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  1. Article ; Online: Evaluating Riboglow-FLIM probes for RNA sensing.

    Sarfraz, Nadia / Shafik, Luke K / Stickelman, Zachary R / Shankar, Uma / Moscoso, Emilia / Braselmann, Esther

    RSC chemical biology

    2024  Volume 5, Issue 2, Page(s) 109–116

    Abstract: We recently developed Riboglow-FLIM, where we genetically tag and track RNA molecules in live cells through measuring the fluorescence lifetime of a small molecule probe that binds the RNA tag. Here, we systematically and quantitatively evaluated key ... ...

    Abstract We recently developed Riboglow-FLIM, where we genetically tag and track RNA molecules in live cells through measuring the fluorescence lifetime of a small molecule probe that binds the RNA tag. Here, we systematically and quantitatively evaluated key elements of Riboglow-FLIM that may serve as the foundation for Riboglow-FLIM applications and further tool development efforts. Our investigation focused on measuring changes in fluorescence lifetime of representative Riboglow-FLIM probes with different linkers and fluorophores in different environments.
    Language English
    Publishing date 2024-01-08
    Publishing country England
    Document type Journal Article
    ISSN 2633-0679
    ISSN (online) 2633-0679
    DOI 10.1039/d3cb00197k
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM).

    Sarfraz, Nadia / Moscoso, Emilia / Oertel, Therese / Lee, Harrison J / Ranjit, Suman / Braselmann, Esther

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 867

    Abstract: Visualization of RNAs in live cells is critical to understand biology of RNA dynamics and function in the complex cellular environment. Detection of RNAs with a fluorescent marker frequently involves genetically fusing an RNA aptamer tag to the RNA of ... ...

    Abstract Visualization of RNAs in live cells is critical to understand biology of RNA dynamics and function in the complex cellular environment. Detection of RNAs with a fluorescent marker frequently involves genetically fusing an RNA aptamer tag to the RNA of interest, which binds to small molecules that are added to live cells and have fluorescent properties. Engineering efforts aim to improve performance and add versatile features. Current efforts focus on adding multiplexing capabilities to tag and visualize multiple RNAs simultaneously in the same cell. Here, we present the fluorescence lifetime-based platform Riboglow-FLIM. Our system requires a smaller tag and has superior cell contrast when compared with intensity-based detection. Because our RNA tags are derived from a large bacterial riboswitch sequence family, the riboswitch variants add versatility for using multiple tags simultaneously. Indeed, we demonstrate visualization of two RNAs simultaneously with orthogonal lifetime-based tags.
    MeSH term(s) Animals ; Fluorescence ; Riboswitch/genetics ; Fluorescent Dyes/metabolism ; RNA/genetics ; RNA/metabolism ; Microscopy, Fluorescence ; Bacteria/metabolism ; Mammals/metabolism
    Chemical Substances Riboswitch ; Fluorescent Dyes ; RNA (63231-63-0)
    Language English
    Publishing date 2023-02-16
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-36531-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Assessment of

    Martin, Katy A / Kovach, Kristofer / Moscoso, Erica / Carreiro, Elizabeth / Jesudoss Chelladurai, Jeba R J / Brewer, Matthew T

    Frontiers in veterinary science

    2023  Volume 10, Page(s) 1282274

    Abstract: ... The ... ...

    Abstract The protozoan
    Language English
    Publishing date 2023-11-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2023.1282274
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Establishing Riboglow-FLIM to visualize noncoding RNAs inside live zebrafish embryos.

    Sarfraz, Nadia / Lee, Harrison J / Rice, Morgan K / Moscoso, Emilia / Shafik, Luke K / Glasgow, Eric / Ranjit, Suman / Lambeck, Ben J / Braselmann, Esther

    Biophysical reports

    2023  Volume 3, Issue 4, Page(s) 100132

    Abstract: The central role of RNAs in health and disease calls for robust tools to visualize RNAs in living systems through fluorescence microscopy. Live zebrafish embryos are a popular system to investigate multicellular complexity as disease models. However, RNA ...

    Abstract The central role of RNAs in health and disease calls for robust tools to visualize RNAs in living systems through fluorescence microscopy. Live zebrafish embryos are a popular system to investigate multicellular complexity as disease models. However, RNA visualization approaches in whole organisms are notably underdeveloped. Here, we establish our RNA tagging and imaging platform Riboglow-FLIM for complex cellular imaging applications by systematically evaluating FLIM capabilities. We use adherent mammalian cells as models for RNA visualization. Additional complexity of analyzing RNAs in whole mammalian animals is achieved by injecting these cells into a zebrafish embryo system for cell-by-cell analysis in this model of multicellularity. We first evaluate all variable elements of Riboglow-FLIM quantitatively before assessing optimal use in whole animals. In this way, we demonstrate that a model noncoding RNA can be detected robustly and quantitatively inside live zebrafish embryos using a far-red Cy5-based variant of the Riboglow platform. We can clearly resolve cell-to-cell heterogeneity of different RNA populations by this methodology, promising applicability in diverse fields.
    Language English
    Publishing date 2023-09-26
    Publishing country United States
    Document type Journal Article
    ISSN 2667-0747
    ISSN (online) 2667-0747
    DOI 10.1016/j.bpr.2023.100132
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Stability in Aqueous Solution of a New Spray-Dried Hydrocolloid of High Andean Algae

    Choque-Quispe, David / Ligarda-Samanez, Carlos A / Choque-Quispe, Yudith / Froehner, Sandro / Solano-Reynoso, Aydeé M / Moscoso-Moscoso, Elibet / Carhuarupay-Molleda, Yakov Felipe / Peréz-Salcedo, Ronald

    Polymers

    2024  Volume 16, Issue 4

    Abstract: There is a growing emphasis on seeking stabilizing agents with minimal transformation, prioritizing environmentally friendly alternatives, and actively contributing to the principles of the circular economy. This research aimed to assess the stability of ...

    Abstract There is a growing emphasis on seeking stabilizing agents with minimal transformation, prioritizing environmentally friendly alternatives, and actively contributing to the principles of the circular economy. This research aimed to assess the stability of a novel spray-dried hydrocolloid from high Andean algae when introduced into an aqueous solution.
    Language English
    Publishing date 2024-02-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym16040537
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Effect of Germination on the Physicochemical Properties, Functional Groups, Content of Bioactive Compounds, and Antioxidant Capacity of Different Varieties of Quinoa (

    Ramos-Pacheco, Betsy S / Choque-Quispe, David / Ligarda-Samanez, Carlos A / Solano-Reynoso, Aydeé M / Palomino-Rincón, Henry / Choque-Quispe, Yudith / Peralta-Guevara, Diego E / Moscoso-Moscoso, Elibet / Aiquipa-Pillaca, Ángel S

    Foods (Basel, Switzerland)

    2024  Volume 13, Issue 3

    Abstract: Germination is an effective strategy to improve the nutritional and functional quality of Andean grains such as quinoa ( ...

    Abstract Germination is an effective strategy to improve the nutritional and functional quality of Andean grains such as quinoa (
    Language English
    Publishing date 2024-01-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods13030417
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Thesis ; Online: Seismic structure off- and onshore Maule, constraints for megathrust earthquakes in central Chile

    Moscoso, Eduardo

    2011  

    Abstract: During the last decades, the Chilean margin offshore Maule (34±S −36±S) had been reported as a highly locked and seismically quiet zone. The stress-accumulated state finished on the 27th of February 2010, when a megathrust earthquake (with Mw = 8.8) ... ...

    Abstract During the last decades, the Chilean margin offshore Maule (34±S −36±S) had been reported as a highly locked and seismically quiet zone. The stress-accumulated state finished on the 27th of February 2010, when a megathrust earthquake (with Mw = 8.8) ruptured » 400 km of the Nazca-South America plate boundary. Unfortunately, up to now little was known about the seismic structure offshore Maule. In the frame of the third phase of the project SFB 574 “Volatiles and Fluids in Subduction Zones” of the Christan-Albrechts University of Kiel, seismic data was analyzed in order to obtain detailed images of the deep structure of the margin and of the outer rise. Here are presented constraints on the forearc and the subduction zone structure of the rupture area derived from seismic refraction and wide-angle data. The results show a wedge shaped body » 40 km wide with typical sedimentary velocities interpreted as a frontal accretionary prism (FAP). Landward of the imaged FAP, the velocity model shows an abrupt velocity-contrast suggesting a lithological change, which is interpreted as the contact between the FAP and the paleo accretionary prism (backstop). The backstop location is coincident with the seaward limit of the aftershocks, defining the updip limit of the co-seismic rupture and the seismogenic zone. Furthermore, the seaward limit of the aftershocks coincides with the location of the shelf break in the entire earthquake rupture area (33.5±S−38.5±S), which is interpreted as the location of the backstop along the margin. Published seismic profiles at the northern and southern limit of the rupture area also show the presence of a strong horizontal velocity gradient imaging the seismic backstop at a distance of » 30 km from the deformation front. The seismic wide-angle reflections from the top of the subducting oceanic crust constrain the location of the plate boundary offshore, dipping » 10±. The projection of the epicenter of the Maule earthquake onto our derived interplate boundary yielded a hypocenter around 20 km ...
    Subject code 550
    Language English
    Publishing country de
    Document type Thesis ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Thesis ; Online: Seismic structure off- and onshore Maule, constraints for megathrust earthquakes in central Chile

    Moscoso, Eduardo

    2011  

    Abstract: During the last decades, the Chilean margin offshore Maule (34±S −36±S) had been reported as a highly locked and seismically quiet zone. The stress-accumulated state finished on the 27th of February 2010, when a megathrust earthquake (with Mw = 8.8) ... ...

    Abstract During the last decades, the Chilean margin offshore Maule (34±S −36±S) had been reported as a highly locked and seismically quiet zone. The stress-accumulated state finished on the 27th of February 2010, when a megathrust earthquake (with Mw = 8.8) ruptured » 400 km of the Nazca-South America plate boundary. Unfortunately, up to now little was known about the seismic structure offshore Maule. In the frame of the third phase of the project SFB 574 “Volatiles and Fluids in Subduction Zones” of the Christan-Albrechts University of Kiel, seismic data was analyzed in order to obtain detailed images of the deep structure of the margin and of the outer rise. Here are presented constraints on the forearc and the subduction zone structure of the rupture area derived from seismic refraction and wide-angle data. The results show a wedge shaped body » 40 km wide with typical sedimentary velocities interpreted as a frontal accretionary prism (FAP). Landward of the imaged FAP, the velocity model shows an abrupt velocity-contrast suggesting a lithological change, which is interpreted as the contact between the FAP and the paleo accretionary prism (backstop). The backstop location is coincident with the seaward limit of the aftershocks, defining the updip limit of the co-seismic rupture and the seismogenic zone. Furthermore, the seaward limit of the aftershocks coincides with the location of the shelf break in the entire earthquake rupture area (33.5±S−38.5±S), which is interpreted as the location of the backstop along the margin. Published seismic profiles at the northern and southern limit of the rupture area also show the presence of a strong horizontal velocity gradient imaging the seismic backstop at a distance of » 30 km from the deformation front. The seismic wide-angle reflections from the top of the subducting oceanic crust constrain the location of the plate boundary offshore, dipping » 10±. The projection of the epicenter of the Maule earthquake onto our derived interplate boundary yielded a hypocenter around 20 km ...
    Subject code 550
    Language English
    Publishing country de
    Document type Thesis ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Preliminary Assessment of Tara Gum as a Wall Material: Physicochemical, Structural, Thermal, and Rheological Analyses of Different Drying Methods.

    Moscoso-Moscoso, Elibet / Ligarda-Samanez, Carlos A / Choque-Quispe, David / Huamán-Carrión, Mary L / Arévalo-Quijano, José C / De la Cruz, Germán / Luciano-Alipio, Rober / Calsina Ponce, Wilber Cesar / Sucari-León, Reynaldo / Quispe-Quezada, Uriel R / Calderón Huamaní, Dante Fermín

    Polymers

    2024  Volume 16, Issue 6

    Abstract: Tara gum, a natural biopolymer extracted ... ...

    Abstract Tara gum, a natural biopolymer extracted from
    Language English
    Publishing date 2024-03-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym16060838
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  10. Article ; Online: Effect of Inlet Air Temperature and Quinoa Starch/Gum Arabic Ratio on Nanoencapsulation of Bioactive Compounds from Andean Potato Cultivars by Spray-Drying.

    Ligarda-Samanez, Carlos A / Choque-Quispe, David / Moscoso-Moscoso, Elibet / Pozo, Lizeth M Flores / Ramos-Pacheco, Betsy S / Palomino-Rincón, Henry / Gutiérrez, Rodrigo J Guzmán / Peralta-Guevara, Diego E

    Molecules (Basel, Switzerland)

    2023  Volume 28, Issue 23

    Abstract: Nanoencapsulation of native potato bioactive compounds by spray-drying improves their stability and bioavailability. The joint effect of the inlet temperature and the ratio of the encapsulant (quinoa starch/gum arabic) on the properties of the ... ...

    Abstract Nanoencapsulation of native potato bioactive compounds by spray-drying improves their stability and bioavailability. The joint effect of the inlet temperature and the ratio of the encapsulant (quinoa starch/gum arabic) on the properties of the nanocapsules is unknown. The purpose of this study was to determine the best conditions for the nanoencapsulation of these compounds. The effects of two inlet temperatures (96 and 116 °C) and two ratios of the encapsulant (15 and 25%
    MeSH term(s) Starch ; Antioxidants/chemistry ; Gum Arabic/chemistry ; Anthocyanins/analysis ; Temperature ; Solanum tuberosum ; Chenopodium quinoa ; Nanocapsules ; Bays ; Phenols/analysis
    Chemical Substances Starch (9005-25-8) ; Antioxidants ; Gum Arabic (9000-01-5) ; Anthocyanins ; Nanocapsules ; Phenols
    Language English
    Publishing date 2023-11-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules28237875
    Database MEDical Literature Analysis and Retrieval System OnLINE

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