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Article: Intracellular changes of a swine tracheal cell line infected with a Mycoplasma hyopneumoniae pathogenic strain

Leal Zimmer, Fernanda M.A / Barr, John R / Ferreira, Henrique Bunselmeyer / Moura, Hercules

Elsevier Ltd Microbial pathogenesis. 2019 Dec., v. 137

2019

Abstract: Mycoplasma hyopneumoniae is the etiological agent of enzootic pneumonia (EP), a widespread disease that causes major economic losses to the pig industry. The swine host response plays an important role in the outcome of M. hyopneumoniae infections. The ... ...

Abstract	Mycoplasma hyopneumoniae is the etiological agent of enzootic pneumonia (EP), a widespread disease that causes major economic losses to the pig industry. The swine host response plays an important role in the outcome of M. hyopneumoniae infections. The whole proteome of newborn pig trachea (NPTr) epithelial cells infected with the M. hyopneumoniae pathogenic strain 7448 was analyzed using an LC-MS/MS approach to shed light on intracellular processes triggered in response to the pathogen. Overall, 853 swine protein species were identified, 156 of which were differentially represented in response to M. hyopneumoniae 7448 infection in comparison with non-infected control cells. These differentially represented proteins were categorized by function. Fifty-seven of them were assigned to the immune system and/or response to stimulus functional subcategories. Comparative expression analysis of these immune-related proteins in NPTr cells infected with attenuated or non-pathogenic mycoplasmas (M. hyopneumoniae J strain and M. flocculare, respectively) revealed proteins whose abundance was altered only in response to the pathogenic M. hyopneumoniae 7448 strain. Among these proteins, calcium homeostasis and endoplasmic reticulum stress-related biomarkers were detected, providing evidence of molecular mechanisms that might lead to swine cell apoptosis.
Keywords	apoptosis ; biomarkers ; calcium ; cell lines ; endoplasmic reticulum ; enzootic diseases ; epithelial cells ; financial economics ; homeostasis ; immune system ; industry ; liquid chromatography ; mass spectrometry ; Mycoplasma flocculare ; Mycoplasma hyopneumoniae ; neonates ; pathogens ; pneumonia ; proteins ; proteome ; swine ; virulent strains
Language	English
Dates of publication	2019-12
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2019.103717
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Article ; Online: MALDI-TOF MS: An alternative approach for ribotyping Clostridioides difficile isolates in Brazil.

Carneiro, Leandro Gouveia / Pinto, Tatiana Castro Abreu / Moura, Hercules / Barr, John / Domingues, Regina Maria Cavalcanti Pilotto / Ferreira, Eliane de Oliveira

Anaerobe

2021 Volume 69, Page(s) 102351

Abstract: Clostridioides difficile is an important organism causing healthcare-associated infections. It has been documented that specific strains caused multiple outbreaks globally, and patients infected with those strains are more likely to develop severe C. ... ...

Abstract	Clostridioides difficile is an important organism causing healthcare-associated infections. It has been documented that specific strains caused multiple outbreaks globally, and patients infected with those strains are more likely to develop severe C. difficile infection (CDI). With the appearance of a variant strain, BI/NAP1 ribotype 027, responsible for several outbreaks and high mortality rates worldwide, the epidemiology of the CDI changed drastically in the United States, Europe, and some Latin American countries. Although the epidemic strain 027 was not yet detected in Brazil, there are ribotypes exclusively found in the country, such as, 131, 132, 133, 135, 142 and 143, which are responsible for outbreaks in Brazilian hospitals and nursing homes. Although PCR-ribotyping is the most used method in epidemiology studies of C. difficile, it is not available in Brazil. This study aimed to develop and validate an in-house database for detecting C. difficile ribotypes, usually involved in CDI in Brazilian hospitals, by using MALDI-TOF MS. A database with 19 different ribotypes, 13 with worldwide circulation and 6 Brazilian-restricted, was created based on 27 spectra readings of each ribotype. After BioNumerics analysis, neighbor-joining trees revealed that spectra were distributed in clusters according to ribotypes, showing that MALDI-TOF MS could discriminate all 19 ribotypes. Moreover, each ribotype showed a different profile with 42 biomarkers detected in total. Based on their intensity and occurrence, 13 biomarkers were chosen to compose ribotype-specific profiles, and in silico analysis showed that most of these biomarkers were uncharacterized proteins or well-conserved peptides, such as ribosomal proteins. A double-blind assessment using the 13 biomarkers correctly assigned the ribotype in 73% of the spectra analyzed, with 94%-100% of correct hits for 027 and for Brazilian ribotypes. Although further analyses are required, our results show that MALDI-TOF MS might be a reliable, fast and feasible alternative for epidemiological surveillance of C. difficile in Brazil.
MeSH term(s)	Bacterial Typing Techniques/methods ; Brazil ; Clostridioides difficile/genetics ; Clostridioides difficile/isolation & purification ; Clostridium Infections/diagnosis ; Feces/microbiology ; Genetic Variation ; Genotype ; Humans ; Ribotyping/methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Language	English
Publishing date	2021-02-20
Publishing country	England
Document type	Comparative Study ; Journal Article
ZDB-ID	1237621-8
ISSN	1095-8274 ; 1075-9964
ISSN (online)	1095-8274
ISSN	1075-9964
DOI	10.1016/j.anaerobe.2021.102351
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Article ; Online: Messenger RNA levels of the Polo-like kinase gene (PLK) correlate with cytokinesis in the Trypanosoma rangeli cell cycle.

Prestes, Elisa Beatriz / Stoco, Patrícia Hermes / de Moraes, Milene Höehr / Moura, Hércules / Grisard, Edmundo Carlos

Experimental parasitology

2019 Volume 204, Page(s) 107727

Abstract: Background: Trypanosoma rangeli is a protozoan parasite that is non-virulent to the mammalian host and is morphologically and genomically related to Trypanosoma cruzi, whose proliferation within the mammalian host is controversially discussed.: ... ...

Abstract	Background: Trypanosoma rangeli is a protozoan parasite that is non-virulent to the mammalian host and is morphologically and genomically related to Trypanosoma cruzi, whose proliferation within the mammalian host is controversially discussed. Objectives: We aimed to investigate the T. rangeli cell cycle in vitro and in vivo by characterizing the timespan of the parasite life cycle and by proposing a molecular marker to assess cytokinesis. Methodology: The morphological events and their timing during the cell cycle of T. rangeli epimastigotes were assessed using DNA staining, flagellum labelling and bromodeoxyuridine incorporation. Messenger RNA levels of four genes previously associated with the cell cycle of trypanosomatids (AUK1, PLK, MOB1 and TRACK) were evaluated in the different T. rangeli forms. Findings: T. rangeli epimastigotes completed the cell cycle in vitro in 20.8 h. PLK emerged as a potential molecular marker for cell division, as its mRNA levels were significantly increased in exponentially growing epimastigotes compared with growth-arrested parasites or in vitro-differentiated trypomastigotes. PLK expression in T. rangeli can be detected near the flagellum protrusion site, reinforcing its role in the cell cycle. Interestingly, T. rangeli bloodstream trypomastigotes exhibited very low mRNA levels of PLK and were almost entirely composed of parasites in G1 phase. Main conclusions: Our work is the first to describe the T. rangeli cell cycle in vitro and proposes that PLK mRNA levels could be a useful tool to investigate the T. rangeli ability to proliferate within the mammalian host bloodstream.
MeSH term(s)	Animals ; Bromodeoxyuridine/metabolism ; Cell Cycle/drug effects ; Cell Cycle/genetics ; Cell Cycle Proteins/genetics ; Cytokinesis/genetics ; Cytokinesis/physiology ; DNA, Protozoan/chemistry ; DNA, Protozoan/isolation & purification ; Flow Cytometry ; Fluorescent Antibody Technique ; Hydroxyurea/pharmacology ; Mice ; Mice, Inbred BALB C ; Nucleic Acid Synthesis Inhibitors/pharmacology ; Protein Serine-Threonine Kinases/genetics ; Proto-Oncogene Proteins/genetics ; RNA, Messenger/analysis ; RNA, Protozoan/genetics ; RNA, Protozoan/isolation & purification ; Time Factors ; Trypanosoma rangeli/cytology ; Trypanosoma rangeli/drug effects ; Trypanosoma rangeli/enzymology ; Trypanosoma rangeli/genetics ; Trypanosomiasis/parasitology ; Polo-Like Kinase 1
Chemical Substances	Cell Cycle Proteins ; DNA, Protozoan ; Nucleic Acid Synthesis Inhibitors ; Proto-Oncogene Proteins ; RNA, Messenger ; RNA, Protozoan ; Protein Serine-Threonine Kinases (EC 2.7.11.1) ; Bromodeoxyuridine (G34N38R2N1) ; Hydroxyurea (X6Q56QN5QC)
Language	English
Publishing date	2019-07-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	391089-1
ISSN	1090-2449 ; 0014-4894
ISSN (online)	1090-2449
ISSN	0014-4894
DOI	10.1016/j.exppara.2019.107727
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Article ; Online: Unraveling oxidative stress response in the cestode parasite Echinococcus granulosus.

Cancela, Martín / Paes, Jéssica A / Moura, Hercules / Barr, John R / Zaha, Arnaldo / Ferreira, Henrique B

Scientific reports

2019 Volume 9, Issue 1, Page(s) 15876

Abstract: Cystic hydatid disease (CHD) is a worldwide neglected zoonotic disease caused by Echinococcus granulosus. The parasite is well adapted to its host by producing protective molecules that modulate host immune response. An unexplored issue associated with ... ...

Abstract	Cystic hydatid disease (CHD) is a worldwide neglected zoonotic disease caused by Echinococcus granulosus. The parasite is well adapted to its host by producing protective molecules that modulate host immune response. An unexplored issue associated with the parasite's persistence in its host is how the organism can survive the oxidative stress resulting from parasite endogenous metabolism and host defenses. Here, we used hydrogen peroxide (H
MeSH term(s)	Animals ; Antioxidants/metabolism ; Caspase 3/metabolism ; Cathepsin D/metabolism ; Down-Regulation/drug effects ; Echinococcus granulosus/growth & development ; Echinococcus granulosus/metabolism ; Glutathione Transferase/metabolism ; Helminth Proteins/metabolism ; Hydrogen Peroxide/pharmacology ; Larva/metabolism ; Oxidative Stress/drug effects ; Proteasome Endopeptidase Complex/metabolism ; Proteomics/methods ; Up-Regulation/drug effects
Chemical Substances	Antioxidants ; Helminth Proteins ; Hydrogen Peroxide (BBX060AN9V) ; Glutathione Transferase (EC 2.5.1.18) ; Caspase 3 (EC 3.4.22.-) ; Cathepsin D (EC 3.4.23.5) ; Proteasome Endopeptidase Complex (EC 3.4.25.1)
Language	English
Publishing date	2019-11-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-019-52456-3
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Article ; Online: Intracellular changes of a swine tracheal cell line infected with a Mycoplasma hyopneumoniae pathogenic strain.

Leal Zimmer, Fernanda M A / Moura, Hercules / Barr, John R / Ferreira, Henrique Bunselmeyer

Microbial pathogenesis

2019 Volume 137, Page(s) 103717

Abstract	Mycoplasma hyopneumoniae is the etiological agent of enzootic pneumonia (EP), a widespread disease that causes major economic losses to the pig industry. The swine host response plays an important role in the outcome of M. hyopneumoniae infections. The whole proteome of newborn pig trachea (NPTr) epithelial cells infected with the M. hyopneumoniae pathogenic strain 7448 was analyzed using an LC-MS/MS approach to shed light on intracellular processes triggered in response to the pathogen. Overall, 853 swine protein species were identified, 156 of which were differentially represented in response to M. hyopneumoniae 7448 infection in comparison with non-infected control cells. These differentially represented proteins were categorized by function. Fifty-seven of them were assigned to the immune system and/or response to stimulus functional subcategories. Comparative expression analysis of these immune-related proteins in NPTr cells infected with attenuated or non-pathogenic mycoplasmas (M. hyopneumoniae J strain and M. flocculare, respectively) revealed proteins whose abundance was altered only in response to the pathogenic M. hyopneumoniae 7448 strain. Among these proteins, calcium homeostasis and endoplasmic reticulum stress-related biomarkers were detected, providing evidence of molecular mechanisms that might lead to swine cell apoptosis.
MeSH term(s)	Animals ; Apoptosis ; Cell Line ; Chromatography, Liquid ; Cytoplasm/immunology ; Cytoplasm/metabolism ; Cytoplasm/microbiology ; Gene Ontology ; Host-Pathogen Interactions/genetics ; Host-Pathogen Interactions/immunology ; Host-Pathogen Interactions/physiology ; Mycoplasma hyopneumoniae/pathogenicity ; Proteome/genetics ; Proteome/isolation & purification ; Swine ; Swine Diseases/immunology ; Swine Diseases/metabolism ; Swine Diseases/microbiology ; Tandem Mass Spectrometry ; Trachea/immunology ; Trachea/metabolism ; Trachea/microbiology
Chemical Substances	Proteome
Language	English
Publishing date	2019-09-05
Publishing country	England
Document type	Journal Article
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2019.103717
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Article ; Online: Differential responses to stress of two Mycoplasma hyopneumoniae strains.

Paes, Jéssica Andrade / Leal Zimmer, Fernanda M A / Moura, Hercules / Barr, John R / Ferreira, Henrique Bunselmeyer

Journal of proteomics

2019 Volume 199, Page(s) 67–76

Abstract: Mycoplasma hyopneumoniae is a respiratory pathogen, causing porcine enzootic pneumonia. To survive in the porcine respiratory tract, M. hyopneumoniae must cope with both oxidative and heat stress imposed by the host. To get insights into M. hyopneumoniae ...

Abstract	Mycoplasma hyopneumoniae is a respiratory pathogen, causing porcine enzootic pneumonia. To survive in the porcine respiratory tract, M. hyopneumoniae must cope with both oxidative and heat stress imposed by the host. To get insights into M. hyopneumoniae stress responses and pathogenicity mechanisms, the protein profiles of two M. hyopneumoniae strains, pathogenic 7448 strain and non-pathogenic strain J, were surveyed under oxidative (OS) or heat (HS) stress. M. hyopneumoniae strains were submitted to OS (0.5% hydrogen peroxide) or HS (temperature shifts to 42 °C) conditions and protein profiling was carried out by LC-MS/MS and label-free quantitative analyses. Data are available via ProteomeXchange with identifier PXD012742. Qualitative and quantitative differences involving 40-60 M. hyopneumoniae proteins were observed for both strains when comparing bacteria exposed to OS or HS to non-treated controls. However, no differences in abundance were found in proteins classically related to stress responses, as peroxidases and chaperones, suggesting that these proteins would be constitutively present in both strains in the tested conditions. Interestingly, under stress conditions, more virulence-related proteins were detected in M. hyopneumoniae 7448 differentially represented proteins than in M. hyopneumoniae J, suggesting that stress may trigger a differential response of the corresponding genes, shared by both strains.
MeSH term(s)	Animals ; Bacterial Proteins/analysis ; Bacterial Proteins/genetics ; Chromatography, Liquid ; Heat-Shock Response ; Mycoplasma hyopneumoniae/genetics ; Mycoplasma hyopneumoniae/pathogenicity ; Mycoplasma hyopneumoniae/physiology ; Oxidative Stress ; Proteome/analysis ; Proteome/genetics ; Species Specificity ; Stress, Physiological ; Swine ; Tandem Mass Spectrometry
Chemical Substances	Bacterial Proteins ; Proteome
Language	English
Publishing date	2019-03-09
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2019.03.006
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: MALDI-TOF MS: An alternative approach for ribotyping Clostridioides difficile isolates in Brazil

Carneiro, Leandro Gouveia / Pinto, Tatiana Castro Abreu / Moura, Hercules / Barr, John / Domingues, Regina Maria Cavalcanti Pilotto / Ferreira, Eliane de Oliveira

Anaerobe. 2021 June, v. 69

2021

Abstract	Clostridioides difficile is an important organism causing healthcare-associated infections. It has been documented that specific strains caused multiple outbreaks globally, and patients infected with those strains are more likely to develop severe C. difficile infection (CDI). With the appearance of a variant strain, BI/NAP1 ribotype 027, responsible for several outbreaks and high mortality rates worldwide, the epidemiology of the CDI changed drastically in the United States, Europe, and some Latin American countries. Although the epidemic strain 027 was not yet detected in Brazil, there are ribotypes exclusively found in the country, such as, 131, 132, 133, 135, 142 and 143, which are responsible for outbreaks in Brazilian hospitals and nursing homes. Although PCR-ribotyping is the most used method in epidemiology studies of C. difficile, it is not available in Brazil. This study aimed to develop and validate an in-house database for detecting C. difficile ribotypes, usually involved in CDI in Brazilian hospitals, by using MALDI-TOF MS. A database with 19 different ribotypes, 13 with worldwide circulation and 6 Brazilian-restricted, was created based on 27 spectra readings of each ribotype. After BioNumerics analysis, neighbor-joining trees revealed that spectra were distributed in clusters according to ribotypes, showing that MALDI-TOF MS could discriminate all 19 ribotypes. Moreover, each ribotype showed a different profile with 42 biomarkers detected in total. Based on their intensity and occurrence, 13 biomarkers were chosen to compose ribotype-specific profiles, and in silico analysis showed that most of these biomarkers were uncharacterized proteins or well-conserved peptides, such as ribosomal proteins. A double-blind assessment using the 13 biomarkers correctly assigned the ribotype in 73% of the spectra analyzed, with 94%–100% of correct hits for 027 and for Brazilian ribotypes. Although further analyses are required, our results show that MALDI-TOF MS might be a reliable, fast and feasible alternative for epidemiological surveillance of C. difficile in Brazil.
Keywords	Clostridium difficile ; biomarkers ; computer simulation ; databases ; epidemiology ; monitoring ; mortality ; peptides ; ribotypes ; Brazil ; Europe
Language	English
Dates of publication	2021-06
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1237621-8
ISSN	1075-9964
ISSN	1075-9964
DOI	10.1016/j.anaerobe.2021.102351
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Article: Messenger RNA levels of the Polo-like kinase gene (PLK) correlate with cytokinesis in the Trypanosoma rangeli cell cycle

Prestes, Elisa Beatriz / Stoco, Patrícia Hermes / de Moraes, Milene Höehr / Moura, Hércules / Grisard, Edmundo Carlos

Experimental parasitology. 2019 Sept., v. 204

2019

Abstract: Trypanosoma rangeli is a protozoan parasite that is non-virulent to the mammalian host and is morphologically and genomically related to Trypanosoma cruzi, whose proliferation within the mammalian host is controversially discussed.We aimed to investigate ...

Abstract	Trypanosoma rangeli is a protozoan parasite that is non-virulent to the mammalian host and is morphologically and genomically related to Trypanosoma cruzi, whose proliferation within the mammalian host is controversially discussed.We aimed to investigate the T. rangeli cell cycle in vitro and in vivo by characterizing the timespan of the parasite life cycle and by proposing a molecular marker to assess cytokinesis.The morphological events and their timing during the cell cycle of T. rangeli epimastigotes were assessed using DNA staining, flagellum labelling and bromodeoxyuridine incorporation. Messenger RNA levels of four genes previously associated with the cell cycle of trypanosomatids (AUK1, PLK, MOB1 and TRACK) were evaluated in the different T. rangeli forms.T. rangeli epimastigotes completed the cell cycle in vitro in 20.8 h. PLK emerged as a potential molecular marker for cell division, as its mRNA levels were significantly increased in exponentially growing epimastigotes compared with growth-arrested parasites or in vitro-differentiated trypomastigotes. PLK expression in T. rangeli can be detected near the flagellum protrusion site, reinforcing its role in the cell cycle. Interestingly, T. rangeli bloodstream trypomastigotes exhibited very low mRNA levels of PLK and were almost entirely composed of parasites in G1 phase.Our work is the first to describe the T. rangeli cell cycle in vitro and proposes that PLK mRNA levels could be a useful tool to investigate the T. rangeli ability to proliferate within the mammalian host bloodstream.
Keywords	DNA ; Trypanosoma cruzi ; Trypanosoma rangeli ; blood flow ; cytokinesis ; enzymes ; epimastigotes ; flagellum ; genes ; genetic markers ; mammals ; messenger RNA ; parasites ; staining ; trypomastigotes
Language	English
Dates of publication	2019-09
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	391089-1
ISSN	1090-2449 ; 0014-4894
ISSN (online)	1090-2449
ISSN	0014-4894
DOI	10.1016/j.exppara.2019.107727
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Differential secretome profiling of a swine tracheal cell line infected with mycoplasmas of the swine respiratory tract.

Leal Zimmer, Fernanda Munhoz Dos Anjos / Paludo, Gabriela Prado / Moura, Hercules / Barr, John R / Ferreira, Henrique Bunselmeyer

Journal of proteomics

2018 Volume 192, Page(s) 147–159

Abstract: Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetically similar. However, M. hyopneumoniae causes porcine enzootic pneumonia, while M. flocculare is a commensal bacterium. M. hyopneumoniae and M. flocculare do not penetrate their host cells, ... ...

Abstract	Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetically similar. However, M. hyopneumoniae causes porcine enzootic pneumonia, while M. flocculare is a commensal bacterium. M. hyopneumoniae and M. flocculare do not penetrate their host cells, and secreted proteins are important for bacterium-host interplay. Thus, the secretomes of a swine trachea cell line (NPTr) infected with M. hyopneumoniae 7448 (a pathogenic strain), M. hyopneumoniae J (a non-pathogenic strain) and M. flocculare were compared to shed light in bacterium-host interactions. Medium from the cultures was collected, and secreted proteins were identified by a LC-MS/MS. Overall numbers of identified host and bacterial proteins were, respectively, 488 and 58, for NPTr/M. hyopneumoniae 7448; 371 and 67, for NPTr/M. hyopneumoniae J; and 203 and 81, for NPTr/M. flocculare. The swine cells revealed different secretion profiles in response to the infection with each M. hyopneumoniae strain or with M. flocculare. DAMPs and extracellular proteasome proteins, secreted in response to cell injury and death, were secreted by NPTr cells infected with M. hyopneumoniae 7448. All three mycoplasmas secreted virulence factors during NPTr infection, but M. hyopneumoniae 7448 secreted higher number of adhesins and hypothetical proteins, that may be related with pathogenicity. SIGNIFICANCE: The enzootic pneumonia caused by mycoplasmas of swine respiratory tract has economic loss consequences in pig industry due to antibiotic costs and pig weight loss. However, some genetically similar mycoplasmas are pathogenic while others, such as Mycoplasma hyopneumoniae and Mycoplasma flocculare, are non-pathogenic. Here, we conducted an infection assay between swine cells and pathogenic and non-pathogenic mycoplasmas to decipher secreted proteins during host-pathogen interaction. Mycoplasma response to cell infection was also observed. Our study provided new insights on secretion profile of swine cells in response to the infection with pathogenic and non-pathogenic mycoplasmas. It was possible to observe that pathogenic M. hyopneumoniae 7448 secreted known virulence factors and swine cells responded by inducing cell death. Otherwise, M. hyopneumoniae J and M. flocculare, non-pathogenic mycoplasmas, secreted a different profile of virulence factors in response to swine cells. Consequently, swine cells altered their secretome profile, but the changes were not sufficient to cause disease.
MeSH term(s)	Animals ; Bacterial Proteins/metabolism ; Cell Line ; Mycoplasma/metabolism ; Mycoplasma hyopneumoniae/metabolism ; Pneumonia of Swine, Mycoplasmal/metabolism ; Pneumonia of Swine, Mycoplasmal/microbiology ; Proteome/metabolism ; Swine/microbiology ; Trachea/microbiology
Chemical Substances	Bacterial Proteins ; Proteome
Language	English
Publishing date	2018-08-31
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2018.08.018
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Govinda s. Visvesvara: a tribute.

Kaneshiro, Edna S / Marciano-Cabral, Francine / Moura, Hercules

The Journal of eukaryotic microbiology

2014 Volume 62, Issue 1, Page(s) 1–2

MeSH term(s)	Animals ; History, 20th Century ; History, 21st Century ; Humans ; India ; Microbiology/history ; Parasitology/history ; United States
Language	English
Publishing date	2014-08-14
Publishing country	United States
Document type	Biography ; Festschrift ; Historical Article ; Portrait
ZDB-ID	1147218-2
ISSN	1550-7408 ; 1066-5234
ISSN (online)	1550-7408
ISSN	1066-5234
DOI	10.1111/jeu.12143
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