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  1. Article ; Online: Cooperation within the therapeutic relationship improves metacognitive functioning: preliminary findings.

    Farina, Benedetto / Liotti, Marianna / Imperatori, Claudio / Tombolini, Lucia / Gasperini, Elena / Mallozzi, Paola / Russo, Marianna / Simoncini Malucelli, Giorgia / Monticelli, Fabio

    Research in psychotherapy (Milano)

    2023  Volume 26, Issue 3

    Abstract: Both clinical observations and empirical data suggest that metacognitive functioning is a factor strongly associated with a good psychotherapeutic outcome. It has been suggested that some interpersonal social motivations (i.e., attachment and cooperation) ...

    Abstract Both clinical observations and empirical data suggest that metacognitive functioning is a factor strongly associated with a good psychotherapeutic outcome. It has been suggested that some interpersonal social motivations (i.e., attachment and cooperation) may be associated with different levels of metacognitive functioning also within the therapeutic relationship. The aim of this study was to explore the relationship between cooperation and metacognitive monitoring within 58 psychotherapy sessions from seven different patients. All patients were initially assessed through a detailed psychiatric interview. Patients' adult attachment styles were evaluated using the Attachment Style Questionnaire. The association between the activation of patients' interpersonal social motivations (e.g., cooperation and attachment) and the modifications of metacognitive abilities during sessions was investigated using the Assessing Interpersonal Motivations in Transcripts method and the Metacognition Assessment Scale have been used. Our results showed that the activation of the patient's cooperative system is positively associated with an increase in metacognitive functioning, while the activation of attachment is not. The results of the present study have important implications for clinicians: they give empirical support for the role of cooperation in fostering metacognition within the therapeutic relationship.
    Language English
    Publishing date 2023-11-08
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 2654235-3
    ISSN 2239-8031 ; 2239-8031
    ISSN (online) 2239-8031
    ISSN 2239-8031
    DOI 10.4081/ripppo.2023.712
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Morphological changes and protein degradation during the decomposition process of pig cadavers placed outdoors or in tents-a pilot study.

    Geissenberger, J / Amendt, J / Klampfer, J / Thuemmel, L / Jakob, L / Monticelli, F C / Steinbacher, P / Pittner, S

    Forensic science, medicine, and pathology

    2023  

    Abstract: The delimitation of the postmortem interval (PMI) is of utmost importance in forensic science. It is especially difficult to determine the PMI in advanced decomposition stages and/or when dead bodies are found under uncommon circumstances, such as tents, ...

    Abstract The delimitation of the postmortem interval (PMI) is of utmost importance in forensic science. It is especially difficult to determine the PMI in advanced decomposition stages and/or when dead bodies are found under uncommon circumstances, such as tents, or other (semi-) enclosed environments. In such cases, especially when insect access is restricted, morphological assessment of body decomposition is one of the remaining approaches for delimitation of the PMI. However, as this method allows only vague statements/indications about the PMI, it is required to develop new and more reliable methods. One of the most important candidates is the biochemical analysis of protein degradation. In this regard, it has been demonstrated that specific skeletal muscle protein degradation patterns characterize certain time points postmortem and thus can be used as markers for PMI estimation. In order to test this method in different micro-environments, a pilot study using ten pig carcasses was conducted in summer in Northern Germany. The cadavers were openly placed outside (freely accessible for insects), as well as enclosed in tents nearby, and left to decompose to investigate decomposition processes over a time course of 10 days. Muscle samples of the M. biceps femoris were collected on a regular basis and processed via SDS-PAGE and degradation patterns of selected proteins identified by Western blotting. In addition, morphological changes of the cadavers during decomposition were assessed using the total body score (TBS). Results showed that postmortem protein degradation patterns are largely consistent between treatment groups (open field versus tents) despite major morphological differences in the decomposition rate. This field study provides evidence that muscle protein degradation is mostly unaffected by different levels of exposure, making it a sufficient candidate for PMI delimitation under various circumstances.
    Language English
    Publishing date 2023-05-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2195904-3
    ISSN 1556-2891 ; 1547-769X
    ISSN (online) 1556-2891
    ISSN 1547-769X
    DOI 10.1007/s12024-023-00632-3
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  3. Article ; Online: Effect of temporary freezing on postmortem protein degradation patterns.

    Geissenberger, Janine / Pittner, S / Ehrenfellner, B / Jakob, L / Stoiber, W / Monticelli, F C / Steinbacher, P

    International journal of legal medicine

    2023  Volume 137, Issue 6, Page(s) 1803–1814

    Abstract: Background: A precise determination of time since death plays a major role in forensic routine. Currently available techniques for estimating the postmortem interval (PMI) are restricted to specific time periods or cannot be applied for individual case- ... ...

    Abstract Background: A precise determination of time since death plays a major role in forensic routine. Currently available techniques for estimating the postmortem interval (PMI) are restricted to specific time periods or cannot be applied for individual case-specific reasons. During recent years, it has been repeatedly demonstrated that Western blot analysis of postmortem muscle protein degradation can substantially contribute to overcome these limitations in cases with different background. Enabling to delimit time points at which certain marker proteins undergo distinct degradation events, the method has become a reasonable new tool for PMI delimitation under various forensic scenarios. However, additional research is yet required to improve our understanding of protein decomposition and how it is affected by intrinsic and extrinsic factors. Since there are temperature limits for proteolysis, and investigators are confronted with frozen corpses, investigation of the effects of freezing and thawing on postmortem protein decomposition in the muscle tissue is an important objective to firmly establish the new method. It is also important because freezing is often the only practical means to intermittently preserve tissue samples from both true cases and animal model research.
    Methods: Sets of dismembered pig hind limbs, either freshly detached non-frozen, or thawed after 4 months of freeze-storage (n = 6 each), were left to decompose under controlled conditions at 30 °C for 7 days and 10 days, respectively. Samples of the M. biceps femoris were regularly collected at predefined time points. All samples were processed via SDS-PAGE and Western blotting to identify the degradation patterns of previously characterized muscle proteins.
    Results: Western blots show that the proteins degrade predictably over time in precise patterns that are largely unaffected by the freeze-and-thaw process. Investigated proteins showed complete degradation of the native protein band, partly giving rise to degradation products present in distinct time phases of the decomposition process.
    Conclusion: This study provides substantial new information from a porcine model to assess the degree of bias that freezing and thawing induces on postmortem degradation of skeletal muscle proteins. Results support that a freeze-thaw cycle with prolonged storage in frozen state has no significant impact on the decomposition behavior. This will help to equip the protein degradation-based method for PMI determination with a robust applicability in the normal forensic setting.
    MeSH term(s) Animals ; Swine ; Proteolysis ; Freezing ; Postmortem Changes ; Autopsy ; Muscle Proteins/metabolism
    Chemical Substances Muscle Proteins
    Language English
    Publishing date 2023-06-03
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1055109-8
    ISSN 1437-1596 ; 0937-9827
    ISSN (online) 1437-1596
    ISSN 0937-9827
    DOI 10.1007/s00414-023-03024-y
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  4. Article ; Online: Ethyl glucuronide and ethanol concentrations in femoral blood, urine and vitreous humor from 117 autopsy cases.

    Neumann, Jasna / Keller, Thomas / Monticelli, Fabio / Beck, Olof / Böttcher, Michael

    Forensic science international

    2020  Volume 318, Page(s) 110567

    Abstract: Background: Vitreous humor (VH) is a specimen of great value in forensic investigations and is being used for evaluating possible post-mortem formation of ethanol. Ethyl glucuronide (EtG) is an ethanol metabolite that has found interest for the same ... ...

    Abstract Background: Vitreous humor (VH) is a specimen of great value in forensic investigations and is being used for evaluating possible post-mortem formation of ethanol. Ethyl glucuronide (EtG) is an ethanol metabolite that has found interest for the same purpose. Both compounds can be measured in VH and because of differences in rate of distribution and elimination they may offer complementary information.
    Methods: VH, femoral blood (FB) and urine were collected from 117 autopsy cases for forensic investigation. Ethanol was measured with headspace gas chromatography, while EtG was measured with liquid chromatography - tandem mass spectrometry.
    Results and conclusion: Ethanol was detected in all matrices in 39 cases, while EtG was present in 62 cases. The VH-FB and the VH-urine ethanol concentrations in the 39 cases were statistically correlated (p < 0.00001). In one case with an ethanol concentration of 0.11 g/L in FB, no ethanol was detected in VH and urine, and no EtG in any specimen, indicating a possible post-mortem formation. EtG was present in VH in more cases than in FB and urine. The correlation between the EtG concentrations in VH and FB was statistically significant (p < 0.0003) as was the case also for VH and urine (p < 0.001). The combined information on ethanol and EtG concentrations in the three matrices can be used to interpret alcohol drinking habit before death. This study confirms the value of using VH as a specimen in forensic investigations regarding recent exposure to ethanol. EtG can be used not only for investigating post-mortem ethanol formation but also for estimating recent alcohol drinking.
    MeSH term(s) Adult ; Aged ; Aged, 80 and over ; Alcohol Drinking ; Biomarkers/analysis ; Central Nervous System Depressants/analysis ; Chromatography, Liquid ; Ethanol/analysis ; Female ; Forensic Toxicology ; Glucuronates/analysis ; Humans ; Male ; Middle Aged ; Tandem Mass Spectrometry ; Vitreous Body/chemistry ; Young Adult
    Chemical Substances Biomarkers ; Central Nervous System Depressants ; Glucuronates ; ethyl glucuronide (17685-04-0) ; Ethanol (3K9958V90M)
    Language English
    Publishing date 2020-11-01
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 424042-x
    ISSN 1872-6283 ; 0379-0738
    ISSN (online) 1872-6283
    ISSN 0379-0738
    DOI 10.1016/j.forsciint.2020.110567
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  5. Article ; Online: Suitability of specific soft tissue swabs for the forensic identification of highly decomposed bodies.

    Helm, Katharina / Matzenauer, Christian / Neuhuber, Franz / Monticelli, Fabio / Meyer, Harald / Pittner, Stefan / Gotsmy, Walther

    International journal of legal medicine

    2021  Volume 135, Issue 4, Page(s) 1319–1327

    Abstract: When decomposition of a recovered body is fairly advanced, identification based on common morphologic features is often impossible. In these cases, short tandem repeat (STR) marker genotyping has established itself as a convenient and reliable ... ...

    Abstract When decomposition of a recovered body is fairly advanced, identification based on common morphologic features is often impossible. In these cases, short tandem repeat (STR) marker genotyping has established itself as a convenient and reliable alternative. However, at very progressed stages of decomposition, postmortem tissue putrefaction processes can decrease DNA yields considerably. Hence, not all types of tissue are equally suitable for successful STR marker-based postmortem identification. Bone or dental material is often analysed in corpses with advanced decompositional changes. However, processing of these materials is very elaborate and time and resource consuming. We have therefore focused on the suitableness of various types of soft tissue swabs, where DNA extraction is easier and faster. By sampling 28 bodies at various stages of decomposition, we evaluated the suitability of different tissues for genotyping at varying degrees of physical decay. This was achieved by a systematic classification of the sampled bodies by morphological scoring and subsequent analysis of multiple tissue swabs of the aortic wall, urinary bladder wall, brain, liver, oral mucosa and skeletal muscle. In summary, we found variable degrees of suitability of different types of soft tissue swabs for DNA-based identification. Swabs of the aortic wall, the urinary bladder wall and brain tissue yielded the best results - in descending order - even at advanced levels of decay.
    MeSH term(s) Aorta/chemistry ; Body Remains/chemistry ; Brain Chemistry ; DNA/isolation & purification ; DNA Degradation, Necrotic ; DNA Fingerprinting/methods ; Female ; Forensic Anthropology/methods ; Humans ; Liver/chemistry ; Male ; Microsatellite Repeats ; Mouth Mucosa/chemistry ; Muscle, Skeletal/chemistry ; Postmortem Changes ; Urinary Bladder/chemistry
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2021-04-20
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1055109-8
    ISSN 1437-1596 ; 0937-9827
    ISSN (online) 1437-1596
    ISSN 0937-9827
    DOI 10.1007/s00414-021-02601-3
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  6. Article ; Online: Dismembered porcine limbs as a proxy for postmortem muscle protein degradation.

    Geissenberger, J / Ehrenfellner, B / Monticelli, F C / Pittner, Stefan / Steinbacher, Peter

    International journal of legal medicine

    2021  Volume 135, Issue 4, Page(s) 1627–1636

    Abstract: The estimation of the postmortem interval (PMI) is of critical importance in forensic routine. The most frequently applied methods, however, are all restricted to specific time periods or must be excluded under certain circumstances. In the last years it ...

    Abstract The estimation of the postmortem interval (PMI) is of critical importance in forensic routine. The most frequently applied methods, however, are all restricted to specific time periods or must be excluded under certain circumstances. In the last years it has been shown that the analysis of muscle protein degradation has the potential to contribute to according delimitations in practice. In particular, upon biochemical analysis, the specific time points of degradation events provide reasonable markers for PMI delimitation. Nevertheless, considerable research is yet required to increase the understanding of protein decomposition and how it is affected by individual and environmental influencing factors. This is best investigated under standardized conditions, however, a considerate selection of proxies, regarding costs, effort, and expected outcome is required. Here, we use pigs to compare muscle protein decomposition in whole bodies and dismembered body parts (amputated hind limbs). Not only do experiments on body parts reduce the costs and allow easier handling in basic research, but also they aid to investigate the practical application of PMI estimation in dismembered body parts, or other extensive injuries, which are not unusual scenarios in crime investigation. Specifically, we investigated whether there are differences in the degradation rates of selected muscle proteins, sampled from dismembered legs and from hind limbs attached to whole pig bodies. Our results show distinct time-dependent degradation patterns of muscle proteins in a predictable manner regardless of sample origin. We are able to demonstrate that amputated hind limbs are suitable proxies for the analysis of muscle protein degradation, especially to investigate certain influencing factors and establish according standardized models.
    MeSH term(s) Animals ; Corpse Dismemberment ; Models, Animal ; Muscle Proteins/metabolism ; Muscle, Skeletal/metabolism ; Postmortem Changes ; Proteolysis ; Swine
    Chemical Substances Muscle Proteins
    Language English
    Publishing date 2021-05-06
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1055109-8
    ISSN 1437-1596 ; 0937-9827
    ISSN (online) 1437-1596
    ISSN 0937-9827
    DOI 10.1007/s00414-021-02571-6
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  7. Article ; Online: Cooperation in psychotherapy increases metacognitive abilities: a single-case study.

    Monticelli, Fabio / Imperatori, Claudio / Carcione, Antonino / Pedone, Roberto / Farina, Benedetto

    Rivista di psichiatria

    2019  Volume 53, Issue 6, Page(s) 336–340

    Abstract: Introduction: Both clinical observations and empirical data suggest that the ability to think about the mental states of themselves and others (i.e., metacognition) is a crucial factor strongly associated to the outcome of individual psychotherapies. ... ...

    Abstract Introduction: Both clinical observations and empirical data suggest that the ability to think about the mental states of themselves and others (i.e., metacognition) is a crucial factor strongly associated to the outcome of individual psychotherapies. Although it has been hypothesized that the activation of cooperation between patient and psychotherapist within psychotherapy sessions may increase metacognitive abilities, few data is still available to support this hypothesis.
    Methods: We explore the association between cooperation of patient and psychotherapist and the modifications of metacognition abilities along five sessions of a patient with a personality disorder using the Assessing Interpersonal Motivations in Transcripts method (AIMIT) and the Metacognition Assessment Scale (MAS).
    Results: Our data showed that the activation of cooperation was positively associated with both the MAS total scores and all MAS sub-scales.
    Discussion and conclusion: Our results demonstrate that the activation of the cooperation within therapeutic relationship can increase patient's metacognition and its subsystem (e.g. self-monitoring).
    MeSH term(s) Adult ; Dependent Personality Disorder/psychology ; Dependent Personality Disorder/therapy ; Female ; Humans ; Interpersonal Relations ; Metacognition ; Psychotherapy
    Language English
    Publishing date 2019-01-07
    Publishing country Italy
    Document type Case Reports ; Journal Article
    ZDB-ID 205570-3
    ISSN 2038-2502 ; 0035-6484
    ISSN (online) 2038-2502
    ISSN 0035-6484
    DOI 10.1708/3084.30768
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 1521: Show issues Location:
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  8. Article: Infrared pupillography in routine police traffic checks. Analysis of potential applications based on advanced statistical models. Infrarot-Pupillographie im Rahmen polizeilicher Routineverkehrskontrollen. Analyse der Anwendungsmöglichkeiten basierend auf modernen statistischen Modellen

    Hitzl, W. / Keller, T. / Monticelli, F.

    Rechtsmedizin

    2016  Volume 26, Issue 6, Page(s) 485

    Language German
    Document type Article
    ZDB-ID 1064911-6
    ISSN 0937-9819
    Database Current Contents Medicine

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  9. Article ; Online: Biomechanical assessment of various punching techniques.

    Adamec, Jiri / Hofer, Peter / Pittner, Stefan / Monticelli, Fabio / Graw, Matthias / Schöpfer, Jutta

    International journal of legal medicine

    2020  Volume 135, Issue 3, Page(s) 853–859

    Abstract: Punches without the use of instruments/objects are a common type of body violence and as such a frequent subject of medicolegal analyses. The assessment of the injuries occurred as well as of the potential of the assault to produce severe body harm is ... ...

    Abstract Punches without the use of instruments/objects are a common type of body violence and as such a frequent subject of medicolegal analyses. The assessment of the injuries occurred as well as of the potential of the assault to produce severe body harm is based on objective traces (especially the documented injuries of both parties involved) as well as the-often divergent-descriptions of the event. Quantitative data regarding the punching characteristics that could be used for the assessment are rare and originate mostly in sports science. The aim of this study was to provide physical data enabling/facilitating the assessment of various punching techniques. A total of 50 volunteers took part in our study (29 males and 21 females) and performed severe punches with the fist, with the small finger edge of the hand (karate chop), and with the open hand with both the dominant and the non-dominant hands in randomized order. The strikes were performed on a boxing pad attached to a KISTLER force plate (sampling frequency 10,000 Hz) mounted on a vertical wall. The punching velocity was defined as the hand velocity over the last 10 cm prior to the contact to the pad and ascertained by using a high-speed camera (2000 Hz). Apart from the strike velocity, the maximum force, the impulse (the integral of the force-time curve), the impact duration, and the effective mass of the punch (the ratio between the impulse and the strike velocity) were measured/calculated. The results show a various degree of dependence of the physical parameters of the strikes on the punching technique, gender, hand used, body weight, and other factors. On the other hand, a high degree of variability was observed that is likely attributable to individual punching capabilities. In a follow-up study, we plan to compare the "ordinary" persons with highly trained (boxers etc.) individuals. Even though the results must be interpreted with great caution and a direct transfer of the quantitative parameters to real-world situations is in general terms not possible, the study offers valuable insights and a solid basis for a qualified forensic medical/biomechanical assessment.
    MeSH term(s) Adult ; Biomechanical Phenomena ; Craniocerebral Trauma ; Female ; Forensic Sciences/methods ; Humans ; Kinetics ; Male ; Middle Aged ; Task Performance and Analysis ; Violence
    Language English
    Publishing date 2020-10-14
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1055109-8
    ISSN 1437-1596 ; 0937-9827
    ISSN (online) 1437-1596
    ISSN 0937-9827
    DOI 10.1007/s00414-020-02440-8
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  10. Article ; Online: Fatal course of undetected Ewing-like sarcoma in a 9-year-old boy with uncharacteristic clinical presentation.

    Gotsmy, Walther / Neumayer, Bettina / Kraus, Theo / Zellinger, Barbara / Neureiter, Daniel / Monticelli, Fabio / Meyer, Harald / Hofer, Peter

    Forensic science, medicine, and pathology

    2021  Volume 18, Issue 3, Page(s) 231–239

    Abstract: A 9-year-old boy collapsed shortly after complaining of shortness of breath. Despite immediate resuscitation measures, the boy died. A few weeks earlier, he had received antibiotic treatment for respiratory infection. However, the post-mortem examination ...

    Abstract A 9-year-old boy collapsed shortly after complaining of shortness of breath. Despite immediate resuscitation measures, the boy died. A few weeks earlier, he had received antibiotic treatment for respiratory infection. However, the post-mortem examination revealed an advanced tumor mass of the mediastinum with infiltration of vital structures, which was identified as a small blue round neoplasm with aspects of an extramedullary Ewing-like sarcoma by supplementary histological and immunohistochemical examinations.This dramatic clinical course of events shows that the possible presence of serious diseases should always be considered behind harmless symptoms, even in children.
    MeSH term(s) Child ; Male ; Humans ; Sarcoma, Ewing/diagnosis ; Sarcoma, Ewing/pathology ; Sarcoma ; Anti-Bacterial Agents
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2021-11-15
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 2195904-3
    ISSN 1556-2891 ; 1547-769X
    ISSN (online) 1556-2891
    ISSN 1547-769X
    DOI 10.1007/s12024-021-00437-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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