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Article ; Online: Author Correction: Low testosterone levels and high estradiol to testosterone ratio are associated with hyperinflammatory state and mortality in hospitalized men with COVID-19.

Infante, M / Pieri, M / Lupisella, S / D'Amore, L / Bernardini, S / Fabbri, A / Iannetta, M / Andreoni, M / Morello, M

European review for medical and pharmacological sciences

2024 Volume 27, Issue 24, Page(s) 11672

Abstract: Correction to: Eur Rev Med Pharmacol Sci 2021; 25 (19): 5889-5903. DOI: 10.26355/eurrev_202110_26865. PMID: 34661247-published online on October 12, 2021. In the main text, D-dimer unit of measurement was mistakenly indicated as mg/dL rather than as ng/ ... ...

Abstract	Correction to: Eur Rev Med Pharmacol Sci 2021; 25 (19): 5889-5903. DOI: 10.26355/eurrev_202110_26865. PMID: 34661247-published online on October 12, 2021. In the main text, D-dimer unit of measurement was mistakenly indicated as mg/dL rather than as ng/mL. The sentence "With regard to markers of coagulation, non-survivors showed significantly higher median levels of D-dimer as compared to survivors: 1348 mg/dL 949.5 mg/dL, respectively (p=0.03)." in its correct form is the following: "With regard to markers of coagulation, non-survivors showed significantly higher median levels of D-dimer as compared to survivors: 1348 ng/mL vs. 949.5 ng/mL, respectively (p=0.03).". In the first column of Table III (third row), D-dimer unit of measurement was mistakenly indicated as mg/dL rather than as ng/mL. Correction: "D-dimer (ng/mL)". There are amendments to this paper. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/26865.
Language	English
Publishing date	2024-01-02
Publishing country	Italy
Document type	Journal Article ; Published Erratum
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_202312_34763
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Article ; Online: Clinical Benefits of the Socket Shield Technique Associated with Anatomical Transmucosal Implant Components: A Case Report

Morello, Marco / Poglio, Enrico / De Maria, Andrea / Bresciano, Mario

The International journal of periodontics & restorative dentistry

2022 Volume 43, Issue 3, Page(s) 345–352

Abstract: One of the key factors in determining the esthetic success of implant-supported prostheses in the esthetic zone is the preservation of the natural architecture of the peri-implant tissues. A case replacing two maxillary lateral incisors with reduced ... ...

Abstract	One of the key factors in determining the esthetic success of implant-supported prostheses in the esthetic zone is the preservation of the natural architecture of the peri-implant tissues. A case replacing two maxillary lateral incisors with reduced buccal cortical bone by means of implant-supported crowns was illustrated. The socket shield technique was adopted to conserve the tissue volume, guided surgery was used to manage the optimal implant insertion, and anatomically shaped transmucosal implant components were selected to shape peri-implant soft tissues with the ideal emergence profiles and allow for direct digital impressions without scan bodies. The association of immediate implantation, guided surgery, socket shield technique, anatomically shaped transmucosal implant components, and digital impressions without scan bodies was used to achieve a successful rehabilitation with healthy, stable, and anatomically shaped peri-implant tissues.
MeSH term(s)	Humans ; Immediate Dental Implant Loading/methods ; Dental Implants, Single-Tooth ; Tooth Socket/surgery ; Crowns ; Tooth Extraction/methods ; Esthetics, Dental
Language	English
Publishing date	2022-12-15
Publishing country	United States
Document type	Case Reports ; Journal Article
ISSN	1945-3388
ISSN (online)	1945-3388
DOI	10.11607/prd.5016
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Article: Urine Parameters in Patients with COVID-19 Infection.

Morello, Maria / Amoroso, Dominga / Losacco, Felicia / Viscovo, Marco / Pieri, Massimo / Bernardini, Sergio / Adorno, Gaspare

Life (Basel, Switzerland)

2023 Volume 13, Issue 8

Abstract: A urine test permits the measure of several urinary markers. This is a non-invasive method for early monitoring of potential kidney damage. In COVID-19 patients, alterations of urinary markers were observed. This review aims to evaluate the utility of ... ...

Abstract	A urine test permits the measure of several urinary markers. This is a non-invasive method for early monitoring of potential kidney damage. In COVID-19 patients, alterations of urinary markers were observed. This review aims to evaluate the utility of urinalysis in predicting the severity of COVID-19. A total of 68 articles obtained from PubMed studies reported that (i) the severity of disease was related to haematuria and proteinuria and that (ii) typical alterations of the urinary sediment were noticed in COVID-19-associated AKI patients. This review emphasizes that urinalysis and microscopic examination support clinicians in diagnosing and predicting COVID-19 severity.
Language	English
Publishing date	2023-07-28
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662250-6
ISSN	2075-1729
ISSN	2075-1729
DOI	10.3390/life13081640
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Article ; Online: Low testosterone levels and high estradiol to testosterone ratio are associated with hyperinflammatory state and mortality in hospitalized men with COVID-19.

Infante, M / Pieri, M / Lupisella, S / D'Amore, L / Bernardini, S / Fabbri, A / Iannetta, M / Andreoni, M / Morello, M

European review for medical and pharmacological sciences

2021 Volume 25, Issue 19, Page(s) 5889–5903

Abstract: Objective: Evidence supports a sex disparity in clinical outcomes of COVID-19 patients, with men exhibiting higher mortality rates compared to women. We aimed to test the correlation between serum levels of sex hormones [total testosterone, estradiol ( ... ...

Abstract	Objective: Evidence supports a sex disparity in clinical outcomes of COVID-19 patients, with men exhibiting higher mortality rates compared to women. We aimed to test the correlation between serum levels of sex hormones [total testosterone, estradiol (E2), estradiol to testosterone (E2/T) ratio, progesterone), prolactin and 25-hydroxyvitamin D [25(OH)D] and markers of inflammation, coagulation and sepsis at admission in hospitalized men with COVID-19. Patients and methods: We conducted an exploratory retrospective study including symptomatic men with confirmed SARS-CoV-2 infection who were consecutively admitted to our Institution between April 1 and May 31, 2020. Results: Patients were divided into survivors (n=20) and non-survivors (n=39). As compared to survivors, non-survivors showed significantly higher median neutrophil-to-lymphocyte ratio (NLR) values, D-dimer and procalcitonin (PCT) levels, along with significantly lower median 25(OH)D levels and total testosterone levels. Non-survivors exhibited significantly higher median values of E2/T ratio (a marker of aromatase activity). Spearman's correlation analysis revealed that total testosterone levels were significantly and inversely correlated with NLR, high-sensitivity C-reactive protein (hsCRP), interleukin-6, D-dimer and PCT. Conversely, E2/T ratio values were significantly and positively correlated with the aforementioned markers and with white blood cell (WBC) count. In a multivariate analysis performed by a logistic regression model after adjusting for major confounders (age, body mass index, hypertension and cardiovascular disease, diabetes mellitus and malignancy), total testosterone levels were significantly and inversely associated with risk of COVID-19-related in-hospital mortality. Conclusions: Low total testosterone levels and elevated E2/T ratio values at admission are associated with hyperinflammatory state in hospitalized men with COVID-19. Low total testosterone levels at admission represent an independent risk factor for in-hospital mortality in such patients. Therefore, total testosterone and E2/T ratio may serve as prognostic markers of disease severity in this population.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; COVID-19/blood ; COVID-19/mortality ; Estradiol/blood ; Fibrin Fibrinogen Degradation Products/analysis ; Hospital Mortality ; Hospitalization ; Humans ; Inflammation/blood ; Inflammation/etiology ; Leukocyte Count ; Lymphocyte Count ; Male ; Middle Aged ; Procalcitonin/blood ; Retrospective Studies ; Risk Factors ; Severity of Illness Index ; Survival Analysis ; Testosterone/blood ; Vitamin D/analogs & derivatives ; Vitamin D/blood
Chemical Substances	Fibrin Fibrinogen Degradation Products ; Procalcitonin ; fibrin fragment D ; Vitamin D (1406-16-2) ; Testosterone (3XMK78S47O) ; Estradiol (4TI98Z838E) ; 25-hydroxyvitamin D (A288AR3C9H)
Language	English
Publishing date	2021-10-17
Publishing country	Italy
Document type	Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_202110_26865
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Article: Bridging the Generational Digital Divide in the Healthcare Environment.

Lopez de Coca, Teresa / Moreno, Lucrecia / Alacreu, Mónica / Sebastian-Morello, Maria

Journal of personalized medicine

2022 Volume 12, Issue 8

Abstract: Increasing technological advances have generated a digital dependency in the population, resulting in a group of digitally excluded vulnerable people that lack basic digital skills. The aim of this study was to assess the digital divide in patients in ... ...

Abstract	Increasing technological advances have generated a digital dependency in the population, resulting in a group of digitally excluded vulnerable people that lack basic digital skills. The aim of this study was to assess the digital divide in patients in relation to the healthcare environment. We explored the extent and effects of the digital health divide by undertaking a systematic review of the academic literature and comparing our findings with the results of a cross-sectional in-person survey answered by 881 people at four community pharmacies. In terms of the sociodemographic profile of the patients, we collected data regarding their gender, age, education level, and location (periphery or urban). The parameters evaluated were use of the internet to search for health information, use of telemedicine, use of different medical/healthcare applications, understanding explanations given by physicians regarding health, and asking pharmacists for help about newly prescribed treatments. Moreover, 168 pharmacists answered an online survey about how often they helped patients to make health center appointments or to download their COVID-19 vaccination certificate. Gender did not influence these results, but age, education level, and population location did. Those with the lowest levels of education required more help to request a health center appointment. People with high education levels and those living in an urban environment more often searched the internet for information about treatments that were new to them. Finally, people living in periphery areas received more help from their pharmacists, 60% of which said they had helped patients to download their COVID-19 vaccination certificate, with 24% of them saying they helped patients with this on a daily basis.
Language	English
Publishing date	2022-07-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662248-8
ISSN	2075-4426
ISSN	2075-4426
DOI	10.3390/jpm12081214
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Article: Short-Term TERT Inhibition Impairs Cellular Proliferation via a Telomere Length-Independent Mechanism and Can Be Exploited as a Potential Anticancer Approach.

Amin, Aamir / Morello, Marzia / Petrara, Maria Raffaella / Rizzo, Beatrice / Argenton, Francesco / De Rossi, Anita / Giunco, Silvia

Cancers

2023 Volume 15, Issue 10

Abstract: Telomerase reverse transcriptase (TERT), the catalytic component of telomerase, may also contribute to carcinogenesis via telomere-length independent mechanisms. Our previous in vitro and in vivo studies demonstrated that short-term telomerase inhibition ...

Abstract	Telomerase reverse transcriptase (TERT), the catalytic component of telomerase, may also contribute to carcinogenesis via telomere-length independent mechanisms. Our previous in vitro and in vivo studies demonstrated that short-term telomerase inhibition by BIBR1532 impairs cell proliferation without affecting telomere length. Here, we show that the impaired cell cycle progression following short-term TERT inhibition by BIBR1532 in in vitro models of B-cell lymphoproliferative disorders, i.e., Epstein-Barr virus (EBV)-immortalized lymphoblastoid cell lines (LCLs), and B-cell malignancies, i.e., Burkitt's lymphoma (BL) cell lines, is characterized by a significant reduction in NF-κB p65 nuclear levels leading to the downregulation of its target gene MYC. MYC downregulation was associated with increased expression and nuclear localization of P21, thus promoting its cell cycle inhibitory function. Consistently, treatment with BIBR1532 in wild-type zebrafish embryos significantly decreased Myc and increased
Language	English
Publishing date	2023-05-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers15102673
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Article ; Online: Prognostic role and interaction of TERT promoter status, telomere length and MGMT promoter methylation in newly diagnosed IDH wild-type glioblastoma patients.

Giunco, S / Padovan, M / Angelini, C / Cavallin, F / Cerretti, G / Morello, M / Caccese, M / Rizzo, B / d'Avella, D / Puppa, A D / Chioffi, F / De Bonis, P / Zagonel, V / De Rossi, A / Lombardi, G

ESMO open

2023 Volume 8, Issue 3, Page(s) 101570

Abstract: Background: The clinical relevance of promoter mutations and single nucleotide polymorphism rs2853669 of telomerase reverse transcriptase (TERT) and telomere length in patients with isocitrate dehydrogenase (IDH) wild-type glioblastoma (GBM) patients ... ...

Abstract	Background: The clinical relevance of promoter mutations and single nucleotide polymorphism rs2853669 of telomerase reverse transcriptase (TERT) and telomere length in patients with isocitrate dehydrogenase (IDH) wild-type glioblastoma (GBM) patients remains unclear. Moreover, some studies speculated that TERT promoter status might influence the prognostic role of O6-methylguanine DNA methyltransferase (MGMT) promoter methylation in newly diagnosed GBM. We carried out a large study to investigate their clinical impact and their interaction in newly diagnosed GBM patients. Patients and methods: We included 273 newly diagnosed IDH wild-type GBM patients who started treatment at Veneto Institute of Oncology IOV - IRCCS (Padua, Italy) from December 2016 to January 2020. TERT promoter mutations (-124 C>T and -146 C>T) and SNP rs2853669 (-245 T>C), relative telomere length (RTL) and MGMT methylation status were retrospectively assessed in this prospective cohort of patients. Results: Median overall survival (OS) of 273 newly diagnosed IDH wild-type GBM patients was 15 months. TERT promoter was mutated in 80.2% of patients, and most had the rs2853669 single nucleotide polymorphism as T/T genotype (46.2%). Median RTL was 1.57 (interquartile range 1.13-2.32). MGMT promoter was methylated in 53.4% of cases. At multivariable analysis, RTL and TERT promoter mutations were not associated with OS or progression-free survival (PFS). Notably, patients C carrier of rs2853669 (C/C+C/T genotypes) showed a better PFS compared with those with the T/T genotype (hazard ratio 0.69, P = 0.007). In terms of OS and PFS, all interactions between MGMT, TERT and RTL and between TERT and rs2853669 genotype were not statistically significant. Conclusions: Our findings suggest the presence of the C variant allele at the rs2853669 of the TERT promoter as an attractive independent prognostic biomarker of disease progression in IDH wild-type GBM patients. RTL and TERT promoter mutational status were not correlated to survival regardless of MGMT methylation status.
MeSH term(s)	Humans ; Prognosis ; Glioblastoma/genetics ; Isocitrate Dehydrogenase/genetics ; Retrospective Studies ; Methylation ; Prospective Studies ; Brain Neoplasms/diagnosis ; Telomere ; Telomerase/genetics ; DNA Modification Methylases/genetics ; Tumor Suppressor Proteins/genetics ; DNA Repair Enzymes/genetics
Chemical Substances	Isocitrate Dehydrogenase (EC 1.1.1.41) ; TERT protein, human (EC 2.7.7.49) ; Telomerase (EC 2.7.7.49) ; MGMT protein, human (EC 2.1.1.63) ; DNA Modification Methylases (EC 2.1.1.-) ; Tumor Suppressor Proteins ; DNA Repair Enzymes (EC 6.5.1.-)
Language	English
Publishing date	2023-05-23
Publishing country	England
Document type	Journal Article
ISSN	2059-7029
ISSN (online)	2059-7029
DOI	10.1016/j.esmoop.2023.101570
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Article ; Online: SARS-CoV-2 and indoor/outdoor air samples: a methodological approach to have consistent and comparable results.

Robotto, Angelo / Quaglino, Paola / Lembo, David / Morello, Marcello / Brizio, Enrico / Bardi, Luisella / Civra, Andrea

Environmental research

2021 Volume 195, Page(s) 110847

Abstract: Since the beginning of coronavirus disease 2019 (COVID-19) pandemic, large attention has been focused on the relationship between SARS-CoV-2 diffusion and environment. As a matter of fact, clear evidence of the transmission of SARS-CoV-2 via respiratory ... ...

Abstract	Since the beginning of coronavirus disease 2019 (COVID-19) pandemic, large attention has been focused on the relationship between SARS-CoV-2 diffusion and environment. As a matter of fact, clear evidence of the transmission of SARS-CoV-2 via respiratory aerosol would be of primary importance; at the same time, checking the presence of SARS-CoV-2 in wastewater can be extremely useful to control the diffusion of the disease. Up to now, many studies report SARS-CoV-2 concentrations in indoor/outdoor air samples or water/wastewater samples that can differ by order of magnitude. Unfortunately, complete information about the scientific approach of many studies is still missing, relating to: samplers and sampling materials performances, recovery tests, measurement uncertainty, robustness, detection and quantification limits, infectivity of captured virus, virus degradation during sampling, influence of sample pre-treatments (included freezing) on results, effects of inhibitors, sample alterations due to manipulation, validation of methods and processes, quality assurance according to ISO/IEC 17025 requirements. Based on the first experiences focused on the presence of SARS-CoV-2 in environmental samples such as air quality filters or impingers collection solutions, the present study describes a coherent preliminary approach to SARS-CoV-2 indoor and outdoor air sampling in order to overcome the evident lack of standardization. Three aspects are highlighted here: the first solution to assure quality and consistency to air sampling relies on the development of recovery tests using standard materials and investigating sampling materials, sampling techniques, sampling durations, sample conservation and pre-treatments; secondly, in order to overcome the shortcomings of every single sampling technique, coupling different samplers in parallel sampling could be an efficient strategy to collect more information and make data more reliable; finally, with regards to airborne virus sampling, the results could be confirmed by simplified emission and dilution models.
MeSH term(s)	Aerosols ; COVID-19 ; Humans ; Pandemics ; SARS-CoV-2
Chemical Substances	Aerosols
Language	English
Publishing date	2021-02-06
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2021.110847
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Article ; Online: Author Correction: Contribution of vitamin D3 and thiols status to the outcome of COVID-19 disease in Italian pediatric and adult patients.

D'Alessandro, Annamaria / Ciavardelli, Domenico / Pastore, Anna / Lupisella, Santina / Cristofaro, Rosa Carmela / Di Felice, Giovina / Salierno, Roberta / Infante, Marco / De Stefano, Alberto / Onetti Muda, Andrea / Morello, Maria / Porzio, Ottavia

Scientific reports

2023 Volume 13, Issue 1, Page(s) 4378

Language	English
Publishing date	2023-03-16
Publishing country	England
Document type	Published Erratum
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-31323-2
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Article ; Online: Admission eGFR predicts in-hospital mortality independently of admission glycemia and C-peptide in patients with type 2 diabetes mellitus and COVID-19.

Infante, Marco / Pieri, Massimo / Lupisella, Santina / Mohamad, Ali / Bernardini, Sergio / Della-Morte, David / Fabbri, Andrea / De Stefano, Alberto / Iannetta, Marco / Ansaldo, Lorenzo / Crea, Angela / Andreoni, Massimo / Morello, Maria

Current medical research and opinion

2023 Volume 39, Issue 4, Page(s) 505–516

Abstract: Objective: Type 2 diabetes mellitus (T2DM) and impaired kidney function are associated with a higher risk of poor outcomes of coronavirus disease 2019 (COVID-19). We conducted a retrospective study in hospitalized T2DM patients with COVID-19 to assess ... ...

Abstract	Objective: Type 2 diabetes mellitus (T2DM) and impaired kidney function are associated with a higher risk of poor outcomes of coronavirus disease 2019 (COVID-19). We conducted a retrospective study in hospitalized T2DM patients with COVID-19 to assess the association between in-hospital mortality and admission values of different hematological/biochemical parameters, including estimated glomerular filtration rate (eGFR), plasma glucose and C-peptide (the latter serving as a marker of beta-cell function). Methods: The study included T2DM patients with confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection who were consecutively admitted to our Institution between 1 October 2020 and 1 April 2021. Results: Patients ( Conclusions: Admission eGFR and WBC count predict in-hospital COVID-19 mortality among T2DM patients, independently of traditional risk factors, APG and random plasma C-peptide. Hospitalized patients with COVID-19 and comorbid T2DM associated with impaired kidney function at admission should be considered at high risk for adverse outcomes and death.
MeSH term(s)	Humans ; COVID-19/complications ; SARS-CoV-2 ; Diabetes Mellitus, Type 2/complications ; C-Peptide ; Retrospective Studies ; Glomerular Filtration Rate ; Hospital Mortality ; Blood Glucose
Chemical Substances	C-Peptide ; Blood Glucose
Language	English
Publishing date	2023-02-22
Publishing country	England
Document type	Journal Article
ZDB-ID	80296-7
ISSN	1473-4877 ; 0300-7995
ISSN (online)	1473-4877
ISSN	0300-7995
DOI	10.1080/03007995.2023.2177380
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