LIVIVO - Search results -

Search results

Result 1 - 10 of total 19

Search options

Article ; Online: Uncovering Surface Penetration by Enterococci From Urinary Tract Infection Patients.

Sansone, Stephanie / Ramos, Yusibeska / Segal, Saya / Asfaw, Tirsit S / Morales, Diana K

Urogynecology (Philadelphia, Pa.)

2024 Volume 30, Issue 3, Page(s) 320–329

Abstract: Importance: The relationship between Enterococcus faecalis vaginal colonization and urinary tract infections (UTIs) remains uncertain.: Objective: We aimed to evaluate the surface invasion capability of E faecalis isolates from patients with and ... ...

Abstract	Importance: The relationship between Enterococcus faecalis vaginal colonization and urinary tract infections (UTIs) remains uncertain. Objective: We aimed to evaluate the surface invasion capability of E faecalis isolates from patients with and without UTIs as a potential readout of pathogenicity. Study design: Participants were females from urogynecology clinics, comprising symptomatic UTI and asymptomatic non-UTI patients, categorized by the presence or absence of E faecalis-positive cultures identified via standard urine culture techniques. Vaginal and urine samples from patients were plated on enterococci selective medium, and E faecalis isolates detected in both cohorts were species specific identified using 16S rRNA sequencing. Clinical isolates were inoculated on semisolid media, and both external colonies and underneath colony prints formed by agar-penetrating enterococci were imaged. External growth and invasiveness were quantified by determining colony-forming units of the noninvading and agar-penetrating cells and compared with the E faecalis OG1RF. Results: We selected E faecalis isolates from urine and vaginal samples of 4 patients with and 4 patients without UTIs. Assays demonstrated that most isolates formed similarly sized external colonies with comparable colony-forming unit. Surface invasion differed across patients and isolation sites compared with OG1RF. The vaginal isolate from UTI patient 1, who had the most recurrences, exhibited significantly greater agar-invading capacity compared with OG1RF. Conclusions: Our pilot study indicates that ex vivo invasion assays may unveil virulence traits in E faecalis from UTI patients. Enhanced enterococcal surface penetration could increase urogenital invasion risk. Further research is needed to correlate penetration with disease severity in a larger patient group.
MeSH term(s)	Female ; Humans ; Male ; Enterococcus/genetics ; Agar ; Pilot Projects ; RNA, Ribosomal, 16S ; Urinary Tract Infections
Chemical Substances	Agar (9002-18-0) ; RNA, Ribosomal, 16S
Language	English
Publishing date	2024-03-06
Publishing country	United States
Document type	Journal Article
ISSN	2771-1897
ISSN (online)	2771-1897
DOI	10.1097/SPV.0000000000001476
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Sugarcoating it: Enterococcal polysaccharides as key modulators of host-pathogen interactions.

Ramos, Yusibeska / Sansone, Stephanie / Morales, Diana K

PLoS pathogens

2021 Volume 17, Issue 9, Page(s) e1009822

MeSH term(s)	Enterococcus faecalis/metabolism ; Enterococcus faecium/metabolism ; Gastrointestinal Microbiome ; Gram-Positive Bacterial Infections/metabolism ; Host-Pathogen Interactions/physiology ; Humans ; Polysaccharides/metabolism
Chemical Substances	Polysaccharides
Language	English
Publishing date	2021-09-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1009822
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Exopolysaccharide-mediated surface penetration as new virulence trait in

Ramos, Yusibeska / Morales, Diana K

Communicative & integrative biology

2019 Volume 12, Issue 1, Page(s) 144–147

Abstract: Enterococcus ... ...

Abstract	Enterococcus faecalis
Language	English
Publishing date	2019-08-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2451097-X
ISSN	1942-0889
ISSN	1942-0889
DOI	10.1080/19420889.2019.1657373
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Light/Dark and Temperature Cycling Modulate Metabolic Electron Flow in Pseudomonas aeruginosa Biofilms.

Kahl, Lisa Juliane / Eckartt, Kelly N / Morales, Diana K / Price-Whelan, Alexa / Dietrich, Lars E P

mBio

2022 Volume 13, Issue 4, Page(s) e0140722

Abstract: Sunlight drives phototrophic metabolism, which affects redox conditions and produces substrates for nonphototrophs. These environmental parameters fluctuate daily due to Earth's rotation, and nonphototrophic organisms can therefore benefit from the ... ...

Abstract	Sunlight drives phototrophic metabolism, which affects redox conditions and produces substrates for nonphototrophs. These environmental parameters fluctuate daily due to Earth's rotation, and nonphototrophic organisms can therefore benefit from the ability to respond to, or even anticipate, such changes. Circadian rhythms, such as daily changes in body temperature, in host organisms can also affect local conditions for colonizing bacteria. Here, we investigated the effects of light/dark and temperature cycling on biofilms of the opportunistic pathogen Pseudomonas aeruginosa PA14. We grew biofilms in the presence of a respiratory indicator dye and found that enhanced dye reduction occurred in biofilm zones that formed during dark intervals and at lower temperatures. This pattern formation occurred with cycling of blue, red, or far-red light, and a screen of mutants representing potential sensory proteins identified two with defects in pattern formation, specifically under red light cycling. We also found that the physiological states of biofilm subzones formed under specific light and temperature conditions were retained during subsequent condition cycling. Light/dark and temperature cycling affected expression of genes involved in primary metabolic pathways and redox homeostasis, including those encoding electron transport chain components. Consistent with this, we found that
MeSH term(s)	Animals ; Biofilms ; Electrons ; Oxidation-Reduction ; Pseudomonas aeruginosa/metabolism ; Temperature
Language	English
Publishing date	2022-08-08
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mbio.01407-22
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Remodeling of the Enterococcal Cell Envelope during Surface Penetration Promotes Intrinsic Resistance to Stress.

Ramos, Yusibeska / Sansone, Stephanie / Hwang, Sung-Min / Sandoval, Tito A / Zhu, Mengmeng / Zhang, Guoan / Cubillos-Ruiz, Juan R / Morales, Diana K

mBio

2022 Volume 13, Issue 6, Page(s) e0229422

Abstract: Enterococcus faecalis is a normal commensal of the human gastrointestinal tract (GIT). However, upon disruption of gut homeostasis, this nonmotile bacterium can egress from its natural niche and spread to distal organs. While this translocation process ... ...

Abstract	Enterococcus faecalis is a normal commensal of the human gastrointestinal tract (GIT). However, upon disruption of gut homeostasis, this nonmotile bacterium can egress from its natural niche and spread to distal organs. While this translocation process can lead to life-threatening systemic infections, the underlying mechanisms remain largely unexplored. Our prior work showed that E. faecalis migration across diverse surfaces requires the formation of matrix-covered multicellular aggregates and the synthesis of exopolysaccharides, but how enterococcal cells are reprogrammed during this process is unknown. Whether surface penetration endows E. faecalis with adaptive advantages is also uncertain. Here, we report that surface penetration promotes the generation of a metabolically and phenotypically distinct E. faecalis population with an enhanced capacity to endure various forms of extracellular stress. Surface-invading enterococci demonstrated major ultrastructural alterations in their cell envelope characterized by increased membrane glycolipid content. These changes were accompanied by marked induction of specific transcriptional programs enhancing cell envelope biogenesis and glycolipid metabolism. Notably, the surface-invading population demonstrated superior tolerance to membrane-damaging antimicrobials, including daptomycin and β-defensins produced by epithelial cells. Genetic mutations impairing glycolipid biosynthesis sensitized E. faecalis to envelope stressors and reduced the ability of this bacterium to penetrate semisolid surfaces and translocate through human intestinal epithelial cell monolayers. Our study reveals that surface penetration induces distinct transcriptional, metabolic, and ultrastructural changes that equip E. faecalis with enhanced capacity to resist external stressors and thrive in its surrounding environment.
MeSH term(s)	Humans ; Cell Membrane/metabolism ; Daptomycin/pharmacology ; Cell Wall/metabolism ; Enterococcus faecalis/genetics ; Biofilms ; Anti-Bacterial Agents/pharmacology
Chemical Substances	Daptomycin (NWQ5N31VKK) ; Anti-Bacterial Agents
Language	English
Publishing date	2022-11-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mbio.02294-22
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Candida albicans interactions with bacteria in the context of human health and disease.

Morales, Diana K / Hogan, Deborah A

PLoS pathogens

2010 Volume 6, Issue 4, Page(s) e1000886

MeSH term(s)	Bacterial Physiological Phenomena ; Biofilms ; Candida albicans/physiology ; Humans
Language	English
Publishing date	2010-04-29
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1000886
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Transgelin 2 guards T cell lipid metabolic programming and anti-tumor function.

Research square

2023

Abstract: Mounting effective immunity against pathogens and tumors relies on the successful metabolic programming of T cells by extracellular fatty ... ...

Abstract	Mounting effective immunity against pathogens and tumors relies on the successful metabolic programming of T cells by extracellular fatty acids
Language	English
Publishing date	2023-12-14
Publishing country	United States
Document type	Preprint
DOI	10.21203/rs.3.rs-3683989/v1
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Inflammatory ER stress responses dictate the immunopathogenic progression of systemic candidiasis.

Awasthi, Deepika / Chopra, Sahil / Cho, Byuri A / Emmanuelli, Alexander / Sandoval, Tito A / Hwang, Sung-Min / Chae, Chang-Suk / Salvagno, Camilla / Tan, Chen / Vasquez-Urbina, Liliana / Fernandez Rodriguez, Jose J / Santagostino, Sara F / Iwawaki, Takao / Romero-Sandoval, E Alfonso / Crespo, Mariano Sanchez / Morales, Diana K / Iliev, Iliyan D / Hohl, Tobias M / Cubillos-Ruiz, Juan R

The Journal of clinical investigation

2023 Volume 133, Issue 17

Abstract: Recognition of pathogen-associated molecular patterns can trigger the inositol-requiring enzyme 1 α (IRE1α) arm of the endoplasmic reticulum (ER) stress response in innate immune cells. This process maintains ER homeostasis and also coordinates diverse ... ...

Abstract	Recognition of pathogen-associated molecular patterns can trigger the inositol-requiring enzyme 1 α (IRE1α) arm of the endoplasmic reticulum (ER) stress response in innate immune cells. This process maintains ER homeostasis and also coordinates diverse immunomodulatory programs during bacterial and viral infections. However, the role of innate IRE1α signaling in response to fungal pathogens remains elusive. Here, we report that systemic infection with the human opportunistic fungal pathogen Candida albicans induced proinflammatory IRE1α hyperactivation in myeloid cells that led to fatal kidney immunopathology. Mechanistically, simultaneous activation of the TLR/IL-1R adaptor protein MyD88 and the C-type lectin receptor dectin-1 by C. albicans induced NADPH oxidase-driven generation of ROS, which caused ER stress and IRE1α-dependent overexpression of key inflammatory mediators such as IL-1β, IL-6, chemokine (C-C motif) ligand 5 (CCL5), prostaglandin E2 (PGE2), and TNF-α. Selective ablation of IRE1α in leukocytes, or treatment with an IRE1α pharmacological inhibitor, mitigated kidney inflammation and prolonged the survival of mice with systemic C. albicans infection. Therefore, controlling IRE1α hyperactivation may be useful for impeding the immunopathogenic progression of disseminated candidiasis.
MeSH term(s)	Humans ; Animals ; Mice ; Protein Serine-Threonine Kinases/metabolism ; Endoribonucleases/metabolism ; Candidiasis ; Endoplasmic Reticulum Stress ; Candida albicans ; Toll-Like Receptors/metabolism ; Adaptor Proteins, Signal Transducing/metabolism
Chemical Substances	Protein Serine-Threonine Kinases (EC 2.7.11.1) ; Endoribonucleases (EC 3.1.-) ; Toll-Like Receptors ; Adaptor Proteins, Signal Transducing
Language	English
Publishing date	2023-09-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	3067-3
ISSN	1558-8238 ; 0021-9738
ISSN (online)	1558-8238
ISSN	0021-9738
DOI	10.1172/JCI167359
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ua VI Zs.184: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Candida albicans-produced farnesol stimulates Pseudomonas quinolone signal production in LasR-defective Pseudomonas aeruginosa strains.

Cugini, Carla / Morales, Diana K / Hogan, Deborah A

Microbiology (Reading, England)

2010 Volume 156, Issue Pt 10, Page(s) 3096–3107

Abstract: Candida albicans has been previously shown to stimulate the production of Pseudomonas aeruginosa phenazine toxins in dual-species colony biofilms. Here, we report that P. aeruginosa lasR mutants, which lack the master quorum sensing system regulator, ... ...

Abstract	Candida albicans has been previously shown to stimulate the production of Pseudomonas aeruginosa phenazine toxins in dual-species colony biofilms. Here, we report that P. aeruginosa lasR mutants, which lack the master quorum sensing system regulator, regain the ability to produce quorum-sensing-regulated phenazines when cultured with C. albicans. Farnesol, a signalling molecule produced by C. albicans, was sufficient to stimulate phenazine production in LasR(-) laboratory strains and clinical isolates. P. aeruginosa ΔlasR mutants are defective in production of the Pseudomonas quinolone signal (PQS) due to their inability to properly induce pqsH, which encodes the enzyme necessary for the last step in PQS biosynthesis. We show that expression of pqsH in a ΔlasR strain was sufficient to restore PQS production, and that farnesol restored pqsH expression in ΔlasR mutants. The farnesol-mediated increase in pqsH required RhlR, a transcriptional regulator downstream of LasR, and farnesol led to higher levels of N-butyryl-homoserine lactone, the small molecule activator of RhlR. Farnesol promotes the production of reactive oxygen species (ROS) in a variety of species. Because the antioxidant N-acetylcysteine suppressed farnesol-induced RhlR activity in LasR(-) strains, and hydrogen peroxide was sufficient to restore PQS production in las mutants, we propose that ROS are responsible for the activation of downstream portions of this quorum sensing pathway. LasR mutants frequently arise in the lungs of patients chronically infected with P. aeruginosa. The finding that C. albicans, farnesol or ROS stimulate virulence factor production in lasR strains provides new insight into the virulence potential of these strains.
MeSH term(s)	4-Butyrolactone/analogs & derivatives ; 4-Butyrolactone/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Candida albicans/chemistry ; Coculture Techniques ; Farnesol/metabolism ; Gene Deletion ; Gene Expression Regulation, Bacterial ; Mutation ; Oxidative Stress ; Phenazines/metabolism ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/metabolism ; Pyocyanine/analysis ; Quinolones/metabolism ; Quorum Sensing ; Reactive Oxygen Species/metabolism ; Signal Transduction ; Trans-Activators/genetics ; Trans-Activators/metabolism ; Virulence Factors/biosynthesis
Chemical Substances	Bacterial Proteins ; LasR protein, Pseudomonas aeruginosa ; Phenazines ; Quinolones ; Reactive Oxygen Species ; Trans-Activators ; Virulence Factors ; homoserine lactone (1192-20-7) ; Farnesol (4602-84-0) ; Pyocyanine (9OQM399341) ; 4-Butyrolactone (OL659KIY4X)
Language	English
Publishing date	2010-07-23
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1180712-x
ISSN	1465-2080 ; 1350-0872
ISSN (online)	1465-2080
ISSN	1350-0872
DOI	10.1099/mic.0.037911-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.140: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: PolyGlcNAc-containing exopolymers enable surface penetration by non-motile Enterococcus faecalis.

Ramos, Yusibeska / Rocha, Jorge / Hael, Ana L / van Gestel, Jordi / Vlamakis, Hera / Cywes-Bentley, Colette / Cubillos-Ruiz, Juan R / Pier, Gerald B / Gilmore, Michael S / Kolter, Roberto / Morales, Diana K

PLoS pathogens

2019 Volume 15, Issue 2, Page(s) e1007571

Abstract: Bacterial pathogens have evolved strategies that enable them to invade tissues and spread within the host. Enterococcus faecalis is a leading cause of local and disseminated multidrug-resistant hospital infections, but the molecular mechanisms used by ... ...

Abstract	Bacterial pathogens have evolved strategies that enable them to invade tissues and spread within the host. Enterococcus faecalis is a leading cause of local and disseminated multidrug-resistant hospital infections, but the molecular mechanisms used by this non-motile bacterium to penetrate surfaces and translocate through tissues remain largely unexplored. Here we present experimental evidence indicating that E. faecalis generates exopolysaccharides containing β-1,6-linked poly-N-acetylglucosamine (polyGlcNAc) as a mechanism to successfully penetrate semisolid surfaces and translocate through human epithelial cell monolayers. Genetic screening and molecular analyses of mutant strains identified glnA, rpiA and epaX as genes critically required for optimal E. faecalis penetration and translocation. Mechanistically, GlnA and RpiA cooperated to generate uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) that was utilized by EpaX to synthesize polyGlcNAc-containing polymers. Notably, exogenous supplementation with polymeric N-acetylglucosamine (PNAG) restored surface penetration by E. faecalis mutants devoid of EpaX. Our study uncovers an unexpected mechanism whereby the RpiA-GlnA-EpaX metabolic axis enables production of polyGlcNAc-containing polysaccharides that endow E. faecalis with the ability to penetrate surfaces. Hence, targeting carbohydrate metabolism or inhibiting biosynthesis of polyGlcNAc-containing exopolymers may represent a new strategy to more effectively confront enterococcal infections in the clinic.
MeSH term(s)	Bacterial Proteins ; Enterococcus faecalis/metabolism ; Enterococcus faecalis/pathogenicity ; Extracellular Polymeric Substance Matrix/metabolism ; Extracellular Polymeric Substance Matrix/physiology ; Gram-Positive Bacterial Infections ; Humans ; Polysaccharides, Bacterial/metabolism ; Polysaccharides, Bacterial/physiology
Chemical Substances	Bacterial Proteins ; Polysaccharides, Bacterial ; polysaccharide intercellular adhesin
Language	English
Publishing date	2019-02-11
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1007571
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito