Article ; Online: Altered high-density lipoprotein composition and functions during severe COVID-19.
2021 Volume 11, Issue 1, Page(s) 2291
Abstract: Coronavirus disease 2019 (COVID-19) pandemic is affecting millions of patients worldwide. The consequences of initial exposure to SARS-CoV-2 go beyond pulmonary damage, with a particular impact on lipid metabolism. Decreased levels in HDL-C were reported ...
| Abstract | Coronavirus disease 2019 (COVID-19) pandemic is affecting millions of patients worldwide. The consequences of initial exposure to SARS-CoV-2 go beyond pulmonary damage, with a particular impact on lipid metabolism. Decreased levels in HDL-C were reported in COVID-19 patients. Since HDL particles display antioxidant, anti-inflammatory and potential anti-infectious properties, we aimed at characterizing HDL proteome and functionality during COVID-19 relative to healthy subjects. HDLs were isolated from plasma of 8 severe COVID-19 patients sampled at admission to intensive care unit (Day 1, D1) at D3 and D7, and from 16 sex- and age-matched healthy subjects. Proteomic analysis was performed by LC-MS/MS. The relative amounts of proteins identified in HDLs were compared between COVID-19 and controls. apolipoprotein A-I and paraoxonase 1 were confirmed by Western-blot analysis to be less abundant in COVID-19 versus controls, whereas serum amyloid A and alpha-1 antitrypsin were higher. HDLs from patients were less protective in endothelial cells stiumalted by TNFα (permeability, VE-cadherin disorganization and apoptosis). In these conditions, HDL inhibition of apoptosis was blunted in COVID-19 relative to controls. In conclusion, we show major changes in HDL proteome and decreased functionality in severe COVID-19 patients. |
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| MeSH term(s) | Apolipoprotein A-I/blood ; Aryldialkylphosphatase/analysis ; Aryldialkylphosphatase/blood ; COVID-19/blood ; COVID-19/epidemiology ; COVID-19/pathology ; COVID-19/virology ; Case-Control Studies ; Chromatography, Liquid/methods ; Endothelial Cells/pathology ; Female ; France/epidemiology ; Humans ; Lipoproteins, HDL/blood ; Male ; Middle Aged ; Pandemics ; Proteome/metabolism ; Proteomics/methods ; SARS-CoV-2/isolation & purification ; Serum Amyloid A Protein/metabolism ; Tandem Mass Spectrometry/methods ; Tumor Necrosis Factor-alpha/blood ; alpha 1-Antitrypsin/blood |
| Chemical Substances | Apolipoprotein A-I ; Lipoproteins, HDL ; Proteome ; Serum Amyloid A Protein ; Tumor Necrosis Factor-alpha ; alpha 1-Antitrypsin ; Aryldialkylphosphatase (EC 3.1.8.1) |
| Language | English |
| Publishing date | 2021-01-27 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2615211-3 |
| ISSN | 2045-2322 ; 2045-2322 |
| ISSN (online) | 2045-2322 |
| ISSN | 2045-2322 |
| DOI | 10.1038/s41598-021-81638-1 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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