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  1. Article: Primary cilia in osteoblasts and osteocytes are required for skeletal development and mechanotransduction.

    Moraes de Lima Perini, Mariana / Pugh, Julie N / Scott, Elizabeth M / Bhula, Karan / Chirgwin, Austin / Reul, Olivia N / Berbari, Nicolas F / Li, Jiliang

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Primary cilia have been involved in the development and mechanosensation of various tissue types, including bone. In this study, we explored the mechanosensory role of primary cilia in bone growth and adaptation by examining two cilia specific genes, ... ...

    Abstract Primary cilia have been involved in the development and mechanosensation of various tissue types, including bone. In this study, we explored the mechanosensory role of primary cilia in bone growth and adaptation by examining two cilia specific genes, IFT88 and MKS5, required for proper cilia assembly and function. To analyze the role of primary cilia in osteoblasts, Osx1-GFP:Cre mice were bred with IFT88
    Language English
    Publishing date 2023-12-18
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.12.15.570609
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Anti-Siglec-15 Antibody Prevents Marked Bone Loss after Acute Spinal Cord Injury-Induced Immobilization in Rats.

    Peng, Yuanzhen / Langermann, Solomon / Kothari, Priyanka / Liu, Linda / Zhao, Wei / Hu, Yizhong / Chen, Zihao / Moraes de Lima Perini, Mariana / Li, Jiliang / Cao, Jay / Guo, X Edward / Chen, Lieping / Bauman, William A / Qin, Weiping

    JBMR plus

    2023  Volume 7, Issue 12, Page(s) e10825

    Abstract: Rapid and extensive sublesional bone loss after spinal cord injury (SCI) is a difficult medical problem that has been refractory to available interventions except the antiresorptive agent denosumab (DMAB). While DMAB has shown some efficacy in inhibiting ...

    Abstract Rapid and extensive sublesional bone loss after spinal cord injury (SCI) is a difficult medical problem that has been refractory to available interventions except the antiresorptive agent denosumab (DMAB). While DMAB has shown some efficacy in inhibiting bone loss, its concurrent inhibition of bone formation limits its use. Sialic acid-binding immunoglobulin-like lectin (Siglec)-15 is expressed on the cell surface of mature osteoclasts. Anti-Siglec-15 antibody (Ab) has been shown to inhibit osteoclast maturation and bone resorption while maintaining osteoblast activity, which is distinct from current antiresorptive agents that inhibit the activity of both osteoclasts and osteoblasts. The goal of the present study is to test a Siglec-15 Ab (NP159) as a new treatment option to prevent bone loss in an acute SCI model. To this end, 4-month-old male Wistar rats underwent complete spinal cord transection and were treated with either vehicle or NP159 at 20 mg/kg once every 2 weeks for 8 weeks. SCI results in significant decreases in bone mineral density (BMD, -18.7%), trabecular bone volume (-43.1%), trabecular connectivity (-59.7%), and bone stiffness (-76.3%) at the distal femur. Treatment with NP159 almost completely prevents the aforementioned deterioration of bone after SCI. Blood and histomorphometric analyses revealed that NP159 is able to greatly inhibit bone resorption while maintaining bone formation after acute SCI. In ex vivo cultures of bone marrow cells, NP159 reduces osteoclastogenesis while increasing osteoblastogenesis. In summary, treatment with NP159 almost fully prevents sublesional loss of BMD and metaphysis trabecular bone volume and preserves bone strength in a rat model of acute SCI. Because of its unique ability to reduce osteoclastogenesis and bone resorption while promoting osteoblastogenesis to maintain bone formation, Siglec-15 Ab may hold greater promise as a therapeutic agent, compared with the exclusively antiresorptive or anabolic agents that are currently used, in mitigating the striking bone loss that occurs after SCI or other conditions associated with severe immobilization. © 2023 The Authors.
    Language English
    Publishing date 2023-09-27
    Publishing country England
    Document type Journal Article
    ISSN 2473-4039
    ISSN (online) 2473-4039
    DOI 10.1002/jbm4.10825
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Injectable Acylhydrazone-Linked RAFT Polymer Hydrogels for Sustained Protein Release and Cell Encapsulation.

    Lin, Fang-Yi / Dimmitt, Nathan H / Moraes de Lima Perini, Mariana / Li, Jiliang / Lin, Chien-Chi

    Advanced healthcare materials

    2021  Volume 11, Issue 7, Page(s) e2101284

    Abstract: A new class of temperature responsive polymer, termed PADO, is synthesized by reversible addition-fragmentation chain-transfer polymerization. Synthesized from copolymerization of diacetone acrylamide (DAAM), di(ethylene glycol) ethyl ether acrylate, and ...

    Abstract A new class of temperature responsive polymer, termed PADO, is synthesized by reversible addition-fragmentation chain-transfer polymerization. Synthesized from copolymerization of diacetone acrylamide (DAAM), di(ethylene glycol) ethyl ether acrylate, and oligo(ethylene glycol) methyl ether acrylate, PADO polymer phase separates at temperature above its lower critical solution temperature (36-42 °C) due to enhanced hydrophobic interactions between the short ethylene glycol side chains. Solution of PADO polymers exhibit injectable shear-thinning properties and reach sol-gel transition rapidly (<5 min) at 37 °C. When the ketone moieties on DAAM are linked by adipic acid dihydrazdie, PADO polymers form crosslinked and injectable acylhydrazone hydrogels, which are hydrolytically degradable at a mild acidic environment owing to the pH sensitive acylhydrazone bonds. The pH-responsive degradation kinetics can be controlled by tuning polymer contents and ketone/hydrazide ratio. Importantly, the injectable PADO hydrogels are highly cytocompatible and can be easily formulated for pH-responsive sustained protein delivery.
    MeSH term(s) Cell Encapsulation ; Ethylene Glycols ; Hydrogels/chemistry ; Ketones ; Polymers ; Proteins ; Temperature
    Chemical Substances Ethylene Glycols ; Hydrogels ; Ketones ; Polymers ; Proteins
    Language English
    Publishing date 2021-10-13
    Publishing country Germany
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2649576-4
    ISSN 2192-2659 ; 2192-2640
    ISSN (online) 2192-2659
    ISSN 2192-2640
    DOI 10.1002/adhm.202101284
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Characterization and assessment of lung and bone marrow derived endothelial cells and their bone regenerative potential.

    Moraes de Lima Perini, Mariana / Valuch, Conner R / Dadwal, Ushashi C / Awosanya, Olatundun D / Mostardo, Sarah L / Blosser, Rachel J / Knox, Adam M / McGuire, Anthony C / Battina, Hanisha L / Nazzal, Murad / Kacena, Melissa A / Li, Jiliang

    Frontiers in endocrinology

    2022  Volume 13, Page(s) 935391

    Abstract: Angiogenesis is important for successful fracture repair. Aging negatively affects the number and activity of endothelial cells (ECs) and subsequently leads to impaired bone healing. We previously showed that implantation of lung-derived endothelial ... ...

    Abstract Angiogenesis is important for successful fracture repair. Aging negatively affects the number and activity of endothelial cells (ECs) and subsequently leads to impaired bone healing. We previously showed that implantation of lung-derived endothelial cells (LECs) improved fracture healing in rats. In this study, we characterized and compared neonatal lung and bone marrow-derived endothelial cells (neonatal LECs and neonatal BMECs) and further asses3sed if implantation of neonatal BMECs could enhance bone healing in both young and aged mice. We assessed neonatal EC tube formation, proliferation, and wound migration ability
    MeSH term(s) Animals ; Bone Marrow ; Bone Regeneration ; Collagen ; Disease Models, Animal ; Endothelial Cells ; Fractures, Bone ; Lung ; Mice ; Rats
    Chemical Substances Collagen (9007-34-5)
    Language English
    Publishing date 2022-08-31
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2022.935391
    Database MEDical Literature Analysis and Retrieval System OnLINE

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