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  1. Article ; Online: Simulated Performance of Electroenzymatic Glutamate Biosensors In Vivo Illuminates the Complex Connection to Calibration In Vitro.

    Clay, Mackenzie / Monbouquette, Harold G

    ACS chemical neuroscience

    2021  Volume 12, Issue 22, Page(s) 4275–4285

    Abstract: Detailed simulations show that the relationship between electroenzymatic glutamate (Glut) sensor performance in vitro and that modeled in vivo is complicated by the influence of both resistances to mass transfer and clearance rates of Glut and ... ...

    Abstract Detailed simulations show that the relationship between electroenzymatic glutamate (Glut) sensor performance in vitro and that modeled in vivo is complicated by the influence of both resistances to mass transfer and clearance rates of Glut and H
    MeSH term(s) Biosensing Techniques ; Calibration ; Glutamic Acid ; Hydrogen Peroxide ; Models, Theoretical
    Chemical Substances Glutamic Acid (3KX376GY7L) ; Hydrogen Peroxide (BBX060AN9V)
    Language English
    Publishing date 2021-11-04
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1948-7193
    ISSN (online) 1948-7193
    DOI 10.1021/acschemneuro.1c00365
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Microcontact printing of choline oxidase using a polycation-functionalized zwitterionic polymer as enzyme immobilization matrix.

    Zhao, Ming / Cao, Yan / Huang, I-Wen / Monbouquette, Harold G

    The Analyst

    2023  Volume 148, Issue 23, Page(s) 5949–5956

    Abstract: Highly sensitive and selective choline microbiosensors were constructed by microcontact printing (μCP) of choline oxidase (ChOx) in a crosslinked, polyamine-functionalized zwitterionic polymer matrix on microelectrode arrays (MEAs). μCP has emerged as a ... ...

    Abstract Highly sensitive and selective choline microbiosensors were constructed by microcontact printing (μCP) of choline oxidase (ChOx) in a crosslinked, polyamine-functionalized zwitterionic polymer matrix on microelectrode arrays (MEAs). μCP has emerged as a potential means to create implantable, multiplexed sensor microprobes, which requires the targeted deposition of different sensor materials to specific microelectrode sites on a MEA. However, the less than sufficient enzyme loading and inadequate spatial resolution achieved with current μCP approaches has limited adoption of the method for electroenzymatic microsensors. A novel polymer, poly(2-methacryloyloxyethyl phosphorylcholine)-
    MeSH term(s) Animals ; Polymers ; Alcohol Oxidoreductases ; Choline ; Mammals
    Chemical Substances polycations ; Polymers ; choline oxidase (EC 1.1.3.17) ; Alcohol Oxidoreductases (EC 1.1.-) ; Choline (N91BDP6H0X)
    Language English
    Publishing date 2023-11-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d3an01263h
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  3. Article: Nucleic acid amplification-free detection of DNA and RNA at ultralow concentration

    Cao, Yan / Zheng, Zhenrong / Monbouquette, Harold G

    Current opinion in biotechnology. 2021 Oct., v. 71

    2021  

    Abstract: The broad spectrum of approaches for nucleic acid amplification-free detection of DNA and RNA at single-digit attomolar (10⁻¹⁸ M) concentration and lower is reviewed. These low concentrations correspond roughly to the most clinically desirable detection ... ...

    Abstract The broad spectrum of approaches for nucleic acid amplification-free detection of DNA and RNA at single-digit attomolar (10⁻¹⁸ M) concentration and lower is reviewed. These low concentrations correspond roughly to the most clinically desirable detection range for pathogen-specific nucleic acid as well as the detection limits of commercially available, nucleic acid amplification tests based primarily on polymerase chain reaction (PCR). The need for more rapid and inexpensive, yet still highly accurate tests, has become evident during the pandemic. It is expected that publication of reports describing improved tests will accelerate soon, and this review covers the wide variety of detection methods based on both optical and electrical measurements that have been conceived over recent years, enabled generally by the advent of nanotechnology.
    Keywords DNA ; RNA ; biotechnology ; gene amplification ; pandemic ; polymerase chain reaction
    Language English
    Dates of publication 2021-10
    Size p. 145-150.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2021.07.022
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  4. Article ; Online: Correction: An amplification-free, 16S rRNA test for

    Zheng, Zhenrong / Cao, Yan / Chandrasekaran, Sukantha / Schmidt, Jacob J / Garner, Omai B / Monbouquette, Harold G

    Sensors & diagnostics

    2023  Volume 2, Issue 2, Page(s) 468

    Abstract: This corrects the article DOI: 10.1039/D2SD00128D.]. ...

    Abstract [This corrects the article DOI: 10.1039/D2SD00128D.].
    Language English
    Publishing date 2023-01-30
    Publishing country England
    Document type Published Erratum
    ISSN 2635-0998
    ISSN (online) 2635-0998
    DOI 10.1039/d3sd90005c
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  5. Article ; Online: Nucleic acid amplification-free detection of DNA and RNA at ultralow concentration.

    Cao, Yan / Zheng, Zhenrong / Monbouquette, Harold G

    Current opinion in biotechnology

    2021  Volume 71, Page(s) 145–150

    Abstract: The broad spectrum of approaches for nucleic acid amplification-free detection of DNA and RNA at single-digit attomolar ( ... ...

    Abstract The broad spectrum of approaches for nucleic acid amplification-free detection of DNA and RNA at single-digit attomolar (10
    MeSH term(s) DNA/genetics ; Nucleic Acid Amplification Techniques ; Nucleic Acids ; Polymerase Chain Reaction ; RNA/genetics
    Chemical Substances Nucleic Acids ; RNA (63231-63-0) ; DNA (9007-49-2)
    Language English
    Publishing date 2021-08-07
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2021.07.022
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  6. Article ; Online: Flexible and Implantable Polyimide Aptamer-Field-Effect Transistor Biosensors.

    Zhao, Chuanzhen / Man, Tianxing / Cao, Yan / Weiss, Paul S / Monbouquette, Harold G / Andrews, Anne M

    ACS sensors

    2022  Volume 7, Issue 12, Page(s) 3644–3653

    Abstract: Monitoring neurochemical signaling across time scales is critical to understanding how brains encode and store information. Flexible (vs stiff) devices have been shown to ... ...

    Abstract Monitoring neurochemical signaling across time scales is critical to understanding how brains encode and store information. Flexible (vs stiff) devices have been shown to improve
    MeSH term(s) Serotonin ; Biosensing Techniques ; Semiconductors ; Aptamers, Nucleotide/chemistry
    Chemical Substances Serotonin (333DO1RDJY) ; Aptamers, Nucleotide
    Language English
    Publishing date 2022-11-18
    Publishing country United States
    Document type Research Support, N.I.H., Extramural ; Letter
    ISSN 2379-3694
    ISSN (online) 2379-3694
    DOI 10.1021/acssensors.2c01909
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  7. Article ; Online: An amplification-free, 16S rRNA test for

    Zheng, Zhenrong / Cao, Yan / Chandrasekaran, Sukantha / Schmidt, Jacob J / Garner, Omai B / Monbouquette, Harold G

    Sensors & diagnostics

    2022  Volume 2, Issue 1, Page(s) 163–167

    Abstract: An amplification-free, nanopore-based nucleic acid detection platform has been demonstrated for rapid, 16S rRNA sequence-specific detection ... ...

    Abstract An amplification-free, nanopore-based nucleic acid detection platform has been demonstrated for rapid, 16S rRNA sequence-specific detection of
    Language English
    Publishing date 2022-11-11
    Publishing country England
    Document type Journal Article
    ISSN 2635-0998
    ISSN (online) 2635-0998
    DOI 10.1039/d2sd00128d
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: A Detailed Model of Electroenzymatic Glutamate Biosensors To Aid in Sensor Optimization and in Applications in Vivo.

    Clay, Mackenzie / Monbouquette, Harold G

    ACS chemical neuroscience

    2017  Volume 9, Issue 2, Page(s) 241–251

    Abstract: Simulations conducted with a detailed model of glutamate biosensor performance describe the observed sensor performance well, illustrate the limits of sensor performance, and suggest a path toward sensor optimization. Glutamate is the most important ... ...

    Abstract Simulations conducted with a detailed model of glutamate biosensor performance describe the observed sensor performance well, illustrate the limits of sensor performance, and suggest a path toward sensor optimization. Glutamate is the most important excitatory neurotransmitter in the brain, and electroenzymatic sensors have emerged as a useful tool for the monitoring of glutamate signaling in vivo. However, the utility of these sensors currently is limited by their sensitivity and response time. A mathematical model of a typical glutamate biosensor consisting of a Pt electrode coated with a permselective polymer film and a top layer of cross-linked glutamate oxidase has been constructed in terms of differential material balances on glutamate, H
    MeSH term(s) Biosensing Techniques/instrumentation ; Computer Simulation ; Enzymes, Immobilized/chemistry ; Equipment Design ; Glutamic Acid/chemistry ; Hydrogen Peroxide/chemistry ; Kinetics ; Models, Theoretical ; Oxidoreductases/chemistry ; Oxygen/chemistry ; Polymers/chemistry
    Chemical Substances Enzymes, Immobilized ; Polymers ; Glutamic Acid (3KX376GY7L) ; Hydrogen Peroxide (BBX060AN9V) ; Oxidoreductases (EC 1.-) ; Oxygen (S88TT14065)
    Language English
    Publishing date 2017-11-10
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1948-7193
    ISSN (online) 1948-7193
    DOI 10.1021/acschemneuro.7b00262
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  9. Article ; Online: Electroenzymatic choline sensing at near the theoretical performance limit.

    Huang, I-Wen / Clay, Mackenzie / Cao, Yan / Nie, Jingjing / Guo, Yuwan / Monbouquette, Harold G

    The Analyst

    2020  Volume 146, Issue 3, Page(s) 1040–1047

    Abstract: A high performance, electroenzymatic microsensor for choline based on choline oxidase (ChOx) immobilized on Pt coated with permselective polymer layers has been created that exhibits sensitivity approaching the theoretical performance limit. Sensor ... ...

    Abstract A high performance, electroenzymatic microsensor for choline based on choline oxidase (ChOx) immobilized on Pt coated with permselective polymer layers has been created that exhibits sensitivity approaching the theoretical performance limit. Sensor construction was guided by simulations performed with a detailed mathematical model. Implantable microsensors with an array of electroenzymatic sensing sites provide a means to record concentration changes of choline, an effective surrogate for acetylcholine due to its very rapid turnover in the brain, and other neurochemicals in vivo. However, electroenzymatic sensors generally have insufficient sensitivity and response time to monitor neurotransmitter signaling on the millisecond timescale with cellular-level spatial resolution. Model simulations suggested that choline sensor performance can be improved significantly by optimizing immobilized ChOx layer thickness and minimizing the thicknesses of permselective polymer coatings as well. Electroenzymatic choline sensors constructed with a ∼5 μm-thick crosslinked ChOx layer atop 200 nm-thick permselective films (poly(m-phenylenediamine) and Nafion) exhibited unprecedented sensitivity and response time of 660 ± 40 nA μM
    MeSH term(s) Acetylcholine ; Biosensing Techniques ; Choline ; Microelectrodes ; Polymers
    Chemical Substances Polymers ; Choline (N91BDP6H0X) ; Acetylcholine (N9YNS0M02X)
    Language English
    Publishing date 2020-12-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d0an01939a
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  10. Article ; Online: Electroenzymatic glutamate sensing at near the theoretical performance limit.

    Huang, I-Wen / Clay, Mackenzie / Wang, Siqi / Guo, Yuwan / Nie, Jingjing / Monbouquette, Harold G

    The Analyst

    2020  Volume 145, Issue 7, Page(s) 2602–2611

    Abstract: The sensitivity and response time of glutamate sensors based on glutamate oxidase immobilized on planar platinum microelectrodes have been improved to near the theoretical performance limits predicted by a detailed mathematical model. Microprobes with an ...

    Abstract The sensitivity and response time of glutamate sensors based on glutamate oxidase immobilized on planar platinum microelectrodes have been improved to near the theoretical performance limits predicted by a detailed mathematical model. Microprobes with an array of electroenzymatic sensing sites have emerged as useful tools for the monitoring of glutamate and other neurotransmitters in vivo; and implemented as such, they can be used to study many complex neurological diseases and disorders including Parkinson's disease and drug addiction. However, less than optimal sensitivity and response time has limited the spatiotemporal resolution of these promising research tools. A mathematical model has guided systematic improvement of an electroenzymatic glutamate microsensor constructed with a 1-2 μm-thick crosslinked glutamate oxidase layer and underlying permselective coating of polyphenylenediamine and Nafion reduced to less than 200 nm thick. These design modifications led to a nearly 6-fold improvement in sensitivity to 320 ± 20 nA μM
    MeSH term(s) Amino Acid Oxidoreductases/chemistry ; Amino Acid Oxidoreductases/metabolism ; Electrochemical Techniques/methods ; Enzymes, Immobilized/chemistry ; Enzymes, Immobilized/metabolism ; Fluorocarbon Polymers/chemistry ; Glutamic Acid/analysis ; Glutamic Acid/metabolism ; Hydrogen Peroxide/chemistry ; Micro-Electrical-Mechanical Systems ; Microelectrodes ; Oxidation-Reduction ; Polymers/chemistry
    Chemical Substances Enzymes, Immobilized ; Fluorocarbon Polymers ; Polymers ; perfluorosulfonic acid (39464-59-0) ; Glutamic Acid (3KX376GY7L) ; Hydrogen Peroxide (BBX060AN9V) ; Amino Acid Oxidoreductases (EC 1.4.-) ; L-glutamate oxidase (EC 1.4.3.11)
    Language English
    Publishing date 2020-01-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/c9an01969c
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