Result 1 - 10 of total 59
2015
| Keywords | Cérebro ; Raiva ; Vacinas Antirrábicas |
|---|---|
| Publishing date | 2015-12-08T02:53:00Z |
| Document type | Book ; Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
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2015
| Keywords | Raiva ; Vacinas Antirrábicas ; Vacinação ; Brasil |
|---|---|
| Publishing date | 2015-12-08T02:50:25Z |
| Document type | Book ; Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
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2015
| Keywords | Raiva ; Vacinas Antirrábicas ; Brasil |
|---|---|
| Publishing date | 2015-12-08T03:10:18Z |
| Document type | Book ; Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
1999 Volume 75, Issue 5, Page(s) 373–376
Abstract: OBJECTIVE: To emphasize important aspects for the diagnosis and approach of patients with history of child abuse, related with the Munchausen Syndrome by proxy, and its variant, Hypochondriases by proxy. METHODS: The authors describe two cases followed ... ...
| Title translation | Hypochondriasis by proxy in children: report of two cases |
|---|---|
| Abstract | OBJECTIVE: To emphasize important aspects for the diagnosis and approach of patients with history of child abuse, related with the Munchausen Syndrome by proxy, and its variant, Hypochondriases by proxy. METHODS: The authors describe two cases followed in the Pediatric Service in a terciary hospital of Salvador, Bahia, in which they found aspects of the medical history and physical examination of these children and in the mothers' behavior compatible with Munchausen by proxy (Factitious disorder by proxy). RESULTS: In the two cases presented, we observed a standard in the clinical history, described as recurrent inpatient and outpatient medical support, through the last three years, without the identification of abnormal results. These facts do not decreased the certainty of the mothers about their children's disease (hypochondriases by proxy). Furthermore we observed cooperation on the part of the children by incorporating the role of the sick one, which made their school and social adaptation more difficult. CONCLUSIONS: The pediatrician must be aware of the diagnosis of Munchausen Syndrome by Proxy and its variation, hypochondriases by proxy, in those cases of unsuitable and recurrent disease, lack of symptoms and signs in the absence of parents, and diagnostic methods with meaningless results. When absolutely certain about diagnosis, the physician must establish the risk for the child (increased mortality in these cases) and suggest psychotherapy for the mother or perpetrator. |
| Language | Portuguese |
| Publishing date | 1999-09 |
| Publishing country | Brazil |
| Document type | Journal Article |
| ZDB-ID | 731324-x |
| ISSN | 1678-4782 ; 0021-7557 |
| ISSN (online) | 1678-4782 |
| ISSN | 0021-7557 |
| DOI | 10.2223/jped.333 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.B 1265: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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2012 Volume 59, Issue 6, Page(s) 379–388
Abstract: We report the initial characterization of a leptospiral isolate, Leptospira interrogans, serogroup Sejroe, serovar Hardjo, genotype Hardjoprajitno, strain Norma, and its relatedness with L. interrogans, serogroup Sejroe, serovar Hardjo, genotype ... ...
| Abstract | We report the initial characterization of a leptospiral isolate, Leptospira interrogans, serogroup Sejroe, serovar Hardjo, genotype Hardjoprajitno, strain Norma, and its relatedness with L. interrogans, serogroup Sejroe, serovar Hardjo, genotype Hardjoprajitno, strain Hardjo and Leptospira borgpetersenii, serogroup Sejroe, serovar Hardjo, genotype Hardjobovis, strain Sponselee. The Norma strain singled out during a leptospirosis outbreak in cattle immunized with antigens from the reference strain Hardjoprajitno (OMS). By applying a microscopic agglutination serological test (MAT) to cattle (n = 2966) with symptoms of leptospirosis between 2003 and 2007, more than 50% of sera were found positive for one of the following serotypes: Hardjoprajitno (31-21%), Hardjo Norma (46-40%), Hardjo hardjobovis (18-10%), Mini (8-4%) and Wolffi (7-4%). In immunization trials using six isolates plus Norma isolate, the remission of MAT in these isolates was observed following 6 months of the initial vaccination. To provide molecular ground for the high MAT Norma frequency found in these isolates, a DNA polymorphic analysis was conducted by comparing the Norma isolate with reference strains Hardjoprajitno and Sponselee. The polymorphic analysis in secY showed five base changes in Norma relative to Hardjoprajitno strain, corresponding to 98% identity, while Sponselee displayed 49 polymorphic sites relative to the Hardjoprajitno strain, representing 80% identity. The alignment of secY translated sequences shows no differences between Hardjoprajitno and Norma, and eight polymorphisms between genotype hardjoprajttno and strain Sponselee. Three-dimensional modelling located these variations within the loop region connecting helices 7 and 8 from secY which is less conserved. DNA sequencing of 23S ribosomal conserved fragment revealed a single polymorphism between Hardjoprajitno and Norma, and 13 polymorphisms between strains Sponselee, Hardjoprajitno and Norma. The differences between Hardjo and Norma were confirmed by low stringency single-specific primer polymerase chain reaction (LSSP-PCR) signature experiments with the primer G2, using as template the 285 bp fragment initially amplified with G1/G2 primers. |
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| MeSH term(s) | Agglutination Tests/veterinary ; Animals ; Antigens, Bacterial/immunology ; Base Sequence ; Brazil ; Cattle ; Cattle Diseases/microbiology ; Cattle Diseases/prevention & control ; DNA Primers/genetics ; DNA, Bacterial/genetics ; Female ; Genotype ; Leptospira interrogans/classification ; Leptospira interrogans/genetics ; Leptospira interrogans/immunology ; Leptospira interrogans/isolation & purification ; Leptospirosis/microbiology ; Leptospirosis/prevention & control ; Leptospirosis/veterinary ; Models, Molecular ; Molecular Sequence Data ; Phylogeny ; Polymorphism, Genetic/genetics ; Polymorphism, Restriction Fragment Length ; Pregnancy ; Sequence Alignment/veterinary ; Sequence Analysis, DNA/veterinary ; Species Specificity ; Vaccination/veterinary |
| Chemical Substances | Antigens, Bacterial ; DNA Primers ; DNA, Bacterial |
| Language | English |
| Publishing date | 2012-05-11 |
| Publishing country | Germany |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2271118-1 |
| ISSN | 1863-2378 ; 1863-1959 |
| ISSN (online) | 1863-2378 |
| ISSN | 1863-1959 |
| DOI | 10.1111/j.1863-2378.2012.01470.x |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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Zoonoses and public health. 2012 Sept., v. 59, no. 6
2012
Abstract: We report the initial characterization of a leptospiral isolate, Leptospira interrogans, serogroup Sejroe, serovar Hardjo, genotype Hardjoprajitno, strain Norma, and its relatedness with L. interrogans, serogroup Sejroe, serovar Hardjo, genotype ... ...
| Abstract | We report the initial characterization of a leptospiral isolate, Leptospira interrogans, serogroup Sejroe, serovar Hardjo, genotype Hardjoprajitno, strain Norma, and its relatedness with L. interrogans, serogroup Sejroe, serovar Hardjo, genotype Hardjoprajitno, strain Hardjo and Leptospira borgpetersenii, serogroup Sejroe, serovar Hardjo, genotype Hardjobovis, strain Sponselee. The Norma strain singled out during a leptospirosis outbreak in cattle immunized with antigens from the reference strain Hardjoprajitno (OMS). By applying a microscopic agglutination serological test (MAT) to cattle (n = 2966) with symptoms of leptospirosis between 2003 and 2007, more than 50% of sera were found positive for one of the following serotypes: Hardjoprajitno (31–21%), Hardjo Norma (46–40%), Hardjo hardjobovis (18–10%), Mini (8–4%) and Wolffi (7–4%). In immunization trials using six isolates plus Norma isolate, the remission of MAT in these isolates was observed following 6 months of the initial vaccination. To provide molecular ground for the high MAT Norma frequency found in these isolates, a DNA polymorphic analysis was conducted by comparing the Norma isolate with reference strains Hardjoprajitno and Sponselee. The polymorphic analysis in secY showed five base changes in Norma relative to Hardjoprajitno strain, corresponding to 98% identity, while Sponselee displayed 49 polymorphic sites relative to the Hardjoprajitno strain, representing 80% identity. The alignment of secY translated sequences shows no differences between Hardjoprajitno and Norma, and eight polymorphisms between genotype hardjoprajttno and strain Sponselee. Three‐dimensional modelling located these variations within the loop region connecting helices 7 and 8 from secY which is less conserved. DNA sequencing of 23S ribosomal conserved fragment revealed a single polymorphism between Hardjoprajitno and Norma, and 13 polymorphisms between strains Sponselee, Hardjoprajitno and Norma. The differences between Hardjo and Norma were confirmed by low stringency single‐specific primer polymerase chain reaction (LSSP‐PCR) signature experiments with the primer G2, using as template the 285 bp fragment initially amplified with G1/G2 primers. |
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| Keywords | DNA ; Leptospira borgpetersenii ; Leptospira interrogans serovar hardjo ; agglutination tests ; antigens ; cattle ; genotype ; leptospirosis ; models ; nucleotide sequences ; polymerase chain reaction ; remission ; sequence analysis ; serotypes ; vaccination ; Brazil |
| Language | English |
| Dates of publication | 2012-09 |
| Size | p. 379-388. |
| Publishing place | Blackwell Publishing Ltd |
| Document type | Article |
| ZDB-ID | 2271118-1 |
| ISSN | 1863-2378 ; 1863-1959 |
| ISSN (online) | 1863-2378 |
| ISSN | 1863-1959 |
| DOI | 10.1111/j.1863-2378.2012.01470.x |
| Database | NAL-Catalogue (AGRICOLA) |
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2015 Volume 3, Issue 6
Abstract: Leptospirosis is caused by pathogenic bacteria of the genus Leptospira spp. This neglected re-emergent disease has global distribution and relevance in veterinary production. Here, we report the whole-genome sequence and annotation of Leptospira ... ...
| Abstract | Leptospirosis is caused by pathogenic bacteria of the genus Leptospira spp. This neglected re-emergent disease has global distribution and relevance in veterinary production. Here, we report the whole-genome sequence and annotation of Leptospira interrogans serovar Hardjo subtype Hardjoprajitno strain Norma, isolated from cattle in a livestock leptospirosis outbreak in Brazil. |
|---|---|
| Language | English |
| Publishing date | 2015-11-05 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2704277-7 |
| ISSN | 2169-8287 |
| ISSN | 2169-8287 |
| DOI | 10.1128/genomeA.01302-15 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 54, Iss 2, Pp 117-
2002 Volume 126
Abstract: Com o objetivo de avaliar em uma região cárstica o tipo de refúgio, as espécies de morcegos, a população de animais domésticos, as associações interespecíficas nas coabitações com outros mamíferos silvestres suscetíveis à raiva e a eficácia da warfarina ... ...
| Abstract | Com o objetivo de avaliar em uma região cárstica o tipo de refúgio, as espécies de morcegos, a população de animais domésticos, as associações interespecíficas nas coabitações com outros mamíferos silvestres suscetíveis à raiva e a eficácia da warfarina aplicada no dorso do Desmodus rotundus rotundus foi realizada uma pesquisa de maio de 1998 a março de 2000, nos municípios de Cordisburgo e Curvelo, Minas Gerais. Em 49 refúgios vistoriados, 29 naturais e 20 artificiais, localizados em 14 propriedades, encontrou-se o Desmodus rotundus rotundus em 18 abrigos naturais. Destes, 17 eram cavernas formadas pela dissolução ou abatimento de rocha calcária, típica do carste, e um era túnel escavado na terra pela ação das águas de um rio. As características geomorfológicas e de localização espacial foram registradas com base nas coordenadas geográficas, obtidas com auxílio de um sensor geográfico de posição. Nesses abrigos foram capturados e identificados 1457 morcegos de 14 espécies, sendo 640 Glossophaga soricina (Pallas, 1766), 566 Desmodus rotundus (E. Geoffroy, 1810), 73 Anoura geoffroyi (Gray 1838), 58 Trachops cirrhosus (Spix, 1823), 38 Diphylla ecaudata ecaudata (Spix, 1823), 23 Platyhrrinus lineatus (E. Geoffroy, 1810), 16 Lasiurus ega (Gervais, 1856), 14 Carollia perspicillata (Linnaeus, 1758), 13 Phyllostomus hastatus hastatus (Pallas, 1767), 9 Artibeus lituratus (Olfers, 1818), 3 Mimmon bennettii (Gray, 1838), 2 Myotis nigricans (Schinz, 1821), 1 Eptesicus brasiliensis (Desmarest, 1819) e 1 Pygoderma bilabiatum (Wagner, 1843). Não se conseguiu isolar ou detectar o vírus rábico no cérebro de 25 hematófagos selecionados e em 52 de outras espécies. A maioria desses abrigos também era usada por pacas (Agouti paca Linnaues, 1766), capivaras (Hydrochaeris hydrochaeris, Linnaues, 1766), guaxinins (Procyon cancrivorus, G. Cuvier, 1798) e raposas (Lycalopex vetulus, Lund, 1842) que são suscetíveis à raiva. Em 546 Desmodus rotundus rotundus foi aplicada na região interescapular, aproximadamente um grama de uma pasta contendo warfarina dissolvida em vaselina, na razão de dois gramas para cada 100 gramas do produto. Observou-se redução significativa na incidência de mordeduras em bovinos e eqüídeos e na presença ou vestígios recentes de Desmodus rotundus rotundus em quatro abrigos dos 18 que estavam habitados no início do trabalho. Nas vistorias pós-tratamento dos vampiros com warfarina, em todos os abrigos, não se encontraram morcegos não hematófagos ou mamíferos mortos ou com sinais clínicos de intoxicação atribuíveis ao anticoagulante. |
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| Keywords | Morcego ; raiva ; carste ; abrigo ; combate ; warfarina ; Zoology ; QL1-991 ; Science ; Q ; DOAJ:Zoology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences ; Animal culture ; SF1-1100 ; Agriculture ; S ; DOAJ:Animal Sciences ; DOAJ:Agriculture and Food Sciences |
| Language | English |
| Publishing date | 2002-01-01T00:00:00Z |
| Publisher | Universidade Federal de Minas Gerais |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 54, Iss 2, Pp 221-
2002 Volume 222
Abstract: Aiming to understanding the goat industry in the State of Rio de Janeiro, from 1998-2000, this study was carried out in 48 goat farms, 72.9% of which with an area of less than eight hectares. Also, 62.5% of the farms had irregular topography, and 69% of ... ...
| Abstract | Aiming to understanding the goat industry in the State of Rio de Janeiro, from 1998-2000, this study was carried out in 48 goat farms, 72.9% of which with an area of less than eight hectares. Also, 62.5% of the farms had irregular topography, and 69% of them kept Saanen breed in a confined system (93.7%). Animals were housed between 16 to 24 hours/day in suspended wooden stalls, and 83.3% were dairy goats. |
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| Keywords | Caprino ; forma de exploração ; Rio de Janeiro ; Zoology ; QL1-991 ; Science ; Q ; DOAJ:Zoology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences ; Animal culture ; SF1-1100 ; Agriculture ; S ; DOAJ:Animal Sciences ; DOAJ:Agriculture and Food Sciences |
| Language | English |
| Publishing date | 2002-01-01T00:00:00Z |
| Publisher | Universidade Federal de Minas Gerais |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
Genetics and molecular research : GMR
2009 Volume 8, Issue 1, Page(s) 334–344
Abstract: Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissue- ... ...
| Abstract | Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissue-specific promoters that guarantee correct expression of transgenes would be necessary. We used inverse polymerase chain reaction to isolate a promoter sequence of the Mec1 gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in cassava storage roots. In silico analysis revealed putative cis-acting regulatory elements within this promoter sequence, including root-specific elements that may be required for its expression in vascular tissues. Transient expression experiments showed that the Mec1 promoter is functional, since this sequence was able to drive GUS expression in bean embryonic axes. Results from our computational analysis can serve as a guide for functional experiments to identify regions with tissue-specific Mec1 promoter activity. The DNA sequence that we identified is a new promoter that could be a candidate for genetic engineering of cassava roots. |
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| MeSH term(s) | Base Sequence ; Binding Sites ; DNA, Complementary/metabolism ; DNA, Plant/metabolism ; Genes, Plant ; Glutamic Acid/analysis ; Manihot/genetics ; Manihot/metabolism ; Molecular Sequence Data ; Plant Proteins/chemistry ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plant Roots/genetics ; Plant Roots/metabolism ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; Sequence Analysis, DNA |
| Chemical Substances | DNA, Complementary ; DNA, Plant ; Plant Proteins ; Glutamic Acid (3KX376GY7L) |
| Language | English |
| Publishing date | 2009-03-24 |
| Publishing country | Brazil |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2114039-X |
| ISSN | 1676-5680 ; 1676-5680 |
| ISSN (online) | 1676-5680 |
| ISSN | 1676-5680 |
| DOI | 10.4238/vol8-1gmr560 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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