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  1. Article ; Online: Intravascular ultrasound to aid in the diagnosis and revision of an intra-aortic pedicle screw: illustrative case.

    Ehlers, Landon D / Opperman, Patrick J / Mordeson, Jack E / Thompson, Jonathan R / Surdell, Daniel L

    Journal of neurosurgery. Case lessons

    2023  Volume 6, Issue 7

    Abstract: Background: Pedicle screw impingement on vessel walls has the potential for complications due to pulsatile effects and wall erosion. Artifacts from spinal instrumentation create difficulty in accurately evaluating this interface. The authors present the ...

    Abstract Background: Pedicle screw impingement on vessel walls has the potential for complications due to pulsatile effects and wall erosion. Artifacts from spinal instrumentation create difficulty in accurately evaluating this interface. The authors present the first case of intravascular ultrasound (IVUS) used to characterize a pedicle screw breach into the aortic lumen.
    Observations: A 21-year-old female with surgically corrected scoliosis underwent computed tomography angiography (CTA) 3 years postoperatively, which revealed a pedicle screw within the thoracic aorta lumen. Metal artifact distorted the CTA images, which prompted the decision to use intraoperative IVUS. The IVUS confirmed the noninvasive imaging findings and guided final decisions regarding aortic endograft size and location during spine hardware revision.
    Lessons: For asymptomatic patients presenting with pedicle screws malpositioned in or near the aorta, treatment decisions revolve around the extent of vessel wall penetration. Intraluminal depth can be obscured by artifact on computed tomography or magnetic resonance imaging or inadequately evaluated by a transesophageal echocardiogram. In our intraoperative experience, IVUS confirmed the depth of vessel lumen violation by a single pedicle screw and no wall penetration by two additional screws of concern. This was useful in deciding on thoracic endovascular aortic repair graft size and landing zone and facilitated safe spinal instrumentation removal and revision.
    Language English
    Publishing date 2023-08-14
    Publishing country United States
    Document type Journal Article
    ISSN 2694-1902
    ISSN (online) 2694-1902
    DOI 10.3171/CASE23272
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Promiscuous dye binding by a light-up aptamer: application for label-free multi-wavelength biosensing.

    Connelly, Ryan P / Madalozzo, Pedro F / Mordeson, Jack E / Pratt, Andrew D / Gerasimova, Yulia V

    Chemical communications (Cambridge, England)

    2021  Volume 57, Issue 30, Page(s) 3672–3675

    Abstract: Light-up DNA aptamers are promising label-free signal-transducers for biosensing applications due to their high chemical stability and low synthetic cost. Herein, we demonstrate that a dapoxyl DNA aptamer DAP-10-42 can be converted into a sensor ... ...

    Abstract Light-up DNA aptamers are promising label-free signal-transducers for biosensing applications due to their high chemical stability and low synthetic cost. Herein, we demonstrate that a dapoxyl DNA aptamer DAP-10-42 can be converted into a sensor generating a fluorescence signal at different wavelengths in the range of 500-660 nm depending on the dye that is present. This results from the discovered promiscuity of DAP-10-42 in binding fluorogenic dyes including arylmethane dyes. We have designed a split DAP-10-42 aptasensor for the detection of a katG gene fragment from Mycobacterium tuberculosis with a point mutation causing isoniazid resistance. Efficient interrogation of the gene fragment after nucleic acid sequence-based amplification (NASBA) is achieved directly in a protein-containing NASBA sample. This report lays a foundation for the application of the DAP-10-42 aptamer as a versatile sensing platform.
    MeSH term(s) Aptamers, Nucleotide/chemistry ; Bacterial Proteins/analysis ; Bacterial Proteins/genetics ; Binding Sites ; Biosensing Techniques ; Catalase/analysis ; Catalase/genetics ; Fluorescent Dyes/chemistry ; Molecular Structure ; Mycobacterium tuberculosis/genetics ; Point Mutation
    Chemical Substances Aptamers, Nucleotide ; Bacterial Proteins ; Fluorescent Dyes ; Catalase (EC 1.11.1.6) ; katG protein, Mycobacterium tuberculosis (EC 1.11.1.6)
    Language English
    Publishing date 2021-03-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/d1cc00594d
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Citrullinated and malondialdehyde-acetaldehyde modified fibrinogen activates macrophages and promotes an aggressive synovial fibroblast phenotype in patients with rheumatoid arthritis.

    Aripova, Nozima / Duryee, Michael J / England, Bryant R / Hunter, Carlos D / Mordeson, Jack E / Ryan, Evan M / Daubach, Eric C / Romberger, Debra J / Thiele, Geoffrey M / Mikuls, Ted R

    Frontiers in immunology

    2023  Volume 14, Page(s) 1203548

    Abstract: Objective: Post-translational protein modifications with malondialdehyde-acetaldehyde (MAA) and citrulline (CIT) are implicated in the pathogenesis of rheumatoid arthritis (RA). Although precise mechanisms have not been elucidated, macrophage-fibroblast ...

    Abstract Objective: Post-translational protein modifications with malondialdehyde-acetaldehyde (MAA) and citrulline (CIT) are implicated in the pathogenesis of rheumatoid arthritis (RA). Although precise mechanisms have not been elucidated, macrophage-fibroblast interactions have been proposed to play a central role in the development and progression of RA. The purpose of our study was to evaluate the downstream effects of macrophage released soluble mediators, following stimulation with fibrinogen (FIB) modified antigens, on human fibroblast-like synoviocytes (HFLS).
    Methods: PMA-treated U-937 monocytes (Mϕ) and macrophage-differentiated peripheral blood mononuclear cells (MP) were stimulated with FIB, FIB-MAA, FIB-CIT, or FIB-MAA-CIT. HFLS-RA cells were stimulated directly with FIB antigens or with supernatants (SN) from macrophages (Mϕ-SN or MP-SN) stimulated with FIB antigens. Genes associated with an aggressive HFLS phenotype, extracellular matrix proteins, and activated signaling pathways were evaluated.
    Results: HFLS-RA cells treated with Mϕ-SN
    Conclusion: Together, these findings support the hypothesis that in response to MAA-modified and/or citrullinated fibrinogen, macrophages release soluble factors including PDGF-BB that induce fibroblast activation and promote an aggressive fibroblast phenotype. These cellular responses were most robust following macrophage activation with dually modified fibrinogen, compared to single modification alone, providing novel insights into the combined role of multiple post-translational protein modifications in the development of RA.
    MeSH term(s) Humans ; Fibrinogen ; Vimentin ; Becaplermin ; Collagen Type II ; Leukocytes, Mononuclear ; Proto-Oncogene Proteins c-akt ; Hemostatics ; Macrophages ; Fibroblasts ; Acetaldehyde ; Arthritis, Rheumatoid
    Chemical Substances Fibrinogen (9001-32-5) ; Vimentin ; Becaplermin (1B56C968OA) ; Collagen Type II ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Hemostatics ; Acetaldehyde (GO1N1ZPR3B)
    Language English
    Publishing date 2023-08-16
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2023.1203548
    Database MEDical Literature Analysis and Retrieval System OnLINE

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