LIVIVO - Search results -

Search results

Result 1 - 10 of total 44

Search options

Article ; Online: Protocol for the isolation and culture of microglia, astrocytes, and neurons from the same mouse brain.

Leites, Elvira P / Morais, Vanessa A

STAR protocols

2024 Volume 5, Issue 1, Page(s) 102804

Abstract: Studying the intrinsic properties of microglia, astrocytes, and neurons is essential to our understanding of brain function. Here, we present a protocol to isolate and culture these neural cells from the same mouse brain. Using immunocapture magnetic ... ...

Abstract	Studying the intrinsic properties of microglia, astrocytes, and neurons is essential to our understanding of brain function. Here, we present a protocol to isolate and culture these neural cells from the same mouse brain. Using immunocapture magnetic beads, we describe steps for dissociating, cleaning, and sequentially separating brains from 9-day-old mice into microglia, astrocytes, and neurons. Following these detailed procedures for seeding and culturing of isolated cells, we can address critical questions related to brain function.
MeSH term(s)	Mice ; Animals ; Astrocytes ; Microglia ; Neurons/physiology ; Brain ; Immunomagnetic Separation
Language	English
Publishing date	2024-01-11
Publishing country	United States
Document type	Journal Article
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2023.102804
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Protocol for the isolation and culture of microglia, astrocytes, and neurons from the same mouse brain.

Leites, Elvira P / Morais, Vanessa A

STAR protocols

2024 Volume 5, Issue 1, Page(s) 102869

Language	English
Publishing date	2024-02-01
Publishing country	United States
Document type	Published Erratum
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2024.102869
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Lineage-specific changes in mitochondrial properties during neural stem cell differentiation.

Soares, Rita / Lourenço, Diogo M / Mota, Isa F / Sebastião, Ana M / Xapelli, Sara / Morais, Vanessa A

Life science alliance

2024 Volume 7, Issue 7

Abstract: Neural stem cells (NSCs) reside in discrete regions of the adult mammalian brain where they can differentiate into neurons, astrocytes, and oligodendrocytes. Several studies suggest that mitochondria have a major role in regulating NSC fate. Here, we ... ...

Abstract	Neural stem cells (NSCs) reside in discrete regions of the adult mammalian brain where they can differentiate into neurons, astrocytes, and oligodendrocytes. Several studies suggest that mitochondria have a major role in regulating NSC fate. Here, we evaluated mitochondrial properties throughout NSC differentiation and in lineage-specific cells. For this, we used the neurosphere assay model to isolate, expand, and differentiate mouse subventricular zone postnatal NSCs. We found that the levels of proteins involved in mitochondrial fusion (Mitofusin [Mfn] 1 and Mfn 2) increased, whereas proteins involved in fission (dynamin-related protein 1 [DRP1]) decreased along differentiation. Importantly, changes in mitochondrial dynamics correlated with distinct patterns of mitochondrial morphology in each lineage. Particularly, we found that the number of branched and unbranched mitochondria increased during astroglial and neuronal differentiation, whereas the area occupied by mitochondrial structures significantly reduced with oligodendrocyte maturation. In addition, comparing the three lineages, neurons revealed to be the most energetically flexible, whereas astrocytes presented the highest ATP content. Our work identified putative mitochondrial targets to enhance lineage-directed differentiation of mouse subventricular zone-derived NSCs.
MeSH term(s)	Animals ; Neural Stem Cells/metabolism ; Neural Stem Cells/cytology ; Mitochondria/metabolism ; Mice ; Cell Differentiation/genetics ; Cell Lineage/genetics ; Astrocytes/metabolism ; Astrocytes/cytology ; Mitochondrial Dynamics ; Oligodendroglia/metabolism ; Oligodendroglia/cytology ; Neurons/metabolism ; Neurons/cytology ; Cells, Cultured ; Mitochondrial Proteins/metabolism ; Mitochondrial Proteins/genetics ; GTP Phosphohydrolases/metabolism ; GTP Phosphohydrolases/genetics ; Neurogenesis ; Lateral Ventricles/cytology ; Lateral Ventricles/metabolism ; Dynamins
Chemical Substances	Mfn1 protein, mouse (EC 3.6.1.-) ; Mfn2 protein, mouse (EC 3.6.1.-) ; Dnm1l protein, mouse (EC 3.6.5.5) ; Mitochondrial Proteins ; GTP Phosphohydrolases (EC 3.6.1.-) ; Dynamins (EC 3.6.5.5)
Language	English
Publishing date	2024-04-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2575-1077
ISSN (online)	2575-1077
DOI	10.26508/lsa.202302473
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Reduced penetrance of Parkinson's disease models

Morais, Vanessa A. / Vos, Melissa

Medizinische Genetik

2022 Volume 34, Issue 2, Page(s) 117

Language	German
Document type	Article
ZDB-ID	1083376-6
ISSN	0936-5931
Database	Current Contents Medicine

In stock of ZB MED Cologne/Königswinter

Zs.A 3294: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Synapses: The Brain's Energy-Demanding Sites.

Faria-Pereira, Andreia / Morais, Vanessa A

International journal of molecular sciences

2022 Volume 23, Issue 7

Abstract: The brain is one of the most energy-consuming organs in the mammalian body, and synaptic transmission is one of the major contributors. To meet these energetic requirements, the brain primarily uses glucose, which can be metabolized through glycolysis ... ...

Abstract	The brain is one of the most energy-consuming organs in the mammalian body, and synaptic transmission is one of the major contributors. To meet these energetic requirements, the brain primarily uses glucose, which can be metabolized through glycolysis and/or mitochondrial oxidative phosphorylation. The relevance of these two energy production pathways in fulfilling energy at presynaptic terminals has been the subject of recent studies. In this review, we dissect the balance of glycolysis and oxidative phosphorylation to meet synaptic energy demands in both resting and stimulation conditions. Besides ATP output needs, mitochondria at synapse are also important for calcium buffering and regulation of reactive oxygen species. These two mitochondrial-associated pathways, once hampered, impact negatively on neuronal homeostasis and synaptic activity. Therefore, as mitochondria assume a critical role in synaptic homeostasis, it is becoming evident that the synaptic mitochondria population possesses a distinct functional fingerprint compared to other brain mitochondria. Ultimately, dysregulation of synaptic bioenergetics through glycolytic and mitochondrial dysfunctions is increasingly implicated in neurodegenerative disorders, as one of the first hallmarks in several of these diseases are synaptic energy deficits, followed by synapse degeneration.
MeSH term(s)	Animals ; Brain/metabolism ; Energy Metabolism/physiology ; Mammals ; Presynaptic Terminals/metabolism ; Synapses/metabolism ; Synaptic Transmission/physiology
Language	English
Publishing date	2022-03-26
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23073627
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: BrainPhys Neuronal Media Support Physiological Function of Mitochondria in Mouse Primary Neuronal Cultures.

Faria-Pereira, Andreia / Temido-Ferreira, Mariana / Morais, Vanessa A

Frontiers in molecular neuroscience

2022 Volume 15, Page(s) 837448

Abstract: ... In ... ...

Abstract	In vitro
Language	English
Publishing date	2022-06-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2452967-9
ISSN	1662-5099
ISSN	1662-5099
DOI	10.3389/fnmol.2022.837448
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Mitochondrial quality control pathways: PINK1 acts as a gatekeeper.

Leites, Elvira P / Morais, Vanessa A

Biochemical and biophysical research communications

2017 Volume 500, Issue 1, Page(s) 45–50

Abstract: Mitochondria have a pivotal role in the maintenance of cell homeostasis and survival. Mitochondria are involved in processes such as ATP production, reactive oxygen species production, apoptosis induction, calcium homeostasis and protein degradation. ... ...

Abstract	Mitochondria have a pivotal role in the maintenance of cell homeostasis and survival. Mitochondria are involved in processes such as ATP production, reactive oxygen species production, apoptosis induction, calcium homeostasis and protein degradation. Thus, mechanisms that regulate the intrinsic quality of mitochondria have a crucial role in dictating overall cell fate. The importance of these well-regulated mechanisms is highlighted in disease scenarios such as neurodegeneration, cancer and neuromuscular atrophy. How mitochondria senses and regulates their intrinsic quality control, and consequently cell survival, is still not fully understood. In this review, we discuss the pathways that are at present considered as state-of-the-art for mitochondria quality control regulation, and highlight a mitochondrial protein-PINK1-that has revealed to act as a mitochondrial gatekeeper able to sense the presence of healthy or damaged mitochondria.
MeSH term(s)	Apoptosis/genetics ; GTP Phosphohydrolases/genetics ; GTP Phosphohydrolases/metabolism ; Gene Expression Regulation ; Homeostasis/genetics ; Humans ; Microtubule-Associated Proteins/genetics ; Microtubule-Associated Proteins/metabolism ; Mitochondria/genetics ; Mitochondria/metabolism ; Mitochondria/pathology ; Mitochondrial Degradation/genetics ; Mitochondrial Proteins/genetics ; Mitochondrial Proteins/metabolism ; Muscular Atrophy/genetics ; Muscular Atrophy/metabolism ; Muscular Atrophy/pathology ; Neoplasms/genetics ; Neoplasms/metabolism ; Neoplasms/pathology ; Neurodegenerative Diseases/genetics ; Neurodegenerative Diseases/metabolism ; Neurodegenerative Diseases/pathology ; Protein Kinases/genetics ; Protein Kinases/metabolism ; Reactive Oxygen Species/metabolism ; Signal Transduction ; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/metabolism ; rho GTP-Binding Proteins/genetics ; rho GTP-Binding Proteins/metabolism
Chemical Substances	Microtubule-Associated Proteins ; Mitochondrial Proteins ; Reactive Oxygen Species ; Ubiquitin-Protein Ligases (EC 2.3.2.27) ; parkin protein (EC 2.3.2.27) ; Protein Kinases (EC 2.7.-) ; PTEN-induced putative kinase (EC 2.7.11.1) ; GTP Phosphohydrolases (EC 3.6.1.-) ; MFN2 protein, human (EC 3.6.1.-) ; RHOT1 protein, human (EC 3.6.1.-) ; rho GTP-Binding Proteins (EC 3.6.5.2) ; DNM1L protein, human (EC 3.6.5.5)
Language	English
Publishing date	2017-06-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2017.06.096
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 116: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Local mitochondrial replication in the periphery of neurons requires the eEF1A1 protein and thetranslation of nuclear-encoded proteins.

Cardanho-Ramos, Carlos / Simões, Rúben Alves / Wang, Yi-Zhi / Faria-Pereira, Andreia / Bomba-Warczak, Ewa / Craessaerts, Katleen / Spinazzi, Marco / Savas, Jeffrey N / Morais, Vanessa A

iScience

2024 Volume 27, Issue 4, Page(s) 109136

Abstract: In neurons, it is commonly assumed that mitochondrial replication only occurs in the cell body, after which the mitochondria must travel to the neuron's periphery. However, while mitochondrial DNA replication has been observed to occur away from the cell ...

Abstract	In neurons, it is commonly assumed that mitochondrial replication only occurs in the cell body, after which the mitochondria must travel to the neuron's periphery. However, while mitochondrial DNA replication has been observed to occur away from the cell body, the specific mechanisms involved remain elusive. Using EdU-labelling in mouse primary neurons, we developed a tool to determine the mitochondrial replication rate. Taking of advantage of microfluidic devices, we confirmed that mitochondrial replication also occurs locally in the periphery of neurons. To achieve this, mitochondria require
Language	English
Publishing date	2024-02-05
Publishing country	United States
Document type	Journal Article
ISSN	2589-0042
ISSN (online)	2589-0042
DOI	10.1016/j.isci.2024.109136
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: CD4

Cortes-Figueiredo, Filipe / Asseyer, Susanna / Chien, Claudia / Zimmermann, Hanna G / Ruprecht, Klemens / Schmitz-Hübsch, Tanja / Bellmann-Strobl, Judith / Paul, Friedemann / Morais, Vanessa A

Scientific reports

2024 Volume 14, Issue 1, Page(s) 7507

Abstract: Multiple Sclerosis (MS) is a chronic autoimmune demyelinating disease of the central nervous system (CNS), with a largely unknown etiology, where mitochondrial dysfunction likely contributes to neuroaxonal loss and brain atrophy. Mirroring the CNS, ... ...

Abstract	Multiple Sclerosis (MS) is a chronic autoimmune demyelinating disease of the central nervous system (CNS), with a largely unknown etiology, where mitochondrial dysfunction likely contributes to neuroaxonal loss and brain atrophy. Mirroring the CNS, peripheral immune cells from patients with MS, particularly CD4
MeSH term(s)	Humans ; Multiple Sclerosis/genetics ; T-Lymphocytes ; Cross-Sectional Studies ; Longitudinal Studies ; Retrospective Studies ; Multiple Sclerosis, Relapsing-Remitting/genetics ; Demyelinating Diseases ; DNA, Mitochondrial/genetics ; CD4-Positive T-Lymphocytes ; Genotype
Chemical Substances	DNA, Mitochondrial
Language	English
Publishing date	2024-03-29
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-57592-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Mitochondrial Metabolism Drives Low-density Lipoprotein-induced Breast Cancer Cell Migration.

Nóbrega-Pereira, Sandrina / Santos, Francisco / Oliveira Santos, Miguel / Serafim, Teresa L / Lopes, Ana Patrícia / Coutinho, Diogo / Carvalho, Filipa S / Domingues, Rosário M / Domingues, Pedro / Bernardes de Jesus, Bruno / Morais, Vanessa A / Dias, Sérgio

Cancer research communications

2023 Volume 3, Issue 4, Page(s) 709–724

Abstract: Most cancer-related deaths are due to metastases. Systemic factors, such as lipid-enriched environments [as low-density lipoprotein (LDL)-cholesterol], favor breast cancer, including triple-negative breast cancer (TNBC) metastasis formation. Mitochondria ...

Abstract	Most cancer-related deaths are due to metastases. Systemic factors, such as lipid-enriched environments [as low-density lipoprotein (LDL)-cholesterol], favor breast cancer, including triple-negative breast cancer (TNBC) metastasis formation. Mitochondria metabolism impacts TNBC invasive behavior but its involvement in a lipid-enriched setting is undisclosed. Here we show that LDL increases lipid droplets, induces CD36 and augments TNBC cells migration and invasion Significance: LDL induces breast cancer cell migration that relies on CD36 for mitochondrial metabolism and network remodeling, providing an antimetastatic metabolic strategy.
MeSH term(s)	Humans ; Lipoproteins, LDL/pharmacology ; Triple Negative Breast Neoplasms/metabolism ; Reactive Oxygen Species ; Fatty Acids/pharmacology ; Cell Movement
Chemical Substances	Lipoproteins, LDL ; Reactive Oxygen Species ; Fatty Acids
Language	English
Publishing date	2023-04-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2767-9764
ISSN (online)	2767-9764
DOI	10.1158/2767-9764.CRC-22-0394
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED