart Failure/pathology ; Ovary/pathology ; Stroke Volume/physiology ; Heart Diseases ; Cardiomyopathies ; Menopause
Language	English
Publishing date	2024-01-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	603838-4
ISSN	1522-1539 ; 0363-6135
ISSN (online)	1522-1539
ISSN	0363-6135
DOI	10.1152/ajpheart.00733.2023
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Article ; Online: Draft-genome analysis provides insights into the virulence properties and genome plasticity of Vibrio fluvialis organisms isolated from shrimp farms and Turag river in Bangladesh.

Hossain, Maqsud / Ibne Momen, Abdul Mueed / Rahman, Aura / Biswas, Juthi / Yasmin, Mahmuda / Nessa, Jamalun / Ahsan, Chowdhury Rafiqul

Archives of microbiology

2022 Volume 204, Issue 8, Page(s) 527

Abstract	Vibrio fluvialis is an opportunistic waterborne and seafood-borne enteric pathogen capable of causing severe diarrhea leading to death. This pathogen is endemic to Bangladesh, a country which is a major producer of cultured shrimp and wild-caught prawns. In this study, we carried out whole-genome sequencing of three V. fluvialis organisms isolated from shrimp farm and river sediment showing strong pathogenic characteristics in vivo and in vitro and compared their genomes against other V. fluvialis and related pathogenic species to glean insights into their potential as pathogens. Numerous virulence-associated genes including hemolysins, cytolysins, three separate Type IV pili, Types II and VI secretion systems, biofilm, and the V. cholerae pathogenesis regulating gene, toxR, were identified. Moreover, we found strain S-10 to have the propensity to acquire antibiotic resistance genes through horizontal gene transfer. These findings indicate that shrimp farms and rivers could be potential sources of V. fluvialis organisms which are an infection threat of public health concern.
MeSH term(s)	Aquaculture ; Bangladesh ; Rivers ; Seafood ; Vibrio/genetics ; Vibrio cholerae ; Virulence/genetics
Language	English
Publishing date	2022-07-27
Publishing country	Germany
Document type	Journal Article
ZDB-ID	124824-8
ISSN	1432-072X ; 0302-8933
ISSN (online)	1432-072X
ISSN	0302-8933
DOI	10.1007/s00203-022-03128-w
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Article ; Online: Microbial energy metabolism fuels an intestinal macrophage niche in solitary isolated lymphoid tissues through purinergic signaling.

Science immunology

2023 Volume 8, Issue 86, Page(s) eabq4573

Abstract: Maintaining macrophage (MΦ) heterogeneity is critical to ensure intestinal tissue homeostasis and host defense. The gut microbiota and host factors are thought to synergistically guide intestinal MΦ development, although the exact nature, regulation, and ...

Abstract	Maintaining macrophage (MΦ) heterogeneity is critical to ensure intestinal tissue homeostasis and host defense. The gut microbiota and host factors are thought to synergistically guide intestinal MΦ development, although the exact nature, regulation, and location of such collaboration remain unclear. Here, we report that microbial biochemical energy metabolism promotes colony-stimulating factor 2 (CSF2) production by group 3 innate lymphoid cells (ILC3s) within solitary isolated lymphoid tissues (SILTs) in a cell-extrinsic, NLRP3/P2X7R-dependent fashion in the steady state. Tissue-infiltrating monocytes accumulating around SILTs followed a spatially constrained, distinct developmental trajectory into SILT-associated MΦs (SAMs). CSF2 regulated the mitochondrial membrane potential and reactive oxygen species production of SAMs and contributed to the antimicrobial defense against enteric bacterial infections. Collectively, these findings identify SILTs and CSF2-producing ILC3s as a microanatomic niche for intestinal MΦ development and functional programming fueled by the integration of commensal microbial energy metabolism.
MeSH term(s)	Immunity, Innate ; Lymphocytes/metabolism ; Intestines ; Lymphoid Tissue ; Macrophages
Language	English
Publishing date	2023-08-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2470-9468
ISSN (online)	2470-9468
DOI	10.1126/sciimmunol.abq4573
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Article: Factors affecting the attractiveness of medical tourism destination: an empirical study on India- review article.

Sultana, Seyama / Haque, Ahasanul / Momen, Abdul / Yasmin, Farzana

Iranian journal of public health

2014 Volume 43, Issue 7, Page(s) 867–876

Abstract: Background: In this edge, medical tourism is not a new idea. Medical treatment is one of the essential demands of human beings and it requires high quality and intensive care. Beside western world, few developing countries are playing key roles as ... ...

Abstract	Background: In this edge, medical tourism is not a new idea. Medical treatment is one of the essential demands of human beings and it requires high quality and intensive care. Beside western world, few developing countries are playing key roles as medical tourism destinations. India is one of the leading names among these countries. The purpose of the paper is to find the factors influencing the attractiveness of India as a health tourism destination. Methods: The study has found the major contributing factors and their relative importance in the attractiveness of the health tourism destination that is India from consumers' perspectives by conducting survey with an application of structural equation modelling approach. Results: In Indian context, medical tourists consider service quality and cost mostly to select any medical destination. In addition they also give value to the destination competitiveness but tourist attitude is less important in comparison with other factors affecting their destination choice. Since the study has used structural equation modelling approach to test the hypothesis and figure out the relative importance of the factors, the fundamental indices such as Normed Chi square(less than 3), RMSEA (less than 0.08) and CFI (more than 0.90) values show the overall model fit of the proposed model. Conclusion: In order to transform a country such as India as an attractive and competitive medical tourist destination in this time of globalization, a step should be taken to control cost ensuring the quality of services.
Language	English
Publishing date	2014-07
Publishing country	Iran
Document type	Journal Article ; Review
ISSN	2251-6085
ISSN	2251-6085
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Article ; Online: Difficulties to enter the left ventricle during percutaneous transvenous mitral commissurotomy (PTMC)- our experiences of 80 cases with modified techniques.

Momen, Abdul / Ali, Md Zulfikar / Hyder Chowdhury, Naharuma Aive / Huda, Reaz Mahmud / Nobi, Abm Nurun / Rahman, Ashraf Ur / Alam, Iftequar / Sayami, Lima Asrin / Alam, Md Abul / Hossain, Md Delwar / Tasneem, Samia

Indian heart journal

2021 Volume 73, Issue 5, Page(s) 612–616

Abstract: Background: Percutaneous transvenous mitral commissurotomy (PTMC) is the standard of treatment for symptomatic severe rheumatic mitral stenosis (MS). PTMC has the standard Inoue technique, but we have to modify the procedure in many technically ... ...

Abstract	Background: Percutaneous transvenous mitral commissurotomy (PTMC) is the standard of treatment for symptomatic severe rheumatic mitral stenosis (MS). PTMC has the standard Inoue technique, but we have to modify the procedure in many technically challenging cases, especially to cross the mitral valve. Methodology: Two over-the-wire strategies to enter the LV were taken in 80 complex cases of PTMC. The first one was done by exchanging the J-shaped wire from the balloon, introducing the spring wire into it, and pushing it into LV. The second one-removal of balloon keeping the spring wire in LA and the Mullin's sheath was introduced, and the tip of the wire was pushed into LV, and the balloon was introduced over the wire. We also changed the left atrium (LA) graphy in the RAO view instead of the AP view to facilitating entry into LV. Results: We succeeded in 76 (95 %) cases. Strategy one was applied to all but successful in only 25 cases (31 %), and strategy 2 was applied in the remaining. Strategy 1 required less procedural time and fluoroscopic time in comparison to strategy 2 (40 ± 10 vs 60 ± 16 min, 25 ± 7 vs 35 ± 8 min). After modification of taking the LA graphy in RAO view, our rate of facing the difficulties decreased from 21 % to 9 %. Critical MS (31 %) and the giant LA (30 %) were the topmost causes of difficulties. No major complications were recorded. Conclusion: Over-the-wire entry into LV is cost-effective, requiring no new equipment and is safe and can be used in complex cases.
MeSH term(s)	Catheterization ; Heart Atria ; Heart Ventricles/diagnostic imaging ; Heart Ventricles/surgery ; Humans ; Mitral Valve/diagnostic imaging ; Mitral Valve/surgery ; Mitral Valve Stenosis/diagnosis ; Mitral Valve Stenosis/surgery
Language	English
Publishing date	2021-07-24
Publishing country	India
Document type	Journal Article
ZDB-ID	604366-5
ISSN	2213-3763 ; 0019-4832
ISSN (online)	2213-3763
ISSN	0019-4832
DOI	10.1016/j.ihj.2021.07.002
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Article ; Online: Selective loss of resident macrophage-derived insulin-like growth factor-1 abolishes adaptive cardiac growth to stress.

Zaman, Rysa / Hamidzada, Homaira / Kantores, Crystal / Wong, Anthony / Dick, Sarah A / Wang, Yiming / Momen, Abdul / Aronoff, Laura / Lin, Julia / Razani, Babak / Mital, Seema / Billia, Filio / Lavine, Kory J / Nejat, Sara / Epelman, Slava

Immunity

2021 Volume 54, Issue 9, Page(s) 2057–2071.e6

Abstract: Hypertension affects one-third of the world's population, leading to cardiac dysfunction that is modulated by resident and recruited immune cells. Cardiomyocyte growth and increased cardiac mass are essential to withstand hypertensive stress; however, ... ...

Abstract	Hypertension affects one-third of the world's population, leading to cardiac dysfunction that is modulated by resident and recruited immune cells. Cardiomyocyte growth and increased cardiac mass are essential to withstand hypertensive stress; however, whether immune cells are involved in this compensatory cardioprotective process is unclear. In normotensive animals, single-cell transcriptomics of fate-mapped self-renewing cardiac resident macrophages (RMs) revealed transcriptionally diverse cell states with a core repertoire of reparative gene programs, including high expression of insulin-like growth factor-1 (Igf1). Hypertension drove selective in situ proliferation and transcriptional activation of some cardiac RM states, directly correlating with increased cardiomyocyte growth. During hypertension, inducible ablation of RMs or selective deletion of RM-derived Igf1 prevented adaptive cardiomyocyte growth, and cardiac mass failed to increase, which led to cardiac dysfunction. Single-cell transcriptomics identified a conserved IGF1-expressing macrophage subpopulation in human cardiomyopathy. Here we defined the absolute requirement of RM-produced IGF-1 in cardiac adaptation to hypertension.
MeSH term(s)	Adaptation, Physiological/physiology ; Animals ; Heart Failure/etiology ; Heart Failure/metabolism ; Heart Failure/pathology ; Humans ; Hypertension/complications ; Hypertension/immunology ; Hypertension/metabolism ; Infant ; Insulin-Like Growth Factor I/metabolism ; Macrophages/metabolism ; Male ; Mice ; Middle Aged ; Myocardium/immunology ; Myocardium/metabolism ; Myocardium/pathology ; Ventricular Remodeling/physiology
Chemical Substances	Insulin-Like Growth Factor I (67763-96-6)
Language	English
Publishing date	2021-08-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1217235-2
ISSN	1097-4180 ; 1074-7613
ISSN (online)	1097-4180
ISSN	1074-7613
DOI	10.1016/j.immuni.2021.07.006
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Article ; Online: Influence of ABO blood group on von Willebrand factor tests in healthy Saudi blood donors.

Alharbi, Abdullah / Hassan, Salwa Bakr / Al-Momen, Abdul-Kareem / Al-Saleh, Khalid / Nasr, Rasheed / Kohgear, Haitham / Owaidah, Tarek

Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis

2018 Volume 29, Issue 2, Page(s) 211–215

Abstract: Von Willebrand disease is a common bleeding disorder. The wide variation in von Willebrand factor (VWF) levels between and within normal individuals highlights the clinical challenge of defining its cutoff value. Although studies on the influence of ... ...

Abstract	: Von Willebrand disease is a common bleeding disorder. The wide variation in von Willebrand factor (VWF) levels between and within normal individuals highlights the clinical challenge of defining its cutoff value. Although studies on the influence of ethnicity on ABO phenotypes and the levels of VWF have been carried out on different ethnicities, there is a lack of such data among Arab population. We aimed to evaluate the correlation of ABO phenotypes with all the parameters of the minimal test panel of VWF including VWF antigen, VWF activity using the ristocetin cofactor and the collagen binding activity assays, and factor VIII coagulant activity (VWF:Ag, VWF:RCo, VWF:CB and FVIII:C) tested in a normal Arab population, and to estimate ABO-specific normal reference range. Blood samples were collected from 87 healthy donors in Riyadh to determine levels of factor VIII and VWF panel between the various ABO phenotypes. The highest mean values of factor VIII : C (128 U/dl), VWF : Ag (125 U/dl), VWF : RCo (109 U/dl) and VWF : CB (91 U/dl) were observed with type AB and the lowest mean values of factor VIII : C (81 U/dl), VWF : Ag (85 U/dl), VWF : RCo (73 U/dl) and VWF : CB (70 U/dl) corresponded to type O. ABO phenotypes significantly influence plasma levels of VWF parameters in Arab nations as seen with other ethnicity. Hence, ABO-specific normal ranges of the minimal test panel of VWF and factor VIII : C are essential for the appropriate prediction of mild von Willebrand disease. Further study including a larger categorized sample size is required to generalize the test panel on the Arab population.
MeSH term(s)	ABO Blood-Group System/metabolism ; Adolescent ; Adult ; Aged ; Blood Donors ; Female ; Humans ; Male ; Middle Aged ; Saudi Arabia ; Young Adult ; von Willebrand Factor/metabolism
Chemical Substances	ABO Blood-Group System ; von Willebrand Factor
Language	English
Publishing date	2018-03
Publishing country	England
Document type	Journal Article
ZDB-ID	1033551-1
ISSN	1473-5733 ; 0957-5235
ISSN (online)	1473-5733
ISSN	0957-5235
DOI	10.1097/MBC.0000000000000709
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Article ; Online: Cardioprotective GLP-1 metabolite prevents ischemic cardiac injury by inhibiting mitochondrial trifunctional protein-α.

Siraj, M Ahsan / Mundil, Dhanwantee / Beca, Sanja / Momen, Abdul / Shikatani, Eric A / Afroze, Talat / Sun, Xuetao / Liu, Ying / Ghaffari, Siavash / Lee, Warren / Wheeler, Michael B / Keller, Gordon / Backx, Peter / Husain, Mansoor

The Journal of clinical investigation

2020 Volume 130, Issue 3, Page(s) 1392–1404

Abstract: Mechanisms mediating the cardioprotective actions of glucagon-like peptide 1 (GLP-1) were unknown. Here, we show in both ex vivo and in vivo models of ischemic injury that treatment with GLP-1(28-36), a neutral endopeptidase-generated (NEP-generated) ... ...

Abstract	Mechanisms mediating the cardioprotective actions of glucagon-like peptide 1 (GLP-1) were unknown. Here, we show in both ex vivo and in vivo models of ischemic injury that treatment with GLP-1(28-36), a neutral endopeptidase-generated (NEP-generated) metabolite of GLP-1, was as cardioprotective as GLP-1 and was abolished by scrambling its amino acid sequence. GLP-1(28-36) enters human coronary artery endothelial cells (caECs) through macropinocytosis and acts directly on mouse and human coronary artery smooth muscle cells (caSMCs) and caECs, resulting in soluble adenylyl cyclase Adcy10-dependent (sAC-dependent) increases in cAMP, activation of protein kinase A, and cytoprotection from oxidative injury. GLP-1(28-36) modulates sAC by increasing intracellular ATP levels, with accompanying cAMP accumulation lost in sAC-/- cells. We identify mitochondrial trifunctional protein-α (MTPα) as a binding partner of GLP-1(28-36) and demonstrate that the ability of GLP-1(28-36) to shift substrate utilization from oxygen-consuming fatty acid metabolism toward oxygen-sparing glycolysis and glucose oxidation and to increase cAMP levels is dependent on MTPα. NEP inhibition with sacubitril blunted the ability of GLP-1 to increase cAMP levels in coronary vascular cells in vitro. GLP-1(28-36) is a small peptide that targets novel molecular (MTPα and sAC) and cellular (caSMC and caEC) mechanisms in myocardial ischemic injury.
MeSH term(s)	Animals ; Cardiotonic Agents/metabolism ; Coronary Vessels/metabolism ; Coronary Vessels/pathology ; Cyclic AMP/genetics ; Cyclic AMP/metabolism ; Cyclic AMP-Dependent Protein Kinases/genetics ; Cyclic AMP-Dependent Protein Kinases/metabolism ; Endothelial Cells/metabolism ; Endothelial Cells/pathology ; Glucagon-Like Peptide 1/genetics ; Glucagon-Like Peptide 1/metabolism ; Humans ; Mice ; Mice, Knockout ; Mitochondria, Heart/genetics ; Mitochondria, Heart/metabolism ; Mitochondria, Heart/pathology ; Mitochondrial Trifunctional Protein, alpha Subunit/genetics ; Mitochondrial Trifunctional Protein, alpha Subunit/metabolism ; Muscle, Smooth, Vascular/metabolism ; Muscle, Smooth, Vascular/pathology ; Myocardial Ischemia/genetics ; Myocardial Ischemia/metabolism ; Myocardial Ischemia/pathology ; Myocardial Ischemia/prevention & control ; Myocytes, Smooth Muscle/metabolism ; Myocytes, Smooth Muscle/pathology ; Second Messenger Systems/genetics
Chemical Substances	Cardiotonic Agents ; Glucagon-Like Peptide 1 (89750-14-1) ; Cyclic AMP (E0399OZS9N) ; Mitochondrial Trifunctional Protein, alpha Subunit (EC 1.1.1.211) ; Cyclic AMP-Dependent Protein Kinases (EC 2.7.11.11)
Language	English
Publishing date	2020-01-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3067-3
ISSN	1558-8238 ; 0021-9738
ISSN (online)	1558-8238
ISSN	0021-9738
DOI	10.1172/JCI99934
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