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Article ; Online: Baseline Genotype Testing to Assess Drug Resistance Before Beginning HIV Treatment.

Ambrosioni, Juan / Mosquera, Mar / Miró, José M

2019 Volume 320, Issue 20, Page(s) 2153–2154

MeSH term(s)	Adult ; Anti-Retroviral Agents ; Drug Resistance, Viral/drug effects ; Genotype ; HIV/drug effects ; HIV Infections ; Humans
Chemical Substances	Anti-Retroviral Agents
Language	English
Publishing date	2019-01-08
Publishing country	United States
Document type	Letter ; Comment
ZDB-ID	2958-0
ISSN	1538-3598 ; 0254-9077 ; 0002-9955 ; 0098-7484
ISSN (online)	1538-3598
ISSN	0254-9077 ; 0002-9955 ; 0098-7484
DOI	10.1001/jama.2018.15926
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Article ; Online: Resistance Testing for Integrase Strand Transfer Inhibitors in Naive, Human Immunodeficiency Virus-infected Individuals.

Ambrosioni, Juan / Mosquera, Mar / Miró, José M

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

2018 Volume 68, Issue 11, Page(s) 1976–1977

MeSH term(s)	Antiviral Agents ; Drug Resistance ; HIV ; HIV Infections ; Humans ; Integrase Inhibitors ; Integrases
Chemical Substances	Antiviral Agents ; Integrase Inhibitors ; Integrases (EC 2.7.7.-)
Language	English
Publishing date	2018-12-06
Publishing country	United States
Document type	Letter ; Comment
ZDB-ID	1099781-7
ISSN	1537-6591 ; 1058-4838
ISSN (online)	1537-6591
ISSN	1058-4838
DOI	10.1093/cid/ciy1019
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Zs.A 1505: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Surveillance of SARS-CoV-2 virus circulation using Acute Respiratory Infections sentinel system of Catalonia (PIDIRAC) during the 2019-2020 season: A retrospective observational study.

Jané, Mireia / Martínez, Ana / Ciruela, Pilar / Mosquera, Mar / Martínez, Miguel J / Basile, Luca / Vidal, Mª José / Nogueras, Mª Mercè / de Molina, Patricia / Vila, Jordi / Marcos, Mª Angeles

PloS one

2022 Volume 17, Issue 3, Page(s) e0264949

Abstract: Background: In the context of COVID-19 pandemic in Catalonia (Spain), the present study analyses respiratory samples collected by the primary care network using Acute Respiratory Infections Sentinel Surveillance System (PIDIRAC) during the 2019-2020 ... ...

Abstract	Background: In the context of COVID-19 pandemic in Catalonia (Spain), the present study analyses respiratory samples collected by the primary care network using Acute Respiratory Infections Sentinel Surveillance System (PIDIRAC) during the 2019-2020 season to complement the pandemic surveillance system in place to detect SARS-CoV-2. The aim of the study is to describe whether SARS-CoV-2 was circulating before the first confirmed case was detected in Catalonia, on February 25th, 2020. Methods: The study sample was made up of all samples collected by the PIDIRAC primary care network as part of the Influenza and Acute Respiratory Infections (ARI) surveillance system activities. The study on respiratory virus included coronavirus using multiple RT-PCR assays. All positive samples for human coronavirus were subsequently typed for HKU1, OC43, NL63, 229E. Every respiratory sample was frozen at-80°C and retrospectively studied for SARS-CoV-2 detection. A descriptive study was performed, analysing significant differences among variables related to SARS-CoV- 2 cases comparing with rest of coronaviruses cases through a bivariate study with Chi-squared test and statistical significance at 95%. Results: Between October 2019 and April 2020, 878 respiratory samples from patients with acute respiratory infection or influenza syndrome obtained by PIDIRAC were analysed. 51.9% tested positive for influenza virus, 48.1% for other respiratory viruses. SARS-CoV-2 was present in 6 samples. The first positive SARS-CoV-2 case had symptom onset on 2 March 2020. These 6 cases were 3 men and 3 women, aged between 25 and 50 years old. 67% had risk factors, none had previous travel history nor presented viral coinfection. All of them recovered favourably. Conclusion: Sentinel Surveillance PIDIRAC enhances global epidemiological surveillance by allowing confirmation of viral circulation and describes the epidemiology of generalized community respiratory viruses' transmission in Catalonia. The system can provide an alert signal when identification of a virus is not achieved in order to take adequate preparedness measures.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; COVID-19/diagnosis ; COVID-19/epidemiology ; Child ; Child, Preschool ; Coronavirus/classification ; Coronavirus/genetics ; Coronavirus/isolation & purification ; Female ; Humans ; Infant ; Male ; Middle Aged ; Orthomyxoviridae/classification ; Orthomyxoviridae/genetics ; Orthomyxoviridae/isolation & purification ; Primary Health Care ; RNA, Viral/genetics ; Respiratory Tract Infections/virology ; Retrospective Studies ; Sentinel Surveillance ; Spain/epidemiology ; Young Adult
Chemical Substances	RNA, Viral
Language	English
Publishing date	2022-03-14
Publishing country	United States
Document type	Journal Article ; Observational Study
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0264949
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Article ; Online: Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity.

Santos Bravo, Marta / Berengua, Carla / Marín, Pilar / Esteban, Montserrat / Rodriguez, Cristina / Del Cuerpo, Margarita / Miró, Elisenda / Cuesta, Genoveva / Mosquera, Mar / Sánchez-Palomino, Sonsoles / Vila, Jordi / Rabella, Núria / Marcos, María Ángeles

Journal of clinical microbiology

2021 Volume 60, Issue 1, Page(s) e0160921

Abstract: Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) ... ...

Abstract	Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples (
MeSH term(s)	Biomarkers ; COVID-19 ; Humans ; RNA ; RNA, Viral/genetics ; Reverse Transcription ; SARS-CoV-2
Chemical Substances	Biomarkers ; RNA, Viral ; RNA (63231-63-0)
Language	English
Publishing date	2021-10-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.01609-21
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Article ; Online: Infection with the Omicron variant of SARS-CoV-2 is associated with less severe disease in hospitalized patients with COVID-19.

Aiello, Tommaso Francesco / Puerta-Alcalde, Pedro / Chumbita, Mariana / Monzó, Patricia / Lopera, Carlos / Hurtado, Juan Carlos / Meira, Fernanda / Mosquera, Mar / Santos, Marta / Fernandez-Pittol, Mariana / Mensa, Josep / Martínez, José Antonio / Soriano, Alex / Marcos, Ma Angeles / Garcia-Vidal, Carolina

The Journal of infection

2022 Volume 85, Issue 5, Page(s) e152–e154

MeSH term(s)	COVID-19 ; Cell Line ; Humans ; SARS-CoV-2
Language	English
Publishing date	2022-08-06
Publishing country	England
Document type	Letter ; Research Support, Non-U.S. Gov't ; Comment
ZDB-ID	424417-5
ISSN	1532-2742 ; 0163-4453
ISSN (online)	1532-2742
ISSN	0163-4453
DOI	10.1016/j.jinf.2022.07.029
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Zs.A 1501: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Cellular and humoral immune response after mRNA-1273 SARS-CoV-2 vaccine in liver and heart transplant recipients.

Herrera, Sabina / Colmenero, Jordi / Pascal, Mariona / Escobedo, Miguel / Castel, María A / Sole-González, Eduard / Palou, Eduard / Egri, Natalia / Ruiz, Pablo / Mosquera, Mar / Moreno, Asunción / Juan, Manel / Vilella, Anna / Soriano, Alex / Farrero, Marta / Bodro, Marta

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

2021 Volume 21, Issue 12, Page(s) 3971–3979

Abstract: Recently published studies have found an impaired immune response after SARS-CoV-2 vaccination in solid organ recipients. However, most of these studies have not assessed immune cellular responses in liver and heart transplant recipients. We ... ...

Abstract	Recently published studies have found an impaired immune response after SARS-CoV-2 vaccination in solid organ recipients. However, most of these studies have not assessed immune cellular responses in liver and heart transplant recipients. We prospectively studied heart and liver transplant recipients eligible for SARS-CoV-2 vaccination. Patients with past history of SARS-CoV-2 infection or SARS-CoV-2 detectable antibodies (IgM or IgG) were excluded. We assessed IgM/IgG antibodies and ELISpot against the S protein 4 weeks after receiving the second dose of the mRNA-1273 (Moderna) vaccine. Side effects, troponin I, liver tests and anti-HLA donor-specific antibodies (DSA) were also assessed. A total of 58 liver and 46 heart recipients received two doses of mRNA-1273 vaccine. Median time from transplantation to vaccination was 5.4 years (IQR 0.3-27). Sixty-four percent of the patients developed SARS-CoV-2 IgM/IgG antibodies and 79% S-ELISpot positivity. Ninety percent of recipients developed either humoral or cellular response (87% in heart recipients and 93% in liver recipients). Factors associated with vaccine unresponsiveness were hypogammaglobulinemia and vaccination during the first year after transplantation. Local and systemic side effects were mild or moderate, and none presented DSA or graft dysfunction after vaccination. Ninety percent of our patients did develop humoral or cellular responses to mRNA-1273 vaccine. Factors associated with vaccine unresponsiveness were hypogammaglobulinemia and vaccination during the first year after transplantation, highlighting the need to further protect these patients.
MeSH term(s)	Antibodies, Viral ; COVID-19 ; COVID-19 Vaccines ; Heart Transplantation ; Humans ; Immunity, Humoral ; Liver ; SARS-CoV-2 ; Transplant Recipients
Chemical Substances	Antibodies, Viral ; COVID-19 Vaccines
Language	English
Publishing date	2021-08-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2060594-8
ISSN	1600-6143 ; 1600-6135
ISSN (online)	1600-6143
ISSN	1600-6135
DOI	10.1111/ajt.16768
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Article ; Online: Prolonged viral replication in patients with hematologic malignancies hospitalized with COVID-19.

Haematologica

2022 Volume 107, Issue 7, Page(s) 1731–1735

MeSH term(s)	COVID-19 ; Hematologic Neoplasms/complications ; Hematologic Neoplasms/pathology ; Hospitalization ; Humans ; SARS-CoV-2 ; Virus Replication
Language	English
Publishing date	2022-07-01
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2333-4
ISSN	1592-8721 ; 0017-6567 ; 0390-6078
ISSN (online)	1592-8721
ISSN	0017-6567 ; 0390-6078
DOI	10.3324/haematol.2021.280407
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Article ; Online: Efficacy and safety of raltegravir plus lamivudine maintenance therapy.

Borjabad, Beatriz / Inciarte, Alexy / Chivite, Ivan / Gonzalez-Cordon, Ana / Mosquera, Mar / Hurtado, Carmen / Rovira, Cristina / Gonzalez, Tania / Sempere, Abiu / Torres, Berta / Calvo, Julia / De La Mora, Lorena / Martinez-Rebollar, Maria / Laguno, Montserrat / Foncillas, Alberto / Ambrosioni, Juan / Blanch, Jordi / Rodriguez, Ana / Solbes, Estela /

Llobet, Roger / Berrocal, Leire / Mallolas, Josep / Miro, Jose M / Alcami, Jose / Blanco, Jose L / Sanchez-Palomino, Sonsoles / De Lazzari, Elisa / Martinez, Esteban

The Journal of antimicrobial chemotherapy

2023 Volume 79, Issue 2, Page(s) 255–261

Abstract: Background: Decreasing medication burden with raltegravir plus lamivudine in virologically suppressed persons with HIV (PWH) maintained efficacy and was well tolerated at 24 weeks, but more comprehensive data over longer follow-up are required.: ... ...

Abstract	Background: Decreasing medication burden with raltegravir plus lamivudine in virologically suppressed persons with HIV (PWH) maintained efficacy and was well tolerated at 24 weeks, but more comprehensive data over longer follow-up are required. Methods: Prospective 48 week extension phase of the raltegravir plus lamivudine arm from a previous 24 week pilot randomized clinical trial in which virologically suppressed PWH were randomized 2:1 to switch to fixed-dose combination 150 mg lamivudine/300 mg raltegravir twice daily or to continue therapy. In this 48 week extension phase, raltegravir was dosed at 1200 mg/day and lamivudine 300 mg/day. Primary outcome was the proportion of PWH with treatment failure at Week 48. Secondary outcomes were changes in ultrasensitive plasma HIV RNA, HIV DNA in CD4 cells, serum IL-6, ultrasensitive C-reactive protein and sCD14, body composition, sleep quality, quality of life and adverse effects. Results: Between May 2018 and June 2019, 33 PWH were enrolled. One participant experienced virological failure without resistance mutations and re-achieved sustained virological suppression without therapy discontinuation, and two others discontinued therapy due to adverse effects. Treatment failure was 9% (95% CI 2%-24%) and 3% (95% CI 0%-17%) in the ITT and on-treatment populations. There were significant changes between baseline and Week 48 in serum cytokines but not in other secondary outcomes. Conclusions: Switching to raltegravir and lamivudine in PWH with virological suppression maintains efficacy and is well tolerated. This maintenance regimen might be a cost-effective option for PWH at risk of drug-drug interactions or needing to avoid specific toxicities of certain antiretroviral drugs or their negative impact on comorbidities.
MeSH term(s)	Humans ; Raltegravir Potassium/adverse effects ; Lamivudine/adverse effects ; HIV Infections/drug therapy ; Anti-HIV Agents/adverse effects ; Prospective Studies ; Quality of Life ; Drug Therapy, Combination ; Drug-Related Side Effects and Adverse Reactions ; Viral Load ; Treatment Outcome
Chemical Substances	Raltegravir Potassium (43Y000U234) ; Lamivudine (2T8Q726O95) ; Anti-HIV Agents
Language	English
Publishing date	2023-12-01
Publishing country	England
Document type	Randomized Controlled Trial ; Journal Article
ZDB-ID	191709-2
ISSN	1460-2091 ; 0305-7453
ISSN (online)	1460-2091
ISSN	0305-7453
DOI	10.1093/jac/dkad364
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Zs.A 1197: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Clinical characteristics, risk factors, and incidence of symptomatic coronavirus disease 2019 in a large cohort of adults living with HIV: a single-center, prospective observational study.

Inciarte, Alexy / Gonzalez-Cordon, Ana / Rojas, Jhon / Torres, Berta / de Lazzari, Elisa / de la Mora, Lorena / Martinez-Rebollar, Maria / Laguno, Montserrat / Callau, Pilar / Gonzalez-Navarro, Azucena / Leal, Lorna / Garcia, Felipe / Mallolas, Josep / Mosquera, Mar / Marcos, Maria A / Ambrosioni, Juan / Miro, Josep M / Martinez, Esteban / Blanco, Jose L

AIDS (London, England)

2020 Volume 34, Issue 12, Page(s) 1775–1780

Abstract: Background: It is unclear how characteristics, risk factors, and incidence of coronavirus disease 2019 (COVID-19) in people living with HIV (PLWH) differ from the general population.: Methods: Prospective observational single-center cohort study of ... ...

Abstract	Background: It is unclear how characteristics, risk factors, and incidence of coronavirus disease 2019 (COVID-19) in people living with HIV (PLWH) differ from the general population. Methods: Prospective observational single-center cohort study of adult PLWH reporting symptoms of COVID-19. We assessed clinical characteristics, risk factors for COVID-19 diagnosis and severity, and standardized incidence rate ratio for COVID-19 cases in PLWH cohort and in Barcelona. Results: From 1 March 2020 to 10 May 2020, 53 out of 5683 (0.9% confidence interval 0.7-1.2%) PLWH were diagnosed with COVID-19. Median age was 44 years, CD4 T cells were 618/μl and CD4/CD8 was 0.90. All but two individuals were virologically suppressed. Cough (87%) and fever (82%) were the most common symptoms. Twenty-six (49%) were admitted, six (14%) had severe disease, four (8%) required ICU admission, and two (4%) died. Several laboratory markers (lower O2 saturation and platelets, and higher leukocytes, creatinine, lactate dehydrogenase, C reactive protein, procalcitonin, and ferritin) were associated with COVID-19 severity. No HIV or antiretroviral-related factors were associated with COVID-19 diagnosis or severity. Standardized incidence rate ratios of confirmed or confirmed/probable COVID-19 in PLWH were 38% (95% confidence interval 27-52%, P < 0.0001) and 33% (95% confidence interval 21-50%, P < 0.0001), respectively relative to the general population. Conclusion: PLWH with COVID-19 did not differ from the rest of the HIV cohort. Clinical presentation, severity rate, and mortality were not dependent on any HIV-related or antiretroviral-related factor. COVID-19 standardized incidence rate was lower in PLWH than in the general population. These findings should be confirmed in larger multicenter cohort studies.
MeSH term(s)	Adult ; Anti-HIV Agents/therapeutic use ; Betacoronavirus ; CD4 Lymphocyte Count ; COVID-19 ; Coronavirus Infections/mortality ; Coronavirus Infections/physiopathology ; Female ; HIV Infections/complications ; HIV Infections/drug therapy ; Humans ; Incidence ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/mortality ; Pneumonia, Viral/physiopathology ; Prospective Studies ; Risk Factors ; SARS-CoV-2 ; Spain/epidemiology
Chemical Substances	Anti-HIV Agents
Keywords	covid19
Language	English
Publishing date	2020-08-10
Publishing country	England
Document type	Journal Article ; Observational Study
ZDB-ID	639076-6
ISSN	1473-5571 ; 0269-9370 ; 1350-2840
ISSN (online)	1473-5571
ISSN	0269-9370 ; 1350-2840
DOI	10.1097/QAD.0000000000002643
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Article ; Online: Assessment of RNA amplification by multiplex RT-PCR and IgM detection by indirect and capture ELISAs for the diagnosis of measles and rubella.

Sanz, Juan Carlos / Mosquera, Mar / Ramos, Belén / Ramírez, Rosa / de Ory, Fernando / Echevarria, Juan Emilio

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

2010 Volume 118, Issue 3, Page(s) 203–209

Abstract: The aim of the study was to compare RNA amplification using multiplex RT-PCR and IgM detection by means of indirect and capture ELISAs for the diagnosis of measles and rubella. A total of 229 cases of maculopapular rash with serum and throat swab samples ...

Abstract	The aim of the study was to compare RNA amplification using multiplex RT-PCR and IgM detection by means of indirect and capture ELISAs for the diagnosis of measles and rubella. A total of 229 cases of maculopapular rash with serum and throat swab samples were included. Specific serological IgM to measles and rubella was determined by Enzygnost (Siemens) and Platelia (Bio-Rad). Both viruses were researched using multiplex RT-PCR performed on throat samples. Criteria for inclusion of measles or rubella cases were a positive RT-PCR result for one virus and negative for the other; and/or a positive IgM result for one virus by both ELISAs and negative RT-PCR for the other virus. A total of 74 cases were classified as measles and 54 as rubella. In measles, sensitivity and specificity were 93.2% and 100% for RT-PCR, 97.3% and 98.1% for Enzygnost, and 90.5% and 95.5% for Platelia. For rubella, these values were 42.6% and 100% for RT-PCR, 100% and 97.1% for Enzygnost, and 94.4% and 98.3% for Platelia. Enzygnost and Platelia are useful techniques for detecting IgM against measles and rubella. RNA amplification by RT-PCR was both sensitive and specific for the diagnosis of measles; however, for rubella, the sensitivity of this technique must be improved.
MeSH term(s)	Adolescent ; Adult ; Antibodies, Viral/blood ; Child ; Child, Preschool ; Enzyme-Linked Immunosorbent Assay/methods ; Female ; Humans ; Immunoglobulin M/blood ; Infant ; Male ; Measles/diagnosis ; Measles virus/genetics ; Measles virus/immunology ; RNA, Viral/genetics ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Rubella/diagnosis ; Rubella virus/genetics ; Rubella virus/immunology
Chemical Substances	Antibodies, Viral ; Immunoglobulin M ; RNA, Viral
Language	English
Publishing date	2010-03
Publishing country	Denmark
Document type	Journal Article
ZDB-ID	93340-5
ISSN	1600-0463 ; 0903-4641
ISSN (online)	1600-0463
ISSN	0903-4641
DOI	10.1111/j.1600-0463.2009.02581.x
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