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  1. Article ; Online: Full confluency, serum starvation, and roscovitine for inducing arrest in the G

    Rodrigues, Luanna Lorenna Vieira / Moura, Yasmin Beatriz França / Viana, João Vitor da Silva / de Oliveira, Lhara Ricarliany Medeiros / Praxedes, Érika Almeida / Vieira, José de Brito / Sales, Sarah Leyenne Alves / Silva, Herlon Victor Rodrigues / Luciano, Maria Claudia Dos Santos / Pessoa, Claudia / Pereira, Alexsandra Fernandes

    Animal reproduction

    2023  Volume 20, Issue 1, Page(s) e20230017

    Abstract: The puma population is constantly decreasing, and cloning by somatic cell nuclear transfer can be used to conserve the species. One of the factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We ...

    Abstract The puma population is constantly decreasing, and cloning by somatic cell nuclear transfer can be used to conserve the species. One of the factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We evaluated the effects of full confluency (~100%), serum starvation (0.5% serum), and roscovitine (15 µM) treatments on the cell cycle synchronization in G
    Language English
    Publishing date 2023-04-21
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 2227229-X
    ISSN 1984-3143 ; 1984-3143
    ISSN (online) 1984-3143
    ISSN 1984-3143
    DOI 10.1590/1984-3143-AR2023-0017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Use of a product based on wood vinegar of Eucalyptus clone I144 used in the control of bovine mastitis.

    da Silva, Bismark Alves / Feijó, Francisco Marlon Carneiro / Alves, Nilza Dutra / Pimenta, Alexandre Santos / Benicio, Leon Denner Moreira / da Silva Júnior, Enilson Claudio / Santos, Caio Sérgio / Pereira, Alexsandra Fernandes / Moura, Yasmin Beatriz França / Gama, Gil Sander Prospero / Neto, Evilasio de Souza Lima

    Veterinary microbiology

    2023  Volume 279, Page(s) 109670

    Abstract: Bovine mastitis is one of the most frequent diseases in dairy cattle worldwide. The use of antiseptics in milking, if properly used, can lead to a reduction in potentially pathogenic microorganisms and their transmission between herds. Several medicinal ... ...

    Abstract Bovine mastitis is one of the most frequent diseases in dairy cattle worldwide. The use of antiseptics in milking, if properly used, can lead to a reduction in potentially pathogenic microorganisms and their transmission between herds. Several medicinal plants have antiseptic potential, including eucalyptus (Eucalyptus spp.). Therefore, the objective of this study was to evaluate the effectiveness of wood vinegar from Eucalyptus urograndis clone GG I144 (EU) as an antiseptic in vitro and in vivo; in addition, to its cytotoxicity and antimicrobial resistance. Fifteen bovines were used, lactating females 3-6 years of age and divided into three groups of five animals each. The wood vinegar was placed in the teats of the animal for 28 days and collections of cellular debris were performed every 7 days. At the Veterinary Microbiology Laboratory (LAMIV) of UFERSA, the samples were processed and serial dilution was performed in Petri plates with plate count agar (PCA) at 37 °C. Cytotoxicity was verified based on morphological alterations and metabolic activity. Morphological changes were not observed in all cells incubated with 1 % pyroligneous extract. The in vitro data demonstrated antimicrobial activity against S. aureus, S. agalactiae, Salmonella, E. coli and P. aeruginosa. The bacteria of the genus Staphylococcus and Corynebacterium were resistant to penicillin (PEN), rifampicin (RIF), nitrofurantoin (NIT), erythromycin (ERI), and ciprofloxacin (CIP). The extract was used in vivo in the post-dipping of dairy cows, which reduced the microbiological load present in the mammary glands from 4.74 to 2.54 CFU, indicating its future use as an antiseptic.
    MeSH term(s) Female ; Animals ; Cattle ; Anti-Bacterial Agents/therapeutic use ; Staphylococcus aureus ; Eucalyptus ; Lactation ; Escherichia coli ; Mastitis, Bovine/microbiology ; Anti-Infective Agents, Local ; Milk/microbiology ; Dairying ; Cattle Diseases/drug therapy
    Chemical Substances Anti-Bacterial Agents ; wood vinegar (8030-97-5) ; Anti-Infective Agents, Local
    Language English
    Publishing date 2023-01-27
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2023.109670
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Efficiency of Pyroligneous Extract from

    Feijo, Francisco Marlon Carneiro / Fernandes, Fernando da Costa / Alves, Nilza Dutra / Pimenta, Alexandre Santos / Santos, Caio Sergio / Rodrigues, Gardenia Silvana de Oliveira / Pereira, Alexsandra Fernandes / Benicio, Leon Denner Moreira / Moura, Yasmin Beatriz França

    Animals : an open access journal from MDPI

    2022  Volume 12, Issue 18

    Abstract: Pyroligneous extract ... ...

    Abstract Pyroligneous extract of
    Language English
    Publishing date 2022-09-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani12182325
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Efficiency of Pyroligneous Extract from Jurema Preta (Mimosa tenuiflora [Willd.] Poiret) as an Antiseptic in Cats (Felis catus) Subjected to Ovariosalpingohysterectomy

    Feijo, Francisco Marlon Carneiro / Fernandes, Fernando da Costa / Alves, Nilza Dutra / Pimenta, Alexandre Santos / Santos, Caio Sergio / Rodrigues, Gardenia Silvana de Oliveira / Pereira, Alexsandra Fernandes / Benicio, Leon Denner Moreira / Moura, Yasmin Beatriz França

    Animals. 2022 Sept. 07, v. 12, no. 18

    2022  

    Abstract: Pyroligneous extract of Jurema preta (Mimosa tenuiflora [Willd.] Poiret) was evaluated for its efficacy as a cutaneous antiseptic in cats (Felis catus) that were subjected to ovariosalpingohysterectomy. For this purpose, 30 cats without a defined breed ... ...

    Abstract Pyroligneous extract of Jurema preta (Mimosa tenuiflora [Willd.] Poiret) was evaluated for its efficacy as a cutaneous antiseptic in cats (Felis catus) that were subjected to ovariosalpingohysterectomy. For this purpose, 30 cats without a defined breed were sterilized and divided into two groups. The first group was the positive control, treated with 0.5% chlorhexidine-alcohol solution, and the second group was treated with 20% pyroligneous extract of M. tenuiflora. Regardless of age and sex, all animals had visible healing at similar times. A significant reduction in bacterial growth was observed in animals treated with the extract, and no cytotoxicity was observed in the feline epithelial cells. In addition, surgical wounds of cats treated with M. tenuiflora extract exhibited improved healing. On agar plates, treatment with both chlorhexidine and M. tenuiflora extract resulted in the inhibition zones for all bacterial strains isolated from surgical wounds. Therefore, M. tenuiflora extract is demonstrated to have antiseptic effects on the surgical wounds of cats undergoing ovariosalpingohysterectomy.
    Keywords Mimosa tenuiflora ; agar ; antiseptics ; bacterial growth ; cats ; chlorhexidine ; cytotoxicity ; epithelium
    Language English
    Dates of publication 2022-0907
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani12182325
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Use of a product based on wood vinegar of Eucalyptus clone I144 used in the control of bovine mastitis

    da Silva, Bismark Alves / Feijó, Francisco Marlon Carneiro / Alves, Nilza Dutra / Pimenta, Alexandre Santos / Benicio, Leon Denner Moreira / da Silva Júnior, Enilson Claudio / Santos, Caio Sérgio / Pereira, Alexsandra Fernandes / Moura, Yasmin Beatriz França / Gama, Gil Sander Prospero / Neto, Evilasio de Souza Lima

    Veterinary Microbiology. 2023 Apr., v. 279 p.109670-

    2023  

    Abstract: Bovine mastitis is one of the most frequent diseases in dairy cattle worldwide. The use of antiseptics in milking, if properly used, can lead to a reduction in potentially pathogenic microorganisms and their transmission between herds. Several medicinal ... ...

    Abstract Bovine mastitis is one of the most frequent diseases in dairy cattle worldwide. The use of antiseptics in milking, if properly used, can lead to a reduction in potentially pathogenic microorganisms and their transmission between herds. Several medicinal plants have antiseptic potential, including eucalyptus (Eucalyptus spp.). Therefore, the objective of this study was to evaluate the effectiveness of wood vinegar from Eucalyptus urograndis clone GG I144 (EU) as an antiseptic in vitro and in vivo; in addition, to its cytotoxicity and antimicrobial resistance. Fifteen bovines were used, lactating females 3–6 years of age and divided into three groups of five animals each. The wood vinegar was placed in the teats of the animal for 28 days and collections of cellular debris were performed every 7 days. At the Veterinary Microbiology Laboratory (LAMIV) of UFERSA, the samples were processed and serial dilution was performed in Petri plates with plate count agar (PCA) at 37 °C. Cytotoxicity was verified based on morphological alterations and metabolic activity. Morphological changes were not observed in all cells incubated with 1 % pyroligneous extract. The in vitro data demonstrated antimicrobial activity against S. aureus, S. agalactiae, Salmonella, E. coli and P. aeruginosa. The bacteria of the genus Staphylococcus and Corynebacterium were resistant to penicillin (PEN), rifampicin (RIF), nitrofurantoin (NIT), erythromycin (ERI), and ciprofloxacin (CIP). The extract was used in vivo in the post-dipping of dairy cows, which reduced the microbiological load present in the mammary glands from 4.74 to 2.54 CFU, indicating its future use as an antiseptic.
    Keywords Corynebacterium ; Escherichia coli ; Eucalyptus ; Salmonella ; Staphylococcus ; agar ; antibiotic resistance ; antimicrobial properties ; antiseptics ; biochemical pathways ; bovine mastitis ; ciprofloxacin ; cytotoxicity ; dairy cattle ; erythromycin ; microbiology ; nitrofurantoin ; penicillins ; plate count ; rifampicin ; vinegars ; wood ; Antiseptic ; Antimicrobial ; Post-dipping ; Mastitis ; Eucalyptus urograndis
    Language English
    Dates of publication 2023-04
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2023.109670
    Database NAL-Catalogue (AGRICOLA)

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